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Protein

Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase

Gene

At2g44660

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Adds the second glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc1Man9GlcNAc(2)-PP-Dol (By similarity).By similarity

Catalytic activityi

Dolichyl beta-D-glucosyl phosphate + D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Glc-alpha-(1->3)-D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciARA:AT2G44660-MONOMER.
ReactomeiR-ATH-446193. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT57. Glycosyltransferase Family 57.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase (EC:2.4.1.265)
Alternative name(s):
Asparagine-linked glycosylation protein 8 homolog
Dol-P-Glc:Glc(1)Man(9)GlcNAc(2)-PP-dolichyl alpha-1,3-glucosyltransferase
Dolichyl-P-Glc:Glc1Man9GlcNAc2-PP-dolichyl glucosyltransferase
Gene namesi
Ordered Locus Names:At2g44660
ORF Names:F16B22.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G44660.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei10 – 3021HelicalSequence analysisAdd
BLAST
Transmembranei101 – 12121HelicalSequence analysisAdd
BLAST
Transmembranei133 – 15321HelicalSequence analysisAdd
BLAST
Transmembranei176 – 19621HelicalSequence analysisAdd
BLAST
Transmembranei219 – 23921HelicalSequence analysisAdd
BLAST
Transmembranei261 – 28121HelicalSequence analysisAdd
BLAST
Transmembranei305 – 32521HelicalSequence analysisAdd
BLAST
Transmembranei339 – 35921HelicalSequence analysisAdd
BLAST
Transmembranei384 – 40118HelicalSequence analysisAdd
BLAST
Transmembranei406 – 42621HelicalSequence analysisAdd
BLAST
Transmembranei454 – 47421HelicalSequence analysisAdd
BLAST
Transmembranei479 – 49921HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 506506Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferasePRO_0000174166Add
BLAST

Proteomic databases

PaxDbiO80505.
PRIDEiO80505.

Expressioni

Gene expression databases

GenevisibleiO80505. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G44660.1.

Structurei

3D structure databases

ProteinModelPortaliO80505.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2576. Eukaryota.
ENOG410XPPM. LUCA.
HOGENOMiHOG000168174.
InParanoidiO80505.
KOiK03849.
OMAiWKLKYPF.
PhylomeDBiO80505.

Family and domain databases

InterProiIPR004856. Glyco_trans_ALG6/ALG8.
[Graphical view]
PANTHERiPTHR12413. PTHR12413. 1 hit.
PfamiPF03155. Alg6_Alg8. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O80505-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDHREKSDRR LLLWFFAVAT AVKLLLIPSS RSTDFEVHRN WLAITNSLPL
60 70 80 90 100
TKWYFDETSQ WTLDYPPFFA YFERFLSIFA RLVDPRIVDL QSGLDYNAES
110 120 130 140 150
VIYFQRISVI VSDLCLLYGV YRLTRKLEPL KRNLICALVI WSPGLLIVDH
160 170 180 190 200
IHFQYNGFLL GWLLLSISFL QEGRDLLGGF LFAVLLCFKH LFAVTAPVYF
210 220 230 240 250
VYLLRHYCWS GLVTGFRRLV TIGAVVVAVF AAAYGPFIYH GQIQQVISRM
260 270 280 290 300
FPFGRGLCHA YWAPNFWVFY IILDKGLAVL LRKLGYEIQI PSASFTGGLV
310 320 330 340 350
GDSSPFAVLP QITPLTTFAM VLLAISPCLI KAWKKPHSGL VARWVAYAYT
360 370 380 390 400
CGFLFGWHVH EKASLHFTIP LAIVAVQSLE DAKHYFLVSI VSCYSLYPLL
410 420 430 440 450
YEPQEYPIKV LLLLLHSVVM WLGFAAQYTD YKAQKKETSE IKSKFRIGCF
460 470 480 490 500
EKSYLMGLII VEIVSQFLHP YFLGDKLPFL PLMLISTYCT VGIMYSWIWQ

LRKILT
Length:506
Mass (Da):58,222
Last modified:February 8, 2011 - v3
Checksum:iC23639FAE29F1413
GO

Sequence cautioni

The sequence AAC27468.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti501 – 5011L → P in BX842470 (PubMed:14993207).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC003672 Genomic DNA. Translation: AAC27468.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC10452.1.
BX842470 mRNA. No translation available.
PIRiT01593.
RefSeqiNP_181994.5. NM_130030.5.
UniGeneiAt.25358.
At.53123.

Genome annotation databases

EnsemblPlantsiAT2G44660.1; AT2G44660.1; AT2G44660.
GeneIDi819074.
GrameneiAT2G44660.1; AT2G44660.1; AT2G44660.
KEGGiath:AT2G44660.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC003672 Genomic DNA. Translation: AAC27468.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC10452.1.
BX842470 mRNA. No translation available.
PIRiT01593.
RefSeqiNP_181994.5. NM_130030.5.
UniGeneiAt.25358.
At.53123.

3D structure databases

ProteinModelPortaliO80505.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G44660.1.

Protein family/group databases

CAZyiGT57. Glycosyltransferase Family 57.

Proteomic databases

PaxDbiO80505.
PRIDEiO80505.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G44660.1; AT2G44660.1; AT2G44660.
GeneIDi819074.
GrameneiAT2G44660.1; AT2G44660.1; AT2G44660.
KEGGiath:AT2G44660.

Organism-specific databases

TAIRiAT2G44660.

Phylogenomic databases

eggNOGiKOG2576. Eukaryota.
ENOG410XPPM. LUCA.
HOGENOMiHOG000168174.
InParanoidiO80505.
KOiK03849.
OMAiWKLKYPF.
PhylomeDBiO80505.

Enzyme and pathway databases

UniPathwayiUPA00378.
BioCyciARA:AT2G44660-MONOMER.
ReactomeiR-ATH-446193. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.

Miscellaneous databases

PROiO80505.

Gene expression databases

GenevisibleiO80505. AT.

Family and domain databases

InterProiIPR004856. Glyco_trans_ALG6/ALG8.
[Graphical view]
PANTHERiPTHR12413. PTHR12413. 1 hit.
PfamiPF03155. Alg6_Alg8. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation."
    Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M.
    Genome Res. 14:406-413(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 415-506.
    Strain: cv. Columbia.

Entry informationi

Entry nameiALG8_ARATH
AccessioniPrimary (citable) accession number: O80505
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: February 8, 2011
Last modified: February 17, 2016
This is version 105 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.