O78411 (DNAB_GUITH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable replicative DNA helicase
EC=3.6.4.12

Cleaved into the following chain:

1. Gth dnaB intein

Gene names

Name:

dnaB

Encoded on

Plastid; Chloroplast

Organism

Guillardia theta (Cryptomonas phi)

Taxonomic identifier

55529 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Cryptophyta › Pyrenomonadales › Geminigeraceae › Guillardia

Protein attributes

Sequence length	599 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + H2O = ADP + phosphate.
Subcellular location	Plastid › chloroplast.
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential.
Sequence similarities	Belongs to the helicase family. DnaB subfamily. Contains 1 SF4 helicase domain.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Chloroplast Plastid Primosome
Domain	Repeat
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
PTM	Autocatalytic cleavage Protein splicing
Gene Ontology (GO)
Biological_process	DNA duplex unwinding Inferred from electronic annotation. Source: GOC DNA replication, synthesis of RNA primer Inferred from electronic annotation. Source: UniProtKB-KW intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro
Cellular_component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell replisome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW DNA helicase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 376	376	Probable replicative DNA helicase, 1st part Potential		PRO_0000013291
	Chain	377 – 536	160	Gth dnaB intein Potential		PRO_0000013292
	Chain	537 – 599	63	Probable replicative DNA helicase, 2nd part Potential		PRO_0000013293
Regions
	Domain	171 – 439	269	SF4 helicase; first part
	Domain	333 – 599	267	SF4 helicase; second part
	Nucleotide binding	202 – 209	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	O78411 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 3018EB679E0FDCD5	FASTA	599	69,445
	10 20 30 40 50 60 MKVTKFYNLK AEKHLIIQIL VEKDFFLQIY TKIDTQVFYF QSHQILYRSI NILHTKKVLI 70 80 90 100 110 120 NLPNLLSLLH SELVSETVIN YLITLFNDRD NFRILNINDS LKVLLDNFIR RELQNSCAKI 130 140 150 160 170 180 VSLTFNFNLS VETLLQKSNL LISNINTYNK KVSLKSISQL LLETILEIDK KTNRSTHVLT 190 200 210 220 230 240 GFFDLDHILV GLQKSDLIII AGRPSMGKTA FMLSLVRNVA DIQSFPIVIF SLEMSSKQLI 250 260 270 280 290 300 YRLISNETNI ATSRLREGNI SISEWEILNR AMTILSNLNI YLDDENNLDV LDIQSKLASL 310 320 330 340 350 360 QQIYGDIGLI VIDYLQLLQY KEKSSQRHIE LSYITRYLKL IAKDFNLPLV VLSQLSRNVE 370 380 390 400 410 420 FRLNKRPILS DLKESGCLSY ATNQPYFLKS DNVNFSKLTS LKVSNHYILS ATLELLIPFQ 430 440 450 460 470 480 YNRIYPIVSL IKRELQTGYK VVYELDFYIS VIVSTVEHYV LTLNGWKRIL ELTVDDLVAT 490 500 510 520 530 540 LDIQYLIYNN TEVDLFSSNV IFSSVINLIC MNRINVYDFW IPKTNNFFVN ALLVHNSIEQ 550 560 570 580 590 DADVVLFLYR DSYYQHNLKQ SNIDMCEVIV AKHRHGTIGM VNLIFNPNTV SFMNLIKES

References

[1]

"The plastid genome of the cryptophyte alga, Guillardia theta: complete sequence and conserved synteny groups confirm its common ancestry with red algae." Douglas S.E., Penny S.L. J. Mol. Evol. 48:236-244(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF041468 Genomic DNA. Translation: AAC35596.1.
RefSeq	NP_050662.1. NC_000926.1.
3D structure databases
ProteinModelPortal	O78411.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	856947.
Phylogenomic databases
HOGENOM	HOG000113195.
OMA	NISISEW.
ProtClustDB	CLSC858394.
Family and domain databases
Gene3D	1.10.860.10. 1 hit.
InterPro	IPR007692. DNA_helicase_DnaB. IPR007694. DNA_helicase_DnaB-like_C. IPR007693. DNA_helicase_DnaB-like_N. IPR016136. DNA_helicase_N/primase_C. IPR003586. Hint_dom_C. IPR006141. Intein_splice_site. [Graphical view]
Pfam	PF00772. DnaB. 1 hit. PF03796. DnaB_C. 2 hits. [Graphical view]
SMART	SM00305. HintC. 1 hit. [Graphical view]
SUPFAM	SSF48024. DNA_helicase_DnaB-like_N. 1 hit.
TIGRFAMs	TIGR00665. DnaB. 1 hit.
PROSITE	PS50818. INTEIN_C_TER. 1 hit. PS50817. INTEIN_N_TER. False negative. PS51199. SF4_HELICASE. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNAB_GUITH

Accession

Primary (citable) accession number: O78411

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1998
Last modified:	May 1, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)