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Reviewed, UniProtKB/Swiss-Prot O80434 (LAC4_ARATH)

Last modified February 9, 2010. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Laccase-4
    EC=1.10.3.2
Alternative name(s):
    Benzenediol:oxygen oxidoreductase 4
    Urishiol oxidase 4
    Diphenol oxidase 4
    Protein IRREGULAR XYLEM 12
Gene names
Name: IRX12
Synonyms: LAC4
Ordered Locus Names: At2g38080
ORF Names: F16M14.1
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

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Protein attributes

Sequence length558 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Lignin degradation and detoxification of lignin-derived products By similarity. Required for secondary xylem cell wall lignification. Ref.3

Catalytic activity

4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.

Cofactor

Binds 4 copper ions per monomer By similarity.

Subcellular location

Secreted › extracellular space › apoplast Potential.

Tissue specificity

Ubiquitous, with higher levels in the inflorescence stem. Ref.4 Ref.5

Developmental stage

Transcript levels diminished during rosette leaves development. Ref.4

Sequence similarities

Belongs to the multicopper oxidase family.

Contains 3 plastocyanin-like domains.

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Ontologies

Keywords
   Biological processLignin degradation
   Cellular componentApoplast
Secreted
   DomainRepeat
Signal
   LigandCopper
Metal-binding
   Molecular functionOxidoreductase
   PTMGlycoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processlignin catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

oxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

secondary cell wall biogenesis Ref.3

Inferred from mutant phenotype. Source: TAIR

   Cellular componentapoplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functioncopper ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

laccase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Chain25 – 558534Laccase-4
PRO_0000283632

Regions

Domain32 – 148117Plastocyanin-like 1
Domain158 – 308151Plastocyanin-like 2
Domain408 – 542135Plastocyanin-like 3

Sites

Metal binding821Copper 1; type 2 By similarity
Metal binding841Copper 2; type 3 By similarity
Metal binding1271Copper 2; type 3 By similarity
Metal binding1291Copper 3; type 3 By similarity
Metal binding4591Copper 4; type 1 By similarity
Metal binding4621Copper 1; type 2 By similarity
Metal binding4641Copper 3; type 3 By similarity
Metal binding5211Copper 3; type 3 By similarity
Metal binding5221Copper 4; type 1 By similarity
Metal binding5231Copper 2; type 3 By similarity
Metal binding5271Copper 4; type 1 By similarity

Amino acid modifications

Glycosylation371N-linked (GlcNAc...) Potential
Glycosylation781N-linked (GlcNAc...) Potential
Glycosylation1141N-linked (GlcNAc...) Potential
Glycosylation1871N-linked (GlcNAc...) Potential
Glycosylation2961N-linked (GlcNAc...) Potential
Glycosylation3231N-linked (GlcNAc...) Potential
Glycosylation3301N-linked (GlcNAc...) Potential
Glycosylation3731N-linked (GlcNAc...) Potential
Glycosylation3831N-linked (GlcNAc...) Potential
Glycosylation4001N-linked (GlcNAc...) Potential
Glycosylation4181N-linked (GlcNAc...) Potential
Glycosylation4411N-linked (GlcNAc...) Potential
Glycosylation4791N-linked (GlcNAc...) Potential
Glycosylation5451N-linked (GlcNAc...) Potential

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Sequences

Sequence LengthMass (Da)Tools
O80434-1 [UniParc].

Last modified June 1, 2002. Version 2.
Checksum: C3BA6184D88E87DC

FASTA55861,527
        10         20         30         40         50         60 
MGSHMVWFLF LVSFFSVFPA PSESMVRHYK FNVVMKNVTR LCSSKPTVTV NGRYPGPTIY 

        70         80         90        100        110        120 
AREDDTLLIK VVNHVKYNVS IHWHGVRQVR TGWADGPAYI TQCPIQPGQV YTYNYTLTGQ 

       130        140        150        160        170        180 
RGTLWWHAHI LWLRATVYGA LVILPKRGVP YPFPKPDNEK VIVLGEWWKS DTENIINEAL 

       190        200        210        220        230        240 
KSGLAPNVSD SHMINGHPGP VRNCPSQGYK LSVENGKTYL LRLVNAALNE ELFFKVAGHI 

       250        260        270        280        290        300 
FTVVEVDAVY VKPFKTDTVL IAPGQTTNVL LTASKSAGKY LVTASPFMDA PIAVDNVTAT 

       310        320        330        340        350        360 
ATVHYSGTLS SSPTILTLPP PQNATSIANN FTNSLRSLNS KKYPALVPTT IDHHLFFTVG 

       370        380        390        400        410        420 
LGLNACPTCK AGNGSRVVAS INNVTFIMPK TALLPAHYFN TSGVFTTDFP KNPPHVFNYS 

       430        440        450        460        470        480 
GGSVTNMATE TGTRLYKLPY NATVQLVLQD TGVIAPENHP VHLHGFNFFE VGRGLGNFNS 

       490        500        510        520        530        540 
TKDPKNFNLV DPVERNTIGV PSGGWVVIRF RADNPGVWFM HCHLEVHTTW GLKMAFLVEN 

       550 
GKGPNQSILP PPKDLPKC

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References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed: 10617197] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[3]"Identification of novel genes in Arabidopsis involved in secondary cell wall formation using expression profiling and reverse genetics."
Brown D.M., Zeef L.A.H., Ellis J., Goodacre R., Turner S.R.
Plant Cell 17:2281-2295(2005) [PubMed: 15980264] [Abstract]
Cited for: FUNCTION.
[4]"Gene structure and molecular analysis of the laccase-like multicopper oxidase (LMCO) gene family in Arabidopsis thaliana."
McCaig B.C., Meagher R.B., Dean J.F.D.
Planta 221:619-636(2005) [PubMed: 15940465] [Abstract]
Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[5]"Mutant identification and characterization of the laccase gene family in Arabidopsis."
Cai X., Davis E.J., Ballif J., Liang M., Bushman E., Haroldsen V., Torabinejad J., Wu Y.
J. Exp. Bot. 57:2563-2569(2006) [PubMed: 16804053] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AC003028 Genomic DNA. Translation: AAC27158.2.
AY052669 mRNA. Translation: AAK96573.1.
AY063730 mRNA. Translation: AAL36080.1.
AY065187 mRNA. Translation: AAL38363.1.
AY114636 mRNA. Translation: AAM47955.1.
IPIIPI00522344.
PIRT01240.
RefSeqNP_565881.1.
UniGeneAt.12454

3D structure databases

ModBaseSearch...

Proteomic databases

PRIDEO80434.

Genome annotation databases

GeneID818386.
GenomeReviewsGene locus AT2G38080 in contig CT485783_GR.
KEGGath:AT2G38080.
NMPDRfig|3702.1.peg.10916.

Organism-specific databases

TAIRAt2g38080.

Phylogenomic databases

eggNOGKOG1263.
HOGENOMHBG749556.
InParanoidO80434.
OMAYAREDDT.
PhylomeDBO80434.

Enzyme and pathway databases

BRENDA1.10.3.2. 302.

Gene expression databases

ArrayExpressO80434.
GenevestigatorO80434.

Family and domain databases

InterProIPR001117. Cu-oxidase.
IPR011706. Cu-oxidase_2.
IPR011707. Cu-oxidase_3.
IPR002355. Cu_oxidase_Cu_BS.
IPR008972. Cupredoxin.
IPR017761. Laccase.
[Graphical view]
Gene3DG3DSA:2.60.40.420. Cupredoxin. 3 hits.
PfamPF00394. Cu-oxidase. 1 hit.
PF07731. Cu-oxidase_2. 1 hit.
PF07732. Cu-oxidase_3. 1 hit.
[Graphical view]
TIGRFAMsTIGR03389. laccase. 1 hit.
PROSITEPS00079. MULTICOPPER_OXIDASE1. 1 hit.
PS00080. MULTICOPPER_OXIDASE2. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameLAC4_ARATH
AccessionPrimary (citable) accession number: O80434
Secondary accession number(s): Q940U7
Entry history
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: June 1, 2002
Last modified: February 9, 2010
This is version 67 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

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Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents