ID   DNAB_GUITH              Reviewed;         599 AA.
AC   O78411;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   13-SEP-2023, entry version 119.
DE   RecName: Full=Probable replicative DNA helicase;
DE            EC=3.6.4.12;
DE   Contains:
DE     RecName: Full=Gth dnaB intein;
GN   Name=dnaB;
OS   Guillardia theta (Cryptophyte) (Cryptomonas phi).
OG   Plastid; Chloroplast.
OC   Eukaryota; Cryptophyceae; Pyrenomonadales; Geminigeraceae; Guillardia.
OX   NCBI_TaxID=55529;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=9929392; DOI=10.1007/pl00006462;
RA   Douglas S.E., Penny S.L.;
RT   "The plastid genome of the cryptophyte alga, Guillardia theta: complete
RT   sequence and conserved synteny groups confirm its common ancestry with red
RT   algae.";
RL   J. Mol. Evol. 48:236-244(1999).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- PTM: This protein undergoes a protein self splicing that involves a
CC       post-translational excision of the intervening region (intein) followed
CC       by peptide ligation. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the helicase family. DnaB subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AF041468; AAC35596.1; -; Genomic_DNA.
DR   RefSeq; NP_050662.1; NC_000926.1.
DR   AlphaFoldDB; O78411; -.
DR   SMR; O78411; -.
DR   GeneID; 856947; -.
DR   HOGENOM; CLU_005373_0_0_1; -.
DR   OMA; IEFHARI; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR   GO; GO:0006269; P:DNA replication, synthesis of RNA primer; IEA:UniProtKB-KW.
DR   CDD; cd00984; DnaB_C; 1.
DR   Gene3D; 2.170.16.10; Hedgehog/Intein (Hint) domain; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR   InterPro; IPR036185; DNA_heli_DnaB-like_N_sf.
DR   InterPro; IPR007692; DNA_helicase_DnaB.
DR   InterPro; IPR007694; DNA_helicase_DnaB-like_C.
DR   InterPro; IPR007693; DNA_helicase_DnaB-like_N.
DR   InterPro; IPR016136; DNA_helicase_N/primase_C.
DR   InterPro; IPR003586; Hint_dom_C.
DR   InterPro; IPR036844; Hint_dom_sf.
DR   InterPro; IPR030934; Intein_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   NCBIfam; TIGR00665; DnaB; 1.
DR   PANTHER; PTHR30153:SF2; REPLICATIVE DNA HELICASE; 1.
DR   PANTHER; PTHR30153; REPLICATIVE DNA HELICASE DNAB; 1.
DR   Pfam; PF00772; DnaB; 1.
DR   Pfam; PF03796; DnaB_C; 1.
DR   SMART; SM00305; HintC; 1.
DR   SUPFAM; SSF51294; Hedgehog/intein (Hint) domain; 1.
DR   SUPFAM; SSF48024; N-terminal domain of DnaB helicase; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   PROSITE; PS50818; INTEIN_C_TER; 1.
DR   PROSITE; PS51199; SF4_HELICASE; 2.
PE   3: Inferred from homology;
KW   ATP-binding; Autocatalytic cleavage; Chloroplast; DNA replication;
KW   DNA-binding; Helicase; Hydrolase; Nucleotide-binding; Plastid; Primosome;
KW   Protein splicing; Repeat.
FT   CHAIN           1..376
FT                   /note="Probable replicative DNA helicase, 1st part"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000013291"
FT   CHAIN           377..536
FT                   /note="Gth dnaB intein"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000013292"
FT   CHAIN           537..599
FT                   /note="Probable replicative DNA helicase, 2nd part"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000013293"
FT   DOMAIN          171..439
FT                   /note="SF4 helicase; first part"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00596"
FT   DOMAIN          333..599
FT                   /note="SF4 helicase; second part"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00596"
FT   BINDING         202..209
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00596"
SQ   SEQUENCE   599 AA;  69445 MW;  3018EB679E0FDCD5 CRC64;
     MKVTKFYNLK AEKHLIIQIL VEKDFFLQIY TKIDTQVFYF QSHQILYRSI NILHTKKVLI
     NLPNLLSLLH SELVSETVIN YLITLFNDRD NFRILNINDS LKVLLDNFIR RELQNSCAKI
     VSLTFNFNLS VETLLQKSNL LISNINTYNK KVSLKSISQL LLETILEIDK KTNRSTHVLT
     GFFDLDHILV GLQKSDLIII AGRPSMGKTA FMLSLVRNVA DIQSFPIVIF SLEMSSKQLI
     YRLISNETNI ATSRLREGNI SISEWEILNR AMTILSNLNI YLDDENNLDV LDIQSKLASL
     QQIYGDIGLI VIDYLQLLQY KEKSSQRHIE LSYITRYLKL IAKDFNLPLV VLSQLSRNVE
     FRLNKRPILS DLKESGCLSY ATNQPYFLKS DNVNFSKLTS LKVSNHYILS ATLELLIPFQ
     YNRIYPIVSL IKRELQTGYK VVYELDFYIS VIVSTVEHYV LTLNGWKRIL ELTVDDLVAT
     LDIQYLIYNN TEVDLFSSNV IFSSVINLIC MNRINVYDFW IPKTNNFFVN ALLVHNSIEQ
     DADVVLFLYR DSYYQHNLKQ SNIDMCEVIV AKHRHGTIGM VNLIFNPNTV SFMNLIKES
//