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Protein

Mitogen-activated protein kinase kinase 3

Gene

MKK3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

MKK3-MPK6 module plays an important role in the jasmonate signal transduction pathway through the negative regulation of MYC2/JIN1 expression. Activates by phosphorylation the downstream MPK6, MPK7 and MPK8. MKK3-MPK7 module acts as a positive regulator of PR1 gene expression. MKK3-MPK8 module negatively regulates ROS accumulation through controlling expression of the RBOHD gene. Component of the abscisic acid (ABA) signaling pathway that may act as ABA signal transducer in the context of abiotic stresses. Activator of the C group MAP kinases. Activates MPK7 in response to ABA (PubMed:25720833).4 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei112ATPPROSITE-ProRule annotation1
Active sitei207Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi89 – 97ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • MAP kinase activity Source: TAIR
  • MAP kinase kinase activity Source: TAIR

GO - Biological processi

  • abscisic acid-activated signaling pathway Source: UniProtKB
  • defense response, incompatible interaction Source: TAIR
  • induced systemic resistance, ethylene mediated signaling pathway Source: TAIR
  • induced systemic resistance, jasmonic acid mediated signaling pathway Source: TAIR
  • regulation of mitotic cell cycle Source: GO_Central
  • response to abscisic acid Source: UniProtKB
  • stress-activated protein kinase signaling cascade Source: GO_Central

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processAbscisic acid signaling pathway, Plant defense
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase kinase 3 (EC:2.7.12.2)
Short name:
AtMKK3
Short name:
MAP kinase kinase 3
Gene namesi
Name:MKK3
Ordered Locus Names:At5g40440
ORF Names:MPO12.19, MPO12_150
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G40440
TAIRilocus:2170578 AT5G40440

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Disruption phenotypei

More sensitive to Pseudomonas syringae pv. tomato DC3000 infection. Hypersensitivity to abscisic acid (ABA) in germination and root elongation (PubMed:25720833).2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi235S → D: Constitutively active; when associated with D-241. 3 Publications1
Mutagenesisi235S → E: Constitutively active; when associated with E-241. 3 Publications1
Mutagenesisi241T → D: Constitutively active; when associated with D-235. 3 Publications1
Mutagenesisi241T → E: Constitutively active; when associated with E-235. 3 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004286221 – 520Mitogen-activated protein kinase kinase 3Add BLAST520

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei69PhosphoserineBy similarity1
Modified residuei235Phosphoserine3 Publications1
Modified residuei241Phosphothreonine3 Publications1
Modified residuei245PhosphothreonineBy similarity1

Post-translational modificationi

Phosphorylation at Ser-235 and Thr-241 by MAP kinase kinase kinases positively regulates kinase activity.3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO80396
PRIDEiO80396

PTM databases

iPTMnetiO80396

Expressioni

Tissue specificityi

Ubiquitous.2 Publications

Inductioni

By Pseudomonas syringae pv tomato strain DC3000 infection.1 Publication

Gene expression databases

ExpressionAtlasiO80396 baseline and differential
GenevisibleiO80396 AT

Interactioni

Subunit structurei

Interacts with MPK1, MPK2 and MPK7 (PubMed:17933903, PubMed:19513235, PubMed:25680457). Interacts with P.syringae type III effector HopF2 (PubMed:20571112). Interacts with MPK14 (PubMed:19513235). Binds to MAPKKK17 and MAPKKK18 (PubMed:25720833, PubMed:25680457).5 Publications

Protein-protein interaction databases

BioGridi19293, 6 interactors
IntActiO80396, 5 interactors
STRINGi3702.AT5G40440.1

Structurei

3D structure databases

ProteinModelPortaliO80396
SMRiO80396
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini83 – 339Protein kinasePROSITE-ProRule annotationAdd BLAST257
Domaini366 – 516NTF2PROSITE-ProRule annotationAdd BLAST151

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi22 – 26Poly-Ser5

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0581 Eukaryota
ENOG410XQ5A LUCA
HOGENOMiHOG000234206
InParanoidiO80396
KOiK20607
OMAiCEAPCYQ
OrthoDBiEOG093605XY
PhylomeDBiO80396

Family and domain databases

CDDicd00780 NTF2, 1 hit
InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR032710 NTF2-like_dom_sf
IPR018222 Nuclear_transport_factor_2_euk
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF54427 SSF54427, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50177 NTF2_DOMAIN, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

O80396-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAALEELKKK LSPLFDAEKG FSSSSSLDPN DSYLLSDGGT VNLLSRSYGV
60 70 80 90 100
YNFNELGLQK CTSSHVDESE SSETTYQCAS HEMRVFGAIG SGASSVVQRA
110 120 130 140 150
IHIPNHRILA LKKINIFERE KRQQLLTEIR TLCEAPCHEG LVDFHGAFYS
160 170 180 190 200
PDSGQISIAL EYMNGGSLAD ILKVTKKIPE PVLSSLFHKL LQGLSYLHGV
210 220 230 240 250
RHLVHRDIKP ANLLINLKGE PKITDFGISA GLENSMAMCA TFVGTVTYMS
260 270 280 290 300
PERIRNDSYS YPADIWSLGL ALFECGTGEF PYIANEGPVN LMLQILDDPS
310 320 330 340 350
PTPPKQEFSP EFCSFIDACL QKDPDARPTA DQLLSHPFIT KHEKERVDLA
360 370 380 390 400
TFVQSIFDPT QRLKDLADML TIHYYSLFDG FDDLWHHAKS LYTETSVFSF
410 420 430 440 450
SGKHNTGSTE IFSALSDIRN TLTGDLPSEK LVHVVEKLHC KPCGSGGVII
460 470 480 490 500
RAVGSFIVGN QFLICGDGVQ AEGLPSFKDL GFDVASRRVG RFQEQFVVES
510 520
GDLIGKYFLA KQELYITNLD
Length:520
Mass (Da):57,529
Last modified:November 1, 1998 - v1
Checksum:i9CD63397CCF38AA2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB015314 mRNA Translation: BAA28829.1
AB006702 Genomic DNA Translation: BAB11601.1
CP002688 Genomic DNA Translation: AED94548.1
CP002688 Genomic DNA Translation: ANM70658.1
AK117136 mRNA Translation: BAC41814.1
BT006481 mRNA Translation: AAP21289.1
PIRiT51338
RefSeqiNP_001318713.1, NM_001344328.1
NP_198860.1, NM_123408.3
UniGeneiAt.350

Genome annotation databases

EnsemblPlantsiAT5G40440.1; AT5G40440.1; AT5G40440
AT5G40440.4; AT5G40440.4; AT5G40440
GeneIDi834042
GrameneiAT5G40440.1; AT5G40440.1; AT5G40440
AT5G40440.4; AT5G40440.4; AT5G40440
KEGGiath:AT5G40440

Similar proteinsi

Entry informationi

Entry nameiM2K3_ARATH
AccessioniPrimary (citable) accession number: O80396
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: November 1, 1998
Last modified: April 25, 2018
This is version 147 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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