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Protein

Cyclin-dependent kinase F-1

Gene

CDKF-1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

CDK-activating kinase that modulates CDKD-2 and CDKD-3 activities by phosphorylation of the T-loop. Activates CDKD-2 C-terminal domain (CTD) kinase activity. Activates CDKA-1 probably by phosphorylation. Posseses a CDK kinase activity independently of association with cyclin CYCH1-1. Phosphorylates the CTD of the large subunit of RNA polymerase II.3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei50ATPPROSITE-ProRule annotation1
Active sitei146Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi27 – 35ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • maintenance of root meristem identity Source: TAIR
  • protein phosphorylation Source: TAIR
  • regulation of cyclin-dependent protein serine/threonine kinase activity Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, Cell division

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase F-1 (EC:2.7.11.22, EC:2.7.11.23)
Short name:
CDKF;1
Alternative name(s):
CDK-activating kinase 1-At
Short name:
CAK1-At
Gene namesi
Name:CDKF-1
Synonyms:CAK1
Ordered Locus Names:At4g28980
ORF Names:F19B15.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G28980.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: TAIR
  • cytosol Source: TAIR
  • nucleus Source: TAIR
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi50K → A: No effect on CDKA-1 activation. 1 Publication1
Mutagenesisi290T → A: Abolishes CDKA-1 activation and kinase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002931231 – 479Cyclin-dependent kinase F-1Add BLAST479

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei32PhosphotyrosineBy similarity1
Modified residuei179PhosphoserineCombined sources1
Modified residuei208PhosphoserineBy similarity1
Modified residuei247PhosphoserineCombined sources1
Modified residuei290Phosphothreonine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO80345.
PRIDEiO80345.

PTM databases

iPTMnetiO80345.

Expressioni

Tissue specificityi

Highly expressed in suspension cell culture. Expressed at low levels in all plant organs.1 Publication

Gene expression databases

ExpressionAtlasiO80345. baseline and differential.
GenevisibleiO80345. AT.

Interactioni

Protein-protein interaction databases

BioGridi14306. 30 interactors.
IntActiO80345. 5 interactors.
STRINGi3702.AT4G28980.1.

Structurei

3D structure databases

ProteinModelPortaliO80345.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 418Protein kinasePROSITE-ProRule annotationAdd BLAST398

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0594. Eukaryota.
ENOG410XPP3. LUCA.
HOGENOMiHOG000233024.
InParanoidiO80345.
OMAiPGFVAND.
OrthoDBiEOG09360A7L.
PhylomeDBiO80345.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 2 hits.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O80345-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKQPATSWS IHTRPEIIAK YEIFERVGSG AYADVYRARR LSDGLIVALK
60 70 80 90 100
EIFDYQSAFR EIDALTILNG SPNVVVMHEY FWREEENAVL VLEFLRSDLA
110 120 130 140 150
AVIRDGKRKK KVEGGDGFSV GEIKRWMIQI LTGVDACHRN LIVHRDLKPG
160 170 180 190 200
NMLISDDGVL KLADFGQARI LMEHDIVASD ENQQAYKLED KDGETSEPPE
210 220 230 240 250
VIPDYENSPR QGSDGQEREA MSKDEYFRQV EELKAKQVVR DDTDKDSNVH
260 270 280 290 300
DGDISCLATC TVSEMDDDLG RNSFSYDADE AVDDTQGLMT SCVGTRWFRP
310 320 330 340 350
PELLYGSTMY GLEVDLWSLG CVFAELLSLE PLFPGISDID QISRVTNVLG
360 370 380 390 400
NLNEEVWPGC VDLPDYKSIS FAKVESPLGI EGCLPNHSGD VISLLKKLIC
410 420 430 440 450
YDPASRATTM EMLNDKYLSE EPLPVPVSEL YVPPTMSGPD EDSPRKWNDY
460 470
REMDSDSDFD GFGPMNVKPT SSGFTIEFP
Length:479
Mass (Da):53,752
Last modified:November 1, 1998 - v1
Checksum:iCA08BF178B7CC831
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB009399 mRNA. Translation: BAA28775.1.
AL078470 Genomic DNA. Translation: CAB43912.1.
AL161574 Genomic DNA. Translation: CAB79656.1.
CP002687 Genomic DNA. Translation: AEE85567.1.
CP002687 Genomic DNA. Translation: AEE85568.1.
AY042816 mRNA. Translation: AAK68756.1.
AY064627 mRNA. Translation: AAL47340.1.
PIRiT08953.
RefSeqiNP_194627.1. NM_119042.5.
NP_849468.1. NM_179137.3.
UniGeneiAt.19673.

Genome annotation databases

EnsemblPlantsiAT4G28980.1; AT4G28980.1; AT4G28980.
AT4G28980.2; AT4G28980.2; AT4G28980.
GeneIDi829019.
GrameneiAT4G28980.1; AT4G28980.1; AT4G28980.
AT4G28980.2; AT4G28980.2; AT4G28980.
KEGGiath:AT4G28980.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB009399 mRNA. Translation: BAA28775.1.
AL078470 Genomic DNA. Translation: CAB43912.1.
AL161574 Genomic DNA. Translation: CAB79656.1.
CP002687 Genomic DNA. Translation: AEE85567.1.
CP002687 Genomic DNA. Translation: AEE85568.1.
AY042816 mRNA. Translation: AAK68756.1.
AY064627 mRNA. Translation: AAL47340.1.
PIRiT08953.
RefSeqiNP_194627.1. NM_119042.5.
NP_849468.1. NM_179137.3.
UniGeneiAt.19673.

3D structure databases

ProteinModelPortaliO80345.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi14306. 30 interactors.
IntActiO80345. 5 interactors.
STRINGi3702.AT4G28980.1.

PTM databases

iPTMnetiO80345.

Proteomic databases

PaxDbiO80345.
PRIDEiO80345.

Protocols and materials databases

DNASUi829019.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G28980.1; AT4G28980.1; AT4G28980.
AT4G28980.2; AT4G28980.2; AT4G28980.
GeneIDi829019.
GrameneiAT4G28980.1; AT4G28980.1; AT4G28980.
AT4G28980.2; AT4G28980.2; AT4G28980.
KEGGiath:AT4G28980.

Organism-specific databases

TAIRiAT4G28980.

Phylogenomic databases

eggNOGiKOG0594. Eukaryota.
ENOG410XPP3. LUCA.
HOGENOMiHOG000233024.
InParanoidiO80345.
OMAiPGFVAND.
OrthoDBiEOG09360A7L.
PhylomeDBiO80345.

Miscellaneous databases

PROiO80345.

Gene expression databases

ExpressionAtlasiO80345. baseline and differential.
GenevisibleiO80345. AT.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 2 hits.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCDKF1_ARATH
AccessioniPrimary (citable) accession number: O80345
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: November 1, 1998
Last modified: November 30, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.