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Protein

Mitogen-activated protein kinase kinase 3

Gene

MKK3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

MKK3-MPK6 module plays an important role in the jasmonate signal transduction pathway through the negative regulation of MYC2/JIN1 expression. Activates by phosphorylation the downstream MPK6, MPK7 and MPK8. MKK3-MPK7 module acts as a positive regulator of PR1 gene expression. MKK3-MPK8 module negatively regulates ROS accumulation through controlling expression of the RBOHD gene.3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei112ATPPROSITE-ProRule annotation1
Active sitei207Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi89 – 97ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • MAP kinase activity Source: TAIR
  • MAP kinase kinase activity Source: TAIR

GO - Biological processi

  • defense response, incompatible interaction Source: TAIR
  • induced systemic resistance, ethylene mediated signaling pathway Source: TAIR
  • induced systemic resistance, jasmonic acid mediated signaling pathway Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Plant defense

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase kinase 3 (EC:2.7.12.2)
Short name:
AtMKK3
Short name:
MAP kinase kinase 3
Gene namesi
Name:MKK3
Ordered Locus Names:At5g40440
ORF Names:MPO12.19, MPO12_150
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G40440.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

More sensitive to Pseudomonas syringae pv. tomato DC3000 infection.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi235S → D: Constitutively active; when associated with D-241. 3 Publications1
Mutagenesisi235S → E: Constitutively active; when associated with E-241. 3 Publications1
Mutagenesisi241T → D: Constitutively active; when associated with D-235. 3 Publications1
Mutagenesisi241T → E: Constitutively active; when associated with E-235. 3 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004286221 – 520Mitogen-activated protein kinase kinase 3Add BLAST520

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei69PhosphoserineBy similarity1
Modified residuei235Phosphoserine3 Publications1
Modified residuei241Phosphothreonine3 Publications1
Modified residuei245PhosphothreonineBy similarity1

Post-translational modificationi

Phosphorylation at Ser-235 and Thr-241 by MAP kinase kinase kinases positively regulates kinase activity.3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO80396.
PRIDEiO80396.

PTM databases

iPTMnetiO80396.

Expressioni

Tissue specificityi

Ubiquitous.2 Publications

Inductioni

By Pseudomonas syringae pv tomato strain DC3000 infection.1 Publication

Gene expression databases

GenevisibleiO80396. AT.

Interactioni

Subunit structurei

Interacts with MPK1, MPK2 and MPK7. Interacts with P.syringae type III effector HopF2. Interacts with MPK14.3 Publications

Protein-protein interaction databases

BioGridi19293. 6 interactors.
IntActiO80396. 5 interactors.
STRINGi3702.AT5G40440.1.

Structurei

3D structure databases

ProteinModelPortaliO80396.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini83 – 339Protein kinasePROSITE-ProRule annotationAdd BLAST257
Domaini366 – 516NTF2PROSITE-ProRule annotationAdd BLAST151

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi22 – 26Poly-Ser5

Sequence similaritiesi

Contains 1 NTF2 domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0581. Eukaryota.
ENOG410XQ5A. LUCA.
HOGENOMiHOG000234206.
InParanoidiO80396.
KOiK20607.
OMAiCASHEMR.
OrthoDBiEOG093605XY.
PhylomeDBiO80396.

Family and domain databases

CDDicd00780. NTF2. 1 hit.
Gene3Di3.10.450.50. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR032710. NTF2-like_dom.
IPR018222. Nuclear_transport_factor_2_euk.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF54427. SSF54427. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50177. NTF2_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O80396-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAALEELKKK LSPLFDAEKG FSSSSSLDPN DSYLLSDGGT VNLLSRSYGV
60 70 80 90 100
YNFNELGLQK CTSSHVDESE SSETTYQCAS HEMRVFGAIG SGASSVVQRA
110 120 130 140 150
IHIPNHRILA LKKINIFERE KRQQLLTEIR TLCEAPCHEG LVDFHGAFYS
160 170 180 190 200
PDSGQISIAL EYMNGGSLAD ILKVTKKIPE PVLSSLFHKL LQGLSYLHGV
210 220 230 240 250
RHLVHRDIKP ANLLINLKGE PKITDFGISA GLENSMAMCA TFVGTVTYMS
260 270 280 290 300
PERIRNDSYS YPADIWSLGL ALFECGTGEF PYIANEGPVN LMLQILDDPS
310 320 330 340 350
PTPPKQEFSP EFCSFIDACL QKDPDARPTA DQLLSHPFIT KHEKERVDLA
360 370 380 390 400
TFVQSIFDPT QRLKDLADML TIHYYSLFDG FDDLWHHAKS LYTETSVFSF
410 420 430 440 450
SGKHNTGSTE IFSALSDIRN TLTGDLPSEK LVHVVEKLHC KPCGSGGVII
460 470 480 490 500
RAVGSFIVGN QFLICGDGVQ AEGLPSFKDL GFDVASRRVG RFQEQFVVES
510 520
GDLIGKYFLA KQELYITNLD
Length:520
Mass (Da):57,529
Last modified:November 1, 1998 - v1
Checksum:i9CD63397CCF38AA2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB015314 mRNA. Translation: BAA28829.1.
AB006702 Genomic DNA. Translation: BAB11601.1.
CP002688 Genomic DNA. Translation: AED94548.1.
AK117136 mRNA. Translation: BAC41814.1.
BT006481 mRNA. Translation: AAP21289.1.
PIRiT51338.
RefSeqiNP_198860.1. NM_123408.3.
UniGeneiAt.350.

Genome annotation databases

EnsemblPlantsiAT5G40440.1; AT5G40440.1; AT5G40440.
GeneIDi834042.
GrameneiAT5G40440.1; AT5G40440.1; AT5G40440.
KEGGiath:AT5G40440.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB015314 mRNA. Translation: BAA28829.1.
AB006702 Genomic DNA. Translation: BAB11601.1.
CP002688 Genomic DNA. Translation: AED94548.1.
AK117136 mRNA. Translation: BAC41814.1.
BT006481 mRNA. Translation: AAP21289.1.
PIRiT51338.
RefSeqiNP_198860.1. NM_123408.3.
UniGeneiAt.350.

3D structure databases

ProteinModelPortaliO80396.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi19293. 6 interactors.
IntActiO80396. 5 interactors.
STRINGi3702.AT5G40440.1.

PTM databases

iPTMnetiO80396.

Proteomic databases

PaxDbiO80396.
PRIDEiO80396.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G40440.1; AT5G40440.1; AT5G40440.
GeneIDi834042.
GrameneiAT5G40440.1; AT5G40440.1; AT5G40440.
KEGGiath:AT5G40440.

Organism-specific databases

TAIRiAT5G40440.

Phylogenomic databases

eggNOGiKOG0581. Eukaryota.
ENOG410XQ5A. LUCA.
HOGENOMiHOG000234206.
InParanoidiO80396.
KOiK20607.
OMAiCASHEMR.
OrthoDBiEOG093605XY.
PhylomeDBiO80396.

Miscellaneous databases

PROiO80396.

Gene expression databases

GenevisibleiO80396. AT.

Family and domain databases

CDDicd00780. NTF2. 1 hit.
Gene3Di3.10.450.50. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR032710. NTF2-like_dom.
IPR018222. Nuclear_transport_factor_2_euk.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF54427. SSF54427. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50177. NTF2_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiM2K3_ARATH
AccessioniPrimary (citable) accession number: O80396
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: November 1, 1998
Last modified: November 30, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.