Reviewed, UniProtKB/Swiss-Prot O80497 (GUN15_ARATH)

Names and origin

Protein names

Recommended name:
    Endoglucanase 15
    EC=3.2.1.4
Alternative name(s):
    Endo-1,4-beta glucanase 15

Gene names

Ordered Locus Names:	At2g44570
ORF Names:	F16B22.6

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	492 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 9 (cellulase E) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cell wall biogenesis/degradation Cellulose degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30	Potential
	Chain	31 – 492	462	Endoglucanase 15		PRO_0000249267
Sites
	Active site	414	1	By similarity
	Active site	466	1	By similarity
	Active site	475	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O80497-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 6FE816C89048D620	FASTA	492	53,591
	10 20 30 40 50 60 MSCISSQCFI TIKSICIVLL LSITCGAVSA NSNYGEALTK SLLYFEAQRS GKLPLDQRVI 70 80 90 100 110 120 WRGDSALRDG SDAHVDLTGG YYDAGDNMKF GFPLAFTTTM LAWSSVEMES QLKAHQEHGN 130 140 150 160 170 180 TLAALRWATD YLIKAHPEPN VLYGQVGDAN LDHACWMRPE DMTTPRPSYR IDPQHPGADL 190 200 210 220 230 240 AGETAAAMAA ASLAFAPSDS AYAKTLISHA KELFEFAKDY PGVYHNSIPN AGGFYPSSGY 250 260 270 280 290 300 EDELLWAAAW LHRATGDQTY LDHLTQASNS GGARSVFAWD DKFLGAQVLV AKLVFEGKVK 310 320 330 340 350 360 NEGKMIEYKS MAEQFICNCA QKGFNNVKKT PGGLLWFLPW DNLQYTATAS FALATYAKYL 370 380 390 400 410 420 EAAQTSIQCP NGDVLQASDL LNLARAQVDY ILGSNPKKMS YMVGYGTNYP KRPHHRGASI 430 440 450 460 470 480 VSIKNDPKPV TCNGGFEAWY NNPKPNPNLL VGAIVGGPDE YDAYGDERSD FQHGEPDTVT 490 VAPLLGVLAA IA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene family." Libertini E., Li Y., McQueen-Mason S.J. J. Mol. Evol. 58:506-515(2004) [PubMed: 15170254] [Abstract] Cited for: GENE FAMILY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC003672 Genomic DNA. Translation: AAC27459.1.
IPI	IPI00538004.
PIR	T01584.
RefSeq	NP_181985.1.
UniGene	At.36741
3D structure databases
HSSP	HSSP built from PDB template 1KS8 based on UniProtKB O77044.
ModBase	Search...
Protein family/group databases
CAZy	GH9. Glycoside Hydrolase Family 9.
Genome annotation databases
GeneID	819065.
GenomeReviews	Gene locus AT2G44570 in contig CT485783_GR.
KEGG	ath:AT2G44570.
NMPDR	fig|3702.1.peg.11633.
Organism-specific databases
TAIR	At2g44570.
Enzyme and pathway databases
BRENDA	3.2.1.4. 302.
Gene expression databases
ArrayExpress	O80497.
Genevestigator	O80497.
GermOnline	AT2G44570. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR012341. 6hp_glycosidase. IPR001701. Glyco_hydro_9. IPR018221. Glyco_hydro_9_AS. [Graphical view]
Gene3D	G3DSA:1.50.10.10. CelA/Cel48F_cat. 1 hit.
PANTHER	PTHR22298:SF3. Glyco_hydro_9. 1 hit.
Pfam	PF00759. Glyco_hydro_9. 1 hit. [Graphical view]
PROSITE	PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit. PS00698. GLYCOSYL_HYDROL_F9_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN15_ARATH

Accession

Primary (citable) accession number: O80497

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	November 1, 1998
Last modified:	October 13, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)