Reviewed, UniProtKB/Swiss-Prot O80437 (GPAT6_ARATH)

Names and origin

Protein names

Recommended name:
    Glycerol-3-phosphate acyltransferase 6
      Short name=AtGPAT6
    EC=2.3.1.15

Gene names

Name:	GPAT6
Ordered Locus Names:	At2g38110
ORF Names:	F16M14.4

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	501 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis.
Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate. Ref.3
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Tissue specificity	Specifically expressed in flower buds. Ref.3
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity.
Sequence similarities	Belongs to the GPAT/DAPAT family.
Biophysicochemical properties	Kinetic parameters: Vmax=278.56 pmol/min/mg enzyme

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Membrane
Domain	Transmembrane
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	1-acylglycerol-3-phosphate O-acyltransferase activity Ref.3 Inferred from genetic interaction. Source: TAIR glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 501	501	Glycerol-3-phosphate acyltransferase 6		PRO_0000195254
Regions
	Transmembrane	44 – 64	21	Potential
	Transmembrane	66 – 86	21	Potential
	Transmembrane	246 – 266	21	Potential
	Motif	313 – 318	6	HXXXXD motif

Sequences

	Sequence		Length	Mass (Da)
	O80437-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 72AD71555A6C7C60	FASTA	501	56,139
	10 20 30 40 50 60 MGAQEKRRRF EQISKCDVKD RSNHTVAADL DGTLLISRSA FPYYFLVALE AGSLLRALIL 70 80 90 100 110 120 LVSVPFVYLT YLTISETLAI NVFVFITFAG LKIRDVELVV RSVLPRFYAE DVRPDTWRIF 130 140 150 160 170 180 NTFGKRYIIT ASPRIMVEPF VKTFLGVDKV LGTELEVSKS GRATGFTRKP GILVGQYKRD 190 200 210 220 230 240 VVLREFGGLA SDLPDLGLGD SKTDHDFMSI CKEGYMVPRT KCEPLPRNKL LSPIIFHEGR 250 260 270 280 290 300 LVQRPTPLVA LLTFLWLPVG FVLSIIRVYT NIPLPERIAR YNYKLTGIKL VVNGHPPPPP 310 320 330 340 350 360 KPGQPGHLLV CNHRTVLDPV VTAVALGRKI SCVTYSISKF SELISPIKAV ALTRQREKDA 370 380 390 400 410 420 ANIKRLLEEG DLVICPEGTT CREPFLLRFS ALFAELTDRI VPVAINTKQS MFNGTTTRGY 430 440 450 460 470 480 KLLDPYFAFM NPRPTYEITF LKQIPAELTC KGGKSPIEVA NYIQRVLGGT LGFECTNFTR 490 500 KDKYAMLAGT DGRVPVKKEK T

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"Arabidopsis AtGPAT1, a member of the membrane-bound glycerol-3-phosphate acyltransferase gene family, is essential for tapetum differentiation and male fertility." Zheng Z., Xia Q., Dauk M., Shen W., Selvaraj G., Zou J. Plant Cell 15:1872-1887(2003) [PubMed: 12897259] [Abstract] Cited for: ENZYME ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC003028 Genomic DNA. Translation: AAC27160.1. AY062721 mRNA. Translation: AAL32799.1. AY114657 mRNA. Translation: AAM47976.1.
IPI	IPI00533394.
PIR	T01243.
RefSeq	NP_181346.1.
UniGene	At.21177 Rra.22748
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	O80437.
Genome annotation databases
GeneID	818389.
GenomeReviews	Gene locus AT2G38110 in contig CT485783_GR.
KEGG	ath:AT2G38110.
NMPDR	fig|3702.1.peg.10918.
Organism-specific databases
TAIR	At2g38110.
Phylogenomic databases
HOGENOM	HBG590278.
InParanoid	O80437.
OMA	KRYIITA.
Enzyme and pathway databases
BRENDA	2.3.1.15. 302.
Gene expression databases
ArrayExpress	O80437.
Genevestigator	O80437.
GermOnline	AT2G38110. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR002123. Acyltransferase. [Graphical view]
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GPAT6_ARATH

Accession

Primary (citable) accession number: O80437

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	November 1, 1998
Last modified:	February 9, 2010
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)