Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ethylene-responsive transcription factor 3

Gene

ERF3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a transcriptional repressor. Binds to the GCC-box pathogenesis-related promoter element. Involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways and could also regulate other AtERFs.3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi27 – 8458AP2/ERFPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. sequence-specific DNA binding transcription factor activity Source: TAIR

GO - Biological processi

  1. defense response Source: UniProtKB-KW
  2. ethylene-activated signaling pathway Source: UniProtKB-KW
  3. negative regulation of ethylene-activated signaling pathway Source: TAIR
  4. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Ethylene signaling pathway, Plant defense, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ethylene-responsive transcription factor 3
Short name:
AtERF3
Alternative name(s):
Ethylene-responsive element-binding factor 3
Short name:
EREBP-3
Gene namesi
Name:ERF3
Synonyms:ERF-3, ERF082
Ordered Locus Names:At1g50640
ORF Names:F11F12.4, F17J6.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G50640.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 225225Ethylene-responsive transcription factor 3PRO_0000112552Add
BLAST

Expressioni

Inductioni

Induced by jasmonate (JA), ethylene and Alternaria brassicicola (locally and systemically). Moderate induction by wounding or drought stress does not require EIN2, whereas induction by NaCl does. Transcripts accumulate slightly in cycloheximide-treated plants, a protein synthesis inhibitor. Seems to not be influenced by ethylene, exogenous abscisic acid (ABA), cold and heat stress.2 Publications

Gene expression databases

ExpressionAtlasiO80339. baseline and differential.
GenevestigatoriO80339.

Interactioni

Subunit structurei

Interacts with SAP18.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
At2g45640O646444EBI-965993,EBI-965964

Protein-protein interaction databases

BioGridi26711. 4 interactions.
IntActiO80339. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliO80339.
SMRiO80339. Positions 28-85.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi199 – 2057EAR-like (transcriptional repression)

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi90 – 934Poly-Pro
Compositional biasi181 – 1855Poly-Asp

Domaini

Contains a slightly degenerated ERF-associated amphiphilic repression (EAR) motif, which may be involved in the activity of transcriptional repression.1 Publication

Sequence similaritiesi

Contains 1 AP2/ERF DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG251938.
HOGENOMiHOG000237624.
InParanoidiO80339.
KOiK09286.
OMAiDLHCTAL.
PhylomeDBiO80339.

Family and domain databases

Gene3Di3.30.730.10. 1 hit.
InterProiIPR001471. AP2/ERF_dom.
IPR016177. DNA-bd_dom.
[Graphical view]
PfamiPF00847. AP2. 1 hit.
[Graphical view]
PRINTSiPR00367. ETHRSPELEMNT.
SMARTiSM00380. AP2. 1 hit.
[Graphical view]
SUPFAMiSSF54171. SSF54171. 1 hit.
PROSITEiPS51032. AP2_ERF. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O80339-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRRGRGSSAV AGPTVVAAIN GSVKEIRFRG VRKRPWGRFA AEIRDPWKKA
60 70 80 90 100
RVWLGTFDSA EEAARAYDSA ARNLRGPKAK TNFPIDSSSP PPPNLRFNQI
110 120 130 140 150
RNQNQNQVDP FMDHRLFTDH QQQFPIVNRP TSSSMSSTVE SFSGPRPTTM
160 170 180 190 200
KPATTKRYPR TPPVVPEDCH SDCDSSSSVI DDDDDIASSS RRRNPPFQFD
210 220
LNFPPLDCVD LFNGADDLHC TDLRL
Length:225
Mass (Da):25,216
Last modified:November 1, 1998 - v1
Checksum:iC709BEABF306776B
GO
Isoform 2 (identifier: O80339-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     2-23: RRGRGSSAVAGPTVVAAINGSV → EKSSCLVRYFRFRRRSCSRLRL
     24-113: Missing.

Note: No experimental confirmation available.

Show »
Length:135
Mass (Da):15,515
Checksum:iC7879F983E9EB0B9
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei2 – 2322RRGRG…INGSV → EKSSCLVRYFRFRRRSCSRL RL in isoform 2. 1 PublicationVSP_023535Add
BLAST
Alternative sequencei24 – 11390Missing in isoform 2. 1 PublicationVSP_023536Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB008105 mRNA. Translation: BAA32420.1.
AC012561 Genomic DNA. Translation: AAF87871.1.
AC079279 Genomic DNA. Translation: AAG51201.1.
CP002684 Genomic DNA. Translation: AEE32574.1.
BT025295 mRNA. Translation: ABF19048.1.
AK228091 mRNA. Translation: BAF00050.1.
PIRiT52011.
RefSeqiNP_175479.1. NM_103946.2. [O80339-1]
UniGeneiAt.1645.
At.71718.

Genome annotation databases

EnsemblPlantsiAT1G50640.1; AT1G50640.1; AT1G50640. [O80339-1]
GeneIDi841486.
KEGGiath:AT1G50640.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB008105 mRNA. Translation: BAA32420.1.
AC012561 Genomic DNA. Translation: AAF87871.1.
AC079279 Genomic DNA. Translation: AAG51201.1.
CP002684 Genomic DNA. Translation: AEE32574.1.
BT025295 mRNA. Translation: ABF19048.1.
AK228091 mRNA. Translation: BAF00050.1.
PIRiT52011.
RefSeqiNP_175479.1. NM_103946.2. [O80339-1]
UniGeneiAt.1645.
At.71718.

3D structure databases

ProteinModelPortaliO80339.
SMRiO80339. Positions 28-85.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi26711. 4 interactions.
IntActiO80339. 2 interactions.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G50640.1; AT1G50640.1; AT1G50640. [O80339-1]
GeneIDi841486.
KEGGiath:AT1G50640.

Organism-specific databases

TAIRiAT1G50640.

Phylogenomic databases

eggNOGiNOG251938.
HOGENOMiHOG000237624.
InParanoidiO80339.
KOiK09286.
OMAiDLHCTAL.
PhylomeDBiO80339.

Gene expression databases

ExpressionAtlasiO80339. baseline and differential.
GenevestigatoriO80339.

Family and domain databases

Gene3Di3.30.730.10. 1 hit.
InterProiIPR001471. AP2/ERF_dom.
IPR016177. DNA-bd_dom.
[Graphical view]
PfamiPF00847. AP2. 1 hit.
[Graphical view]
PRINTSiPR00367. ETHRSPELEMNT.
SMARTiSM00380. AP2. 1 hit.
[Graphical view]
SUPFAMiSSF54171. SSF54171. 1 hit.
PROSITEiPS51032. AP2_ERF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Arabidopsis ethylene responsive element binding factors act as transcriptional activators or repressors of GCC box mediated gene expression."
    Fujimoto S.Y., Ohta M., Usui A., Shinshi H., Ohme-Takagi M.
    Plant Cell 12:393-404(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INDUCTION.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Arabidopsis ORF clones."
    Shinn P., Chen H., Kim C.J., Ecker J.R.
    Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Columbia.
  6. "Unique mode of GCC box recognition by the DNA-binding domain of ethylene-responsive element-binding factor (ERF domain) in plant."
    Hao D., Ohme-Takagi M., Sarai A.
    J. Biol. Chem. 273:26857-26861(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Repression domains of class II ERF transcriptional repressors share an essential motif for active repression."
    Ohta M., Matsui K., Hiratsu K., Shinshi H., Ohme-Takagi M.
    Plant Cell 13:1959-1968(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DOMAIN.
  8. "A role for the GCC-box in jasmonate-mediated activation of the PDF1.2 gene of Arabidopsis."
    Brown R.L., Kazan K., McGrath K.C., Maclean D.J., Manners J.M.
    Plant Physiol. 132:1020-1032(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  9. "AtSAP18, an orthologue of human SAP18, is involved in the regulation of salt stress and mediates transcriptional repression in Arabidopsis."
    Song C.-P., Galbraith D.W.
    Plant Mol. Biol. 60:241-257(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SAP18.
  10. "Genome-wide analysis of the ERF gene family in Arabidopsis and rice."
    Nakano T., Suzuki K., Fujimura T., Shinshi H.
    Plant Physiol. 140:411-432(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiERF82_ARATH
AccessioniPrimary (citable) accession number: O80339
Secondary accession number(s): Q0WS49, Q1LYT1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: January 7, 2015
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.