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Protein

Ethylene-responsive transcription factor 5

Gene

ERF5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Acts as a transcriptional activator. Binds to the GCC-box pathogenesis-related promoter element. Involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi155 – 21359AP2/ERFPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • transcription factor activity, sequence-specific DNA binding Source: TAIR

GO - Biological processi

  • defense response Source: UniProtKB-KW
  • ethylene-activated signaling pathway Source: TAIR
  • response to chitin Source: TAIR
  • response to cold Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Ethylene signaling pathway, Plant defense, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ethylene-responsive transcription factor 5
Short name:
AtERF5
Alternative name(s):
Ethylene-responsive element-binding factor 5
Short name:
EREBP-5
Gene namesi
Name:ERF5
Synonyms:ERF-5, ERF102
Ordered Locus Names:At5g47230
ORF Names:MQL5_9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G47230.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 300300Ethylene-responsive transcription factor 5PRO_0000112563Add
BLAST

Proteomic databases

PaxDbiO80341.
PRIDEiO80341.

Expressioni

Inductioni

Ethylene induction is completely dependent on a functional ETHYLENE-INSENSITIVE2 (EIN2). Wounding as well as cold stress induction does not require EIN2. Transcripts accumulate strongly in cycloheximide-treated plants, a protein synthesis inhibitor. Seems to not be influenced by jasmonate, Alternaria brassicicola, exogenous abscisic acid (ABA), cold, heat, NaCl or drought stress.1 Publication

Gene expression databases

GenevisibleiO80341. AT.

Interactioni

Protein-protein interaction databases

BioGridi20018. 2 interactions.
STRINGi3702.AT5G47230.1.

Structurei

3D structure databases

ProteinModelPortaliO80341.
SMRiO80341. Positions 154-216.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi34 – 5522Ser-richAdd
BLAST

Domaini

The AP2/ERF domain binds specifically to the 5'-GCCGCC-3' motif. The affinity of this binding is higher if the seventh amino-acid of this domain is basic (By similarity).By similarity

Sequence similaritiesi

Contains 1 AP2/ERF DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410JD3F. Eukaryota.
ENOG410Z37D. LUCA.
HOGENOMiHOG000272710.
InParanoidiO80341.
KOiK09286.
OMAiRTRETEN.
PhylomeDBiO80341.

Family and domain databases

Gene3Di3.30.730.10. 1 hit.
InterProiIPR001471. AP2/ERF_dom.
IPR016177. DNA-bd_dom.
[Graphical view]
PfamiPF00847. AP2. 1 hit.
[Graphical view]
PRINTSiPR00367. ETHRSPELEMNT.
SMARTiSM00380. AP2. 1 hit.
[Graphical view]
SUPFAMiSSF54171. SSF54171. 1 hit.
PROSITEiPS51032. AP2_ERF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O80341-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATPNEVSAL WFIEKHLLDE ASPVATDPWM KHESSSATES SSDSSSIIFG
60 70 80 90 100
SSSSSFAPID FSESVCKPEI IDLDTPRSME FLSIPFEFDS EVSVSDFDFK
110 120 130 140 150
PSNQNQNQFE PELKSQIRKP PLKISLPAKT EWIQFAAENT KPEVTKPVSE
160 170 180 190 200
EEKKHYRGVR QRPWGKFAAE IRDPNKRGSR VWLGTFDTAI EAARAYDEAA
210 220 230 240 250
FRLRGSKAIL NFPLEVGKWK PRADEGEKKR KRDDDEKVTV VEKVLKTEQS
260 270 280 290 300
VDVNGGETFP FVTSNLTELC DWDLTGFLNF PLLSPLSPHP PFGYSQLTVV
Length:300
Mass (Da):33,810
Last modified:November 1, 1998 - v1
Checksum:i1189D464A28F7251
GO

Sequence cautioni

The sequence BAA97157.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB008107 mRNA. Translation: BAA32422.1.
AB018117 Genomic DNA. Translation: BAA97157.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED95489.1.
AK117568 mRNA. Translation: BAC42229.1.
AF385709 mRNA. Translation: AAK60301.1.
AY078014 mRNA. Translation: AAL77715.1.
PIRiT52020.
RefSeqiNP_568679.1. NM_124094.2.
UniGeneiAt.9512.

Genome annotation databases

EnsemblPlantsiAT5G47230.1; AT5G47230.1; AT5G47230.
GeneIDi834770.
GrameneiAT5G47230.1; AT5G47230.1; AT5G47230.
KEGGiath:AT5G47230.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB008107 mRNA. Translation: BAA32422.1.
AB018117 Genomic DNA. Translation: BAA97157.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED95489.1.
AK117568 mRNA. Translation: BAC42229.1.
AF385709 mRNA. Translation: AAK60301.1.
AY078014 mRNA. Translation: AAL77715.1.
PIRiT52020.
RefSeqiNP_568679.1. NM_124094.2.
UniGeneiAt.9512.

3D structure databases

ProteinModelPortaliO80341.
SMRiO80341. Positions 154-216.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi20018. 2 interactions.
STRINGi3702.AT5G47230.1.

Proteomic databases

PaxDbiO80341.
PRIDEiO80341.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G47230.1; AT5G47230.1; AT5G47230.
GeneIDi834770.
GrameneiAT5G47230.1; AT5G47230.1; AT5G47230.
KEGGiath:AT5G47230.

Organism-specific databases

TAIRiAT5G47230.

Phylogenomic databases

eggNOGiENOG410JD3F. Eukaryota.
ENOG410Z37D. LUCA.
HOGENOMiHOG000272710.
InParanoidiO80341.
KOiK09286.
OMAiRTRETEN.
PhylomeDBiO80341.

Miscellaneous databases

PROiO80341.

Gene expression databases

GenevisibleiO80341. AT.

Family and domain databases

Gene3Di3.30.730.10. 1 hit.
InterProiIPR001471. AP2/ERF_dom.
IPR016177. DNA-bd_dom.
[Graphical view]
PfamiPF00847. AP2. 1 hit.
[Graphical view]
PRINTSiPR00367. ETHRSPELEMNT.
SMARTiSM00380. AP2. 1 hit.
[Graphical view]
SUPFAMiSSF54171. SSF54171. 1 hit.
PROSITEiPS51032. AP2_ERF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Arabidopsis ethylene responsive element binding factors act as transcriptional activators or repressors of GCC box mediated gene expression."
    Fujimoto S.Y., Ohta M., Usui A., Shinshi H., Ohme-Takagi M.
    Plant Cell 12:393-404(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION.
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
    DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Unique mode of GCC box recognition by the DNA-binding domain of ethylene-responsive element-binding factor (ERF domain) in plant."
    Hao D., Ohme-Takagi M., Sarai A.
    J. Biol. Chem. 273:26857-26861(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Genome-wide analysis of the ERF gene family in Arabidopsis and rice."
    Nakano T., Suzuki K., Fujimura T., Shinshi H.
    Plant Physiol. 140:411-432(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiEF102_ARATH
AccessioniPrimary (citable) accession number: O80341
Secondary accession number(s): Q9LVT6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: February 17, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.