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O80337 (EF100_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 126. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Ethylene-responsive transcription factor 1A

Short name=AtERF1A
Alternative name(s):
Ethylene-responsive element-binding factor 1A
Short name=EREBP-1A
Gene names
Name:ERF1A
Synonyms:ERF-1, ERF100
Ordered Locus Names:At4g17500
ORF Names:dl4785w, FCAALL.123
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length268 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a transcriptional activator. Binds to the GCC-box pathogenesis-related promoter element. Involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways. Ref.5 Ref.6 Ref.7

Subcellular location

Nucleus Probable.

Tissue specificity

Ubiquitously expressed, mostly in flowers and rosettes after ethylene treatment. Ref.7

Induction

Induced by Pseudomonas syringae tomato (both virulent and avirulent avrRpt2 strains), independently of PAD4. Also induced by methyl jasmonate (MeJA) independently of JAR1. Ethylene induction is completely dependent on a functional ETHYLENE-INSENSITIVE2 (EIN2), whereas induction by wounding does not need EIN2. Induction by salicylic acid (SA) seems to be independent of PAD4 and NPR1. Transcripts accumulate strongly in cycloheximide-treated plants, a protein synthesis inhibitor. Seems to not be influenced by exogenous abscisic acid (ABA), cold, heat, NaCl or drought stress. Ref.5 Ref.7

Domain

The AP2/ERF domain binds specifically to the 5'-GCCGCC-3' motif. The affinity of this binding is higher if the seventh amino-acid of this domain is basic By similarity.

Sequence similarities

Belongs to the AP2/ERF transcription factor family. ERF subfamily.

Contains 1 AP2/ERF DNA-binding domain.

Caution

Two different genes At3g23240 and At4g17500 were assigned the same gene name ERF1.

It is uncertain whether Met-1 or Met-3 is the initiator.

Sequence caution

The sequence BAA32418.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAB45963.1 differs from that shown. Reason: Frameshift at position 44.

The sequence CAB78753.1 differs from that shown. Reason: Frameshift at position 44.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 268268Ethylene-responsive transcription factor 1A
PRO_0000112542

Regions

DNA binding147 – 20559AP2/ERF
Motif221 – 23717Nuclear localization signal Potential
Compositional bias98 – 1025Poly-Ser

Secondary structure

.......... 268
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O80337 [UniParc].

Last modified May 27, 2002. Version 2.
Checksum: 0FB7839984E97B50

FASTA26829,190
        10         20         30         40         50         60 
MSMTADSQSD YAFLESIRRH LLGESEPILS ESTASSVTQS CVTGQSIKPV YGRNPSFSKL 

        70         80         90        100        110        120 
YPCFTESWGD LPLKENDSED MLVYGILNDA FHGGWEPSSS SSDEDRSSFP SVKIETPESF 

       130        140        150        160        170        180 
AAVDSVPVKK EKTSPVSAAV TAAKGKHYRG VRQRPWGKFA AEIRDPAKNG ARVWLGTFET 

       190        200        210        220        230        240 
AEDAALAYDR AAFRMRGSRA LLNFPLRVNS GEPDPVRIKS KRSSFSSSNE NGAPKKRRTV 

       250        260 
AAGGGMDKGL TVKCEVVEVA RGDRLLVL 

« Hide

References

« Hide 'large scale' references
[1]"Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana."
Bevan M., Bancroft I., Bent E., Love K., Goodman H.M., Dean C., Bergkamp R., Dirkse W., van Staveren M., Stiekema W., Drost L., Ridley P., Hudson S.-A., Patel K., Murphy G., Piffanelli P., Wedler H., Wedler E. expand/collapse author list , Wambutt R., Weitzenegger T., Pohl T., Terryn N., Gielen J., Villarroel R., De Clercq R., van Montagu M., Lecharny A., Aubourg S., Gy I., Kreis M., Lao N., Kavanagh T., Hempel S., Kotter P., Entian K.-D., Rieger M., Schaefer M., Funk B., Mueller-Auer S., Silvey M., James R., Monfort A., Pons A., Puigdomenech P., Douka A., Voukelatou E., Milioni D., Hatzopoulos P., Piravandi E., Obermaier B., Hilbert H., Duesterhoeft A., Moores T., Jones J.D.G., Eneva T., Palme K., Benes V., Rechmann S., Ansorge W., Cooke R., Berger C., Delseny M., Voet M., Volckaert G., Mewes H.-W., Klosterman S., Schueller C., Chalwatzis N.
Nature 391:485-488(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. expand/collapse author list , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Arabidopsis ethylene responsive element binding factors act as transcriptional activators or repressors of GCC box mediated gene expression."
Fujimoto S.Y., Ohta M., Usui A., Shinshi H., Ohme-Takagi M.
Plant Cell 12:393-404(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-268, FUNCTION, INDUCTION.
Strain: cv. C24.
[6]"Unique mode of GCC box recognition by the DNA-binding domain of ethylene-responsive element-binding factor (ERF domain) in plant."
Hao D., Ohme-Takagi M., Sarai A.
J. Biol. Chem. 273:26857-26861(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Identification of Arabidopsis ethylene-responsive element binding factors with distinct induction kinetics after pathogen infection."
Onate-Sanchez L., Singh K.B.
Plant Physiol. 128:1313-1322(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, INDUCTION.
[8]"A novel mode of DNA recognition by a beta-sheet revealed by the solution structure of the GCC-box binding domain in complex with DNA."
Allen M.D., Yamasaki K., Ohme-Takagi M., Tateno M., Suzuki M.
EMBO J. 17:5484-5496(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 146-208.
[9]"Genome-wide analysis of the ERF gene family in Arabidopsis and rice."
Nakano T., Suzuki K., Fujimura T., Shinshi H.
Plant Physiol. 140:411-432(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z97343 Genomic DNA. Translation: CAB45963.1. Frameshift.
AL161546 Genomic DNA. Translation: CAB78753.1. Frameshift.
CP002687 Genomic DNA. Translation: AEE83903.1.
AY058174 mRNA. Translation: AAL25588.1.
AY062533 mRNA. Translation: AAL32611.1.
BT002578 mRNA. Translation: AAO00938.1.
AB008103 mRNA. Translation: BAA32418.1. Different initiation.
PIRA85196.
T51988.
RefSeqNP_567530.4. NM_117855.5.
UniGeneAt.23185.
At.68141.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1GCCNMR-A146-208[»]
2GCCNMR-A143-212[»]
3GCCNMR-A143-212[»]
ProteinModelPortalO80337.
SMRO80337. Positions 146-208.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-8070094.

Proteomic databases

PRIDEO80337.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT4G17500.1; AT4G17500.1; AT4G17500.
GeneID827464.
KEGGath:AT4G17500.

Organism-specific databases

TAIRAT4G17500.

Phylogenomic databases

eggNOGNOG246525.
HOGENOMHOG000239314.
InParanoidO80337.
KOK09286.
OMAHNDSCRR.
PhylomeDBO80337.

Gene expression databases

GenevestigatorO80337.

Family and domain databases

Gene3D3.30.730.10. 1 hit.
InterProIPR001471. AP2/ERF_dom.
IPR016177. DNA-bd_dom.
[Graphical view]
PfamPF00847. AP2. 1 hit.
[Graphical view]
PRINTSPR00367. ETHRSPELEMNT.
SMARTSM00380. AP2. 1 hit.
[Graphical view]
SUPFAMSSF54171. SSF54171. 1 hit.
PROSITEPS51032. AP2_ERF. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceO80337.

Entry information

Entry nameEF100_ARATH
AccessionPrimary (citable) accession number: O80337
Secondary accession number(s): Q93Z36, Q9SUK1
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 27, 2002
Last modified: May 14, 2014
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names