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Protein

Ethylene-responsive transcription factor 4

Gene

ERF4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a transcriptional repressor. Binds to the GCC-box pathogenesis-related promoter element. Involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways, and could also regulate other AtERFs.3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi24 – 81AP2/ERFPROSITE-ProRule annotationAdd BLAST58

GO - Molecular functioni

  • sequence-specific DNA binding Source: TAIR
  • transcription factor activity, sequence-specific DNA binding Source: TAIR

GO - Biological processi

  • ethylene-activated signaling pathway Source: UniProtKB-KW
  • induced systemic resistance, jasmonic acid mediated signaling pathway Source: TAIR
  • negative regulation of ethylene-activated signaling pathway Source: TAIR
  • negative regulation of transcription, DNA-templated Source: TAIR
  • response to abscisic acid Source: TAIR
  • response to chitin Source: TAIR
  • response to ethylene Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Ethylene signaling pathway, Plant defense, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ethylene-responsive transcription factor 4
Short name:
AtERF4
Alternative name(s):
Ethylene-responsive element-binding factor 4
Short name:
EREBP-4
Protein RELATED TO APETALA2 5
Gene namesi
Name:ERF4
Synonyms:ERF-4, ERF078, RAP2-5
Ordered Locus Names:At3g15210
ORF Names:K7L4.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G15210.

Subcellular locationi

GO - Cellular componenti

  • nuclear body Source: TAIR
  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001125541 – 222Ethylene-responsive transcription factor 4Add BLAST222

Proteomic databases

PaxDbiO80340.

Expressioni

Inductioni

Induced by jasmonate (JA) and by Alternaria brassicicola (locally and systemically), but only slightly by ethylene. Strong induction by wounding, cold or drought stress does not require EIN2, whereas induction by NaCl does. Transcripts accumulate in cycloheximide-treated plants, a protein synthesis inhibitor. Seems to not be influenced by exogenous abscisic acid (ABA), and heat stress.2 Publications

Gene expression databases

GenevisibleiO80340. AT.

Interactioni

Subunit structurei

Interacts with SAP18.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
At2g45640O646442EBI-966009,EBI-965964

Protein-protein interaction databases

BioGridi6086. 7 interactors.
IntActiO80340. 1 interactor.
STRINGi3702.AT3G15210.1.

Structurei

3D structure databases

ProteinModelPortaliO80340.
SMRiO80340.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi211 – 217EAR-like (transcriptional repression)7

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi132 – 137Poly-Gly6

Domaini

Contains a slightly degenerated ERF-associated amphiphilic repression (EAR) motif, which may be involved in the activity of transcriptional repression.1 Publication

Sequence similaritiesi

Contains 1 AP2/ERF DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410J0HH. Eukaryota.
ENOG410YQ8J. LUCA.
HOGENOMiHOG000237624.
InParanoidiO80340.
KOiK09286.
OMAiDCASPPT.
OrthoDBiEOG09360Q3E.
PhylomeDBiO80340.

Family and domain databases

CDDicd00018. AP2. 1 hit.
Gene3Di3.30.730.10. 1 hit.
InterProiIPR001471. AP2/ERF_dom.
IPR016177. DNA-bd_dom.
[Graphical view]
PfamiPF00847. AP2. 1 hit.
[Graphical view]
PRINTSiPR00367. ETHRSPELEMNT.
SMARTiSM00380. AP2. 1 hit.
[Graphical view]
SUPFAMiSSF54171. SSF54171. 1 hit.
PROSITEiPS51032. AP2_ERF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O80340-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKMGLKPDP ATTNQTHNNA KEIRYRGVRK RPWGRYAAEI RDPGKKTRVW
60 70 80 90 100
LGTFDTAEEA ARAYDTAARD FRGAKAKTNF PTFLELSDQK VPTGFARSPS
110 120 130 140 150
QSSTLDCASP PTLVVPSATA GNVPPQLELS LGGGGGGSCY QIPMSRPVYF
160 170 180 190 200
LDLMGIGNVG RGQPPPVTSA FRSPVVHVAT KMACGAQSDS DSSSVVDFEG
210 220
GMEKRSQLLD LDLNLPPPSE QA
Length:222
Mass (Da):23,741
Last modified:November 1, 1998 - v1
Checksum:iCEF16ABA5ACFB073
GO

Sequence cautioni

The sequence AAC49771 differs from that shown. Reason: Frameshift at position 208.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF003098 mRNA. Translation: AAC49771.1. Frameshift.
AB008106 mRNA. Translation: BAA32421.1.
AP000413 Genomic DNA. Translation: BAB02150.1.
CP002686 Genomic DNA. Translation: AEE75632.1.
AY140030 mRNA. Translation: AAM98171.1.
BT010325 mRNA. Translation: AAQ55276.1.
AY086232 mRNA. Translation: AAM64308.1.
PIRiT52019.
RefSeqiNP_188139.1. NM_112384.2.
UniGeneiAt.47551.

Genome annotation databases

EnsemblPlantsiAT3G15210.1; AT3G15210.1; AT3G15210.
GeneIDi820752.
GrameneiAT3G15210.1; AT3G15210.1; AT3G15210.
KEGGiath:AT3G15210.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF003098 mRNA. Translation: AAC49771.1. Frameshift.
AB008106 mRNA. Translation: BAA32421.1.
AP000413 Genomic DNA. Translation: BAB02150.1.
CP002686 Genomic DNA. Translation: AEE75632.1.
AY140030 mRNA. Translation: AAM98171.1.
BT010325 mRNA. Translation: AAQ55276.1.
AY086232 mRNA. Translation: AAM64308.1.
PIRiT52019.
RefSeqiNP_188139.1. NM_112384.2.
UniGeneiAt.47551.

3D structure databases

ProteinModelPortaliO80340.
SMRiO80340.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi6086. 7 interactors.
IntActiO80340. 1 interactor.
STRINGi3702.AT3G15210.1.

Proteomic databases

PaxDbiO80340.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G15210.1; AT3G15210.1; AT3G15210.
GeneIDi820752.
GrameneiAT3G15210.1; AT3G15210.1; AT3G15210.
KEGGiath:AT3G15210.

Organism-specific databases

TAIRiAT3G15210.

Phylogenomic databases

eggNOGiENOG410J0HH. Eukaryota.
ENOG410YQ8J. LUCA.
HOGENOMiHOG000237624.
InParanoidiO80340.
KOiK09286.
OMAiDCASPPT.
OrthoDBiEOG09360Q3E.
PhylomeDBiO80340.

Miscellaneous databases

PROiO80340.

Gene expression databases

GenevisibleiO80340. AT.

Family and domain databases

CDDicd00018. AP2. 1 hit.
Gene3Di3.30.730.10. 1 hit.
InterProiIPR001471. AP2/ERF_dom.
IPR016177. DNA-bd_dom.
[Graphical view]
PfamiPF00847. AP2. 1 hit.
[Graphical view]
PRINTSiPR00367. ETHRSPELEMNT.
SMARTiSM00380. AP2. 1 hit.
[Graphical view]
SUPFAMiSSF54171. SSF54171. 1 hit.
PROSITEiPS51032. AP2_ERF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiERF78_ARATH
AccessioniPrimary (citable) accession number: O80340
Secondary accession number(s): O23107, Q53XI2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: November 30, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.