Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O80337

- EF100_ARATH

UniProt

O80337 - EF100_ARATH

Protein

Ethylene-responsive transcription factor 1A

Gene

ERF1A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 2 (27 May 2002)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Acts as a transcriptional activator. Binds to the GCC-box pathogenesis-related promoter element. Involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways.3 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi147 – 20559AP2/ERFPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. sequence-specific DNA binding transcription factor activity Source: TAIR

    GO - Biological processi

    1. defense response Source: UniProtKB-KW
    2. ethylene-activated signaling pathway Source: UniProtKB-KW
    3. response to chitin Source: TAIR
    4. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Ethylene signaling pathway, Plant defense, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ethylene-responsive transcription factor 1A
    Short name:
    AtERF1A
    Alternative name(s):
    Ethylene-responsive element-binding factor 1A
    Short name:
    EREBP-1A
    Gene namesi
    Name:ERF1A
    Synonyms:ERF-1, ERF100
    Ordered Locus Names:At4g17500
    ORF Names:dl4785w, FCAALL.123
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 4

    Organism-specific databases

    TAIRiAT4G17500.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 268268Ethylene-responsive transcription factor 1APRO_0000112542Add
    BLAST

    Proteomic databases

    PRIDEiO80337.

    Expressioni

    Tissue specificityi

    Ubiquitously expressed, mostly in flowers and rosettes after ethylene treatment.1 Publication

    Inductioni

    Induced by Pseudomonas syringae tomato (both virulent and avirulent avrRpt2 strains), independently of PAD4. Also induced by methyl jasmonate (MeJA) independently of JAR1. Ethylene induction is completely dependent on a functional ETHYLENE-INSENSITIVE2 (EIN2), whereas induction by wounding does not need EIN2. Induction by salicylic acid (SA) seems to be independent of PAD4 and NPR1. Transcripts accumulate strongly in cycloheximide-treated plants, a protein synthesis inhibitor. Seems to not be influenced by exogenous abscisic acid (ABA), cold, heat, NaCl or drought stress.2 Publications

    Gene expression databases

    GenevestigatoriO80337.

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-8070094.

    Structurei

    Secondary structure

    1
    268
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi151 – 1544
    Turni155 – 1573
    Beta strandi158 – 1658
    Turni166 – 1694
    Beta strandi170 – 18011
    Helixi181 – 19616
    Beta strandi203 – 2064

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1GCCNMR-A146-208[»]
    2GCCNMR-A143-212[»]
    3GCCNMR-A143-212[»]
    ProteinModelPortaliO80337.
    SMRiO80337. Positions 146-208.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO80337.

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi221 – 23717Nuclear localization signalSequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi98 – 1025Poly-Ser

    Domaini

    The AP2/ERF domain binds specifically to the 5'-GCCGCC-3' motif. The affinity of this binding is higher if the seventh amino-acid of this domain is basic By similarity.By similarity

    Sequence similaritiesi

    Contains 1 AP2/ERF DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG246525.
    HOGENOMiHOG000239314.
    InParanoidiO80337.
    KOiK09286.
    OMAiHNDSCRR.
    PhylomeDBiO80337.

    Family and domain databases

    Gene3Di3.30.730.10. 1 hit.
    InterProiIPR001471. AP2/ERF_dom.
    IPR016177. DNA-bd_dom.
    [Graphical view]
    PfamiPF00847. AP2. 1 hit.
    [Graphical view]
    PRINTSiPR00367. ETHRSPELEMNT.
    SMARTiSM00380. AP2. 1 hit.
    [Graphical view]
    SUPFAMiSSF54171. SSF54171. 1 hit.
    PROSITEiPS51032. AP2_ERF. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O80337-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSMTADSQSD YAFLESIRRH LLGESEPILS ESTASSVTQS CVTGQSIKPV    50
    YGRNPSFSKL YPCFTESWGD LPLKENDSED MLVYGILNDA FHGGWEPSSS 100
    SSDEDRSSFP SVKIETPESF AAVDSVPVKK EKTSPVSAAV TAAKGKHYRG 150
    VRQRPWGKFA AEIRDPAKNG ARVWLGTFET AEDAALAYDR AAFRMRGSRA 200
    LLNFPLRVNS GEPDPVRIKS KRSSFSSSNE NGAPKKRRTV AAGGGMDKGL 250
    TVKCEVVEVA RGDRLLVL 268
    Length:268
    Mass (Da):29,190
    Last modified:May 27, 2002 - v2
    Checksum:i0FB7839984E97B50
    GO

    Sequence cautioni

    The sequence CAB45963.1 differs from that shown. Reason: Frameshift at position 44.
    The sequence CAB78753.1 differs from that shown. Reason: Frameshift at position 44.
    The sequence BAA32418.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z97343 Genomic DNA. Translation: CAB45963.1. Frameshift.
    AL161546 Genomic DNA. Translation: CAB78753.1. Frameshift.
    CP002687 Genomic DNA. Translation: AEE83903.1.
    AY058174 mRNA. Translation: AAL25588.1.
    AY062533 mRNA. Translation: AAL32611.1.
    BT002578 mRNA. Translation: AAO00938.1.
    AB008103 mRNA. Translation: BAA32418.1. Different initiation.
    PIRiA85196.
    T51988.
    RefSeqiNP_567530.4. NM_117855.5.
    UniGeneiAt.23185.
    At.68141.

    Genome annotation databases

    EnsemblPlantsiAT4G17500.1; AT4G17500.1; AT4G17500.
    GeneIDi827464.
    KEGGiath:AT4G17500.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z97343 Genomic DNA. Translation: CAB45963.1 . Frameshift.
    AL161546 Genomic DNA. Translation: CAB78753.1 . Frameshift.
    CP002687 Genomic DNA. Translation: AEE83903.1 .
    AY058174 mRNA. Translation: AAL25588.1 .
    AY062533 mRNA. Translation: AAL32611.1 .
    BT002578 mRNA. Translation: AAO00938.1 .
    AB008103 mRNA. Translation: BAA32418.1 . Different initiation.
    PIRi A85196.
    T51988.
    RefSeqi NP_567530.4. NM_117855.5.
    UniGenei At.23185.
    At.68141.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1GCC NMR - A 146-208 [» ]
    2GCC NMR - A 143-212 [» ]
    3GCC NMR - A 143-212 [» ]
    ProteinModelPortali O80337.
    SMRi O80337. Positions 146-208.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-8070094.

    Proteomic databases

    PRIDEi O80337.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT4G17500.1 ; AT4G17500.1 ; AT4G17500 .
    GeneIDi 827464.
    KEGGi ath:AT4G17500.

    Organism-specific databases

    TAIRi AT4G17500.

    Phylogenomic databases

    eggNOGi NOG246525.
    HOGENOMi HOG000239314.
    InParanoidi O80337.
    KOi K09286.
    OMAi HNDSCRR.
    PhylomeDBi O80337.

    Miscellaneous databases

    EvolutionaryTracei O80337.

    Gene expression databases

    Genevestigatori O80337.

    Family and domain databases

    Gene3Di 3.30.730.10. 1 hit.
    InterProi IPR001471. AP2/ERF_dom.
    IPR016177. DNA-bd_dom.
    [Graphical view ]
    Pfami PF00847. AP2. 1 hit.
    [Graphical view ]
    PRINTSi PR00367. ETHRSPELEMNT.
    SMARTi SM00380. AP2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54171. SSF54171. 1 hit.
    PROSITEi PS51032. AP2_ERF. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana."
      Bevan M., Bancroft I., Bent E., Love K., Goodman H.M., Dean C., Bergkamp R., Dirkse W., van Staveren M., Stiekema W., Drost L., Ridley P., Hudson S.-A., Patel K., Murphy G., Piffanelli P., Wedler H., Wedler E.
      , Wambutt R., Weitzenegger T., Pohl T., Terryn N., Gielen J., Villarroel R., De Clercq R., van Montagu M., Lecharny A., Aubourg S., Gy I., Kreis M., Lao N., Kavanagh T., Hempel S., Kotter P., Entian K.-D., Rieger M., Schaefer M., Funk B., Mueller-Auer S., Silvey M., James R., Monfort A., Pons A., Puigdomenech P., Douka A., Voukelatou E., Milioni D., Hatzopoulos P., Piravandi E., Obermaier B., Hilbert H., Duesterhoeft A., Moores T., Jones J.D.G., Eneva T., Palme K., Benes V., Rechmann S., Ansorge W., Cooke R., Berger C., Delseny M., Voet M., Volckaert G., Mewes H.-W., Klosterman S., Schueller C., Chalwatzis N.
      Nature 391:485-488(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
      Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
      , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
      Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Arabidopsis ethylene responsive element binding factors act as transcriptional activators or repressors of GCC box mediated gene expression."
      Fujimoto S.Y., Ohta M., Usui A., Shinshi H., Ohme-Takagi M.
      Plant Cell 12:393-404(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-268, FUNCTION, INDUCTION.
      Strain: cv. C24.
    6. "Unique mode of GCC box recognition by the DNA-binding domain of ethylene-responsive element-binding factor (ERF domain) in plant."
      Hao D., Ohme-Takagi M., Sarai A.
      J. Biol. Chem. 273:26857-26861(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Identification of Arabidopsis ethylene-responsive element binding factors with distinct induction kinetics after pathogen infection."
      Onate-Sanchez L., Singh K.B.
      Plant Physiol. 128:1313-1322(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, INDUCTION.
    8. "A novel mode of DNA recognition by a beta-sheet revealed by the solution structure of the GCC-box binding domain in complex with DNA."
      Allen M.D., Yamasaki K., Ohme-Takagi M., Tateno M., Suzuki M.
      EMBO J. 17:5484-5496(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 146-208.
    9. "Genome-wide analysis of the ERF gene family in Arabidopsis and rice."
      Nakano T., Suzuki K., Fujimura T., Shinshi H.
      Plant Physiol. 140:411-432(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiEF100_ARATH
    AccessioniPrimary (citable) accession number: O80337
    Secondary accession number(s): Q93Z36, Q9SUK1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: May 27, 2002
    Last modified: October 1, 2014
    This is version 127 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Caution

    Two different genes At3g23240 and At4g17500 were assigned the same gene name ERF1.Curated
    It is uncertain whether Met-1 or Met-3 is the initiator.Curated

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3