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Protein

Extra-large guanine nucleotide-binding protein 1

Gene

XLG1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems (By similarity). Binds GTP with specificity. Plays a role in the root morphogenesis by regulation of the cell proliferation.By similarity1 Publication

Cofactori

Ca2+1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi497 – 4971CalciumSequence analysis
Metal bindingi669 – 6691CalciumSequence analysis

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri225 – 26844RING-type; degenerateAdd
BLAST
Nucleotide bindingi490 – 4989GTPBy similarity
Nucleotide bindingi661 – 6699GTPBy similarity
Nucleotide bindingi706 – 7105GTP; degenerateBy similarity
Nucleotide bindingi774 – 7774GTPBy similarity

GO - Molecular functioni

  • GTPase activity Source: UniProtKB
  • GTP binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • signal transducer activity Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transducer

Keywords - Ligandi

Calcium, GTP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiR-ATH-399997. Acetylcholine regulates insulin secretion.
R-ATH-416476. G alpha (q) signalling events.
R-ATH-416482. G alpha (12/13) signalling events.
R-ATH-418555. G alpha (s) signalling events.
R-ATH-434316. Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion.

Names & Taxonomyi

Protein namesi
Recommended name:
Extra-large guanine nucleotide-binding protein 1
Alternative name(s):
Extra-large GTP-binding protein 1
Short name:
Extra-large G-protein 1
Gene namesi
Name:XLG1
Synonyms:XLG
Ordered Locus Names:At2g23460
ORF Names:F26B6.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G23460.

Subcellular locationi

  • Nucleus 1 Publication

GO - Cellular componenti

  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi497 – 4971S → N: Strongly reduces GTP-binding and GTPase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 888888Extra-large guanine nucleotide-binding protein 1PRO_0000423397Add
BLAST

Proteomic databases

PaxDbiO80462.
PRIDEiO80462.

Expressioni

Tissue specificityi

Ubiquitous. Strongly expressed in vascular tissues, root and shoot meristems and lateral root primordia.2 Publications

Gene expression databases

GenevisibleiO80462. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G23460.1.

Structurei

3D structure databases

ProteinModelPortaliO80462.
SMRiO80462. Positions 477-863.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi90 – 978TonB box
Motifi205 – 22218Nuclear localization signalSequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi104 – 1107Poly-Glu
Compositional biasi196 – 1994Poly-Glu

Domaini

The helical domain (514-664) is required for self-activation.By similarity

Sequence similaritiesi

Belongs to the G-alpha family. XLG subfamily.Curated
Contains 1 RING-type zinc finger.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri225 – 26844RING-type; degenerateAdd
BLAST

Keywords - Domaini

TonB box, Zinc-finger

Phylogenomic databases

eggNOGiKOG0082. Eukaryota.
ENOG410XNVQ. LUCA.
HOGENOMiHOG000241648.
InParanoidiO80462.
OMAiIQSNIYN.
OrthoDBiEOG093602MX.
PhylomeDBiO80462.

Family and domain databases

Gene3Di1.10.400.10. 2 hits.
3.40.50.300. 2 hits.
InterProiIPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10218. PTHR10218. 4 hits.
PfamiPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSiPR00318. GPROTEINA.
SMARTiSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS00430. TONB_DEPENDENT_REC_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O80462-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLKEDDCCL FAEEYDGPPL SYNIPCAVPI NVEKIPVAAV VSPVCISDNM
60 70 80 90 100
SFPVIQPILS VESKKFLIDS VSPTSVIANC GSNQLELVSD SITVSPTSVI
110 120 130 140 150
EHTEEEEEEE GGDGEDCELS SSGELLLRSC SVKESLDLNE SSSNPLVPDW
160 170 180 190 200
ESNESVLSMD YPSSRVTGDC VSETNGDGKK QPVVTFLGIA SDDGFEEEES
210 220 230 240 250
CSNLRRVRVV PVKKQPQTKG KKGSCYRCFK GSRFTEKEVC LVCDAKYCNS
260 270 280 290 300
CVLRAMGSMP EGRKCVTCIG FPIDESKRGS LGKCSRMLKR LLNDLEVKQI
310 320 330 340 350
MKTERFCEAN QLPAEYVYVN GQPLYPEELV TLQTCSNPPK KLKPGDYWYD
360 370 380 390 400
KVSGLWGKEG EKPYQIISPH LNVGGPISPE ASNGNTQVFI NGREITKVEL
410 420 430 440 450
RMLQLAGVQC AGNPHFWVNE DGSYQEEGQK NTKGYIWGKA GTKLLCAVLS
460 470 480 490 500
LPVPSKSTAN ASGEQLYSAN SRSILDHLEH RTLQKILLVG NSGSGTSTIF
510 520 530 540 550
KQAKILYKDV PFLEDERENI KVIIQTNVYG YLGMLLEGRE RFEEEALALR
560 570 580 590 600
NTKQCVLENI PADEGDAKSN DKTVTMYSIG PRLKAFSDWL LKTMAAGNLG
610 620 630 640 650
VIFPAASREY APLVEELWRD AAIQATYKRR SELGLLPSVA SYFLERAIDV
660 670 680 690 700
LTPDYEPSDL DILYAEGVTS SSGLACLDFS FPQTASEENL DPSDHHDSLL
710 720 730 740 750
RYQLIRVPSR GLGENCKWID MFEDVGMVVF VVSMSDYDQV SEDGTNKMLL
760 770 780 790 800
TKKLFESIIT HPIFENMDFL LILNKYDLLE EKVERVPLAR CEWFQDFNPV
810 820 830 840 850
VSRHRGSNNG NPTLGQLAFH FMAVKFKRFY SSLTGKKLFV SSSKSLDPNS
860 870 880
VDSSLKLAME ILKWSEERTN ICMSEYSMYS TEPSSFSN
Length:888
Mass (Da):98,796
Last modified:June 1, 2002 - v2
Checksum:i61F57223CE663648
GO

Sequence cautioni

The sequence AAK96856 differs from that shown. Reason: Erroneous termination at position 780. Translated as Glu.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti45 – 451C → R in AAC19353 (PubMed:10394945).Curated
Sequence conflicti204 – 2041L → Q in AAC19353 (PubMed:10394945).Curated
Sequence conflicti249 – 2491N → S in AAC19353 (PubMed:10394945).Curated
Sequence conflicti336 – 3361S → P in AAC19353 (PubMed:10394945).Curated
Sequence conflicti641 – 6411S → N in AAC19353 (PubMed:10394945).Curated
Sequence conflicti660 – 6601L → V in AAC19353 (PubMed:10394945).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF060941 mRNA. Translation: AAC19352.1.
AF060942 Genomic DNA. Translation: AAC19353.1.
AC003040 Genomic DNA. Translation: AAC23761.2.
CP002685 Genomic DNA. Translation: AEC07458.1.
AY054665 mRNA. Translation: AAK96856.1. Different termination.
PIRiT01135.
T51593.
RefSeqiNP_565553.1. NM_127910.2.
UniGeneiAt.48510.
At.66350.

Genome annotation databases

EnsemblPlantsiAT2G23460.1; AT2G23460.1; AT2G23460.
GeneIDi816878.
GrameneiAT2G23460.1; AT2G23460.1; AT2G23460.
KEGGiath:AT2G23460.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF060941 mRNA. Translation: AAC19352.1.
AF060942 Genomic DNA. Translation: AAC19353.1.
AC003040 Genomic DNA. Translation: AAC23761.2.
CP002685 Genomic DNA. Translation: AEC07458.1.
AY054665 mRNA. Translation: AAK96856.1. Different termination.
PIRiT01135.
T51593.
RefSeqiNP_565553.1. NM_127910.2.
UniGeneiAt.48510.
At.66350.

3D structure databases

ProteinModelPortaliO80462.
SMRiO80462. Positions 477-863.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G23460.1.

Proteomic databases

PaxDbiO80462.
PRIDEiO80462.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G23460.1; AT2G23460.1; AT2G23460.
GeneIDi816878.
GrameneiAT2G23460.1; AT2G23460.1; AT2G23460.
KEGGiath:AT2G23460.

Organism-specific databases

TAIRiAT2G23460.

Phylogenomic databases

eggNOGiKOG0082. Eukaryota.
ENOG410XNVQ. LUCA.
HOGENOMiHOG000241648.
InParanoidiO80462.
OMAiIQSNIYN.
OrthoDBiEOG093602MX.
PhylomeDBiO80462.

Enzyme and pathway databases

ReactomeiR-ATH-399997. Acetylcholine regulates insulin secretion.
R-ATH-416476. G alpha (q) signalling events.
R-ATH-416482. G alpha (12/13) signalling events.
R-ATH-418555. G alpha (s) signalling events.
R-ATH-434316. Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion.

Miscellaneous databases

PROiO80462.

Gene expression databases

GenevisibleiO80462. AT.

Family and domain databases

Gene3Di1.10.400.10. 2 hits.
3.40.50.300. 2 hits.
InterProiIPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10218. PTHR10218. 4 hits.
PfamiPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSiPR00318. GPROTEINA.
SMARTiSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS00430. TONB_DEPENDENT_REC_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiXLG1_ARATH
AccessioniPrimary (citable) accession number: O80462
Secondary accession number(s): O81224, O81225, Q93Y21
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 18, 2013
Last sequence update: June 1, 2002
Last modified: September 7, 2016
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Dark-grown xlg1-1 xlg2-1 xlg3-1 triple mutant plants showed markedly increased primary root length compared with wild-type plants. Dark-grown roots of the xlg triple mutants also showed altered sensitivity to sugars, abscisic acid (ABA) hyposensitivity and ethylene hypersensitivity, whereas seed germination in xlg triple mutants was hypersensitive to osmotic stress and ABA (PubMed:17999646).1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.