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Protein

Extra-large guanine nucleotide-binding protein 1

Gene

XLG1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems (By similarity). Binds GTP with specificity. Plays a role in the root morphogenesis by regulation of the cell proliferation.By similarity1 Publication

Miscellaneous

Dark-grown xlg1-1 xlg2-1 xlg3-1 triple mutant plants showed markedly increased primary root length compared with wild-type plants. Dark-grown roots of the xlg triple mutants also showed altered sensitivity to sugars, abscisic acid (ABA) hyposensitivity and ethylene hypersensitivity, whereas seed germination in xlg triple mutants was hypersensitive to osmotic stress and ABA (PubMed:17999646).1 Publication

Cofactori

Ca2+1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi497CalciumSequence analysis1
Metal bindingi669CalciumSequence analysis1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri225 – 268RING-type; degenerateAdd BLAST44
Nucleotide bindingi490 – 498GTPBy similarity9
Nucleotide bindingi661 – 669GTPBy similarity9
Nucleotide bindingi706 – 710GTP; degenerateBy similarity5
Nucleotide bindingi774 – 777GTPBy similarity4

GO - Molecular functioni

  • G-protein beta/gamma-subunit complex binding Source: GO_Central
  • G-protein coupled receptor binding Source: GO_Central
  • GTPase activity Source: UniProtKB
  • GTP binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • signal transducer activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionTransducer
LigandCalcium, GTP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiR-ATH-112043 PLC beta mediated events
R-ATH-202040 G-protein activation
R-ATH-2514859 Inactivation, recovery and regulation of the phototransduction cascade
R-ATH-399997 Acetylcholine regulates insulin secretion
R-ATH-416476 G alpha (q) signalling events
R-ATH-416482 G alpha (12/13) signalling events
R-ATH-418555 G alpha (s) signalling events
R-ATH-434316 Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion

Names & Taxonomyi

Protein namesi
Recommended name:
Extra-large guanine nucleotide-binding protein 1
Alternative name(s):
Extra-large GTP-binding protein 1
Short name:
Extra-large G-protein 1
Gene namesi
Name:XLG1
Synonyms:XLG
Ordered Locus Names:At2g23460
ORF Names:F26B6.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G23460
TAIRilocus:2046738 AT2G23460

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi497S → N: Strongly reduces GTP-binding and GTPase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004233971 – 888Extra-large guanine nucleotide-binding protein 1Add BLAST888

Proteomic databases

PaxDbiO80462
PRIDEiO80462

Expressioni

Tissue specificityi

Ubiquitous. Strongly expressed in vascular tissues, root and shoot meristems and lateral root primordia.2 Publications

Gene expression databases

ExpressionAtlasiO80462 baseline and differential
GenevisibleiO80462 AT

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi3702.AT2G23460.1

Structurei

3D structure databases

ProteinModelPortaliO80462
SMRiO80462
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi90 – 97TonB box8
Motifi205 – 222Nuclear localization signalSequence analysisAdd BLAST18

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi104 – 110Poly-Glu7
Compositional biasi196 – 199Poly-Glu4

Domaini

The helical domain (514-664) is required for self-activation.By similarity

Sequence similaritiesi

Belongs to the G-alpha family. XLG subfamily.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri225 – 268RING-type; degenerateAdd BLAST44

Keywords - Domaini

TonB box, Zinc-finger

Phylogenomic databases

eggNOGiKOG0082 Eukaryota
ENOG410XNVQ LUCA
HOGENOMiHOG000241648
InParanoidiO80462
OMAiKLCEANQ
OrthoDBiEOG093602MX
PhylomeDBiO80462

Family and domain databases

CDDicd00066 G-alpha, 1 hit
Gene3Di1.10.400.10, 3 hits
InterProiView protein in InterPro
IPR001019 Gprotein_alpha_su
IPR011025 GproteinA_insert
IPR027417 P-loop_NTPase
PANTHERiPTHR10218 PTHR10218, 1 hit
PfamiView protein in Pfam
PF00503 G-alpha, 1 hit
PRINTSiPR00318 GPROTEINA
SMARTiView protein in SMART
SM00275 G_alpha, 1 hit
SUPFAMiSSF47895 SSF47895, 1 hit
SSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS00430 TONB_DEPENDENT_REC_1, 1 hit

Sequencei

Sequence statusi: Complete.

O80462-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLKEDDCCL FAEEYDGPPL SYNIPCAVPI NVEKIPVAAV VSPVCISDNM
60 70 80 90 100
SFPVIQPILS VESKKFLIDS VSPTSVIANC GSNQLELVSD SITVSPTSVI
110 120 130 140 150
EHTEEEEEEE GGDGEDCELS SSGELLLRSC SVKESLDLNE SSSNPLVPDW
160 170 180 190 200
ESNESVLSMD YPSSRVTGDC VSETNGDGKK QPVVTFLGIA SDDGFEEEES
210 220 230 240 250
CSNLRRVRVV PVKKQPQTKG KKGSCYRCFK GSRFTEKEVC LVCDAKYCNS
260 270 280 290 300
CVLRAMGSMP EGRKCVTCIG FPIDESKRGS LGKCSRMLKR LLNDLEVKQI
310 320 330 340 350
MKTERFCEAN QLPAEYVYVN GQPLYPEELV TLQTCSNPPK KLKPGDYWYD
360 370 380 390 400
KVSGLWGKEG EKPYQIISPH LNVGGPISPE ASNGNTQVFI NGREITKVEL
410 420 430 440 450
RMLQLAGVQC AGNPHFWVNE DGSYQEEGQK NTKGYIWGKA GTKLLCAVLS
460 470 480 490 500
LPVPSKSTAN ASGEQLYSAN SRSILDHLEH RTLQKILLVG NSGSGTSTIF
510 520 530 540 550
KQAKILYKDV PFLEDERENI KVIIQTNVYG YLGMLLEGRE RFEEEALALR
560 570 580 590 600
NTKQCVLENI PADEGDAKSN DKTVTMYSIG PRLKAFSDWL LKTMAAGNLG
610 620 630 640 650
VIFPAASREY APLVEELWRD AAIQATYKRR SELGLLPSVA SYFLERAIDV
660 670 680 690 700
LTPDYEPSDL DILYAEGVTS SSGLACLDFS FPQTASEENL DPSDHHDSLL
710 720 730 740 750
RYQLIRVPSR GLGENCKWID MFEDVGMVVF VVSMSDYDQV SEDGTNKMLL
760 770 780 790 800
TKKLFESIIT HPIFENMDFL LILNKYDLLE EKVERVPLAR CEWFQDFNPV
810 820 830 840 850
VSRHRGSNNG NPTLGQLAFH FMAVKFKRFY SSLTGKKLFV SSSKSLDPNS
860 870 880
VDSSLKLAME ILKWSEERTN ICMSEYSMYS TEPSSFSN
Length:888
Mass (Da):98,796
Last modified:June 1, 2002 - v2
Checksum:i61F57223CE663648
GO

Sequence cautioni

The sequence AAK96856 differs from that shown. Reason: Erroneous termination at position 780. Translated as Glu.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti45C → R in AAC19353 (PubMed:10394945).Curated1
Sequence conflicti204L → Q in AAC19353 (PubMed:10394945).Curated1
Sequence conflicti249N → S in AAC19353 (PubMed:10394945).Curated1
Sequence conflicti336S → P in AAC19353 (PubMed:10394945).Curated1
Sequence conflicti641S → N in AAC19353 (PubMed:10394945).Curated1
Sequence conflicti660L → V in AAC19353 (PubMed:10394945).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF060941 mRNA Translation: AAC19352.1
AF060942 Genomic DNA Translation: AAC19353.1
AC003040 Genomic DNA Translation: AAC23761.2
CP002685 Genomic DNA Translation: AEC07458.1
AY054665 mRNA Translation: AAK96856.1 Different termination.
PIRiT01135
T51593
RefSeqiNP_565553.1, NM_127910.3
UniGeneiAt.48510
At.66350

Genome annotation databases

EnsemblPlantsiAT2G23460.1; AT2G23460.1; AT2G23460
GeneIDi816878
GrameneiAT2G23460.1; AT2G23460.1; AT2G23460
KEGGiath:AT2G23460

Similar proteinsi

Entry informationi

Entry nameiXLG1_ARATH
AccessioniPrimary (citable) accession number: O80462
Secondary accession number(s): O81224, O81225, Q93Y21
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 18, 2013
Last sequence update: June 1, 2002
Last modified: May 23, 2018
This is version 132 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families
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