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Protein

Type I inositol polyphosphate 5-phosphatase 12

Gene

IP5P12

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has phosphatase activity toward Ins(1,4,5)P3 (PubMed:15574849). Controls Ins(1,4,5)P3/Ca2+ levels that is crucial for maintaining pollen dormancy and regulating early germination of pollen (PubMed:22573619).2 Publications

Catalytic activityi

D-myo-inositol 1,4,5-trisphosphate + H2O = myo-inositol 1,4-bisphosphate + phosphate.1 Publication

Cofactori

Mg2+1 Publication

Kineticsi

  1. KM=223 µM for Ins(1,4,5)P31 Publication

    GO - Molecular functioni

    GO - Biological processi

    • phosphatidylinositol dephosphorylation Source: InterPro
    • pollen germination Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciARA:AT2G43900-MONOMER.
    MetaCyc:AT2G43900-MONOMER.
    BRENDAi3.1.3.56. 399.
    ReactomeiR-ATH-1660499. Synthesis of PIPs at the plasma membrane.
    R-ATH-1855183. Synthesis of IP2, IP, and Ins in the cytosol.
    R-ATH-1855204. Synthesis of IP3 and IP4 in the cytosol.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type I inositol polyphosphate 5-phosphatase 121 Publication (EC:3.1.3.561 Publication)
    Short name:
    At5PTase121 Publication
    Gene namesi
    Name:IP5P12Curated
    Synonyms:5PTASE121 Publication, IPP3
    Ordered Locus Names:At2g43900Imported
    ORF Names:F6E13.3Imported
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 2

    Organism-specific databases

    TAIRiAT2G43900.

    Pathology & Biotechi

    Disruption phenotypei

    Precocious pollen germination within anthers. Hypersensitivity to abscisic acid (ABA) during seed germination. Elevated levels of Ins(1,4,5)P3 and cytosolic Ca2+.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 13161316Type I inositol polyphosphate 5-phosphatase 12PRO_0000359742Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki946 – 946Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

    Keywords - PTMi

    Isopeptide bond, Ubl conjugation

    Proteomic databases

    PaxDbiO80560.
    PRIDEiO80560.

    PTM databases

    iPTMnetiO80560.

    Expressioni

    Tissue specificityi

    Mostly expressed in leaves and flowers and only weakly expressed in roots, stem and young seedlings. More precisely detected in cotyledon tips, hydathodes of leaves and mature pollen grains.1 Publication

    Inductioni

    Not induced by stresses.1 Publication

    Gene expression databases

    GenevisibleiO80560. AT.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT2G43900.1.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati153 – 19341WD 1Add
    BLAST
    Repeati213 – 25240WD 2Add
    BLAST
    Repeati264 – 30239WD 3Add
    BLAST
    Repeati441 – 48040WD 4Add
    BLAST
    Repeati520 – 55738WD 5Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni788 – 80417Catalytic 1By similarityAdd
    BLAST
    Regioni867 – 88216Catalytic 2By similarityAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi1103 – 1304202Ser-richPROSITE-ProRule annotationAdd
    BLAST
    Compositional biasi1109 – 1299191Lys-richPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 5 WD repeats.Curated

    Keywords - Domaini

    Repeat, WD repeat

    Phylogenomic databases

    eggNOGiKOG0565. Eukaryota.
    COG5411. LUCA.
    HOGENOMiHOG000242921.
    InParanoidiO80560.
    OMAiKFERHRP.
    OrthoDBiEOG093600RQ.

    Family and domain databases

    Gene3Di2.130.10.10. 2 hits.
    3.60.10.10. 2 hits.
    InterProiIPR005135. Endo/exonuclease/phosphatase.
    IPR000300. IPPc.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF03372. Exo_endo_phos. 1 hit.
    [Graphical view]
    SMARTiSM00128. IPPc. 1 hit.
    SM00320. WD40. 3 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 3 hits.
    SSF56219. SSF56219. 2 hits.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: O80560-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MDIINNNHRD ENDDDEEEAL SAMSSVPPPR KIHSYSHQLR ATGQKGHHRQ
    60 70 80 90 100
    RQHSLDDIPK ITEIVSGCGI SGDSSDDEFY PYATTTNSSS FPFTGGDTGD
    110 120 130 140 150
    SDDYLHQPEI GEDFQPLPEF VGSGGGVGMF KVPTRSPLHS ARPPCLELRP
    160 170 180 190 200
    HPLKETQVGR FLRNIACTET QLWAGQESGV RFWNFDDAFE PGCGLSGRVQ
    210 220 230 240 250
    RGDEDAAPFQ ESASTSPTTC LMVDNGNRLV WSGHKDGKIR SWKMDYVLDD
    260 270 280 290 300
    GDDSPFKEGL AWQAHKGPVN SVIMSSYGDL WSCSEGGVIK IWTWESMEKS
    310 320 330 340 350
    LSLRLEEKHM AALLVERSGI DLRAQVTVNG TCNISSSEVK CLLADNVRSK
    360 370 380 390 400
    VWAAQLQTFS LWDGRTKELL KVFNSEGQTE NRVDMPLGQD QPAAEDEMKA
    410 420 430 440 450
    KIASTSKKEK PHGFLQRSRN AIMGAADAVR RVATRGGGAY EDAKRTEAMV
    460 470 480 490 500
    LAGDGMIWTG CTNGLLIQWD GNGNRLQDFR HHQCAVLCFC TFGERIYIGY
    510 520 530 540 550
    VSGHIQIIDL EGNLIAGWVA HNNAVIKMAA ADGYIFSLAT HGGIRGWPVI
    560 570 580 590 600
    SPGPLDGIIR SELAEKERTY AQTDSVRILT GSWNVGQGKA SHDALMSWLG
    610 620 630 640 650
    SVASDVGILV VGLQEVEMGA GFLAMSAAKE SVGGNEGSTI GQYWIDTIGK
    660 670 680 690 700
    TLDEKAVFER MGSRQLAGLL ISLWVRKNLR THVGDIDVAA VPCGFGRAIG
    710 720 730 740 750
    NKGGVGLRIR VFDRIMCFIN CHLAAHLEAV NRRNADFDHI YKTMSFTRSS
    760 770 780 790 800
    NAHNAPAAGV STGSHTTKSA NNANVNTEET KQDLAEADMV VFFGDFNYRL
    810 820 830 840 850
    FGISYDEARD FVSQRSFDWL REKDQLRAEM KAGRVFQGMR EAIITFPPTY
    860 870 880 890 900
    KFERHRPGLG GYDSGEKKRI PAWCDRVIFR DTRTSPESEC SLDCPVVASI
    910 920 930 940 950
    MLYDACMDVT ESDHKPVRCK FHVKIEHVDR SVRRQEFGRI IKTNEKVRAL
    960 970 980 990 1000
    LNDLRYVPET IVSSNSIVLQ NQDTFVLRIT NKCVKENAVF RILCEGQSTV
    1010 1020 1030 1040 1050
    REDEDTLELH PLGSFGFPRW LEVMPAAGTI KPDSSVEVSV HHEEFHTLEE
    1060 1070 1080 1090 1100
    FVDGIPQNWW CEDTRDKEAI LVVNVQGGCS TETVCHRVHV RHCFSAKNLR
    1110 1120 1130 1140 1150
    IDSNPSNSKS QSLKKNEGDS NSKSSKKSDG DSNSKSSKKS DGDSNSKSSK
    1160 1170 1180 1190 1200
    KSDGDSNSKS SKKSDGDSNS KSSKKSDGDS NSKSSKKSDG DSNSKSSKKS
    1210 1220 1230 1240 1250
    DGDSCSKSQK KSDGDTNSKS QKKGDGDSSS KSHKKNDGDS SSKSHKKNDG
    1260 1270 1280 1290 1300
    DSSSKSHKKS DGDSSSKSHK KSEGDSSSKS HKKNDGDSSS SYKSQSGKKN
    1310
    SNSSTVEESR NNHNKR
    Length:1,316
    Mass (Da):144,731
    Last modified:June 24, 2015 - v2
    Checksum:iDD508A05B962A1A4
    GO
    Isoform 2 (identifier: O80560-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1220-1255: Missing.

    Note: No experimental confirmation available.
    Show »
    Length:1,280
    Mass (Da):140,983
    Checksum:iD36FEFCFB635775E
    GO

    Sequence cautioni

    The sequence AAC23399 differs from that shown. Reason: Frameshift at position 1278. Curated
    The sequence AAV87314 differs from that shown. Reason: Frameshift at position 1278. Curated
    The sequence AAV87318 differs from that shown. Reason: Frameshift at position 1278. Curated
    The sequence CAC81919 differs from that shown. Reason: Frameshift at positions 953 and 998. Curated

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti798 – 7981Y → N in CAC81919 (Ref. 2) Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1220 – 125536Missing in isoform 2. 1 PublicationVSP_036160Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY761187 mRNA. Translation: AAV87314.1. Frameshift.
    AY761191 Genomic DNA. Translation: AAV87318.1. Frameshift.
    AJ277884 mRNA. Translation: CAC81919.1. Frameshift.
    AC004005 Genomic DNA. Translation: AAC23399.1. Frameshift.
    CP002685 Genomic DNA. Translation: AEC10342.1.
    PIRiT00670.
    RefSeqiNP_181918.4. NM_129952.6. [O80560-1]
    UniGeneiAt.36825.

    Genome annotation databases

    EnsemblPlantsiAT2G43900.1; AT2G43900.1; AT2G43900. [O80560-1]
    GeneIDi818994.
    KEGGiath:AT2G43900.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY761187 mRNA. Translation: AAV87314.1. Frameshift.
    AY761191 Genomic DNA. Translation: AAV87318.1. Frameshift.
    AJ277884 mRNA. Translation: CAC81919.1. Frameshift.
    AC004005 Genomic DNA. Translation: AAC23399.1. Frameshift.
    CP002685 Genomic DNA. Translation: AEC10342.1.
    PIRiT00670.
    RefSeqiNP_181918.4. NM_129952.6. [O80560-1]
    UniGeneiAt.36825.

    3D structure databases

    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi3702.AT2G43900.1.

    PTM databases

    iPTMnetiO80560.

    Proteomic databases

    PaxDbiO80560.
    PRIDEiO80560.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT2G43900.1; AT2G43900.1; AT2G43900. [O80560-1]
    GeneIDi818994.
    KEGGiath:AT2G43900.

    Organism-specific databases

    TAIRiAT2G43900.

    Phylogenomic databases

    eggNOGiKOG0565. Eukaryota.
    COG5411. LUCA.
    HOGENOMiHOG000242921.
    InParanoidiO80560.
    OMAiKFERHRP.
    OrthoDBiEOG093600RQ.

    Enzyme and pathway databases

    BioCyciARA:AT2G43900-MONOMER.
    MetaCyc:AT2G43900-MONOMER.
    BRENDAi3.1.3.56. 399.
    ReactomeiR-ATH-1660499. Synthesis of PIPs at the plasma membrane.
    R-ATH-1855183. Synthesis of IP2, IP, and Ins in the cytosol.
    R-ATH-1855204. Synthesis of IP3 and IP4 in the cytosol.

    Miscellaneous databases

    PROiO80560.

    Gene expression databases

    GenevisibleiO80560. AT.

    Family and domain databases

    Gene3Di2.130.10.10. 2 hits.
    3.60.10.10. 2 hits.
    InterProiIPR005135. Endo/exonuclease/phosphatase.
    IPR000300. IPPc.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF03372. Exo_endo_phos. 1 hit.
    [Graphical view]
    SMARTiSM00128. IPPc. 1 hit.
    SM00320. WD40. 3 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 3 hits.
    SSF56219. SSF56219. 2 hits.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiIP5PC_ARATH
    AccessioniPrimary (citable) accession number: O80560
    Secondary accession number(s): F4IS64, Q712G3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 20, 2009
    Last sequence update: June 24, 2015
    Last modified: September 7, 2016
    This is version 118 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.