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Protein

Formamidopyrimidine-DNA glycosylase

Gene

FPG1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Can process efficiently 4,6-diamino-5-formamidopyrimidine (FapyA), 2,6-diamino-4- hydroxy-5-formamidopyrimidine (FapyG) and the further oxidation products of 8-oxoguanine (8-oxoG), such as guanidinohydantoin and spiroiminodihydantoin. Has marginal activity towards 8-oxoG. Has AP (apurinic/apyrimidinic) lyase activity. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates.3 Publications

Catalytic activityi

Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine.1 Publication
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.PROSITE-ProRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2Schiff-base intermediate with DNAPROSITE-ProRule annotation1
Active sitei3Proton donorPROSITE-ProRule annotation1
Active sitei60Proton donor; for beta-elimination activityPROSITE-ProRule annotation1
Binding sitei107DNA1 Publication1
Binding sitei126DNABy similarity1
Binding sitei167DNA1 Publication1
Binding sitei186DNA1 Publication1

GO - Molecular functioni

GO - Biological processi

  • base-excision repair Source: InterPro
  • DNA repair Source: TAIR
  • nucleotide-excision repair Source: InterPro
  • response to oxidative stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Lyase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciARA:AT1G52500-MONOMER.
BRENDAi3.2.2.23. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
Formamidopyrimidine-DNA glycosylase (EC:3.2.2.23, EC:4.2.99.18)
Short name:
Fapy-DNA glycosylase
Alternative name(s):
DNA-(apurinic or apyrimidinic site) lyase FPG1
Formamidopyrimidine-DNA glycosylase 1
Short name:
AtFPG-1
Formamidopyrimidine-DNA glycosylase 2
Short name:
AtFPG-2
Protein MutM homolog 1
Short name:
AtMMH-1
Protein MutM homolog 2
Short name:
AtMMH-2
Gene namesi
Name:FPG1
Synonyms:FPG2
Ordered Locus Names:At1g52500
ORF Names:F6D8.28
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G52500.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions or UV-A irradiation stress.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00004212612 – 390Formamidopyrimidine-DNA glycosylaseAdd BLAST389

Proteomic databases

PaxDbiO80358.

PTM databases

iPTMnetiO80358.

Expressioni

Tissue specificityi

Expressed in leaves (at protein levels).1 Publication

Gene expression databases

GenevisibleiO80358. AT.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

STRINGi3702.AT1G52500.2.

Structurei

Secondary structure

1390
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 17Combined sources14
Turni18 – 20Combined sources3
Beta strandi22 – 28Combined sources7
Turni32 – 34Combined sources3
Helixi40 – 47Combined sources8
Beta strandi51 – 58Combined sources8
Beta strandi61 – 69Combined sources9
Beta strandi71 – 75Combined sources5
Turni77 – 79Combined sources3
Beta strandi81 – 85Combined sources5
Helixi93 – 95Combined sources3
Beta strandi108 – 114Combined sources7
Helixi115 – 117Combined sources3
Beta strandi119 – 123Combined sources5
Beta strandi125 – 127Combined sources3
Beta strandi130 – 135Combined sources6
Helixi137 – 139Combined sources3
Helixi143 – 145Combined sources3
Turni150 – 152Combined sources3
Helixi157 – 165Combined sources9
Helixi171 – 176Combined sources6
Turni178 – 180Combined sources3
Beta strandi181 – 183Combined sources3
Helixi186 – 195Combined sources10
Helixi204 – 206Combined sources3
Helixi209 – 228Combined sources20
Turni229 – 231Combined sources3
Helixi233 – 235Combined sources3
Helixi241 – 244Combined sources4
Beta strandi260 – 264Combined sources5
Beta strandi267 – 271Combined sources5
Turni273 – 275Combined sources3
Helixi280 – 286Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3TWKX-ray2.30A/B1-281[»]
3TWLX-ray1.70A1-304[»]
3TWMX-ray2.80A/B1-304[»]
ProteinModelPortaliO80358.
SMRiO80358.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the FPG family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IKB3. Eukaryota.
COG0266. LUCA.
HOGENOMiHOG000191723.
InParanoidiO80358.
KOiK10563.
OMAiHAHVFFR.
OrthoDBiEOG09360C5A.
PhylomeDBiO80358.

Family and domain databases

InterProiIPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR020629. Formamido-pyr_DNA_Glyclase.
IPR012319. FPG_cat.
IPR010979. Ribosomal_S13-like_H2TH.
[Graphical view]
PfamiPF01149. Fapy_DNA_glyco. 1 hit.
PF06831. H2TH. 1 hit.
[Graphical view]
SMARTiSM00898. Fapy_DNA_glyco. 1 hit.
SM01232. H2TH. 1 hit.
[Graphical view]
SUPFAMiSSF46946. SSF46946. 1 hit.
SSF81624. SSF81624. 1 hit.
TIGRFAMsiTIGR00577. fpg. 1 hit.
PROSITEiPS51068. FPG_CAT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O80358-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPELPEVEAA RRAIEENCLG KKIKRVIIAD DNKVIHGISP SDFQTSILGK
60 70 80 90 100
TIISARRKGK NLWLELDSPP FPSFQFGMAG AIYIKGVAVT KYKRSAVKDS
110 120 130 140 150
EEWPSKYSKF FVELDDGLEL SFTDKRRFAK VRLLANPTSV SPISELGPDA
160 170 180 190 200
LLEPMTVDEF AESLAKKKIT IKPLLLDQGY ISGIGNWIAD EVLYQARIHP
210 220 230 240 250
LQTASSLSKE QCEALHTSIK EVIEKAVEVD ADSSQFPSYW IFHNREKKPG
260 270 280 290 300
KAFVDGKKID FITAGGRTTA YVPELQKLYG KDAEKAAKVR PAKRGVKPKE
310 320 330 340 350
DDGDGEEDEQ ETEKEDESAK SKKGQKPRGG RGKKPASKTK TEESDDDGDD
360 370 380 390
SEAEEEVVKP KGRGTKPAIK RKSEEKATSQ AGKKPKGRKS
Length:390
Mass (Da):43,194
Last modified:November 1, 1998 - v1
Checksum:iF6B5E0DAFD6FD010
GO
Isoform 2 (identifier: O80358-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     224-274: EKAVEVDADS...GGRTTAYVPE → QHAVQVNADS...LMKQNLGFCR
     275-390: Missing.

Note: No experimental confirmation available.
Show »
Length:274
Mass (Da):30,762
Checksum:iE1C7829A15E0D360
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti239Y → N in AAC97952 (PubMed:11535407).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_045377224 – 274EKAVE…AYVPE → QHAVQVNADSKEFPVEWLFH FRWGKKAGKVNGKLSHHLSI NLMKQNLGFCR in isoform 2. 1 PublicationAdd BLAST51
Alternative sequenceiVSP_045378275 – 390Missing in isoform 2. 1 PublicationAdd BLAST116

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB010690 Genomic DNA. Translation: BAA32702.1.
AB010690 Genomic DNA. Translation: BAA32703.1.
AF099970 mRNA. Translation: AAC97952.1.
AF099971 mRNA. Translation: AAC97953.1.
AC008016 Genomic DNA. Translation: AAD55612.1.
AC008016 Genomic DNA. Translation: AAD55613.1.
CP002684 Genomic DNA. Translation: AEE32814.1.
CP002684 Genomic DNA. Translation: AEE32815.1.
BT043496 mRNA. Translation: ACG58696.1.
PIRiE96565.
T51713.
T51714.
RefSeqiNP_564608.1. NM_104128.3. [O80358-1]
NP_849798.1. NM_179467.3. [O80358-2]
UniGeneiAt.11653.
At.46186.

Genome annotation databases

EnsemblPlantsiAT1G52500.2; AT1G52500.2; AT1G52500. [O80358-1]
GeneIDi841681.
GrameneiAT1G52500.2; AT1G52500.2; AT1G52500.
KEGGiath:AT1G52500.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB010690 Genomic DNA. Translation: BAA32702.1.
AB010690 Genomic DNA. Translation: BAA32703.1.
AF099970 mRNA. Translation: AAC97952.1.
AF099971 mRNA. Translation: AAC97953.1.
AC008016 Genomic DNA. Translation: AAD55612.1.
AC008016 Genomic DNA. Translation: AAD55613.1.
CP002684 Genomic DNA. Translation: AEE32814.1.
CP002684 Genomic DNA. Translation: AEE32815.1.
BT043496 mRNA. Translation: ACG58696.1.
PIRiE96565.
T51713.
T51714.
RefSeqiNP_564608.1. NM_104128.3. [O80358-1]
NP_849798.1. NM_179467.3. [O80358-2]
UniGeneiAt.11653.
At.46186.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3TWKX-ray2.30A/B1-281[»]
3TWLX-ray1.70A1-304[»]
3TWMX-ray2.80A/B1-304[»]
ProteinModelPortaliO80358.
SMRiO80358.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G52500.2.

PTM databases

iPTMnetiO80358.

Proteomic databases

PaxDbiO80358.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G52500.2; AT1G52500.2; AT1G52500. [O80358-1]
GeneIDi841681.
GrameneiAT1G52500.2; AT1G52500.2; AT1G52500.
KEGGiath:AT1G52500.

Organism-specific databases

TAIRiAT1G52500.

Phylogenomic databases

eggNOGiENOG410IKB3. Eukaryota.
COG0266. LUCA.
HOGENOMiHOG000191723.
InParanoidiO80358.
KOiK10563.
OMAiHAHVFFR.
OrthoDBiEOG09360C5A.
PhylomeDBiO80358.

Enzyme and pathway databases

BioCyciARA:AT1G52500-MONOMER.
BRENDAi3.2.2.23. 399.

Miscellaneous databases

PROiO80358.

Gene expression databases

GenevisibleiO80358. AT.

Family and domain databases

InterProiIPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR020629. Formamido-pyr_DNA_Glyclase.
IPR012319. FPG_cat.
IPR010979. Ribosomal_S13-like_H2TH.
[Graphical view]
PfamiPF01149. Fapy_DNA_glyco. 1 hit.
PF06831. H2TH. 1 hit.
[Graphical view]
SMARTiSM00898. Fapy_DNA_glyco. 1 hit.
SM01232. H2TH. 1 hit.
[Graphical view]
SUPFAMiSSF46946. SSF46946. 1 hit.
SSF81624. SSF81624. 1 hit.
TIGRFAMsiTIGR00577. fpg. 1 hit.
PROSITEiPS51068. FPG_CAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFPG_ARATH
AccessioniPrimary (citable) accession number: O80358
Secondary accession number(s): O80359, Q9SBB4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 6, 2013
Last sequence update: November 1, 1998
Last modified: November 30, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.