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O78411 (DNAB_GUITH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable replicative DNA helicase

EC=3.6.4.12

Cleaved into the following chain:

  1. Gth dnaB intein
Gene names
Name:dnaB
Encoded onPlastid; Chloroplast
OrganismGuillardia theta (Cryptomonas phi)
Taxonomic identifier55529 [NCBI]
Taxonomic lineageEukaryotaCryptophytaPyrenomonadalesGeminigeraceaeGuillardia

Protein attributes

Sequence length599 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + H2O = ADP + phosphate.

Subcellular location

Plastidchloroplast.

Post-translational modification

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential.

Sequence similarities

Belongs to the helicase family. DnaB subfamily.

Contains 1 SF4 helicase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 376376Probable replicative DNA helicase, 1st part Potential
PRO_0000013291
Chain377 – 536160Gth dnaB intein Potential
PRO_0000013292
Chain537 – 59963Probable replicative DNA helicase, 2nd part Potential
PRO_0000013293

Regions

Domain171 – 439269SF4 helicase; first part
Domain333 – 599267SF4 helicase; second part
Nucleotide binding202 – 2098ATP Potential

Sequences

Sequence LengthMass (Da)Tools
O78411 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 3018EB679E0FDCD5

FASTA59969,445
        10         20         30         40         50         60 
MKVTKFYNLK AEKHLIIQIL VEKDFFLQIY TKIDTQVFYF QSHQILYRSI NILHTKKVLI 

        70         80         90        100        110        120 
NLPNLLSLLH SELVSETVIN YLITLFNDRD NFRILNINDS LKVLLDNFIR RELQNSCAKI 

       130        140        150        160        170        180 
VSLTFNFNLS VETLLQKSNL LISNINTYNK KVSLKSISQL LLETILEIDK KTNRSTHVLT 

       190        200        210        220        230        240 
GFFDLDHILV GLQKSDLIII AGRPSMGKTA FMLSLVRNVA DIQSFPIVIF SLEMSSKQLI 

       250        260        270        280        290        300 
YRLISNETNI ATSRLREGNI SISEWEILNR AMTILSNLNI YLDDENNLDV LDIQSKLASL 

       310        320        330        340        350        360 
QQIYGDIGLI VIDYLQLLQY KEKSSQRHIE LSYITRYLKL IAKDFNLPLV VLSQLSRNVE 

       370        380        390        400        410        420 
FRLNKRPILS DLKESGCLSY ATNQPYFLKS DNVNFSKLTS LKVSNHYILS ATLELLIPFQ 

       430        440        450        460        470        480 
YNRIYPIVSL IKRELQTGYK VVYELDFYIS VIVSTVEHYV LTLNGWKRIL ELTVDDLVAT 

       490        500        510        520        530        540 
LDIQYLIYNN TEVDLFSSNV IFSSVINLIC MNRINVYDFW IPKTNNFFVN ALLVHNSIEQ 

       550        560        570        580        590 
DADVVLFLYR DSYYQHNLKQ SNIDMCEVIV AKHRHGTIGM VNLIFNPNTV SFMNLIKES 

« Hide

References

[1]"The plastid genome of the cryptophyte alga, Guillardia theta: complete sequence and conserved synteny groups confirm its common ancestry with red algae."
Douglas S.E., Penny S.L.
J. Mol. Evol. 48:236-244(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF041468 Genomic DNA. Translation: AAC35596.1.
RefSeqNP_050662.1. NC_000926.1.

3D structure databases

ProteinModelPortalO78411.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID856947.

Phylogenomic databases

HOGENOMHOG000113195.
OMANISISEW.

Family and domain databases

Gene3D1.10.860.10. 1 hit.
2.170.16.10. 1 hit.
3.40.50.300. 2 hits.
InterProIPR007692. DNA_helicase_DnaB.
IPR007694. DNA_helicase_DnaB-like_C.
IPR007693. DNA_helicase_DnaB-like_N.
IPR016136. DNA_helicase_N/primase_C.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF00772. DnaB. 1 hit.
PF03796. DnaB_C. 1 hit.
[Graphical view]
SMARTSM00305. HintC. 1 hit.
[Graphical view]
SUPFAMSSF48024. SSF48024. 1 hit.
SSF51294. SSF51294. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00665. DnaB. 1 hit.
PROSITEPS50818. INTEIN_C_TER. 1 hit.
PS51199. SF4_HELICASE. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNAB_GUITH
AccessionPrimary (citable) accession number: O78411
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1998
Last modified: June 11, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Intein-containing proteins

List of intein-containing protein entries