O80560 (IP5PC_ARATH) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 1, 2013.
Version 92.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Type I inositol 1,4,5-trisphosphate 5-phosphatase 12 Short name=At5PTase12 EC=3.1.3.56 | ||||||||
| Gene names |
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| Organism | Arabidopsis thaliana (Mouse-ear cress) [Reference proteome] | ||||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Protein attributes
| Sequence length | 1305 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Converts inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) to inositol 1,4-bisphosphate. Ref.1 |
| Catalytic activity | D-myo-inositol 1,4,5-trisphosphate + H2O = myo-inositol 1,4-bisphosphate + phosphate. 1D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O = 1D-myo-inositol 1,3,4-trisphosphate + phosphate. |
| Cofactor | Magnesium. Ref.1 |
| Induction | Not induced by stresses. Ref.1 |
| Sequence similarities | Belongs to the inositol 1,4,5-trisphosphate 5-phosphatase type I family. Contains 5 WD repeats. |
| Biophysicochemical properties | Kinetic parameters: KM=223 µM for Ins(1,4,5)P3 Ref.1 |
| Sequence caution | The sequence AEC10342.1 differs from that shown. Reason: Frameshift at position 1278. The sequence CAC81919.1 differs from that shown. Reason: Frameshift at positions 953 and 998. |
Ontologies
| Keywords | |
|---|---|
| Coding sequence diversity | Alternative splicing |
| Domain | Repeat WD repeat |
| Ligand | Magnesium Metal-binding |
| Molecular function | Hydrolase |
| PTM | Isopeptide bond Ubl conjugation |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological_process | phosphatidylinositol phosphorylation Inferred from electronic annotation. Source: InterPro |
| Molecular_function | inositol-1,3,4,5-tetrakisphosphate 5-phosphatase activity Inferred from electronic annotation. Source: EC inositol-1,4,5-trisphosphate 5-phosphatase activityInferred from electronic annotation. Source: EC inositol-polyphosphate 5-phosphatase activityInferred from electronic annotation. Source: EC metal ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: O80560-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: O80560-2) The sequence of this isoform differs from the canonical sequence as follows: 1221-1221: Q → H 1224-1224: G → S 1236-1237: ND → SE 1255-1280: KSHKKSDGDSSSKSHKKSEGDSSLSL → SYKSQSGKKNSNSSTVEESRNNHNKR 1281-1305: Missing. | ||||||
| Note: No experimental confirmation available. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1305 | 1305 | Type I inositol 1,4,5-trisphosphate 5-phosphatase 12 | PRO_0000359742 | |||||
Regions | |||||||||
| Repeat | 153 – 193 | 41 | WD 1 | ||||||
| Repeat | 213 – 252 | 40 | WD 2 | ||||||
| Repeat | 264 – 302 | 39 | WD 3 | ||||||
| Repeat | 441 – 480 | 40 | WD 4 | ||||||
| Repeat | 520 – 557 | 38 | WD 5 | ||||||
| Compositional bias | 1109 – 1271 | 163 | Lys-rich | ||||||
Amino acid modifications | |||||||||
| Cross-link | 946 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.5 | |||||||
Natural variations | |||||||||
| Alternative sequence | 1221 | 1 | Q → H in isoform 2. | VSP_036156 | |||||
| Alternative sequence | 1224 | 1 | G → S in isoform 2. | VSP_036157 | |||||
| Alternative sequence | 1236 – 1237 | 2 | ND → SE in isoform 2. | VSP_036158 | |||||
| Alternative sequence | 1255 – 1280 | 26 | KSHKK…SSLSL → SYKSQSGKKNSNSSTVEESR NNHNKR in isoform 2. | VSP_036159 | |||||
| Alternative sequence | 1281 – 1305 | 25 | Missing in isoform 2. | VSP_036160 | |||||
Experimental info | |||||||||
| Sequence conflict | 798 | 1 | Y → N in CAC81919. Ref.2 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Molecular and biochemical characterization of three WD-repeat-domain-containing inositol polyphosphate 5-phosphatases in Arabidopsis thaliana." Zhong R., Ye Z.-H. Plant Cell Physiol. 45:1720-1728(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, INDUCTION, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES. Strain: cv. Columbia. |
| [2] | Lin W.-H., Xu Z.-H., Mueller-Roeber B., Xue H.-W. Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Hypocotyl. |
| [3] | "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A.  Venter J.C.Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [4] | The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia. |
| [5] | "Multidimensional protein identification technology (MudPIT) analysis of ubiquitinated proteins in plants." Maor R., Jones A., Nuehse T.S., Studholme D.J., Peck S.C., Shirasu K. Mol. Cell. Proteomics 6:601-610(2007) [PubMed] [Europe PMC] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-946, MASS SPECTROMETRY. Strain: cv. Landsberg erecta. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AY761187 mRNA. Translation: AAV87314.1. AY761191 Genomic DNA. Translation: AAV87318.1. AJ277884 mRNA. Translation: CAC81919.1. Frameshift. AC004005 Genomic DNA. Translation: AAC23399.1. CP002685 Genomic DNA. Translation: AEC10342.1. Frameshift. |
| IPI | IPI00534495. IPI00919332. |
| PIR | T00670. |
| RefSeq | NP_181918.4. NM_129952.6. |
| UniGene | At.36825. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1I9Z based on UniProtKB O43001. |
| ProteinModelPortal | O80560. |
| SMR | O80560. Positions 230-295, 513-550, 574-925. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | 3702.AT2G43900.1-P. |
Proteomic databases | |
| PaxDb | O80560. |
| PRIDE | O80560. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| GeneID | 818994. |
| KEGG | ath:AT2G43900. |
Organism-specific databases | |
| GeneFarm | 3134. 267. |
| TAIR | At2g43900. |
Phylogenomic databases | |
| eggNOG | COG5411. |
| HOGENOM | HOG000242921. |
| InParanoid | O80560. |
| PhylomeDB | O80560. |
Enzyme and pathway databases | |
| BioCyc | MetaCyc:AT2G43900-MONOMER. |
Gene expression databases | |
| ArrayExpress | O80560. |
| Genevestigator | O80560. |
Family and domain databases | |
| Gene3D | 2.130.10.10. 2 hits. |
| InterPro | IPR005135. Endo/exonuclease/phosphatase. IPR000300. IPPc. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. [Graphical view] |
| Pfam | PF03372. Exo_endo_phos. 1 hit. [Graphical view] |
| SMART | SM00128. IPPc. 1 hit. SM00320. WD40. 3 hits. [Graphical view] |
| SUPFAM | SSF56219. Exo_endo_phos. 1 hit. |
| PROSITE | PS00678. WD_REPEATS_1. False negative. PS50082. WD_REPEATS_2. False negative. PS50294. WD_REPEATS_REGION. False negative. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | IP5PC_ARATH | ||||||||
| Accession | Primary (citable) accession number: O80560 Secondary accession number(s): F4IS64, Q712G3 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Plant Protein Annotation Program | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| SIMILARITY comments Index of protein domains and families |