Reviewed, UniProtKB/Swiss-Prot O78518 (ILVB_GUITH)

Names and origin

Protein names

Recommended name:
    Acetolactate synthase large subunit
      Short name=AHAS
    EC=2.2.1.6
Alternative name(s):
    Acetohydroxy-acid synthase large subunit
      Short name=ALS

Gene names

Name:

ilvB

Encoded on

Plastid; Chloroplast

Organism

Guillardia theta (Cryptomonas phi)

Taxonomic identifier

55529 [NCBI]

Taxonomic lineage

Eukaryota › Cryptophyta › Pyrenomonadales › Geminigeraceae › Guillardia

Protein attributes

Sequence length	575 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 pyruvate = 2-acetolactate + CO2.
Cofactor	Binds 1 magnesium ion per subunit By similarity. Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4. Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.
Subunit structure	Dimer of large and small chains By similarity.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the TPP enzyme family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Cellular component	Chloroplast Plastid
Ligand	FAD Flavoprotein Magnesium Metal-binding Thiamine pyrophosphate
Molecular function	Transferase
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro acetolactate synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW thiamin pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 575	575	Acetolactate synthase large subunit		PRO_0000090810
Regions
	Nucleotide binding	270 – 291	22	FAD By similarity
	Nucleotide binding	313 – 332	20	FAD By similarity
	Region	404 – 483	80	Thiamine pyrophosphate binding
Sites
	Metal binding	454	1	Magnesium By similarity
	Metal binding	481	1	Magnesium By similarity
	Binding site	60	1	Thiamine pyrophosphate By similarity
	Binding site	162	1	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	O78518-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: F9447F9A178D3EC4	FASTA	575	63,360
	10 20 30 40 50 60 MPNILKNRDT TGAFALIDSL VRHGVKHIFG YPGGAILPIY DELYAWEKEG FIEHILVRHE 70 80 90 100 110 120 QGAAHASDGY ARSTGNVGVC FATSGPGATN LVTGIATAHM DSVPMVIITG QVGRSFIGTD 130 140 150 160 170 180 AFQEVDIFGI TLPIVKHSYV VRETKEMGKI VAESFFIAKY GRPGPVLIDI PKDVGLEKFD 190 200 210 220 230 240 YQIVNPNNIN LAGCPVLKNY DQNRISQAAN LIKQSSQPLL YIGGGAVTSN SHNEINELIN 250 260 270 280 290 300 LVKIPVATTL MGKGIIDESH PLSLGMLGMH GTVYANYAVS ECDLLIALGA RFDDRVTGKL 310 320 330 340 350 360 DEFACHAQVI HVDIDPAEIG KNRTPQIGIV GEIKDFVRDL IECLKNDINF DSEQSQAWRS 370 380 390 400 410 420 RIIRWRKEYP LLVPKNINNL SPQEVIHEIS TEATNAYFTT DVGQHQMWAA QFIKTSQKRW 430 440 450 460 470 480 ITSAGLGTMG YGLPAAIGVQ IAHPNEQVIC ISGDASFQMN IQELGTVSQY GLPIKIFIIN 490 500 510 520 530 540 NKWQGMVRQW QQAFYGERYS HSNMEKGAPN FTKVAEAFGL RSLKIKSRND LKLRIKEALD 550 560 570 YDGPILVDIQ VIADENCYPM VAPGKSNAQM MGINS

References

[1]	"The plastid genome of the cryptophyte alga, Guillardia theta: complete sequence and conserved synteny groups confirm its common ancestry with red algae." Douglas S.E., Penny S.L. J. Mol. Evol. 48:236-244(1999) [PubMed: 9929392] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF041468 Genomic DNA. Translation: AAC35740.1.
RefSeq	NP_050806.1.
3D structure databases
HSSP	HSSP built from PDB template 1JSC based on UniProtKB P07342.
ModBase	Search...
Genome annotation databases
GeneID	857117.
Phylogenomic databases
HOGENOM	O78518.
OMA	O78518. HSWRYDH.
Enzyme and pathway databases
BRENDA	2.2.1.6. 279957.
Family and domain databases
InterPro	IPR012846. Acetolactate_synth_lsu. IPR000399. TPP_bd_CS. IPR012001. TPP_bd_enzyme_N. IPR011766. TPP_enzyme_bd_C. IPR012000. TPP_enzyme_M. [Graphical view]
Pfam	PF02775. TPP_enzyme_C. 1 hit. PF00205. TPP_enzyme_M. 1 hit. PF02776. TPP_enzyme_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00118. acolac_lg. 1 hit.
PROSITE	PS00187. TPP_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVB_GUITH

Accession

Primary (citable) accession number: O78518

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1998
Last modified:	June 16, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)