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O80434

- LAC4_ARATH

UniProt

O80434 - LAC4_ARATH

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Protein

Laccase-4

Gene

IRX12

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Lignin degradation and detoxification of lignin-derived products (By similarity). Required for secondary xylem cell wall lignification.By similarity1 Publication

Catalytic activityi

4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.

Cofactori

Cu cationBy similarityNote: Binds 4 Cu cations per monomer.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi82 – 821Copper 1; type 2By similarity
Metal bindingi84 – 841Copper 2; type 3By similarity
Metal bindingi127 – 1271Copper 2; type 3By similarity
Metal bindingi129 – 1291Copper 3; type 3By similarity
Metal bindingi459 – 4591Copper 4; type 1By similarity
Metal bindingi462 – 4621Copper 1; type 2By similarity
Metal bindingi464 – 4641Copper 3; type 3By similarity
Metal bindingi521 – 5211Copper 3; type 3By similarity
Metal bindingi522 – 5221Copper 4; type 1By similarity
Metal bindingi523 – 5231Copper 2; type 3By similarity
Metal bindingi527 – 5271Copper 4; type 1By similarity

GO - Molecular functioni

  1. copper ion binding Source: InterPro
  2. hydroquinone:oxygen oxidoreductase activity Source: UniProtKB-EC
  3. oxidoreductase activity Source: TAIR

GO - Biological processi

  1. lignin biosynthetic process Source: TAIR
  2. lignin catabolic process Source: UniProtKB-KW
  3. secondary cell wall biogenesis Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lignin degradation

Keywords - Ligandi

Copper, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT2G38080-MONOMER.
MetaCyc:AT2G38080-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Laccase-4 (EC:1.10.3.2)
Alternative name(s):
Benzenediol:oxygen oxidoreductase 4
Diphenol oxidase 4
Protein IRREGULAR XYLEM 12
Urishiol oxidase 4
Gene namesi
Name:IRX12
Synonyms:LAC4
Ordered Locus Names:At2g38080
ORF Names:F16M14.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G38080.

Subcellular locationi

GO - Cellular componenti

  1. apoplast Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence AnalysisAdd
BLAST
Chaini25 – 558534Laccase-4PRO_0000283632Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi37 – 371N-linked (GlcNAc...)Sequence Analysis
Glycosylationi78 – 781N-linked (GlcNAc...)Sequence Analysis
Glycosylationi114 – 1141N-linked (GlcNAc...)Sequence Analysis
Glycosylationi187 – 1871N-linked (GlcNAc...)Sequence Analysis
Glycosylationi296 – 2961N-linked (GlcNAc...)Sequence Analysis
Glycosylationi323 – 3231N-linked (GlcNAc...)Sequence Analysis
Glycosylationi330 – 3301N-linked (GlcNAc...)Sequence Analysis
Glycosylationi373 – 3731N-linked (GlcNAc...)Sequence Analysis
Glycosylationi383 – 3831N-linked (GlcNAc...)Sequence Analysis
Glycosylationi400 – 4001N-linked (GlcNAc...)Sequence Analysis
Glycosylationi418 – 4181N-linked (GlcNAc...)Sequence Analysis
Glycosylationi441 – 4411N-linked (GlcNAc...)Sequence Analysis
Glycosylationi479 – 4791N-linked (GlcNAc...)Sequence Analysis
Glycosylationi545 – 5451N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiO80434.
PRIDEiO80434.

Expressioni

Tissue specificityi

Ubiquitous, with higher levels in the inflorescence stem.2 Publications

Developmental stagei

Transcript levels diminished during rosette leaves development.1 Publication

Gene expression databases

GenevestigatoriO80434.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G38080.1-P.

Structurei

3D structure databases

ProteinModelPortaliO80434.
SMRiO80434. Positions 24-537.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini32 – 148117Plastocyanin-like 1Add
BLAST
Domaini158 – 308151Plastocyanin-like 2Add
BLAST
Domaini408 – 542135Plastocyanin-like 3Add
BLAST

Sequence similaritiesi

Belongs to the multicopper oxidase family.Curated
Contains 3 plastocyanin-like domains.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiCOG2132.
HOGENOMiHOG000241916.
InParanoidiO80434.
OMAiVAGHIFT.
PhylomeDBiO80434.

Family and domain databases

Gene3Di2.60.40.420. 3 hits.
InterProiIPR001117. Cu-oxidase.
IPR011706. Cu-oxidase_2.
IPR011707. Cu-oxidase_3.
IPR002355. Cu_oxidase_Cu_BS.
IPR008972. Cupredoxin.
IPR017761. Laccase.
[Graphical view]
PfamiPF00394. Cu-oxidase. 1 hit.
PF07731. Cu-oxidase_2. 1 hit.
PF07732. Cu-oxidase_3. 1 hit.
[Graphical view]
SUPFAMiSSF49503. SSF49503. 3 hits.
TIGRFAMsiTIGR03389. laccase. 1 hit.
PROSITEiPS00079. MULTICOPPER_OXIDASE1. 1 hit.
PS00080. MULTICOPPER_OXIDASE2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O80434-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGSHMVWFLF LVSFFSVFPA PSESMVRHYK FNVVMKNVTR LCSSKPTVTV
60 70 80 90 100
NGRYPGPTIY AREDDTLLIK VVNHVKYNVS IHWHGVRQVR TGWADGPAYI
110 120 130 140 150
TQCPIQPGQV YTYNYTLTGQ RGTLWWHAHI LWLRATVYGA LVILPKRGVP
160 170 180 190 200
YPFPKPDNEK VIVLGEWWKS DTENIINEAL KSGLAPNVSD SHMINGHPGP
210 220 230 240 250
VRNCPSQGYK LSVENGKTYL LRLVNAALNE ELFFKVAGHI FTVVEVDAVY
260 270 280 290 300
VKPFKTDTVL IAPGQTTNVL LTASKSAGKY LVTASPFMDA PIAVDNVTAT
310 320 330 340 350
ATVHYSGTLS SSPTILTLPP PQNATSIANN FTNSLRSLNS KKYPALVPTT
360 370 380 390 400
IDHHLFFTVG LGLNACPTCK AGNGSRVVAS INNVTFIMPK TALLPAHYFN
410 420 430 440 450
TSGVFTTDFP KNPPHVFNYS GGSVTNMATE TGTRLYKLPY NATVQLVLQD
460 470 480 490 500
TGVIAPENHP VHLHGFNFFE VGRGLGNFNS TKDPKNFNLV DPVERNTIGV
510 520 530 540 550
PSGGWVVIRF RADNPGVWFM HCHLEVHTTW GLKMAFLVEN GKGPNQSILP

PPKDLPKC
Length:558
Mass (Da):61,527
Last modified:June 1, 2002 - v2
Checksum:iC3BA6184D88E87DC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC003028 Genomic DNA. Translation: AAC27158.2.
CP002685 Genomic DNA. Translation: AEC09487.1.
AY052669 mRNA. Translation: AAK96573.1.
AY063730 mRNA. Translation: AAL36080.1.
AY065187 mRNA. Translation: AAL38363.1.
AY114636 mRNA. Translation: AAM47955.1.
PIRiT01240.
RefSeqiNP_565881.1. NM_129364.3.
UniGeneiAt.12454.

Genome annotation databases

EnsemblPlantsiAT2G38080.1; AT2G38080.1; AT2G38080.
GeneIDi818386.
KEGGiath:AT2G38080.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC003028 Genomic DNA. Translation: AAC27158.2 .
CP002685 Genomic DNA. Translation: AEC09487.1 .
AY052669 mRNA. Translation: AAK96573.1 .
AY063730 mRNA. Translation: AAL36080.1 .
AY065187 mRNA. Translation: AAL38363.1 .
AY114636 mRNA. Translation: AAM47955.1 .
PIRi T01240.
RefSeqi NP_565881.1. NM_129364.3.
UniGenei At.12454.

3D structure databases

ProteinModelPortali O80434.
SMRi O80434. Positions 24-537.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT2G38080.1-P.

Proteomic databases

PaxDbi O80434.
PRIDEi O80434.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G38080.1 ; AT2G38080.1 ; AT2G38080 .
GeneIDi 818386.
KEGGi ath:AT2G38080.

Organism-specific databases

TAIRi AT2G38080.

Phylogenomic databases

eggNOGi COG2132.
HOGENOMi HOG000241916.
InParanoidi O80434.
OMAi VAGHIFT.
PhylomeDBi O80434.

Enzyme and pathway databases

BioCyci ARA:AT2G38080-MONOMER.
MetaCyc:AT2G38080-MONOMER.

Gene expression databases

Genevestigatori O80434.

Family and domain databases

Gene3Di 2.60.40.420. 3 hits.
InterProi IPR001117. Cu-oxidase.
IPR011706. Cu-oxidase_2.
IPR011707. Cu-oxidase_3.
IPR002355. Cu_oxidase_Cu_BS.
IPR008972. Cupredoxin.
IPR017761. Laccase.
[Graphical view ]
Pfami PF00394. Cu-oxidase. 1 hit.
PF07731. Cu-oxidase_2. 1 hit.
PF07732. Cu-oxidase_3. 1 hit.
[Graphical view ]
SUPFAMi SSF49503. SSF49503. 3 hits.
TIGRFAMsi TIGR03389. laccase. 1 hit.
PROSITEi PS00079. MULTICOPPER_OXIDASE1. 1 hit.
PS00080. MULTICOPPER_OXIDASE2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Identification of novel genes in Arabidopsis involved in secondary cell wall formation using expression profiling and reverse genetics."
    Brown D.M., Zeef L.A.H., Ellis J., Goodacre R., Turner S.R.
    Plant Cell 17:2281-2295(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Gene structure and molecular analysis of the laccase-like multicopper oxidase (LMCO) gene family in Arabidopsis thaliana."
    McCaig B.C., Meagher R.B., Dean J.F.D.
    Planta 221:619-636(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  6. "Mutant identification and characterization of the laccase gene family in Arabidopsis."
    Cai X., Davis E.J., Ballif J., Liang M., Bushman E., Haroldsen V., Torabinejad J., Wu Y.
    J. Exp. Bot. 57:2563-2569(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiLAC4_ARATH
AccessioniPrimary (citable) accession number: O80434
Secondary accession number(s): Q940U7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: June 1, 2002
Last modified: November 26, 2014
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3