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Protein

Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase

Gene

At2g44660

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Adds the second glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc1Man9GlcNAc2-PP-Dol (By similarity).By similarity

Catalytic activityi

Dolichyl beta-D-glucosyl phosphate + D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Glc-alpha-(1->3)-D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciARA:AT2G44660-MONOMER
ReactomeiR-ATH-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
UniPathwayiUPA00378

Protein family/group databases

CAZyiGT57 Glycosyltransferase Family 57

Names & Taxonomyi

Protein namesi
Recommended name:
Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase (EC:2.4.1.265)
Alternative name(s):
Asparagine-linked glycosylation protein 8 homolog
Dol-P-Glc:Glc(1)Man(9)GlcNAc(2)-PP-dolichyl alpha-1,3-glucosyltransferase
Dolichyl-P-Glc:Glc1Man9GlcNAc2-PP-dolichyl glucosyltransferase
Gene namesi
Ordered Locus Names:At2g44660
ORF Names:F16B22.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G44660
TAIRilocus:2042411 AT2G44660

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei10 – 30HelicalSequence analysisAdd BLAST21
Transmembranei101 – 121HelicalSequence analysisAdd BLAST21
Transmembranei133 – 153HelicalSequence analysisAdd BLAST21
Transmembranei176 – 196HelicalSequence analysisAdd BLAST21
Transmembranei219 – 239HelicalSequence analysisAdd BLAST21
Transmembranei261 – 281HelicalSequence analysisAdd BLAST21
Transmembranei305 – 325HelicalSequence analysisAdd BLAST21
Transmembranei339 – 359HelicalSequence analysisAdd BLAST21
Transmembranei384 – 401HelicalSequence analysisAdd BLAST18
Transmembranei406 – 426HelicalSequence analysisAdd BLAST21
Transmembranei454 – 474HelicalSequence analysisAdd BLAST21
Transmembranei479 – 499HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001741661 – 506Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferaseAdd BLAST506

Proteomic databases

PaxDbiO80505
PRIDEiO80505

Expressioni

Gene expression databases

ExpressionAtlasiO80505 baseline and differential
GenevisibleiO80505 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G44660.1

Structurei

3D structure databases

ProteinModelPortaliO80505
SMRiO80505
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2576 Eukaryota
ENOG410XPPM LUCA
HOGENOMiHOG000168174
InParanoidiO80505
KOiK03849
OMAiALTLCGY
OrthoDBiEOG09360BJO
PhylomeDBiO80505

Family and domain databases

InterProiView protein in InterPro
IPR004856 Glyco_trans_ALG6/ALG8
PANTHERiPTHR12413 PTHR12413, 1 hit
PfamiView protein in Pfam
PF03155 Alg6_Alg8, 1 hit

Sequencei

Sequence statusi: Complete.

O80505-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDHREKSDRR LLLWFFAVAT AVKLLLIPSS RSTDFEVHRN WLAITNSLPL
60 70 80 90 100
TKWYFDETSQ WTLDYPPFFA YFERFLSIFA RLVDPRIVDL QSGLDYNAES
110 120 130 140 150
VIYFQRISVI VSDLCLLYGV YRLTRKLEPL KRNLICALVI WSPGLLIVDH
160 170 180 190 200
IHFQYNGFLL GWLLLSISFL QEGRDLLGGF LFAVLLCFKH LFAVTAPVYF
210 220 230 240 250
VYLLRHYCWS GLVTGFRRLV TIGAVVVAVF AAAYGPFIYH GQIQQVISRM
260 270 280 290 300
FPFGRGLCHA YWAPNFWVFY IILDKGLAVL LRKLGYEIQI PSASFTGGLV
310 320 330 340 350
GDSSPFAVLP QITPLTTFAM VLLAISPCLI KAWKKPHSGL VARWVAYAYT
360 370 380 390 400
CGFLFGWHVH EKASLHFTIP LAIVAVQSLE DAKHYFLVSI VSCYSLYPLL
410 420 430 440 450
YEPQEYPIKV LLLLLHSVVM WLGFAAQYTD YKAQKKETSE IKSKFRIGCF
460 470 480 490 500
EKSYLMGLII VEIVSQFLHP YFLGDKLPFL PLMLISTYCT VGIMYSWIWQ

LRKILT
Length:506
Mass (Da):58,222
Last modified:February 8, 2011 - v3
Checksum:iC23639FAE29F1413
GO

Sequence cautioni

The sequence AAC27468 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti501L → P in BX842470 (PubMed:14993207).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC003672 Genomic DNA Translation: AAC27468.1 Sequence problems.
CP002685 Genomic DNA Translation: AEC10452.1
BX842470 mRNA No translation available.
PIRiT01593
RefSeqiNP_181994.5, NM_130030.5
UniGeneiAt.25358
At.53123

Genome annotation databases

EnsemblPlantsiAT2G44660.1; AT2G44660.1; AT2G44660
GeneIDi819074
GrameneiAT2G44660.1; AT2G44660.1; AT2G44660
KEGGiath:AT2G44660

Similar proteinsi

Entry informationi

Entry nameiALG8_ARATH
AccessioniPrimary (citable) accession number: O80505
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: February 8, 2011
Last modified: May 23, 2018
This is version 116 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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