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O80505 (ALG8_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase
EC=2.4.1.265
Alternative name(s):
Asparagine-linked glycosylation protein 8 homolog
Dol-P-Glc:Glc(1)Man(9)GlcNAc(2)-PP-dolichyl alpha-1,3-glucosyltransferase
Dolichyl-P-Glc:Glc1Man9GlcNAc2-PP-dolichyl glucosyltransferase
Gene names
Ordered Locus Names:At2g44660
ORF Names:F16B22.15
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length506 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Adds the second glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc1Man9GlcNAc(2)-PP-Dol By similarity.

Catalytic activity

Dolichyl beta-D-glucosyl phosphate + D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Glc-alpha-(1->3)-D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.

Sequence similarities

Belongs to the ALG6/ALG8 glucosyltransferase family.

Sequence caution

The sequence AAC27468.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 506506Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase
PRO_0000174166

Regions

Transmembrane10 – 3021Helical; Potential
Transmembrane101 – 12121Helical; Potential
Transmembrane133 – 15321Helical; Potential
Transmembrane176 – 19621Helical; Potential
Transmembrane219 – 23921Helical; Potential
Transmembrane261 – 28121Helical; Potential
Transmembrane305 – 32521Helical; Potential
Transmembrane339 – 35921Helical; Potential
Transmembrane384 – 40118Helical; Potential
Transmembrane406 – 42621Helical; Potential
Transmembrane454 – 47421Helical; Potential
Transmembrane479 – 49921Helical; Potential

Experimental info

Sequence conflict5011L → P in BX842470. Ref.3

Sequences

Sequence LengthMass (Da)Tools
O80505 [UniParc].

Last modified February 8, 2011. Version 3.
Checksum: C23639FAE29F1413

FASTA50658,222
        10         20         30         40         50         60 
MDHREKSDRR LLLWFFAVAT AVKLLLIPSS RSTDFEVHRN WLAITNSLPL TKWYFDETSQ 

        70         80         90        100        110        120 
WTLDYPPFFA YFERFLSIFA RLVDPRIVDL QSGLDYNAES VIYFQRISVI VSDLCLLYGV 

       130        140        150        160        170        180 
YRLTRKLEPL KRNLICALVI WSPGLLIVDH IHFQYNGFLL GWLLLSISFL QEGRDLLGGF 

       190        200        210        220        230        240 
LFAVLLCFKH LFAVTAPVYF VYLLRHYCWS GLVTGFRRLV TIGAVVVAVF AAAYGPFIYH 

       250        260        270        280        290        300 
GQIQQVISRM FPFGRGLCHA YWAPNFWVFY IILDKGLAVL LRKLGYEIQI PSASFTGGLV 

       310        320        330        340        350        360 
GDSSPFAVLP QITPLTTFAM VLLAISPCLI KAWKKPHSGL VARWVAYAYT CGFLFGWHVH 

       370        380        390        400        410        420 
EKASLHFTIP LAIVAVQSLE DAKHYFLVSI VSCYSLYPLL YEPQEYPIKV LLLLLHSVVM 

       430        440        450        460        470        480 
WLGFAAQYTD YKAQKKETSE IKSKFRIGCF EKSYLMGLII VEIVSQFLHP YFLGDKLPFL 

       490        500 
PLMLISTYCT VGIMYSWIWQ LRKILT

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed: 10617197] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation."
Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M.
Genome Res. 14:406-413(2004) [PubMed: 14993207] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 415-506.
Strain: cv. Columbia.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AC003672 Genomic DNA. Translation: AAC27468.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC10452.1.
BX842470 mRNA. No translation available.
IPIIPI00548931.
PIRT01593.
RefSeqNP_181994.5. NM_130030.5.
UniGeneAt.25358.
At.53123.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGO80505.

Protein family/group databases

CAZyGT57. Glycosyltransferase Family 57.

Proteomic databases

PRIDEO80505.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G44660.1; AT2G44660.1; AT2G44660.
GeneID819074.
GenomeReviewsGene locus AT2G44660 in contig CT485783_GR.
KEGGath:AT2G44660.
NMPDRfig|3702.1.peg.11644.

Organism-specific databases

TAIRAt2g44660.

Phylogenomic databases

eggNOGKOG2576.
GeneTreeEPGT00070000030168.
HOGENOMHBG560959.
PhylomeDBO80505.

Gene expression databases

ArrayExpressO80505.
GenevestigatorO80505.
GermOnlineAT2G44660. Arabidopsis thaliana.

Family and domain databases

InterProIPR004856. Glyco_trans_ALG6/ALG8.
[Graphical view]
KOK03849.
PANTHERPTHR12413. Alg6_Alg8. 1 hit.
PfamPF03155. Alg6_Alg8. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameALG8_ARATH
AccessionPrimary (citable) accession number: O80505
Entry history
Integrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: February 8, 2011
Last modified: December 14, 2011
This is version 79 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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