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O80337

- EF100_ARATH

UniProt

O80337 - EF100_ARATH

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Protein

Ethylene-responsive transcription factor 1A

Gene

ERF1A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Acts as a transcriptional activator. Binds to the GCC-box pathogenesis-related promoter element. Involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways.3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi147 – 20559AP2/ERFPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. sequence-specific DNA binding transcription factor activity Source: TAIR

GO - Biological processi

  1. defense response Source: UniProtKB-KW
  2. ethylene-activated signaling pathway Source: UniProtKB-KW
  3. response to chitin Source: TAIR
  4. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Ethylene signaling pathway, Plant defense, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ethylene-responsive transcription factor 1A
Short name:
AtERF1A
Alternative name(s):
Ethylene-responsive element-binding factor 1A
Short name:
EREBP-1A
Gene namesi
Name:ERF1A
Synonyms:ERF-1, ERF100
Ordered Locus Names:At4g17500
ORF Names:dl4785w, FCAALL.123
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 4

Organism-specific databases

TAIRiAT4G17500.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 268268Ethylene-responsive transcription factor 1APRO_0000112542Add
BLAST

Proteomic databases

PRIDEiO80337.

Expressioni

Tissue specificityi

Ubiquitously expressed, mostly in flowers and rosettes after ethylene treatment.1 Publication

Inductioni

Induced by Pseudomonas syringae tomato (both virulent and avirulent avrRpt2 strains), independently of PAD4. Also induced by methyl jasmonate (MeJA) independently of JAR1. Ethylene induction is completely dependent on a functional ETHYLENE-INSENSITIVE2 (EIN2), whereas induction by wounding does not need EIN2. Induction by salicylic acid (SA) seems to be independent of PAD4 and NPR1. Transcripts accumulate strongly in cycloheximide-treated plants, a protein synthesis inhibitor. Seems to not be influenced by exogenous abscisic acid (ABA), cold, heat, NaCl or drought stress.2 Publications

Gene expression databases

GenevestigatoriO80337.

Interactioni

Protein-protein interaction databases

MINTiMINT-8070094.

Structurei

Secondary structure

1
268
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi151 – 1544Combined sources
Turni155 – 1573Combined sources
Beta strandi158 – 1658Combined sources
Turni166 – 1694Combined sources
Beta strandi170 – 18011Combined sources
Helixi181 – 19616Combined sources
Beta strandi203 – 2064Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GCCNMR-A146-208[»]
2GCCNMR-A143-212[»]
3GCCNMR-A143-212[»]
ProteinModelPortaliO80337.
SMRiO80337. Positions 146-208.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO80337.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi221 – 23717Nuclear localization signalSequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi98 – 1025Poly-Ser

Domaini

The AP2/ERF domain binds specifically to the 5'-GCCGCC-3' motif. The affinity of this binding is higher if the seventh amino-acid of this domain is basic (By similarity).By similarity

Sequence similaritiesi

Contains 1 AP2/ERF DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG246525.
HOGENOMiHOG000239314.
InParanoidiO80337.
KOiK09286.
OMAiHNDSCRR.
PhylomeDBiO80337.

Family and domain databases

Gene3Di3.30.730.10. 1 hit.
InterProiIPR001471. AP2/ERF_dom.
IPR016177. DNA-bd_dom.
[Graphical view]
PfamiPF00847. AP2. 1 hit.
[Graphical view]
PRINTSiPR00367. ETHRSPELEMNT.
SMARTiSM00380. AP2. 1 hit.
[Graphical view]
SUPFAMiSSF54171. SSF54171. 1 hit.
PROSITEiPS51032. AP2_ERF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O80337-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSMTADSQSD YAFLESIRRH LLGESEPILS ESTASSVTQS CVTGQSIKPV
60 70 80 90 100
YGRNPSFSKL YPCFTESWGD LPLKENDSED MLVYGILNDA FHGGWEPSSS
110 120 130 140 150
SSDEDRSSFP SVKIETPESF AAVDSVPVKK EKTSPVSAAV TAAKGKHYRG
160 170 180 190 200
VRQRPWGKFA AEIRDPAKNG ARVWLGTFET AEDAALAYDR AAFRMRGSRA
210 220 230 240 250
LLNFPLRVNS GEPDPVRIKS KRSSFSSSNE NGAPKKRRTV AAGGGMDKGL
260
TVKCEVVEVA RGDRLLVL
Length:268
Mass (Da):29,190
Last modified:May 27, 2002 - v2
Checksum:i0FB7839984E97B50
GO

Sequence cautioni

The sequence BAA32418.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAB45963.1 differs from that shown. Reason: Frameshift at position 44. Curated
The sequence CAB78753.1 differs from that shown. Reason: Frameshift at position 44. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z97343 Genomic DNA. Translation: CAB45963.1. Frameshift.
AL161546 Genomic DNA. Translation: CAB78753.1. Frameshift.
CP002687 Genomic DNA. Translation: AEE83903.1.
AY058174 mRNA. Translation: AAL25588.1.
AY062533 mRNA. Translation: AAL32611.1.
BT002578 mRNA. Translation: AAO00938.1.
AB008103 mRNA. Translation: BAA32418.1. Different initiation.
PIRiA85196.
T51988.
RefSeqiNP_567530.4. NM_117855.5.
UniGeneiAt.23185.
At.68141.

Genome annotation databases

EnsemblPlantsiAT4G17500.1; AT4G17500.1; AT4G17500.
GeneIDi827464.
KEGGiath:AT4G17500.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z97343 Genomic DNA. Translation: CAB45963.1 . Frameshift.
AL161546 Genomic DNA. Translation: CAB78753.1 . Frameshift.
CP002687 Genomic DNA. Translation: AEE83903.1 .
AY058174 mRNA. Translation: AAL25588.1 .
AY062533 mRNA. Translation: AAL32611.1 .
BT002578 mRNA. Translation: AAO00938.1 .
AB008103 mRNA. Translation: BAA32418.1 . Different initiation.
PIRi A85196.
T51988.
RefSeqi NP_567530.4. NM_117855.5.
UniGenei At.23185.
At.68141.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1GCC NMR - A 146-208 [» ]
2GCC NMR - A 143-212 [» ]
3GCC NMR - A 143-212 [» ]
ProteinModelPortali O80337.
SMRi O80337. Positions 146-208.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-8070094.

Proteomic databases

PRIDEi O80337.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT4G17500.1 ; AT4G17500.1 ; AT4G17500 .
GeneIDi 827464.
KEGGi ath:AT4G17500.

Organism-specific databases

TAIRi AT4G17500.

Phylogenomic databases

eggNOGi NOG246525.
HOGENOMi HOG000239314.
InParanoidi O80337.
KOi K09286.
OMAi HNDSCRR.
PhylomeDBi O80337.

Miscellaneous databases

EvolutionaryTracei O80337.

Gene expression databases

Genevestigatori O80337.

Family and domain databases

Gene3Di 3.30.730.10. 1 hit.
InterProi IPR001471. AP2/ERF_dom.
IPR016177. DNA-bd_dom.
[Graphical view ]
Pfami PF00847. AP2. 1 hit.
[Graphical view ]
PRINTSi PR00367. ETHRSPELEMNT.
SMARTi SM00380. AP2. 1 hit.
[Graphical view ]
SUPFAMi SSF54171. SSF54171. 1 hit.
PROSITEi PS51032. AP2_ERF. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana."
    Bevan M., Bancroft I., Bent E., Love K., Goodman H.M., Dean C., Bergkamp R., Dirkse W., van Staveren M., Stiekema W., Drost L., Ridley P., Hudson S.-A., Patel K., Murphy G., Piffanelli P., Wedler H., Wedler E.
    , Wambutt R., Weitzenegger T., Pohl T., Terryn N., Gielen J., Villarroel R., De Clercq R., van Montagu M., Lecharny A., Aubourg S., Gy I., Kreis M., Lao N., Kavanagh T., Hempel S., Kotter P., Entian K.-D., Rieger M., Schaefer M., Funk B., Mueller-Auer S., Silvey M., James R., Monfort A., Pons A., Puigdomenech P., Douka A., Voukelatou E., Milioni D., Hatzopoulos P., Piravandi E., Obermaier B., Hilbert H., Duesterhoeft A., Moores T., Jones J.D.G., Eneva T., Palme K., Benes V., Rechmann S., Ansorge W., Cooke R., Berger C., Delseny M., Voet M., Volckaert G., Mewes H.-W., Klosterman S., Schueller C., Chalwatzis N.
    Nature 391:485-488(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Arabidopsis ethylene responsive element binding factors act as transcriptional activators or repressors of GCC box mediated gene expression."
    Fujimoto S.Y., Ohta M., Usui A., Shinshi H., Ohme-Takagi M.
    Plant Cell 12:393-404(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-268, FUNCTION, INDUCTION.
    Strain: cv. C24.
  6. "Unique mode of GCC box recognition by the DNA-binding domain of ethylene-responsive element-binding factor (ERF domain) in plant."
    Hao D., Ohme-Takagi M., Sarai A.
    J. Biol. Chem. 273:26857-26861(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Identification of Arabidopsis ethylene-responsive element binding factors with distinct induction kinetics after pathogen infection."
    Onate-Sanchez L., Singh K.B.
    Plant Physiol. 128:1313-1322(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, INDUCTION.
  8. "A novel mode of DNA recognition by a beta-sheet revealed by the solution structure of the GCC-box binding domain in complex with DNA."
    Allen M.D., Yamasaki K., Ohme-Takagi M., Tateno M., Suzuki M.
    EMBO J. 17:5484-5496(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 146-208.
  9. "Genome-wide analysis of the ERF gene family in Arabidopsis and rice."
    Nakano T., Suzuki K., Fujimura T., Shinshi H.
    Plant Physiol. 140:411-432(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiEF100_ARATH
AccessioniPrimary (citable) accession number: O80337
Secondary accession number(s): Q93Z36, Q9SUK1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 27, 2002
Last modified: November 26, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

Two different genes At3g23240 and At4g17500 were assigned the same gene name ERF1.Curated
It is uncertain whether Met-1 or Met-3 is the initiator.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3