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Protein

Ethylene-responsive transcription factor 1A

Gene

ERF1A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a transcriptional activator. Binds to the GCC-box pathogenesis-related promoter element. Involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways.3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi147 – 205AP2/ERFPROSITE-ProRule annotationAdd BLAST59

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • transcription factor activity, sequence-specific DNA binding Source: TAIR

GO - Biological processi

  • cell division Source: TAIR
  • defense response Source: UniProtKB-KW
  • ethylene-activated signaling pathway Source: UniProtKB-KW
  • response to chitin Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW
  • vasculature development Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Ethylene signaling pathway, Plant defense, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ethylene-responsive transcription factor 1A
Short name:
AtERF1A
Alternative name(s):
Ethylene-responsive element-binding factor 1A
Short name:
EREBP-1A
Gene namesi
Name:ERF1A
Synonyms:ERF-1, ERF100
Ordered Locus Names:At4g17500
ORF Names:dl4785w, FCAALL.123
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G17500.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001125421 – 268Ethylene-responsive transcription factor 1AAdd BLAST268

Proteomic databases

PaxDbiO80337.

Expressioni

Tissue specificityi

Ubiquitously expressed, mostly in flowers and rosettes after ethylene treatment.1 Publication

Inductioni

Induced by Pseudomonas syringae tomato (both virulent and avirulent avrRpt2 strains), independently of PAD4. Also induced by methyl jasmonate (MeJA) independently of JAR1. Ethylene induction is completely dependent on a functional ETHYLENE-INSENSITIVE2 (EIN2), whereas induction by wounding does not need EIN2. Induction by salicylic acid (SA) seems to be independent of PAD4 and NPR1. Transcripts accumulate strongly in cycloheximide-treated plants, a protein synthesis inhibitor. Seems to not be influenced by exogenous abscisic acid (ABA), cold, heat, NaCl or drought stress.2 Publications

Gene expression databases

GenevisibleiO80337. AT.

Interactioni

Protein-protein interaction databases

BioGridi12757. 1 interactor.
MINTiMINT-8070094.
STRINGi3702.AT4G17500.1.

Structurei

Secondary structure

1268
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi151 – 154Combined sources4
Turni155 – 157Combined sources3
Beta strandi158 – 165Combined sources8
Turni166 – 169Combined sources4
Beta strandi170 – 180Combined sources11
Helixi181 – 196Combined sources16
Beta strandi203 – 206Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GCCNMR-A146-208[»]
2GCCNMR-A143-212[»]
3GCCNMR-A143-212[»]
ProteinModelPortaliO80337.
SMRiO80337.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO80337.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi221 – 237Nuclear localization signalSequence analysisAdd BLAST17

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi98 – 102Poly-Ser5

Domaini

The AP2/ERF domain binds specifically to the 5'-GCCGCC-3' motif. The affinity of this binding is higher if the seventh amino-acid of this domain is basic (By similarity).By similarity

Sequence similaritiesi

Contains 1 AP2/ERF DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410J07P. Eukaryota.
ENOG410YJV7. LUCA.
HOGENOMiHOG000239314.
InParanoidiO80337.
KOiK09286.
OMAiCEVVEVT.
OrthoDBiEOG09360R86.
PhylomeDBiO80337.

Family and domain databases

CDDicd00018. AP2. 1 hit.
Gene3Di3.30.730.10. 1 hit.
InterProiIPR001471. AP2/ERF_dom.
IPR016177. DNA-bd_dom.
[Graphical view]
PfamiPF00847. AP2. 1 hit.
[Graphical view]
PRINTSiPR00367. ETHRSPELEMNT.
SMARTiSM00380. AP2. 1 hit.
[Graphical view]
SUPFAMiSSF54171. SSF54171. 1 hit.
PROSITEiPS51032. AP2_ERF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O80337-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSMTADSQSD YAFLESIRRH LLGESEPILS ESTASSVTQS CVTGQSIKPV
60 70 80 90 100
YGRNPSFSKL YPCFTESWGD LPLKENDSED MLVYGILNDA FHGGWEPSSS
110 120 130 140 150
SSDEDRSSFP SVKIETPESF AAVDSVPVKK EKTSPVSAAV TAAKGKHYRG
160 170 180 190 200
VRQRPWGKFA AEIRDPAKNG ARVWLGTFET AEDAALAYDR AAFRMRGSRA
210 220 230 240 250
LLNFPLRVNS GEPDPVRIKS KRSSFSSSNE NGAPKKRRTV AAGGGMDKGL
260
TVKCEVVEVA RGDRLLVL
Length:268
Mass (Da):29,190
Last modified:May 27, 2002 - v2
Checksum:i0FB7839984E97B50
GO

Sequence cautioni

The sequence BAA32418 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAB45963 differs from that shown. Reason: Frameshift at position 44.Curated
The sequence CAB78753 differs from that shown. Reason: Frameshift at position 44.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z97343 Genomic DNA. Translation: CAB45963.1. Frameshift.
AL161546 Genomic DNA. Translation: CAB78753.1. Frameshift.
CP002687 Genomic DNA. Translation: AEE83903.1.
AY058174 mRNA. Translation: AAL25588.1.
AY062533 mRNA. Translation: AAL32611.1.
BT002578 mRNA. Translation: AAO00938.1.
AB008103 mRNA. Translation: BAA32418.1. Different initiation.
PIRiA85196.
T51988.
RefSeqiNP_567530.4. NM_117855.6.
UniGeneiAt.23185.
At.68141.

Genome annotation databases

EnsemblPlantsiAT4G17500.1; AT4G17500.1; AT4G17500.
GeneIDi827464.
GrameneiAT4G17500.1; AT4G17500.1; AT4G17500.
KEGGiath:AT4G17500.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z97343 Genomic DNA. Translation: CAB45963.1. Frameshift.
AL161546 Genomic DNA. Translation: CAB78753.1. Frameshift.
CP002687 Genomic DNA. Translation: AEE83903.1.
AY058174 mRNA. Translation: AAL25588.1.
AY062533 mRNA. Translation: AAL32611.1.
BT002578 mRNA. Translation: AAO00938.1.
AB008103 mRNA. Translation: BAA32418.1. Different initiation.
PIRiA85196.
T51988.
RefSeqiNP_567530.4. NM_117855.6.
UniGeneiAt.23185.
At.68141.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GCCNMR-A146-208[»]
2GCCNMR-A143-212[»]
3GCCNMR-A143-212[»]
ProteinModelPortaliO80337.
SMRiO80337.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi12757. 1 interactor.
MINTiMINT-8070094.
STRINGi3702.AT4G17500.1.

Proteomic databases

PaxDbiO80337.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G17500.1; AT4G17500.1; AT4G17500.
GeneIDi827464.
GrameneiAT4G17500.1; AT4G17500.1; AT4G17500.
KEGGiath:AT4G17500.

Organism-specific databases

TAIRiAT4G17500.

Phylogenomic databases

eggNOGiENOG410J07P. Eukaryota.
ENOG410YJV7. LUCA.
HOGENOMiHOG000239314.
InParanoidiO80337.
KOiK09286.
OMAiCEVVEVT.
OrthoDBiEOG09360R86.
PhylomeDBiO80337.

Miscellaneous databases

EvolutionaryTraceiO80337.
PROiO80337.

Gene expression databases

GenevisibleiO80337. AT.

Family and domain databases

CDDicd00018. AP2. 1 hit.
Gene3Di3.30.730.10. 1 hit.
InterProiIPR001471. AP2/ERF_dom.
IPR016177. DNA-bd_dom.
[Graphical view]
PfamiPF00847. AP2. 1 hit.
[Graphical view]
PRINTSiPR00367. ETHRSPELEMNT.
SMARTiSM00380. AP2. 1 hit.
[Graphical view]
SUPFAMiSSF54171. SSF54171. 1 hit.
PROSITEiPS51032. AP2_ERF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEF100_ARATH
AccessioniPrimary (citable) accession number: O80337
Secondary accession number(s): Q93Z36, Q9SUK1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 27, 2002
Last modified: November 30, 2016
This is version 144 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

Two different genes At3g23240 and At4g17500 were assigned the same gene name ERF1.Curated
It is uncertain whether Met-1 or Met-3 is the initiator.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.