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Protein

Ethylene-responsive transcription factor 3

Gene

ERF3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a transcriptional repressor. Binds to the GCC-box pathogenesis-related promoter element. Involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways and could also regulate other AtERFs.3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi27 – 84AP2/ERFPROSITE-ProRule annotationAdd BLAST58

GO - Molecular functioni

  • sequence-specific DNA binding Source: TAIR
  • transcription factor activity, sequence-specific DNA binding Source: TAIR

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processEthylene signaling pathway, Plant defense, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Ethylene-responsive transcription factor 3
Short name:
AtERF3
Alternative name(s):
Ethylene-responsive element-binding factor 3
Short name:
EREBP-3
Gene namesi
Name:ERF3
Synonyms:ERF-3, ERF082
Ordered Locus Names:At1g50640
ORF Names:F11F12.4, F17J6.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G50640.
TAIRilocus:2008021. AT1G50640.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001125521 – 225Ethylene-responsive transcription factor 3Add BLAST225

Proteomic databases

PaxDbiO80339.

Expressioni

Inductioni

Induced by jasmonate (JA), ethylene and Alternaria brassicicola (locally and systemically). Moderate induction by wounding or drought stress does not require EIN2, whereas induction by NaCl does. Transcripts accumulate slightly in cycloheximide-treated plants, a protein synthesis inhibitor. Seems to not be influenced by ethylene, exogenous abscisic acid (ABA), cold and heat stress.2 Publications

Gene expression databases

ExpressionAtlasiO80339. baseline and differential.
GenevisibleiO80339. AT.

Interactioni

Subunit structurei

Interacts with SAP18.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
At2g45640O646444EBI-965993,EBI-965964

Protein-protein interaction databases

BioGridi26711. 5 interactors.
IntActiO80339. 6 interactors.
STRINGi3702.AT1G50640.1.

Structurei

3D structure databases

ProteinModelPortaliO80339.
SMRiO80339.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi199 – 205EAR-like (transcriptional repression)7

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi90 – 93Poly-Pro4
Compositional biasi181 – 185Poly-Asp5

Domaini

Contains a slightly degenerated ERF-associated amphiphilic repression (EAR) motif, which may be involved in the activity of transcriptional repression.1 Publication

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IZCZ. Eukaryota.
ENOG410YQNQ. LUCA.
HOGENOMiHOG000237624.
InParanoidiO80339.
KOiK09286.
OMAiCDNDNIA.
OrthoDBiEOG09360O9H.
PhylomeDBiO80339.

Family and domain databases

CDDicd00018. AP2. 1 hit.
Gene3Di3.30.730.10. 1 hit.
InterProiView protein in InterPro
IPR001471. AP2/ERF_dom.
IPR036955. AP2/ERF_dom_sf.
IPR016177. DNA-bd_dom.
PfamiView protein in Pfam
PF00847. AP2. 1 hit.
PRINTSiPR00367. ETHRSPELEMNT.
SMARTiView protein in SMART
SM00380. AP2. 1 hit.
SUPFAMiSSF54171. SSF54171. 1 hit.
PROSITEiView protein in PROSITE
PS51032. AP2_ERF. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O80339-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRRGRGSSAV AGPTVVAAIN GSVKEIRFRG VRKRPWGRFA AEIRDPWKKA
60 70 80 90 100
RVWLGTFDSA EEAARAYDSA ARNLRGPKAK TNFPIDSSSP PPPNLRFNQI
110 120 130 140 150
RNQNQNQVDP FMDHRLFTDH QQQFPIVNRP TSSSMSSTVE SFSGPRPTTM
160 170 180 190 200
KPATTKRYPR TPPVVPEDCH SDCDSSSSVI DDDDDIASSS RRRNPPFQFD
210 220
LNFPPLDCVD LFNGADDLHC TDLRL
Length:225
Mass (Da):25,216
Last modified:November 1, 1998 - v1
Checksum:iC709BEABF306776B
GO
Isoform 2 (identifier: O80339-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     2-23: RRGRGSSAVAGPTVVAAINGSV → EKSSCLVRYFRFRRRSCSRLRL
     24-113: Missing.

Note: No experimental confirmation available.
Show »
Length:135
Mass (Da):15,515
Checksum:iC7879F983E9EB0B9
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0235352 – 23RRGRG…INGSV → EKSSCLVRYFRFRRRSCSRL RL in isoform 2. 1 PublicationAdd BLAST22
Alternative sequenceiVSP_02353624 – 113Missing in isoform 2. 1 PublicationAdd BLAST90

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB008105 mRNA. Translation: BAA32420.1.
AC012561 Genomic DNA. Translation: AAF87871.1.
AC079279 Genomic DNA. Translation: AAG51201.1.
CP002684 Genomic DNA. Translation: AEE32574.1.
BT025295 mRNA. Translation: ABF19048.1.
AK228091 mRNA. Translation: BAF00050.1.
PIRiT52011.
RefSeqiNP_175479.1. NM_103946.3. [O80339-1]
UniGeneiAt.1645.
At.71718.

Genome annotation databases

EnsemblPlantsiAT1G50640.1; AT1G50640.1; AT1G50640. [O80339-1]
GeneIDi841486.
GrameneiAT1G50640.1; AT1G50640.1; AT1G50640.
KEGGiath:AT1G50640.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiERF82_ARATH
AccessioniPrimary (citable) accession number: O80339
Secondary accession number(s): Q0WS49, Q1LYT1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: October 25, 2017
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families