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O78411

- DNAB_GUITH

UniProt

O78411 - DNAB_GUITH

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Protein
Probable replicative DNA helicase
Gene
dnaB
Organism
Guillardia theta (Cryptomonas phi)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi202 – 2098ATP Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA helicase activity Source: InterPro

GO - Biological processi

  1. DNA replication, synthesis of RNA primer Source: UniProtKB-KW
  2. intein-mediated protein splicing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable replicative DNA helicase (EC:3.6.4.12)
Cleaved into the following chain:
Gene namesi
Name:dnaB
Encoded oniPlastid; Chloroplast
OrganismiGuillardia theta (Cryptomonas phi)
Taxonomic identifieri55529 [NCBI]
Taxonomic lineageiEukaryotaCryptophytaPyrenomonadalesGeminigeraceaeGuillardia

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-SubCell
  2. primosome complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid, Primosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 376376Probable replicative DNA helicase, 1st part Reviewed prediction
PRO_0000013291Add
BLAST
Chaini377 – 536160Gth dnaB intein Reviewed prediction
PRO_0000013292Add
BLAST
Chaini537 – 59963Probable replicative DNA helicase, 2nd part Reviewed prediction
PRO_0000013293Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Reviewed prediction.

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Structurei

3D structure databases

ProteinModelPortaliO78411.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini171 – 439269SF4 helicase; first part
Add
BLAST
Domaini333 – 599267SF4 helicase; second part
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000113195.
OMAiNISISEW.

Family and domain databases

Gene3Di1.10.860.10. 1 hit.
2.170.16.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR007692. DNA_helicase_DnaB.
IPR007694. DNA_helicase_DnaB-like_C.
IPR007693. DNA_helicase_DnaB-like_N.
IPR016136. DNA_helicase_N/primase_C.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00772. DnaB. 1 hit.
PF03796. DnaB_C. 1 hit.
[Graphical view]
SMARTiSM00305. HintC. 1 hit.
[Graphical view]
SUPFAMiSSF48024. SSF48024. 1 hit.
SSF51294. SSF51294. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00665. DnaB. 1 hit.
PROSITEiPS50818. INTEIN_C_TER. 1 hit.
PS51199. SF4_HELICASE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O78411-1 [UniParc]FASTAAdd to Basket

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MKVTKFYNLK AEKHLIIQIL VEKDFFLQIY TKIDTQVFYF QSHQILYRSI    50
NILHTKKVLI NLPNLLSLLH SELVSETVIN YLITLFNDRD NFRILNINDS 100
LKVLLDNFIR RELQNSCAKI VSLTFNFNLS VETLLQKSNL LISNINTYNK 150
KVSLKSISQL LLETILEIDK KTNRSTHVLT GFFDLDHILV GLQKSDLIII 200
AGRPSMGKTA FMLSLVRNVA DIQSFPIVIF SLEMSSKQLI YRLISNETNI 250
ATSRLREGNI SISEWEILNR AMTILSNLNI YLDDENNLDV LDIQSKLASL 300
QQIYGDIGLI VIDYLQLLQY KEKSSQRHIE LSYITRYLKL IAKDFNLPLV 350
VLSQLSRNVE FRLNKRPILS DLKESGCLSY ATNQPYFLKS DNVNFSKLTS 400
LKVSNHYILS ATLELLIPFQ YNRIYPIVSL IKRELQTGYK VVYELDFYIS 450
VIVSTVEHYV LTLNGWKRIL ELTVDDLVAT LDIQYLIYNN TEVDLFSSNV 500
IFSSVINLIC MNRINVYDFW IPKTNNFFVN ALLVHNSIEQ DADVVLFLYR 550
DSYYQHNLKQ SNIDMCEVIV AKHRHGTIGM VNLIFNPNTV SFMNLIKES 599
Length:599
Mass (Da):69,445
Last modified:November 1, 1998 - v1
Checksum:i3018EB679E0FDCD5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF041468 Genomic DNA. Translation: AAC35596.1.
RefSeqiNP_050662.1. NC_000926.1.

Genome annotation databases

GeneIDi856947.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF041468 Genomic DNA. Translation: AAC35596.1 .
RefSeqi NP_050662.1. NC_000926.1.

3D structure databases

ProteinModelPortali O78411.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 856947.

Phylogenomic databases

HOGENOMi HOG000113195.
OMAi NISISEW.

Family and domain databases

Gene3Di 1.10.860.10. 1 hit.
2.170.16.10. 1 hit.
3.40.50.300. 2 hits.
InterProi IPR007692. DNA_helicase_DnaB.
IPR007694. DNA_helicase_DnaB-like_C.
IPR007693. DNA_helicase_DnaB-like_N.
IPR016136. DNA_helicase_N/primase_C.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00772. DnaB. 1 hit.
PF03796. DnaB_C. 1 hit.
[Graphical view ]
SMARTi SM00305. HintC. 1 hit.
[Graphical view ]
SUPFAMi SSF48024. SSF48024. 1 hit.
SSF51294. SSF51294. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00665. DnaB. 1 hit.
PROSITEi PS50818. INTEIN_C_TER. 1 hit.
PS51199. SF4_HELICASE. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The plastid genome of the cryptophyte alga, Guillardia theta: complete sequence and conserved synteny groups confirm its common ancestry with red algae."
    Douglas S.E., Penny S.L.
    J. Mol. Evol. 48:236-244(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiDNAB_GUITH
AccessioniPrimary (citable) accession number: O78411
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1998
Last modified: June 11, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3