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O80433 (CISY_DAUCA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Citrate synthase, mitochondrial

EC=2.3.3.16
Gene names
Name:CS
OrganismDaucus carota (Carrot)
Taxonomic identifier4039 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridscampanulidsApialesApiaceaeApioideaeScandiceaeDaucinaeDaucus

Protein attributes

Sequence length472 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.

Pathway

Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2.

Subunit structure

Homodimer By similarity.

Subcellular location

Mitochondrion matrix.

Miscellaneous

Citrate synthase is found in nearly all cells capable of oxidative metabolism.

Sequence similarities

Belongs to the citrate synthase family.

Ontologies

Keywords
   Biological processTricarboxylic acid cycle
   Cellular componentMitochondrion
   DomainTransit peptide
   Molecular functionTransferase
Gene Ontology (GO)
   Biological_processcellular carbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentmitochondrial matrix

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncitrate (Si)-synthase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Mitochondrion Potential
Chain? – 472Citrate synthase, mitochondrialPRO_0000005487

Sites

Active site3081 By similarity
Active site3541 By similarity
Active site4091 By similarity

Sequences

Sequence LengthMass (Da)Tools
O80433 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: A6C8CFCA17142120

FASTA47252,657
        10         20         30         40         50         60 
MVFFRSVSLL NKLRSRAVQQ SNLSNTVRWF QVQTSASDLD LRSQLKELIP EQQERIKKLK 

        70         80         90        100        110        120 
AEHGKVQLGN ITVDMVLGGM RGMTGLLWET SLLDPEEGIR FRGLSIPECQ KLLPGAKPGG 

       130        140        150        160        170        180 
EPLPEGLLWL LLTGKVPTKE QVDALSAELR SRAAVPEHVY KTIDALPVTA HPMTQFATGV 

       190        200        210        220        230        240 
MALQVQSEFQ KAYEKGIHKT KYWEPTYEDS ITLIAQLPVV AAYIYRRMYK NGQSISTDDS 

       250        260        270        280        290        300 
LDYGANFAHM LGYDSPSMQE LMRLYVTIHT DHEGGNVSAH TGHLVASALS DPYLSFAAAL 

       310        320        330        340        350        360 
NGLAGPLHGL ANQEVLLWIK SVVSECGENV TKEQLKDYIW KTLNSGKVVP GYGHGVLRNT 

       370        380        390        400        410        420 
DPRYICQREF ALKHLPDDPL FQLVSNLFEV VPPILTELGK VKNPWPNVDA HSGVLLNHYG 

       430        440        450        460        470 
LTEARYYTVL FGVSRAIGIC SQLVWDRALG LPLERPKSVT MEWLENHCKK SS 

« Hide

References

[1]"cDNA encoding carrot mitochondrial citrate synthase."
Takita E., Koyama H., Shirano Y., Shibata D., Hara T.
Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. MS Yonsun.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB017159 mRNA. Translation: BAA32557.1.

3D structure databases

ProteinModelPortalO80433.
SMRO80433. Positions 36-464.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEO80433.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-15824.
BRENDA2.3.3.1. 1841.
UniPathwayUPA00223; UER00717.

Family and domain databases

Gene3D1.10.580.10. 1 hit.
InterProIPR016142. Citrate_synth-like_lrg_a-sub.
IPR002020. Citrate_synthase-like.
IPR016141. Citrate_synthase-like_core.
IPR019810. Citrate_synthase_AS.
IPR010109. Citrate_synthase_euk.
[Graphical view]
PANTHERPTHR11739. PTHR11739. 1 hit.
PfamPF00285. Citrate_synt. 1 hit.
[Graphical view]
PRINTSPR00143. CITRTSNTHASE.
SUPFAMSSF48256. SSF48256. 1 hit.
TIGRFAMsTIGR01793. cit_synth_euk. 1 hit.
PROSITEPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCISY_DAUCA
AccessionPrimary (citable) accession number: O80433
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1998
Last modified: June 11, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways