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Protein

Transcription factor PIF3

Gene

PIF3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor acting positively in the phytochrome signaling pathway. Activates transcription by binding to the G box (5'-CACGTG-3').2 Publications

GO - Molecular functioni

  • DNA binding Source: TAIR
  • transcription factor activity, sequence-specific DNA binding Source: TAIR

GO - Biological processi

  • de-etiolation Source: TAIR
  • gibberellic acid mediated signaling pathway Source: TAIR
  • positive regulation of anthocyanin metabolic process Source: TAIR
  • red, far-red light phototransduction Source: UniProtKB-KW
  • red or far-red light signaling pathway Source: TAIR
  • regulation of transcription, DNA-templated Source: TAIR
  • response to red or far red light Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Phytochrome signaling pathway, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor PIF3
Alternative name(s):
Basic helix-loop-helix protein 8
Short name:
AtbHLH8
Short name:
bHLH 8
Phytochrome-associated protein 3
Phytochrome-interacting factor 3
Transcription factor EN 100
bHLH transcription factor bHLH008
Gene namesi
Name:PIF3
Synonyms:BHLH8, EN100, PAP3
Ordered Locus Names:At1g09530
ORF Names:F14J9.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G09530.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001274281 – 524Transcription factor PIF3Add BLAST524

Proteomic databases

PaxDbiO80536.

Expressioni

Inductioni

By UV treatment.1 Publication

Gene expression databases

GenevisibleiO80536. AT.

Interactioni

Subunit structurei

Homodimer (Probable). Can form a heterodimer with REP1 and PIF4. Phytochrome B binds specifically to DNA-bound PIF3, but only upon red light induced conversion to the Pfr form (PfrB). Reconversion to Pr form causes rapid dissociation. Interacts with APRR1/TOC1.Curated3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-625701,EBI-625701
HFR1Q9FE226EBI-625701,EBI-626001
PHYAP147128EBI-625701,EBI-624446
PHYBP1471319EBI-625701,EBI-300727
PIF1Q8GZM76EBI-625701,EBI-630400
PIF4Q8W2F3-23EBI-625701,EBI-625732

Protein-protein interaction databases

BioGridi22720. 30 interactors.
DIPiDIP-33892N.
IntActiO80536. 12 interactors.
MINTiMINT-8058904.
STRINGi3702.AT1G09530.1.

Structurei

3D structure databases

ProteinModelPortaliO80536.
SMRiO80536.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini343 – 392bHLHPROSITE-ProRule annotationAdd BLAST50

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi353 – 358Poly-Arg6
Compositional biasi422 – 428Poly-Ala7

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IV8V. Eukaryota.
ENOG4111SYS. LUCA.
InParanoidiO80536.
KOiK12126.
OMAiHFLRPAT.
OrthoDBiEOG093605W3.
PhylomeDBiO80536.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O80536-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLFELFRLT KAKLESAQDR NPSPPVDEVV ELVWENGQIS TQSQSSRSRN
60 70 80 90 100
IPPPQANSSR AREIGNGSKT TMVDEIPMSV PSLMTGLSQD DDFVPWLNHH
110 120 130 140 150
PSLDGYCSDF LRDVSSPVTV NEQESDMAVN QTAFPLFQRR KDGNESAPAA
160 170 180 190 200
SSSQYNGFQS HSLYGSDRAR DLPSQQTNPD RFTQTQEPLI TSNKPSLVNF
210 220 230 240 250
SHFLRPATFA KTTNNNLHDT KEKSPQSPPN VFQTRVLGAK DSEDKVLNES
260 270 280 290 300
VASATPKDNQ KACLISEDSC RKDQESEKAV VCSSVGSGNS LDGPSESPSL
310 320 330 340 350
SLKRKHSNIQ DIDCHSEDVE EESGDGRKEA GPSRTGLGSK RSRSAEVHNL
360 370 380 390 400
SERRRRDRIN EKMRALQELI PNCNKVDKAS MLDEAIEYLK SLQLQVQIMS
410 420 430 440 450
MASGYYLPPA VMFPPGMGHY PAAAAAMAMG MGMPYAMGLP DLSRGGSSVN
460 470 480 490 500
HGPQFQVSGM QQQPVAMGIP RVSGGGIFAG SSTIGNGSTR DLSGSKDQTT
510 520
TNNNSNLKPI KRKQGSSDQF CGSS
Length:524
Mass (Da):56,990
Last modified:November 1, 1998 - v1
Checksum:i1044AC01D598DE7C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti15E → D in AAC99771 (Ref. 3) Curated1
Sequence conflicti344S → L in AAC99771 (Ref. 3) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti124E → D in strain: cv. An-2 and cv. Su-0. 1 Publication1
Natural varianti141K → N in strain: cv. An-2 and cv. Su-0. 1 Publication1
Natural varianti176Q → H in strain: cv. An-2 and cv. Su-0. 1 Publication1
Natural varianti186Q → K in strain: cv. An-2 and cv. Su-0. 1 Publication1
Natural varianti209F → L in strain: cv. An-2 and cv. Su-0. 1 Publication1
Natural varianti220T → I in strain: cv. An-2 and cv. Su-0. 1 Publication1
Natural varianti236V → I in strain: cv. An-2 and cv. Su-0. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF100166 mRNA. Translation: AAC95156.1.
AF251693 mRNA. Translation: AAL55715.1.
AF088280 mRNA. Translation: AAC99771.1.
AC003970 Genomic DNA. Translation: AAC33213.1.
CP002684 Genomic DNA. Translation: AEE28457.1.
CP002684 Genomic DNA. Translation: AEE28458.1.
AK117255 mRNA. Translation: BAC41930.1.
EF193482 Genomic DNA. Translation: ABP96435.1.
EF193483 Genomic DNA. Translation: ABP96436.1.
EF193484 Genomic DNA. Translation: ABP96437.1.
EF193485 Genomic DNA. Translation: ABP96438.1.
EF193486 Genomic DNA. Translation: ABP96439.1.
EF193487 Genomic DNA. Translation: ABP96440.1.
EF193488 Genomic DNA. Translation: ABP96441.1.
EF193489 Genomic DNA. Translation: ABP96442.1.
EF193490 Genomic DNA. Translation: ABP96443.1.
EF193491 Genomic DNA. Translation: ABP96444.1.
EF193492 Genomic DNA. Translation: ABP96445.1.
EF193493 Genomic DNA. Translation: ABP96446.1.
EF193494 Genomic DNA. Translation: ABP96447.1.
EF193495 Genomic DNA. Translation: ABP96448.1.
EF193496 Genomic DNA. Translation: ABP96449.1.
PIRiH86228.
RefSeqiNP_172424.1. NM_100824.3.
NP_849626.1. NM_179295.3.
UniGeneiAt.10926.

Genome annotation databases

EnsemblPlantsiAT1G09530.1; AT1G09530.1; AT1G09530.
AT1G09530.2; AT1G09530.2; AT1G09530.
GeneIDi837479.
GrameneiAT1G09530.1; AT1G09530.1; AT1G09530.
AT1G09530.2; AT1G09530.2; AT1G09530.
KEGGiath:AT1G09530.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF100166 mRNA. Translation: AAC95156.1.
AF251693 mRNA. Translation: AAL55715.1.
AF088280 mRNA. Translation: AAC99771.1.
AC003970 Genomic DNA. Translation: AAC33213.1.
CP002684 Genomic DNA. Translation: AEE28457.1.
CP002684 Genomic DNA. Translation: AEE28458.1.
AK117255 mRNA. Translation: BAC41930.1.
EF193482 Genomic DNA. Translation: ABP96435.1.
EF193483 Genomic DNA. Translation: ABP96436.1.
EF193484 Genomic DNA. Translation: ABP96437.1.
EF193485 Genomic DNA. Translation: ABP96438.1.
EF193486 Genomic DNA. Translation: ABP96439.1.
EF193487 Genomic DNA. Translation: ABP96440.1.
EF193488 Genomic DNA. Translation: ABP96441.1.
EF193489 Genomic DNA. Translation: ABP96442.1.
EF193490 Genomic DNA. Translation: ABP96443.1.
EF193491 Genomic DNA. Translation: ABP96444.1.
EF193492 Genomic DNA. Translation: ABP96445.1.
EF193493 Genomic DNA. Translation: ABP96446.1.
EF193494 Genomic DNA. Translation: ABP96447.1.
EF193495 Genomic DNA. Translation: ABP96448.1.
EF193496 Genomic DNA. Translation: ABP96449.1.
PIRiH86228.
RefSeqiNP_172424.1. NM_100824.3.
NP_849626.1. NM_179295.3.
UniGeneiAt.10926.

3D structure databases

ProteinModelPortaliO80536.
SMRiO80536.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi22720. 30 interactors.
DIPiDIP-33892N.
IntActiO80536. 12 interactors.
MINTiMINT-8058904.
STRINGi3702.AT1G09530.1.

Proteomic databases

PaxDbiO80536.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G09530.1; AT1G09530.1; AT1G09530.
AT1G09530.2; AT1G09530.2; AT1G09530.
GeneIDi837479.
GrameneiAT1G09530.1; AT1G09530.1; AT1G09530.
AT1G09530.2; AT1G09530.2; AT1G09530.
KEGGiath:AT1G09530.

Organism-specific databases

TAIRiAT1G09530.

Phylogenomic databases

eggNOGiENOG410IV8V. Eukaryota.
ENOG4111SYS. LUCA.
InParanoidiO80536.
KOiK12126.
OMAiHFLRPAT.
OrthoDBiEOG093605W3.
PhylomeDBiO80536.

Miscellaneous databases

PROiO80536.

Gene expression databases

GenevisibleiO80536. AT.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPIF3_ARATH
AccessioniPrimary (citable) accession number: O80536
Secondary accession number(s): A5Y7A2
, A5Y7A3, A5Y7A4, A5Y7A5, A5Y7A6, A5Y7A7, A5Y7A8, A5Y7A9, A5Y7B0, A5Y7B1, A5Y7B2, A5Y7B3, A5Y7B4, A5Y7B5, A5Y7B6, Q9SBC5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2002
Last sequence update: November 1, 1998
Last modified: November 30, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.