Reviewed, UniProtKB/Swiss-Prot O80505 (ALG8_ARATH)

Names and origin

Protein names

Recommended name:
    Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase
    EC=2.4.1.-
Alternative name(s):
    Dolichyl-P-Glc:Glc1Man9GlcNAc2-PP-dolichyl glucosyltransferase
    Asparagine-linked glycosylation protein 8

Gene names

Ordered Locus Names:	At2g44660
ORF Names:	F16B22.15

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	418 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Adds the second glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc1Man9GlcNAc(2)-PP-Dol By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the ALG6/ALG8 glucosyltransferase family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring glycosyl groups Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 418	418	Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase		PRO_0000174166
Regions
	Transmembrane	10 – 30	21	Potential
	Transmembrane	101 – 121	21	Potential
	Transmembrane	133 – 153	21	Potential
	Transmembrane	176 – 196	21	Potential
	Transmembrane	219 – 239	21	Potential
	Transmembrane	261 – 281	21	Potential
	Transmembrane	305 – 325	21	Potential
	Transmembrane	339 – 359	21	Potential
	Transmembrane	384 – 401	18	Potential

Sequences

	Sequence		Length	Mass (Da)
	O80505-1 [UniParc]. Last modified March 24, 2009. Version 2. Checksum: 8434FF9A2068155B	FASTA	418	47,975
	10 20 30 40 50 60 MDHREKSDRR LLLWFFAVAT AVKLLLIPSS RSTDFEVHRN WLAITNSLPL TKWYFDETSQ 70 80 90 100 110 120 WTLDYPPFFA YFERFLSIFA RLVDPRIVDL QSGLDYNAES VIYFQRISVI VSDLCLLYGV 130 140 150 160 170 180 YRLTRKLEPL KRNLICALVI WSPGLLIVDH IHFQYNGFLL GWLLLSISFL QEGRDLLGGF 190 200 210 220 230 240 LFAVLLCFKH LFAVTAPVYF VYLLRHYCWS GLVTGFRRLV TIGAVVVAVF AAAYGPFIYH 250 260 270 280 290 300 GQIQQVISRM FPFGRGLCHA YWAPNFWVFY IILDKGLAVL LRKLGYEIQI PSASFTGGLV 310 320 330 340 350 360 GDSSPFAVLP QITPLTTFAM VLLAISPCLI KAWKKPHSGL VARWVAYAYT CGFLFGWHVH 370 380 390 400 410 EKASLHFTIP LAIVAVQSLE DAKHYFLVSI VSCYSLYPLL YEPRSTRSRF CCCYCIPW

References

[1]

"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC003672 Genomic DNA. Translation: AAC27468.1.
IPI	IPI00548931.
PIR	T01593.
RefSeq	NP_181994.2.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT57. Glycosyltransferase Family 57.
Proteomic databases
PRIDE	O80505.
Genome annotation databases
GeneID	819074.
GenomeReviews	Gene locus AT2G44660 in contig CT485783_GR.
NMPDR	fig|3702.1.peg.11644.
Organism-specific databases
TAIR	At2g44660.
Gene expression databases
ArrayExpress	O80505.
GermOnline	AT2G44660. Arabidopsis thaliana.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

ALG8_ARATH

Accession

Primary (citable) accession number: O80505

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	March 24, 2009
Last modified:	June 16, 2009
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)