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Protein

ATP-citrate synthase alpha chain protein 3

Gene

ACLA-3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA, used for the elongation of fatty acids and biosynthesis of isoprenoids, flavonoids and malonated derivatives. May supply substrate to the cytosolic acetyl-CoA carboxylase, which generates the malonyl-CoA used for the synthesis of a multitude of compounds, including very long chain fatty acids and flavonoids. Required for normal growth and development and elongation of C18 fatty acids to C20 to C24 fatty acids in seeds. In contrast to all known animal ACL enzymes having a homomeric structure, plant ACLs are composed of alpha and beta chains (By similarity).By similarity

Catalytic activityi

ADP + phosphate + acetyl-CoA + oxaloacetate = ATP + citrate + CoA.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei343Citrate; via amide nitrogenBy similarity1
Binding sitei345CitrateBy similarity1
Binding sitei376CitrateBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP citrate synthase activity Source: TAIR

GO - Biological processi

  • acetyl-CoA biosynthetic process Source: TAIR
  • fatty acid biosynthetic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-citrate synthase alpha chain protein 3 (EC:2.3.3.8)
Short name:
ATP-citrate synthase A-3
Alternative name(s):
ATP-citrate lyase A-3
Citrate cleavage enzyme A-3
Gene namesi
Name:ACLA-3
Ordered Locus Names:At1g09430
ORF Names:F19J9.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G09430.

Subcellular locationi

GO - Cellular componenti

  • citrate lyase complex Source: TAIR
  • cytosol Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004122171 – 424ATP-citrate synthase alpha chain protein 3Add BLAST424

Proteomic databases

PaxDbiO80526.
PRIDEiO80526.

PTM databases

iPTMnetiO80526.

Expressioni

Gene expression databases

GenevisibleiO80526. AT.

Interactioni

Subunit structurei

Heterooctamer of 4 alpha and 4 beta chains.By similarity

Protein-protein interaction databases

BioGridi22707. 1 interactor.
IntActiO80526. 1 interactor.
STRINGi3702.AT1G09430.1.

Structurei

3D structure databases

ProteinModelPortaliO80526.
SMRiO80526.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1254. Eukaryota.
COG0045. LUCA.
HOGENOMiHOG000151490.
InParanoidiO80526.
KOiK01648.
OMAiEKQMTPD.
OrthoDBiEOG09360AMI.
PhylomeDBiO80526.

Family and domain databases

Gene3Di3.30.470.20. 1 hit.
3.40.50.261. 1 hit.
InterProiIPR013650. ATP-grasp_succ-CoA_synth-type.
IPR013816. ATP_grasp_subdomain_2.
IPR032263. Citrate-bd.
IPR016102. Succinyl-CoA_synth-like.
[Graphical view]
PfamiPF08442. ATP-grasp_2. 1 hit.
PF16114. Citrate_bind. 1 hit.
[Graphical view]
SUPFAMiSSF52210. SSF52210. 1 hit.

Sequencei

Sequence statusi: Complete.

O80526-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARKKIREYD SKRLLKEHLK RLANIDLQIR SAQVTESTDF TELTNQESWL
60 70 80 90 100
SSTKLVVKPD MLFGKRGKSG LVALKLDLAE VADFVKARLG TEVEMEGCKA
110 120 130 140 150
PITTFIVEPF VPHDQEYYLS IVSDRLGCTI SFSECGGIEI EENWDKVKTI
160 170 180 190 200
FLPAEKSMTL EVCAPLIATL PLEVRAKIGN FIMGAFAVFQ DLDFSFMEMN
210 220 230 240 250
PFTLVDGEPF PLDMRGELDD TAAFKNFNKW GDIEFPLPFG RVLSSTENFI
260 270 280 290 300
HGLDEKTSAS LKFTVLNPKG RIWTMVAGGG ASVIYADTVG DLGYASELGN
310 320 330 340 350
YAEYSGAPNE EEVLQYARVV IDCATTDPDG RKRALLIGGG IANFTDVAAT
360 370 380 390 400
FNGIIRALRE KETRLKASRM HIYVRRGGPN YQTGLARMRA LGEELGVPLE
410 420
VYGPEATMTG ICKRAIDCIM LPDA
Length:424
Mass (Da):46,946
Last modified:November 1, 1998 - v1
Checksum:iFE9AFB8E6CB67389
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC003970 Genomic DNA. Translation: AAC33203.1.
CP002684 Genomic DNA. Translation: AEE28442.1.
AY127019 mRNA. Translation: AAM83243.1.
BT003017 mRNA. Translation: AAO23582.1.
PIRiF86227.
RefSeqiNP_172414.1. NM_100814.4.
UniGeneiAt.23527.
At.66837.

Genome annotation databases

EnsemblPlantsiAT1G09430.1; AT1G09430.1; AT1G09430.
GeneIDi837466.
GrameneiAT1G09430.1; AT1G09430.1; AT1G09430.
KEGGiath:AT1G09430.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC003970 Genomic DNA. Translation: AAC33203.1.
CP002684 Genomic DNA. Translation: AEE28442.1.
AY127019 mRNA. Translation: AAM83243.1.
BT003017 mRNA. Translation: AAO23582.1.
PIRiF86227.
RefSeqiNP_172414.1. NM_100814.4.
UniGeneiAt.23527.
At.66837.

3D structure databases

ProteinModelPortaliO80526.
SMRiO80526.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi22707. 1 interactor.
IntActiO80526. 1 interactor.
STRINGi3702.AT1G09430.1.

PTM databases

iPTMnetiO80526.

Proteomic databases

PaxDbiO80526.
PRIDEiO80526.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G09430.1; AT1G09430.1; AT1G09430.
GeneIDi837466.
GrameneiAT1G09430.1; AT1G09430.1; AT1G09430.
KEGGiath:AT1G09430.

Organism-specific databases

TAIRiAT1G09430.

Phylogenomic databases

eggNOGiKOG1254. Eukaryota.
COG0045. LUCA.
HOGENOMiHOG000151490.
InParanoidiO80526.
KOiK01648.
OMAiEKQMTPD.
OrthoDBiEOG09360AMI.
PhylomeDBiO80526.

Miscellaneous databases

PROiO80526.

Gene expression databases

GenevisibleiO80526. AT.

Family and domain databases

Gene3Di3.30.470.20. 1 hit.
3.40.50.261. 1 hit.
InterProiIPR013650. ATP-grasp_succ-CoA_synth-type.
IPR013816. ATP_grasp_subdomain_2.
IPR032263. Citrate-bd.
IPR016102. Succinyl-CoA_synth-like.
[Graphical view]
PfamiPF08442. ATP-grasp_2. 1 hit.
PF16114. Citrate_bind. 1 hit.
[Graphical view]
SUPFAMiSSF52210. SSF52210. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiACLA3_ARATH
AccessioniPrimary (citable) accession number: O80526
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: November 1, 1998
Last modified: November 30, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.