Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O80339

- ERF82_ARATH

UniProt

O80339 - ERF82_ARATH

Protein

Ethylene-responsive transcription factor 3

Gene

ERF3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Acts as a transcriptional repressor. Binds to the GCC-box pathogenesis-related promoter element. Involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways and could also regulate other AtERFs.3 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi27 – 8458AP2/ERFPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. protein binding Source: IntAct
    3. sequence-specific DNA binding transcription factor activity Source: TAIR

    GO - Biological processi

    1. defense response Source: UniProtKB-KW
    2. ethylene-activated signaling pathway Source: UniProtKB-KW
    3. negative regulation of ethylene-activated signaling pathway Source: TAIR
    4. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Repressor

    Keywords - Biological processi

    Ethylene signaling pathway, Plant defense, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ethylene-responsive transcription factor 3
    Short name:
    AtERF3
    Alternative name(s):
    Ethylene-responsive element-binding factor 3
    Short name:
    EREBP-3
    Gene namesi
    Name:ERF3
    Synonyms:ERF-3, ERF082
    Ordered Locus Names:At1g50640
    ORF Names:F11F12.4, F17J6.16
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G50640.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 225225Ethylene-responsive transcription factor 3PRO_0000112552Add
    BLAST

    Expressioni

    Inductioni

    Induced by jasmonate (JA), ethylene and Alternaria brassicicola (locally and systemically). Moderate induction by wounding or drought stress does not require EIN2, whereas induction by NaCl does. Transcripts accumulate slightly in cycloheximide-treated plants, a protein synthesis inhibitor. Seems to not be influenced by ethylene, exogenous abscisic acid (ABA), cold and heat stress.2 Publications

    Gene expression databases

    GenevestigatoriO80339.

    Interactioni

    Subunit structurei

    Interacts with SAP18.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    At2g45640O646444EBI-965993,EBI-965964

    Protein-protein interaction databases

    BioGridi26711. 4 interactions.
    IntActiO80339. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliO80339.
    SMRiO80339. Positions 28-85.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi199 – 2057EAR-like (transcriptional repression)

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi90 – 934Poly-Pro
    Compositional biasi181 – 1855Poly-Asp

    Domaini

    Contains a slightly degenerated ERF-associated amphiphilic repression (EAR) motif, which may be involved in the activity of transcriptional repression.1 Publication

    Sequence similaritiesi

    Contains 1 AP2/ERF DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG251938.
    HOGENOMiHOG000237624.
    InParanoidiO80339.
    KOiK09286.
    OMAiTKRYPRT.
    PhylomeDBiO80339.

    Family and domain databases

    Gene3Di3.30.730.10. 1 hit.
    InterProiIPR001471. AP2/ERF_dom.
    IPR016177. DNA-bd_dom.
    [Graphical view]
    PfamiPF00847. AP2. 1 hit.
    [Graphical view]
    PRINTSiPR00367. ETHRSPELEMNT.
    SMARTiSM00380. AP2. 1 hit.
    [Graphical view]
    SUPFAMiSSF54171. SSF54171. 1 hit.
    PROSITEiPS51032. AP2_ERF. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O80339-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRRGRGSSAV AGPTVVAAIN GSVKEIRFRG VRKRPWGRFA AEIRDPWKKA    50
    RVWLGTFDSA EEAARAYDSA ARNLRGPKAK TNFPIDSSSP PPPNLRFNQI 100
    RNQNQNQVDP FMDHRLFTDH QQQFPIVNRP TSSSMSSTVE SFSGPRPTTM 150
    KPATTKRYPR TPPVVPEDCH SDCDSSSSVI DDDDDIASSS RRRNPPFQFD 200
    LNFPPLDCVD LFNGADDLHC TDLRL 225
    Length:225
    Mass (Da):25,216
    Last modified:November 1, 1998 - v1
    Checksum:iC709BEABF306776B
    GO
    Isoform 2 (identifier: O80339-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         2-23: RRGRGSSAVAGPTVVAAINGSV → EKSSCLVRYFRFRRRSCSRLRL
         24-113: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:135
    Mass (Da):15,515
    Checksum:iC7879F983E9EB0B9
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei2 – 2322RRGRG…INGSV → EKSSCLVRYFRFRRRSCSRL RL in isoform 2. 1 PublicationVSP_023535Add
    BLAST
    Alternative sequencei24 – 11390Missing in isoform 2. 1 PublicationVSP_023536Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB008105 mRNA. Translation: BAA32420.1.
    AC012561 Genomic DNA. Translation: AAF87871.1.
    AC079279 Genomic DNA. Translation: AAG51201.1.
    CP002684 Genomic DNA. Translation: AEE32574.1.
    BT025295 mRNA. Translation: ABF19048.1.
    AK228091 mRNA. Translation: BAF00050.1.
    PIRiT52011.
    RefSeqiNP_175479.1. NM_103946.2. [O80339-1]
    UniGeneiAt.1645.
    At.71718.

    Genome annotation databases

    EnsemblPlantsiAT1G50640.1; AT1G50640.1; AT1G50640. [O80339-1]
    GeneIDi841486.
    KEGGiath:AT1G50640.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB008105 mRNA. Translation: BAA32420.1 .
    AC012561 Genomic DNA. Translation: AAF87871.1 .
    AC079279 Genomic DNA. Translation: AAG51201.1 .
    CP002684 Genomic DNA. Translation: AEE32574.1 .
    BT025295 mRNA. Translation: ABF19048.1 .
    AK228091 mRNA. Translation: BAF00050.1 .
    PIRi T52011.
    RefSeqi NP_175479.1. NM_103946.2. [O80339-1 ]
    UniGenei At.1645.
    At.71718.

    3D structure databases

    ProteinModelPortali O80339.
    SMRi O80339. Positions 28-85.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 26711. 4 interactions.
    IntActi O80339. 2 interactions.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G50640.1 ; AT1G50640.1 ; AT1G50640 . [O80339-1 ]
    GeneIDi 841486.
    KEGGi ath:AT1G50640.

    Organism-specific databases

    TAIRi AT1G50640.

    Phylogenomic databases

    eggNOGi NOG251938.
    HOGENOMi HOG000237624.
    InParanoidi O80339.
    KOi K09286.
    OMAi TKRYPRT.
    PhylomeDBi O80339.

    Gene expression databases

    Genevestigatori O80339.

    Family and domain databases

    Gene3Di 3.30.730.10. 1 hit.
    InterProi IPR001471. AP2/ERF_dom.
    IPR016177. DNA-bd_dom.
    [Graphical view ]
    Pfami PF00847. AP2. 1 hit.
    [Graphical view ]
    PRINTSi PR00367. ETHRSPELEMNT.
    SMARTi SM00380. AP2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54171. SSF54171. 1 hit.
    PROSITEi PS51032. AP2_ERF. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Arabidopsis ethylene responsive element binding factors act as transcriptional activators or repressors of GCC box mediated gene expression."
      Fujimoto S.Y., Ohta M., Usui A., Shinshi H., Ohme-Takagi M.
      Plant Cell 12:393-404(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INDUCTION.
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Arabidopsis ORF clones."
      Shinn P., Chen H., Kim C.J., Ecker J.R.
      Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: cv. Columbia.
    6. "Unique mode of GCC box recognition by the DNA-binding domain of ethylene-responsive element-binding factor (ERF domain) in plant."
      Hao D., Ohme-Takagi M., Sarai A.
      J. Biol. Chem. 273:26857-26861(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Repression domains of class II ERF transcriptional repressors share an essential motif for active repression."
      Ohta M., Matsui K., Hiratsu K., Shinshi H., Ohme-Takagi M.
      Plant Cell 13:1959-1968(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DOMAIN.
    8. "A role for the GCC-box in jasmonate-mediated activation of the PDF1.2 gene of Arabidopsis."
      Brown R.L., Kazan K., McGrath K.C., Maclean D.J., Manners J.M.
      Plant Physiol. 132:1020-1032(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    9. "AtSAP18, an orthologue of human SAP18, is involved in the regulation of salt stress and mediates transcriptional repression in Arabidopsis."
      Song C.-P., Galbraith D.W.
      Plant Mol. Biol. 60:241-257(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SAP18.
    10. "Genome-wide analysis of the ERF gene family in Arabidopsis and rice."
      Nakano T., Suzuki K., Fujimura T., Shinshi H.
      Plant Physiol. 140:411-432(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiERF82_ARATH
    AccessioniPrimary (citable) accession number: O80339
    Secondary accession number(s): Q0WS49, Q1LYT1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 118 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3