Reviewed,
UniProtKB/Swiss-Prot O80560 (IP5PC_ARATH)
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November 3, 2009.
Version 65.
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: Type I inositol-1,4,5-trisphosphate 5-phosphatase 12 Short name=At5PTase12 EC=3.1.3.56 | ||||||||
| Gene names |
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| Organism | Arabidopsis thaliana (Mouse-ear cress) [Complete proteome] | ||||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis |
Protein attributes
| Sequence length | 1305 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Converts inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) to inositol 1,4-bisphosphate. Ref.1 |
| Catalytic activity | D-myo-inositol 1,4,5-trisphosphate + H2O = myo-inositol 1,4-bisphosphate + phosphate. 1D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O = 1D-myo-inositol 1,3,4-trisphosphate + phosphate. |
| Cofactor | Magnesium. Ref.1 |
| Induction | Not induced by stresses. Ref.1 |
| Sequence similarities | Belongs to the inositol-1,4,5-trisphosphate 5-phosphatase type I family. Contains 5 WD repeats. |
| Biophysicochemical properties | Kinetic parameters: KM=223 µM for Ins(1,4,5)P3 |
| Sequence caution | The sequence CAC81919.1 differs from that shown. Reason: Frameshift at positions 953 and 998. |
Ontologies
| Keywords | |
|---|---|
| Coding sequence diversity | Alternative splicing |
| Domain | Repeat WD repeat |
| Ligand | Magnesium Metal-binding |
| Molecular function | Hydrolase |
| PTM | Isopeptide bond Ubl conjugation |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Molecular function | inositol-polyphosphate 5-phosphatase activity Inferred from electronic annotation. Source: EC magnesium ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: O80560-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: O80560-2) The sequence of this isoform differs from the canonical sequence as follows: 1221-1221: Q → H 1224-1224: G → S 1236-1237: ND → SE 1255-1280: KSHKKSDGDSSSKSHKKSEGDSSLSL → SYKSQSGKKNSNSSTVEESRNNHNKR 1281-1305: Missing. | ||||||
| Note: No experimental confirmation available. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1305 | 1305 | Type I inositol-1,4,5-trisphosphate 5-phosphatase 12 | PRO_0000359742 | |||||
Regions | |||||||||
| Repeat | 153 – 193 | 41 | WD 1 | ||||||
| Repeat | 213 – 252 | 40 | WD 2 | ||||||
| Repeat | 264 – 302 | 39 | WD 3 | ||||||
| Repeat | 441 – 480 | 40 | WD 4 | ||||||
| Repeat | 520 – 557 | 38 | WD 5 | ||||||
| Compositional bias | 1109 – 1271 | 163 | Lys-rich | ||||||
Amino acid modifications | |||||||||
| Cross-link | 946 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.4 | |||||||
Natural variations | |||||||||
| Alternative sequence | 1221 | 1 | Q → H in isoform 2. | VSP_036156 | |||||
| Alternative sequence | 1224 | 1 | G → S in isoform 2. | VSP_036157 | |||||
| Alternative sequence | 1236 – 1237 | 2 | ND → SE in isoform 2. | VSP_036158 | |||||
| Alternative sequence | 1255 – 1280 | 26 | KSHKK…SSLSL → SYKSQSGKKNSNSSTVEESR NNHNKR in isoform 2. | VSP_036159 | |||||
| Alternative sequence | 1281 – 1305 | 25 | Missing in isoform 2. | VSP_036160 | |||||
Experimental info | |||||||||
| Sequence conflict | 798 | 1 | Y → N in CAC81919. Ref.2 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Molecular and biochemical characterization of three WD-repeat-domain-containing inositol polyphosphate 5-phosphatases in Arabidopsis thaliana." Zhong R., Ye Z.-H. Plant Cell Physiol. 45:1720-1728(2004) [PubMed: 15574849] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, INDUCTION, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES. Strain: cv. Columbia. |
| [2] | Lin W.-H., Xu Z.-H., Mueller-Roeber B., Xue H.-W. Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Hypocotyl. |
| [3] | "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A.  Venter J.C.Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [4] | "Multidimensional protein identification technology (MudPIT) analysis of ubiquitinated proteins in plants." Maor R., Jones A., Nuehse T.S., Studholme D.J., Peck S.C., Shirasu K. Mol. Cell. Proteomics 6:601-610(2007) [PubMed: 17272265] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-946, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| AY761187 mRNA. Translation: AAV87314.1. AY761191 Genomic DNA. Translation: AAV87318.1. AJ277884 mRNA. Translation: CAC81919.1. Frameshift. AC004005 Genomic DNA. Translation: AAC23399.1. | |
| IPI | IPI00534495. IPI00919332. |
| PIR | T00670. |
| UniGene | At.36825 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1I9Z based on UniProtKB O43001. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | O80560. |
Proteomic databases | |
| PRIDE | O80560. |
Genome annotation databases | |
| GenomeReviews | Gene locus AT2G43900 in contig CT485783_GR. |
| KEGG | ath:AT2G43900. |
| NMPDR | fig|3702.1.peg.11553. |
Organism-specific databases | |
| GeneFarm | 3134. 267. |
| TAIR | At2g43900. |
Phylogenomic databases | |
| OMA | SKKSDGD. |
Gene expression databases | |
| Genevestigator | O80560. |
Family and domain databases | |
| InterPro | IPR005135. Endo/exonuclease/phosphatase. IPR000300. IPPc. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR019775. WD40_repeat_CS. [Graphical view] |
| Gene3D | G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit. |
| Pfam | PF03372. Exo_endo_phos. 1 hit. [Graphical view] |
| SMART | SM00128. IPPc. 1 hit. SM00320. WD40. 3 hits. [Graphical view] |
| PROSITE | PS00678. WD_REPEATS_1. False negative. PS50082. WD_REPEATS_2. False negative. PS50294. WD_REPEATS_REGION. False negative. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | IP5PC_ARATH | ||||||||
| Accession | Primary (citable) accession number: O80560 Secondary accession number(s): Q712G3 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| SIMILARITY comments Index of protein domains and families |
