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Protein

Transcription factor PIF3

Gene

PIF3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor acting positively in the phytochrome signaling pathway. Activates transcription by binding to the G box (5'-CACGTG-3').2 Publications

GO - Molecular functioni

  • DNA binding Source: TAIR
  • DNA binding transcription factor activity Source: TAIR
  • identical protein binding Source: IntAct
  • protein dimerization activity Source: InterPro

GO - Biological processi

  • de-etiolation Source: TAIR
  • gibberellic acid mediated signaling pathway Source: TAIR
  • positive regulation of anthocyanin metabolic process Source: TAIR
  • red, far-red light phototransduction Source: UniProtKB-KW
  • red or far-red light signaling pathway Source: TAIR
  • regulation of transcription, DNA-templated Source: TAIR
  • response to red or far red light Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding
Biological processPhytochrome signaling pathway, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor PIF3
Alternative name(s):
Basic helix-loop-helix protein 8
Short name:
AtbHLH8
Short name:
bHLH 8
Phytochrome-associated protein 3
Phytochrome-interacting factor 3
Transcription factor EN 100
bHLH transcription factor bHLH008
Gene namesi
Name:PIF3
Synonyms:BHLH8, EN100, PAP3
Ordered Locus Names:At1g09530
ORF Names:F14J9.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G09530
TAIRilocus:2012345 AT1G09530

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001274281 – 524Transcription factor PIF3Add BLAST524

Post-translational modificationi

Phosphorylated by PHYA; this phosphorylation is repressed by PIA2.1 Publication
Dephosphorylated by TOPP4 during photomorphogenesis, leading to subsequent degradation of PIF3 by the proteasomal pathway.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO80536
PRIDEiO80536

Expressioni

Inductioni

By UV treatment.1 Publication

Gene expression databases

ExpressionAtlasiO80536 baseline and differential
GenevisibleiO80536 AT

Interactioni

Subunit structurei

Homodimer (Probable). Can form a heterodimer with REP1 and PIF4. Phytochrome B binds specifically to DNA-bound PIF3, but only upon red light induced conversion to the Pfr form (PfrB). Reconversion to Pr form causes rapid dissociation. Interacts with APRR1/TOC1. Binds to PIA2; this interaction may trigger the repression of PHYA-mediated phosphorylation (PubMed:27143545). Interacts with TOPP4 (PubMed:26704640).Curated5 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • protein dimerization activity Source: InterPro

Protein-protein interaction databases

BioGridi22720, 33 interactors
DIPiDIP-33892N
IntActiO80536, 18 interactors
STRINGi3702.AT1G09530.1

Structurei

3D structure databases

ProteinModelPortaliO80536
SMRiO80536
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini343 – 392bHLHPROSITE-ProRule annotationAdd BLAST50

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi353 – 358Poly-Arg6
Compositional biasi422 – 428Poly-Ala7

Phylogenomic databases

eggNOGiENOG410IV8V Eukaryota
ENOG4111SYS LUCA
InParanoidiO80536
KOiK12126
OMAiVACSSVC
OrthoDBiEOG093605W3
PhylomeDBiO80536

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
PfamiView protein in Pfam
PF00010 HLH, 1 hit
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit

Sequencei

Sequence statusi: Complete.

O80536-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLFELFRLT KAKLESAQDR NPSPPVDEVV ELVWENGQIS TQSQSSRSRN
60 70 80 90 100
IPPPQANSSR AREIGNGSKT TMVDEIPMSV PSLMTGLSQD DDFVPWLNHH
110 120 130 140 150
PSLDGYCSDF LRDVSSPVTV NEQESDMAVN QTAFPLFQRR KDGNESAPAA
160 170 180 190 200
SSSQYNGFQS HSLYGSDRAR DLPSQQTNPD RFTQTQEPLI TSNKPSLVNF
210 220 230 240 250
SHFLRPATFA KTTNNNLHDT KEKSPQSPPN VFQTRVLGAK DSEDKVLNES
260 270 280 290 300
VASATPKDNQ KACLISEDSC RKDQESEKAV VCSSVGSGNS LDGPSESPSL
310 320 330 340 350
SLKRKHSNIQ DIDCHSEDVE EESGDGRKEA GPSRTGLGSK RSRSAEVHNL
360 370 380 390 400
SERRRRDRIN EKMRALQELI PNCNKVDKAS MLDEAIEYLK SLQLQVQIMS
410 420 430 440 450
MASGYYLPPA VMFPPGMGHY PAAAAAMAMG MGMPYAMGLP DLSRGGSSVN
460 470 480 490 500
HGPQFQVSGM QQQPVAMGIP RVSGGGIFAG SSTIGNGSTR DLSGSKDQTT
510 520
TNNNSNLKPI KRKQGSSDQF CGSS
Length:524
Mass (Da):56,990
Last modified:November 1, 1998 - v1
Checksum:i1044AC01D598DE7C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti15E → D in AAC99771 (Ref. 3) Curated1
Sequence conflicti344S → L in AAC99771 (Ref. 3) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti124E → D in strain: cv. An-2 and cv. Su-0. 1 Publication1
Natural varianti141K → N in strain: cv. An-2 and cv. Su-0. 1 Publication1
Natural varianti176Q → H in strain: cv. An-2 and cv. Su-0. 1 Publication1
Natural varianti186Q → K in strain: cv. An-2 and cv. Su-0. 1 Publication1
Natural varianti209F → L in strain: cv. An-2 and cv. Su-0. 1 Publication1
Natural varianti220T → I in strain: cv. An-2 and cv. Su-0. 1 Publication1
Natural varianti236V → I in strain: cv. An-2 and cv. Su-0. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF100166 mRNA Translation: AAC95156.1
AF251693 mRNA Translation: AAL55715.1
AF088280 mRNA Translation: AAC99771.1
AC003970 Genomic DNA Translation: AAC33213.1
CP002684 Genomic DNA Translation: AEE28457.1
CP002684 Genomic DNA Translation: AEE28458.1
CP002684 Genomic DNA Translation: ANM60410.1
CP002684 Genomic DNA Translation: ANM60411.1
CP002684 Genomic DNA Translation: ANM60412.1
CP002684 Genomic DNA Translation: ANM60413.1
AK117255 mRNA Translation: BAC41930.1
EF193482 Genomic DNA Translation: ABP96435.1
EF193483 Genomic DNA Translation: ABP96436.1
EF193484 Genomic DNA Translation: ABP96437.1
EF193485 Genomic DNA Translation: ABP96438.1
EF193486 Genomic DNA Translation: ABP96439.1
EF193487 Genomic DNA Translation: ABP96440.1
EF193488 Genomic DNA Translation: ABP96441.1
EF193489 Genomic DNA Translation: ABP96442.1
EF193490 Genomic DNA Translation: ABP96443.1
EF193491 Genomic DNA Translation: ABP96444.1
EF193492 Genomic DNA Translation: ABP96445.1
EF193493 Genomic DNA Translation: ABP96446.1
EF193494 Genomic DNA Translation: ABP96447.1
EF193495 Genomic DNA Translation: ABP96448.1
EF193496 Genomic DNA Translation: ABP96449.1
PIRiH86228
RefSeqiNP_001318964.1, NM_001331836.1
NP_001318965.1, NM_001331837.1
NP_001322700.1, NM_001331838.1
NP_001322701.1, NM_001331839.1
NP_172424.1, NM_100824.3
NP_849626.1, NM_179295.3
UniGeneiAt.10926

Genome annotation databases

EnsemblPlantsiAT1G09530.1; AT1G09530.1; AT1G09530
AT1G09530.2; AT1G09530.2; AT1G09530
AT1G09530.3; AT1G09530.3; AT1G09530
AT1G09530.4; AT1G09530.4; AT1G09530
AT1G09530.5; AT1G09530.5; AT1G09530
AT1G09530.6; AT1G09530.6; AT1G09530
GeneIDi837479
GrameneiAT1G09530.1; AT1G09530.1; AT1G09530
AT1G09530.2; AT1G09530.2; AT1G09530
AT1G09530.3; AT1G09530.3; AT1G09530
AT1G09530.4; AT1G09530.4; AT1G09530
AT1G09530.5; AT1G09530.5; AT1G09530
AT1G09530.6; AT1G09530.6; AT1G09530
KEGGiath:AT1G09530

Similar proteinsi

Entry informationi

Entry nameiPIF3_ARATH
AccessioniPrimary (citable) accession number: O80536
Secondary accession number(s): A5Y7A2
, A5Y7A3, A5Y7A4, A5Y7A5, A5Y7A6, A5Y7A7, A5Y7A8, A5Y7A9, A5Y7B0, A5Y7B1, A5Y7B2, A5Y7B3, A5Y7B4, A5Y7B5, A5Y7B6, Q9SBC5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2002
Last sequence update: November 1, 1998
Last modified: May 23, 2018
This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names

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