Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O78411

- DNAB_GUITH

UniProt

O78411 - DNAB_GUITH

Protein

Probable replicative DNA helicase

Gene

dnaB

Organism
Guillardia theta (Cryptomonas phi)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi202 – 2098ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. DNA binding Source: UniProtKB-KW
    3. DNA helicase activity Source: InterPro

    GO - Biological processi

    1. DNA replication, synthesis of RNA primer Source: UniProtKB-KW
    2. intein-mediated protein splicing Source: InterPro

    Keywords - Molecular functioni

    Helicase, Hydrolase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    ATP-binding, DNA-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable replicative DNA helicase (EC:3.6.4.12)
    Cleaved into the following chain:
    Gene namesi
    Name:dnaB
    Encoded oniPlastid; Chloroplast
    OrganismiGuillardia theta (Cryptomonas phi)
    Taxonomic identifieri55529 [NCBI]
    Taxonomic lineageiEukaryotaCryptophytaPyrenomonadalesGeminigeraceaeGuillardia

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: UniProtKB-SubCell
    2. primosome complex Source: UniProtKB-KW

    Keywords - Cellular componenti

    Chloroplast, Plastid, Primosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 376376Probable replicative DNA helicase, 1st partSequence AnalysisPRO_0000013291Add
    BLAST
    Chaini377 – 536160Gth dnaB inteinSequence AnalysisPRO_0000013292Add
    BLAST
    Chaini537 – 59963Probable replicative DNA helicase, 2nd partSequence AnalysisPRO_0000013293Add
    BLAST

    Post-translational modificationi

    This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

    Keywords - PTMi

    Autocatalytic cleavage, Protein splicing

    Structurei

    3D structure databases

    ProteinModelPortaliO78411.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini171 – 439269SF4 helicase; first partPROSITE-ProRule annotationAdd
    BLAST
    Domaini333 – 599267SF4 helicase; second partPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the helicase family. DnaB subfamily.Curated
    Contains 1 SF4 helicase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    HOGENOMiHOG000113195.
    OMAiNISISEW.

    Family and domain databases

    Gene3Di1.10.860.10. 1 hit.
    2.170.16.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProiIPR007692. DNA_helicase_DnaB.
    IPR007694. DNA_helicase_DnaB-like_C.
    IPR007693. DNA_helicase_DnaB-like_N.
    IPR016136. DNA_helicase_N/primase_C.
    IPR028992. Hedgehog/Intein_dom.
    IPR003586. Hint_dom_C.
    IPR006141. Intein_splice_site.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00772. DnaB. 1 hit.
    PF03796. DnaB_C. 1 hit.
    [Graphical view]
    SMARTiSM00305. HintC. 1 hit.
    [Graphical view]
    SUPFAMiSSF48024. SSF48024. 1 hit.
    SSF51294. SSF51294. 1 hit.
    SSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00665. DnaB. 1 hit.
    PROSITEiPS50818. INTEIN_C_TER. 1 hit.
    PS51199. SF4_HELICASE. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O78411-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKVTKFYNLK AEKHLIIQIL VEKDFFLQIY TKIDTQVFYF QSHQILYRSI    50
    NILHTKKVLI NLPNLLSLLH SELVSETVIN YLITLFNDRD NFRILNINDS 100
    LKVLLDNFIR RELQNSCAKI VSLTFNFNLS VETLLQKSNL LISNINTYNK 150
    KVSLKSISQL LLETILEIDK KTNRSTHVLT GFFDLDHILV GLQKSDLIII 200
    AGRPSMGKTA FMLSLVRNVA DIQSFPIVIF SLEMSSKQLI YRLISNETNI 250
    ATSRLREGNI SISEWEILNR AMTILSNLNI YLDDENNLDV LDIQSKLASL 300
    QQIYGDIGLI VIDYLQLLQY KEKSSQRHIE LSYITRYLKL IAKDFNLPLV 350
    VLSQLSRNVE FRLNKRPILS DLKESGCLSY ATNQPYFLKS DNVNFSKLTS 400
    LKVSNHYILS ATLELLIPFQ YNRIYPIVSL IKRELQTGYK VVYELDFYIS 450
    VIVSTVEHYV LTLNGWKRIL ELTVDDLVAT LDIQYLIYNN TEVDLFSSNV 500
    IFSSVINLIC MNRINVYDFW IPKTNNFFVN ALLVHNSIEQ DADVVLFLYR 550
    DSYYQHNLKQ SNIDMCEVIV AKHRHGTIGM VNLIFNPNTV SFMNLIKES 599
    Length:599
    Mass (Da):69,445
    Last modified:November 1, 1998 - v1
    Checksum:i3018EB679E0FDCD5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF041468 Genomic DNA. Translation: AAC35596.1.
    RefSeqiNP_050662.1. NC_000926.1.

    Genome annotation databases

    GeneIDi856947.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF041468 Genomic DNA. Translation: AAC35596.1 .
    RefSeqi NP_050662.1. NC_000926.1.

    3D structure databases

    ProteinModelPortali O78411.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 856947.

    Phylogenomic databases

    HOGENOMi HOG000113195.
    OMAi NISISEW.

    Family and domain databases

    Gene3Di 1.10.860.10. 1 hit.
    2.170.16.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProi IPR007692. DNA_helicase_DnaB.
    IPR007694. DNA_helicase_DnaB-like_C.
    IPR007693. DNA_helicase_DnaB-like_N.
    IPR016136. DNA_helicase_N/primase_C.
    IPR028992. Hedgehog/Intein_dom.
    IPR003586. Hint_dom_C.
    IPR006141. Intein_splice_site.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00772. DnaB. 1 hit.
    PF03796. DnaB_C. 1 hit.
    [Graphical view ]
    SMARTi SM00305. HintC. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48024. SSF48024. 1 hit.
    SSF51294. SSF51294. 1 hit.
    SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00665. DnaB. 1 hit.
    PROSITEi PS50818. INTEIN_C_TER. 1 hit.
    PS51199. SF4_HELICASE. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The plastid genome of the cryptophyte alga, Guillardia theta: complete sequence and conserved synteny groups confirm its common ancestry with red algae."
      Douglas S.E., Penny S.L.
      J. Mol. Evol. 48:236-244(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

    Entry informationi

    Entry nameiDNAB_GUITH
    AccessioniPrimary (citable) accession number: O78411
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Documents

    1. Intein-containing proteins
      List of intein-containing protein entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3