O80323 (RNS3_PYRPY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Ribonuclease S-3 EC=3.1.27.1 Alternative name(s): S3-RNase
Organism	Pyrus pyrifolia (Chinese pear) (Pyrus serotina)
Taxonomic identifier	3767 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Rosales › Rosaceae › Amygdaloideae › Maleae › Pyrus

Protein attributes

Sequence length	222 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Self-incompatibility (SI) is the inherited ability of a flowering plant to prevent self-fertilization by discriminating between self and non-self pollen during pollination. In many species, self-incompatibility is controlled by the single, multiallelic locus S.
Catalytic activity	Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides with 2',3'-cyclic phosphate intermediates.
Post-translational modification	N-linked core structure at Asn-138 contains xylose.
Sequence similarities	Belongs to the RNase T2 family.

Ontologies

Keywords
Domain	Signal
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro ribonuclease T2 activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 22

22

Ref.2

Chain

23 – 222

200

Ribonuclease S-3

PRO_0000030979

Sites

Active site

55

1

Active site

106

1

Active site

109

1

Active site

110

1

Amino acid modifications

Glycosylation

40

1

N-linked (GlcNAc...) Ref.3

Glycosylation

138

1

N-linked (GlcNAc...) Ref.3

Disulfide bond

37 ↔ 44

Disulfide bond

70 ↔ 113

Disulfide bond

177 ↔ 215

Disulfide bond

192 ↔ 203

Secondary structure

1

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222

Helix Strand Turn

Details...

Beta strand

25 – 32

8

Helix

33 – 36

4

Beta strand

39 – 42

4

Beta strand

50 – 60

11

Beta strand

62 – 65

4

Helix

80 – 89

10

Helix

96 – 98

3

Helix

99 – 109

11

Helix

111 – 113

3

Turn

115 – 117

3

Helix

121 – 132

12

Turn

133 – 135

3

Helix

139 – 145

7

Beta strand

153 – 155

3

Helix

157 – 165

9

Turn

166 – 170

5

Beta strand

174 – 180

7

Beta strand

183 – 193

11

Beta strand

197 – 201

5

Beta strand

220 – 222

3

Sequences

	Sequence		Length	Mass (Da)
	O80323 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: C3B3A6FEB53D91C1	FASTA	222	25,747
	10 20 30 40 50 60 MVHVVMMVFL LIVLILCSST VGYDYFQFTQ QYQLAVCNSN RTLCKDPPDK LFTVHGLWPS 70 80 90 100 110 120 NMVGPDPSKC PIKNIRKREK LLEHQLEIIW PNVFDRTKNN LFWDKEWMKH GSCGYPTIDN 130 140 150 160 170 180 ENHYFETVIK MYISKKQNVS RILSKAKIEP DGKKRALLDI ENAIRNGADN KKPKLKCQKK 190 200 210 220 GTTTELVEIT LCSDKSGEHF IDCPHPFEPI SPHYCPTNNI KY

References

[1]	"Primary structural features of rosaceous S-RNases associated with gametophytic self-incompatibility." Ishimizu T., Shinkawa T., Sakiyama F., Norioka S. Plant Mol. Biol. 37:931-941(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: cv. Hosui. Tissue: Style.
[2]	"Identification and characterization of stylar glycoproteins associated with self-incompatibility genes of Japanese pear, Pyrus serotina Rehd." Sassa H., Hirano H., Ikehashi H. Mol. Gen. Genet. 241:17-25(1993) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 23-36.
[3]	"Presence of asparagine-linked N-acetylglucosamine and chitobiose in Pyrus pyrifolia S-RNases associated with gametophytic self-incompatibility." Ishimizu T., Mitsukami Y., Shinkawa T., Natsuka S., Hase S., Miyagi M., Sakiyama F., Norioka S. Eur. J. Biochem. 263:624-634(1999) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION AT ASN-40 AND ASN-138, STRUCTURE OF CARBOHYDRATES. Strain: cv. Hosui. Tissue: Style.
[4]	"Crystal structure at 1.5-A resolution of Pyrus pyrifolia pistil ribonuclease responsible for gametophytic self-incompatibility." Matsuura T., Sakai H., Unno M., Ida K., Sato M., Sakiyama F., Norioka S. J. Biol. Chem. 276:45261-45269(2001) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB002140 mRNA. Translation: BAA32413.1.

PIR

S39930.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1IQQ	X-ray	1.50	A	23-222	[»]

ProteinModelPortal

O80323.

SMR

O80323. Positions 23-222.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

3.90.730.10. 1 hit.

InterPro

IPR001568. RNase_T2-like.
IPR018188. RNase_T2_AS.
[Graphical view]

PANTHER

PTHR11240. PTHR11240. 1 hit.

Pfam

PF00445. Ribonuclease_T2. 1 hit.
[Graphical view]

SUPFAM

SSF55895. RNase_T2. 1 hit.

PROSITE

PS00530. RNASE_T2_1. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

O80323.

Entry information

Entry name

RNS3_PYRPY

Accession

Primary (citable) accession number: O80323

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1998
Last modified:	April 3, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program