O80339 (ERF82_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ethylene-responsive transcription factor 3
Short name=AtERF3

Alternative name(s):
Ethylene-responsive element-binding factor 3
Short name=EREBP-3

Gene names

Name:	ERF3
Synonyms:	ERF-3, ERF082
Ordered Locus Names:	At1g50640
ORF Names:	F11F12.4, F17J6.16

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	225 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a transcriptional repressor. Binds to the GCC-box pathogenesis-related promoter element. Involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways and could also regulate other AtERFs. Ref.1 Ref.6 Ref.7
Subunit structure	Interacts with SAP18. Ref.9
Subcellular location	Nucleus Probable.
Induction	Induced by jasmonate (JA), ethylene and Alternaria brassicicola (locally and systemically). Moderate induction by wounding or drought stress does not require EIN2, whereas induction by NaCl does. Transcripts accumulate slightly in cycloheximide-treated plants, a protein synthesis inhibitor. Seems to not be influenced by ethylene, exogenous abscisic acid (ABA), cold and heat stress. Ref.1 Ref.8
Domain	Contains a slightly degenerated ERF-associated amphiphilic repression (EAR) motif, which may be involved in the activity of transcriptional repression. Ref.7
Sequence similarities	Belongs to the AP2/ERF transcription factor family. ERF subfamily. Contains 1 AP2/ERF DNA-binding domain.

Ontologies

Keywords
Biological process	Ethylene signaling pathway Plant defense Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	DNA-binding
Molecular function	Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	defense response Inferred from electronic annotation. Source: UniProtKB-KW ethylene mediated signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of ethylene mediated signaling pathway Traceable author statement Ref.1. Source: TAIR transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Inferred from sequence or structural similarity PubMed 11118137. Source: TAIR
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
At2g45640	O64644	4	EBI-965993,EBI-965964

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O80339-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O80339-2)

The sequence of this isoform differs from the canonical sequence as follows:
     2-23: RRGRGSSAVAGPTVVAAINGSV → EKSSCLVRYFRFRRRSCSRLRL
     24-113: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 225	225	Ethylene-responsive transcription factor 3		PRO_0000112552
Regions
	DNA binding	27 – 84	58	AP2/ERF
	Motif	199 – 205	7	EAR-like (transcriptional repression)
	Compositional bias	90 – 93	4	Poly-Pro
	Compositional bias	181 – 185	5	Poly-Asp
Natural variations
	Alternative sequence	2 – 23	22	RRGRG…INGSV → EKSSCLVRYFRFRRRSCSRL RL in isoform 2.		VSP_023535
	Alternative sequence	24 – 113	90	Missing in isoform 2.		VSP_023536

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: C709BEABF306776B	FASTA	225	25,216
	10 20 30 40 50 60 MRRGRGSSAV AGPTVVAAIN GSVKEIRFRG VRKRPWGRFA AEIRDPWKKA RVWLGTFDSA 70 80 90 100 110 120 EEAARAYDSA ARNLRGPKAK TNFPIDSSSP PPPNLRFNQI RNQNQNQVDP FMDHRLFTDH 130 140 150 160 170 180 QQQFPIVNRP TSSSMSSTVE SFSGPRPTTM KPATTKRYPR TPPVVPEDCH SDCDSSSSVI 190 200 210 220 DDDDDIASSS RRRNPPFQFD LNFPPLDCVD LFNGADDLHC TDLRL
	Isoform 2 [UniParc]. Checksum: C7879F983E9EB0B9	FASTA	135	15,515
	10 20 30 40 50 60 MEKSSCLVRY FRFRRRSCSR LRLHRLFTDH QQQFPIVNRP TSSSMSSTVE SFSGPRPTTM 70 80 90 100 110 120 KPATTKRYPR TPPVVPEDCH SDCDSSSSVI DDDDDIASSS RRRNPPFQFD LNFPPLDCVD 130 LFNGADDLHC TDLRL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Arabidopsis ethylene responsive element binding factors act as transcriptional activators or repressors of GCC box mediated gene expression." Fujimoto S.Y., Ohta M., Usui A., Shinshi H., Ohme-Takagi M. Plant Cell 12:393-404(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INDUCTION.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Arabidopsis ORF clones." Shinn P., Chen H., Kim C.J., Ecker J.R. Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[5]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: cv. Columbia.
[6]	"Unique mode of GCC box recognition by the DNA-binding domain of ethylene-responsive element-binding factor (ERF domain) in plant." Hao D., Ohme-Takagi M., Sarai A. J. Biol. Chem. 273:26857-26861(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"Repression domains of class II ERF transcriptional repressors share an essential motif for active repression." Ohta M., Matsui K., Hiratsu K., Shinshi H., Ohme-Takagi M. Plant Cell 13:1959-1968(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DOMAIN.
[8]	"A role for the GCC-box in jasmonate-mediated activation of the PDF1.2 gene of Arabidopsis." Brown R.L., Kazan K., McGrath K.C., Maclean D.J., Manners J.M. Plant Physiol. 132:1020-1032(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[9]	"AtSAP18, an orthologue of human SAP18, is involved in the regulation of salt stress and mediates transcriptional repression in Arabidopsis." Song C.-P., Galbraith D.W. Plant Mol. Biol. 60:241-257(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SAP18.
[10]	"Genome-wide analysis of the ERF gene family in Arabidopsis and rice." Nakano T., Suzuki K., Fujimura T., Shinshi H. Plant Physiol. 140:411-432(2006) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB008105 mRNA. Translation: BAA32420.1. AC012561 Genomic DNA. Translation: AAF87871.1. AC079279 Genomic DNA. Translation: AAG51201.1. CP002684 Genomic DNA. Translation: AEE32574.1. BT025295 mRNA. Translation: ABF19048.1. AK228091 mRNA. Translation: BAF00050.1.
IPI	IPI00534661. IPI00837459.
PIR	T52011.
RefSeq	NP_175479.1. NM_103946.2.
UniGene	At.1645. At.71718.
3D structure databases
ProteinModelPortal	O80339.
SMR	O80339. Positions 28-85.
ModBase	Search...
Protein-protein interaction databases
IntAct	O80339. 2 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G50640.1; AT1G50640.1; AT1G50640.
GeneID	841486.
KEGG	ath:AT1G50640.
Organism-specific databases
TAIR	At1g50640.
Phylogenomic databases
eggNOG	NOG251938.
HOGENOM	HOG000237624.
InParanoid	O80339.
KO	K09286.
OMA	TKRYPRT.
PhylomeDB	O80339.
ProtClustDB	CLSN2682907.
Gene expression databases
ArrayExpress	O80339.
Genevestigator	O80339.
GermOnline	AT1G50640. Arabidopsis thaliana.
Family and domain databases
Gene3D	3.30.730.10. 1 hit.
InterPro	IPR001471. AP2/ERF_dom. IPR016177. DNA-bd_integrase-typ. [Graphical view]
Pfam	PF00847. AP2. 1 hit. [Graphical view]
PRINTS	PR00367. ETHRSPELEMNT.
SMART	SM00380. AP2. 1 hit. [Graphical view]
SUPFAM	SSF54171. DNA-binding_integrase-type. 1 hit.
PROSITE	PS51032. AP2_ERF. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ERF82_ARATH

Accession

Primary (citable) accession number: O80339
Secondary accession number(s): Q0WS49, Q1LYT1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1998
Last modified:	May 1, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program