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O80339 (ERF82_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ethylene-responsive transcription factor 3

Short name=AtERF3
Alternative name(s):
Ethylene-responsive element-binding factor 3
Short name=EREBP-3
Gene names
Name:ERF3
Synonyms:ERF-3, ERF082
Ordered Locus Names:At1g50640
ORF Names:F11F12.4, F17J6.16
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length225 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a transcriptional repressor. Binds to the GCC-box pathogenesis-related promoter element. Involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways and could also regulate other AtERFs. Ref.1 Ref.6 Ref.7

Subunit structure

Interacts with SAP18. Ref.9

Subcellular location

Nucleus Probable.

Induction

Induced by jasmonate (JA), ethylene and Alternaria brassicicola (locally and systemically). Moderate induction by wounding or drought stress does not require EIN2, whereas induction by NaCl does. Transcripts accumulate slightly in cycloheximide-treated plants, a protein synthesis inhibitor. Seems to not be influenced by ethylene, exogenous abscisic acid (ABA), cold and heat stress. Ref.1 Ref.8

Domain

Contains a slightly degenerated ERF-associated amphiphilic repression (EAR) motif, which may be involved in the activity of transcriptional repression. Ref.7

Sequence similarities

Belongs to the AP2/ERF transcription factor family. ERF subfamily.

Contains 1 AP2/ERF DNA-binding domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

At2g45640O646444EBI-965993,EBI-965964

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O80339-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O80339-2)

The sequence of this isoform differs from the canonical sequence as follows:
     2-23: RRGRGSSAVAGPTVVAAINGSV → EKSSCLVRYFRFRRRSCSRLRL
     24-113: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 225225Ethylene-responsive transcription factor 3
PRO_0000112552

Regions

DNA binding27 – 8458AP2/ERF
Motif199 – 2057EAR-like (transcriptional repression)
Compositional bias90 – 934Poly-Pro
Compositional bias181 – 1855Poly-Asp

Natural variations

Alternative sequence2 – 2322RRGRG…INGSV → EKSSCLVRYFRFRRRSCSRL RL in isoform 2.
VSP_023535
Alternative sequence24 – 11390Missing in isoform 2.
VSP_023536

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: C709BEABF306776B

FASTA22525,216
        10         20         30         40         50         60 
MRRGRGSSAV AGPTVVAAIN GSVKEIRFRG VRKRPWGRFA AEIRDPWKKA RVWLGTFDSA 

        70         80         90        100        110        120 
EEAARAYDSA ARNLRGPKAK TNFPIDSSSP PPPNLRFNQI RNQNQNQVDP FMDHRLFTDH 

       130        140        150        160        170        180 
QQQFPIVNRP TSSSMSSTVE SFSGPRPTTM KPATTKRYPR TPPVVPEDCH SDCDSSSSVI 

       190        200        210        220 
DDDDDIASSS RRRNPPFQFD LNFPPLDCVD LFNGADDLHC TDLRL 

« Hide

Isoform 2 [UniParc].

Checksum: C7879F983E9EB0B9
Show »

FASTA13515,515

References

« Hide 'large scale' references
[1]"Arabidopsis ethylene responsive element binding factors act as transcriptional activators or repressors of GCC box mediated gene expression."
Fujimoto S.Y., Ohta M., Usui A., Shinshi H., Ohme-Takagi M.
Plant Cell 12:393-404(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INDUCTION.
[2]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Arabidopsis ORF clones."
Shinn P., Chen H., Kim C.J., Ecker J.R.
Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[5]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: cv. Columbia.
[6]"Unique mode of GCC box recognition by the DNA-binding domain of ethylene-responsive element-binding factor (ERF domain) in plant."
Hao D., Ohme-Takagi M., Sarai A.
J. Biol. Chem. 273:26857-26861(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Repression domains of class II ERF transcriptional repressors share an essential motif for active repression."
Ohta M., Matsui K., Hiratsu K., Shinshi H., Ohme-Takagi M.
Plant Cell 13:1959-1968(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DOMAIN.
[8]"A role for the GCC-box in jasmonate-mediated activation of the PDF1.2 gene of Arabidopsis."
Brown R.L., Kazan K., McGrath K.C., Maclean D.J., Manners J.M.
Plant Physiol. 132:1020-1032(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[9]"AtSAP18, an orthologue of human SAP18, is involved in the regulation of salt stress and mediates transcriptional repression in Arabidopsis."
Song C.-P., Galbraith D.W.
Plant Mol. Biol. 60:241-257(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SAP18.
[10]"Genome-wide analysis of the ERF gene family in Arabidopsis and rice."
Nakano T., Suzuki K., Fujimura T., Shinshi H.
Plant Physiol. 140:411-432(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB008105 mRNA. Translation: BAA32420.1.
AC012561 Genomic DNA. Translation: AAF87871.1.
AC079279 Genomic DNA. Translation: AAG51201.1.
CP002684 Genomic DNA. Translation: AEE32574.1.
BT025295 mRNA. Translation: ABF19048.1.
AK228091 mRNA. Translation: BAF00050.1.
PIRT52011.
RefSeqNP_175479.1. NM_103946.2. [O80339-1]
UniGeneAt.1645.
At.71718.

3D structure databases

ProteinModelPortalO80339.
SMRO80339. Positions 28-85.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid26711. 4 interactions.
IntActO80339. 2 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G50640.1; AT1G50640.1; AT1G50640. [O80339-1]
GeneID841486.
KEGGath:AT1G50640.

Organism-specific databases

TAIRAT1G50640.

Phylogenomic databases

eggNOGNOG251938.
HOGENOMHOG000237624.
InParanoidO80339.
KOK09286.
OMATKRYPRT.
PhylomeDBO80339.

Gene expression databases

GenevestigatorO80339.

Family and domain databases

Gene3D3.30.730.10. 1 hit.
InterProIPR001471. AP2/ERF_dom.
IPR016177. DNA-bd_dom.
[Graphical view]
PfamPF00847. AP2. 1 hit.
[Graphical view]
PRINTSPR00367. ETHRSPELEMNT.
SMARTSM00380. AP2. 1 hit.
[Graphical view]
SUPFAMSSF54171. SSF54171. 1 hit.
PROSITEPS51032. AP2_ERF. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameERF82_ARATH
AccessionPrimary (citable) accession number: O80339
Secondary accession number(s): Q0WS49, Q1LYT1
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: June 11, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names