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Protein

Cyclin-dependent kinase F-1

Gene

CDKF-1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

CDK-activating kinase that modulates CDKD-2 and CDKD-3 activities by phosphorylation of the T-loop. Activates CDKD-2 C-terminal domain (CTD) kinase activity. Activates CDKA-1 probably by phosphorylation. Posseses a CDK kinase activity independently of association with cyclin CYCH1-1. Phosphorylates the CTD of the large subunit of RNA polymerase II.3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei50ATPPROSITE-ProRule annotation1
Active sitei146Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi27 – 35ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • maintenance of root meristem identity Source: TAIR
  • protein phosphorylation Source: TAIR
  • regulation of cyclin-dependent protein serine/threonine kinase activity Source: TAIR

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processCell cycle, Cell division
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase F-1 (EC:2.7.11.22, EC:2.7.11.23)
Short name:
CDKF;1
Alternative name(s):
CDK-activating kinase 1-At
Short name:
CAK1-At
Gene namesi
Name:CDKF-1
Synonyms:CAK1
Ordered Locus Names:At4g28980
ORF Names:F19B15.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G28980
TAIRilocus:2119961 AT4G28980

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi50K → A: No effect on CDKA-1 activation. 1 Publication1
Mutagenesisi290T → A: Abolishes CDKA-1 activation and kinase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002931231 – 479Cyclin-dependent kinase F-1Add BLAST479

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei32PhosphotyrosineBy similarity1
Modified residuei179PhosphoserineCombined sources1
Modified residuei208PhosphoserineBy similarity1
Modified residuei247PhosphoserineCombined sources1
Modified residuei290Phosphothreonine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO80345
PRIDEiO80345

PTM databases

iPTMnetiO80345

Expressioni

Tissue specificityi

Highly expressed in suspension cell culture. Expressed at low levels in all plant organs.1 Publication

Gene expression databases

ExpressionAtlasiO80345 differential
GenevisibleiO80345 AT

Interactioni

Protein-protein interaction databases

BioGridi14306, 30 interactors
IntActiO80345, 5 interactors
STRINGi3702.AT4G28980.1

Structurei

3D structure databases

ProteinModelPortaliO80345
SMRiO80345
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 418Protein kinasePROSITE-ProRule annotationAdd BLAST398

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0594 Eukaryota
ENOG410XPP3 LUCA
HOGENOMiHOG000233024
InParanoidiO80345
OMAiARILMEP
OrthoDBiEOG09360A7L
PhylomeDBiO80345

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 2 hits
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 2 hits
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

O80345-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKQPATSWS IHTRPEIIAK YEIFERVGSG AYADVYRARR LSDGLIVALK
60 70 80 90 100
EIFDYQSAFR EIDALTILNG SPNVVVMHEY FWREEENAVL VLEFLRSDLA
110 120 130 140 150
AVIRDGKRKK KVEGGDGFSV GEIKRWMIQI LTGVDACHRN LIVHRDLKPG
160 170 180 190 200
NMLISDDGVL KLADFGQARI LMEHDIVASD ENQQAYKLED KDGETSEPPE
210 220 230 240 250
VIPDYENSPR QGSDGQEREA MSKDEYFRQV EELKAKQVVR DDTDKDSNVH
260 270 280 290 300
DGDISCLATC TVSEMDDDLG RNSFSYDADE AVDDTQGLMT SCVGTRWFRP
310 320 330 340 350
PELLYGSTMY GLEVDLWSLG CVFAELLSLE PLFPGISDID QISRVTNVLG
360 370 380 390 400
NLNEEVWPGC VDLPDYKSIS FAKVESPLGI EGCLPNHSGD VISLLKKLIC
410 420 430 440 450
YDPASRATTM EMLNDKYLSE EPLPVPVSEL YVPPTMSGPD EDSPRKWNDY
460 470
REMDSDSDFD GFGPMNVKPT SSGFTIEFP
Length:479
Mass (Da):53,752
Last modified:November 1, 1998 - v1
Checksum:iCA08BF178B7CC831
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB009399 mRNA Translation: BAA28775.1
AL078470 Genomic DNA Translation: CAB43912.1
AL161574 Genomic DNA Translation: CAB79656.1
CP002687 Genomic DNA Translation: AEE85567.1
CP002687 Genomic DNA Translation: AEE85568.1
CP002687 Genomic DNA Translation: ANM67753.1
AY042816 mRNA Translation: AAK68756.1
AY064627 mRNA Translation: AAL47340.1
PIRiT08953
RefSeqiNP_001329562.1, NM_001341955.1
NP_194627.1, NM_119042.5
NP_849468.1, NM_179137.3
UniGeneiAt.19673

Genome annotation databases

EnsemblPlantsiAT4G28980.1; AT4G28980.1; AT4G28980
AT4G28980.2; AT4G28980.2; AT4G28980
AT4G28980.3; AT4G28980.3; AT4G28980
GeneIDi829019
GrameneiAT4G28980.1; AT4G28980.1; AT4G28980
AT4G28980.2; AT4G28980.2; AT4G28980
AT4G28980.3; AT4G28980.3; AT4G28980
KEGGiath:AT4G28980

Similar proteinsi

Entry informationi

Entry nameiCDKF1_ARATH
AccessioniPrimary (citable) accession number: O80345
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: November 1, 1998
Last modified: May 23, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families
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