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O78411

- DNAB_GUITH

UniProt

O78411 - DNAB_GUITH

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Protein

Probable replicative DNA helicase

Gene

dnaB

Organism
Guillardia theta (Cryptomonas phi)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi202 – 2098ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA helicase activity Source: InterPro

GO - Biological processi

  1. DNA replication, synthesis of RNA primer Source: UniProtKB-KW
  2. intein-mediated protein splicing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable replicative DNA helicase (EC:3.6.4.12)
Cleaved into the following chain:
Gene namesi
Name:dnaB
Encoded oniPlastid; Chloroplast
OrganismiGuillardia theta (Cryptomonas phi)
Taxonomic identifieri55529 [NCBI]
Taxonomic lineageiEukaryotaCryptophytaPyrenomonadalesGeminigeraceaeGuillardia

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-KW
  2. primosome complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid, Primosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 376376Probable replicative DNA helicase, 1st partSequence AnalysisPRO_0000013291Add
BLAST
Chaini377 – 536160Gth dnaB inteinSequence AnalysisPRO_0000013292Add
BLAST
Chaini537 – 59963Probable replicative DNA helicase, 2nd partSequence AnalysisPRO_0000013293Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Structurei

3D structure databases

ProteinModelPortaliO78411.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini171 – 439269SF4 helicase; first partPROSITE-ProRule annotationAdd
BLAST
Domaini333 – 599267SF4 helicase; second partPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the helicase family. DnaB subfamily.Curated
Contains 1 SF4 helicase domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000113195.
OMAiNISISEW.

Family and domain databases

Gene3Di1.10.860.10. 1 hit.
2.170.16.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR007692. DNA_helicase_DnaB.
IPR007694. DNA_helicase_DnaB-like_C.
IPR007693. DNA_helicase_DnaB-like_N.
IPR016136. DNA_helicase_N/primase_C.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00772. DnaB. 1 hit.
PF03796. DnaB_C. 1 hit.
[Graphical view]
SMARTiSM00305. HintC. 1 hit.
[Graphical view]
SUPFAMiSSF48024. SSF48024. 1 hit.
SSF51294. SSF51294. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00665. DnaB. 1 hit.
PROSITEiPS50818. INTEIN_C_TER. 1 hit.
PS51199. SF4_HELICASE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O78411-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKVTKFYNLK AEKHLIIQIL VEKDFFLQIY TKIDTQVFYF QSHQILYRSI
60 70 80 90 100
NILHTKKVLI NLPNLLSLLH SELVSETVIN YLITLFNDRD NFRILNINDS
110 120 130 140 150
LKVLLDNFIR RELQNSCAKI VSLTFNFNLS VETLLQKSNL LISNINTYNK
160 170 180 190 200
KVSLKSISQL LLETILEIDK KTNRSTHVLT GFFDLDHILV GLQKSDLIII
210 220 230 240 250
AGRPSMGKTA FMLSLVRNVA DIQSFPIVIF SLEMSSKQLI YRLISNETNI
260 270 280 290 300
ATSRLREGNI SISEWEILNR AMTILSNLNI YLDDENNLDV LDIQSKLASL
310 320 330 340 350
QQIYGDIGLI VIDYLQLLQY KEKSSQRHIE LSYITRYLKL IAKDFNLPLV
360 370 380 390 400
VLSQLSRNVE FRLNKRPILS DLKESGCLSY ATNQPYFLKS DNVNFSKLTS
410 420 430 440 450
LKVSNHYILS ATLELLIPFQ YNRIYPIVSL IKRELQTGYK VVYELDFYIS
460 470 480 490 500
VIVSTVEHYV LTLNGWKRIL ELTVDDLVAT LDIQYLIYNN TEVDLFSSNV
510 520 530 540 550
IFSSVINLIC MNRINVYDFW IPKTNNFFVN ALLVHNSIEQ DADVVLFLYR
560 570 580 590
DSYYQHNLKQ SNIDMCEVIV AKHRHGTIGM VNLIFNPNTV SFMNLIKES
Length:599
Mass (Da):69,445
Last modified:November 1, 1998 - v1
Checksum:i3018EB679E0FDCD5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF041468 Genomic DNA. Translation: AAC35596.1.
RefSeqiNP_050662.1. NC_000926.1.

Genome annotation databases

GeneIDi856947.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF041468 Genomic DNA. Translation: AAC35596.1 .
RefSeqi NP_050662.1. NC_000926.1.

3D structure databases

ProteinModelPortali O78411.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 856947.

Phylogenomic databases

HOGENOMi HOG000113195.
OMAi NISISEW.

Family and domain databases

Gene3Di 1.10.860.10. 1 hit.
2.170.16.10. 1 hit.
3.40.50.300. 2 hits.
InterProi IPR007692. DNA_helicase_DnaB.
IPR007694. DNA_helicase_DnaB-like_C.
IPR007693. DNA_helicase_DnaB-like_N.
IPR016136. DNA_helicase_N/primase_C.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00772. DnaB. 1 hit.
PF03796. DnaB_C. 1 hit.
[Graphical view ]
SMARTi SM00305. HintC. 1 hit.
[Graphical view ]
SUPFAMi SSF48024. SSF48024. 1 hit.
SSF51294. SSF51294. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00665. DnaB. 1 hit.
PROSITEi PS50818. INTEIN_C_TER. 1 hit.
PS51199. SF4_HELICASE. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The plastid genome of the cryptophyte alga, Guillardia theta: complete sequence and conserved synteny groups confirm its common ancestry with red algae."
    Douglas S.E., Penny S.L.
    J. Mol. Evol. 48:236-244(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiDNAB_GUITH
AccessioniPrimary (citable) accession number: O78411
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1998
Last modified: October 29, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3