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Protein

Decapping and exoribonuclease protein

Gene

DXO

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Ribonuclease that specifically degrades pre-mRNAs with a defective 5' end cap and is part of a pre-mRNA capping quality control. Has decapping, pyrophosphohydrolase and 5'-3' exonuclease activities. Has decapping activity toward incomplete 5' end cap mRNAs such as unmethylated 5' end-capped RNA to release GpppN and 5' end monophosphate RNA. The 5' end monophosphate RNA is then degraded by the 5'-3' exoribonuclease activity, enabling this enzyme to decap and degrade incompletely capped mRNAs. Also possesses RNA 5'-pyrophosphohydrolase activity by hydrolyzing the 5' end triphosphate to release pyrophosphates (By similarity).By similarity

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei58SubstrateBy similarity1
Binding sitei101SubstrateBy similarity1
Binding sitei132SubstrateBy similarity1
Metal bindingi192Magnesium 1By similarity1
Metal bindingi192Magnesium 2By similarity1
Binding sitei217SubstrateBy similarity1
Metal bindingi234Magnesium 2By similarity1
Binding sitei234SubstrateBy similarity1
Metal bindingi236Magnesium 1By similarity1
Metal bindingi236Magnesium 2By similarity1
Metal bindingi253Magnesium 1By similarity1
Metal bindingi254Magnesium 1; via carbonyl oxygenBy similarity1
Binding sitei280SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Exonuclease, Hydrolase, Nuclease

Keywords - Ligandi

Magnesium, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Decapping and exoribonuclease protein (EC:3.1.13.-, EC:3.6.1.-)
Short name:
DXO
Alternative name(s):
Dom-3 homolog Z
Gene namesi
Name:DXO
Synonyms:DOM3L, DOM3Z, NG6
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:2992. DXO.

Subcellular locationi

  • Nucleus 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi1797.
OpenTargetsiENSG00000204348.
ENSG00000206346.
ENSG00000224313.
ENSG00000225682.
ENSG00000234798.
PharmGKBiPA27458.

Polymorphism and mutation databases

BioMutaiDOM3Z.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002498221 – 396Decapping and exoribonuclease proteinAdd BLAST396

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei392PhosphothreonineBy similarity1
Modified residuei394PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO77932.
MaxQBiO77932.
PaxDbiO77932.
PeptideAtlasiO77932.
PRIDEiO77932.

PTM databases

iPTMnetiO77932.
PhosphoSitePlusiO77932.

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiENSG00000204348.
CleanExiHS_DOM3Z.
ExpressionAtlasiO77932. baseline and differential.
GenevisibleiO77932. HS.

Organism-specific databases

HPAiHPA046708.

Interactioni

Protein-protein interaction databases

BioGridi108132. 15 interactors.
IntActiO77932. 8 interactors.
MINTiMINT-1034427.
STRINGi9606.ENSP00000337759.

Structurei

3D structure databases

ProteinModelPortaliO77932.
SMRiO77932.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DXO/Dom3Z family.Curated

Phylogenomic databases

eggNOGiKOG1982. Eukaryota.
ENOG410ZAFR. LUCA.
GeneTreeiENSGT00390000006425.
HOVERGENiHBG080627.
InParanoidiO77932.
KOiK14845.
OMAiHSPGQWR.
OrthoDBiEOG091G0F0X.
PhylomeDBiO77932.
TreeFamiTF322812.

Family and domain databases

InterProiIPR013961. RAI1.
[Graphical view]
PfamiPF08652. RAI1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O77932-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPRGTKRGA EKTEVAEPRN KLPRPAPSLP TDPALYSGPF PFYRRPSELG
60 70 80 90 100
CFSLDAQRQY HGDARALRYY SPPPTNGPGP NFDLRDGYPD RYQPRDEEVQ
110 120 130 140 150
ERLDHLLCWL LEHRGRLEGG PGWLAEAIVT WRGHLTKLLT TPYERQEGWQ
160 170 180 190 200
LAASRFQGTL YLSEVETPNA RAQRLARPPL LRELMYMGYK FEQYMCADKP
210 220 230 240 250
GSSPDPSGEV NTNVAFCSVL RSRLGSHPLL FSGEVDCTDP QAPSTQPPTC
260 270 280 290 300
YVELKTSKEM HSPGQWRSFY RHKLLKWWAQ SFLPGVPNVV AGFRNPDGFV
310 320 330 340 350
SSLKTFPTMK MFEYVRNDRD GWNPSVCMNF CAAFLSFAQS TVVQDDPRLV
360 370 380 390
HLFSWEPGGP VTVSVHQDAP YAFLPIWYVE AMTQDLPSPP KTPSPK
Length:396
Mass (Da):44,929
Last modified:September 19, 2006 - v2
Checksum:i17C8119037EB6892
GO

Sequence cautioni

The sequence AAB67983 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB89306 differs from that shown. Reason: Erroneous gene model prediction.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02749228S → T.2 PublicationsCorresponds to variant rs1056694dbSNPEnsembl.1
Natural variantiVAR_02749363D → E.Corresponds to variant rs2746396dbSNPEnsembl.1
Natural variantiVAR_027494261H → Q.1 PublicationCorresponds to variant rs17207867dbSNPEnsembl.1
Natural variantiVAR_027495332A → V.Corresponds to variant rs12205138dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF059252 mRNA. Translation: AAC78603.1.
AF019413 Genomic DNA. Translation: AAB67983.1. Sequence problems.
AL049547 Genomic DNA. Translation: CAB89305.1.
AL049547 Genomic DNA. Translation: CAB89306.1. Sequence problems.
AL662849 Genomic DNA. Translation: CAI17465.1.
AL844853 Genomic DNA. Translation: CAI41864.1.
CR753822 Genomic DNA. Translation: CAM26055.1.
CR759782 Genomic DNA. Translation: CAQ07122.1.
AL645922 Genomic DNA. Translation: CAQ09281.1.
CR753845 Genomic DNA. Translation: CAQ10912.1.
CH471081 Genomic DNA. Translation: EAX03558.1.
BC009344 mRNA. Translation: AAH09344.1.
BC019083 mRNA. Translation: AAH19083.1.
AF059253 mRNA. Translation: AAC78604.1.
AF059254 mRNA. Translation: AAC78605.1.
CCDSiCCDS4732.1.
RefSeqiNP_005501.2. NM_005510.3.
XP_006715068.1. XM_006715005.3.
UniGeneiHs.153299.

Genome annotation databases

EnsembliENST00000337523; ENSP00000337759; ENSG00000204348.
ENST00000375349; ENSP00000364498; ENSG00000204348.
ENST00000375356; ENSP00000364505; ENSG00000204348.
ENST00000383330; ENSP00000372820; ENSG00000206346.
ENST00000399936; ENSP00000382818; ENSG00000206346.
ENST00000399937; ENSP00000382819; ENSG00000206346.
ENST00000414092; ENSP00000391123; ENSG00000236765.
ENST00000419797; ENSP00000391532; ENSG00000234798.
ENST00000426498; ENSP00000394167; ENSG00000225682.
ENST00000427627; ENSP00000413860; ENSG00000234798.
ENST00000427690; ENSP00000406143; ENSG00000225682.
ENST00000432110; ENSP00000401315; ENSG00000236765.
ENST00000439989; ENSP00000395262; ENSG00000224313.
ENST00000443250; ENSP00000415675; ENSG00000224313.
ENST00000446967; ENSP00000391349; ENSG00000234798.
ENST00000453742; ENSP00000410404; ENSG00000225682.
ENST00000454430; ENSP00000411380; ENSG00000224313.
ENST00000458308; ENSP00000400173; ENSG00000236765.
GeneIDi1797.
KEGGihsa:1797.
UCSCiuc003nyp.2. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF059252 mRNA. Translation: AAC78603.1.
AF019413 Genomic DNA. Translation: AAB67983.1. Sequence problems.
AL049547 Genomic DNA. Translation: CAB89305.1.
AL049547 Genomic DNA. Translation: CAB89306.1. Sequence problems.
AL662849 Genomic DNA. Translation: CAI17465.1.
AL844853 Genomic DNA. Translation: CAI41864.1.
CR753822 Genomic DNA. Translation: CAM26055.1.
CR759782 Genomic DNA. Translation: CAQ07122.1.
AL645922 Genomic DNA. Translation: CAQ09281.1.
CR753845 Genomic DNA. Translation: CAQ10912.1.
CH471081 Genomic DNA. Translation: EAX03558.1.
BC009344 mRNA. Translation: AAH09344.1.
BC019083 mRNA. Translation: AAH19083.1.
AF059253 mRNA. Translation: AAC78604.1.
AF059254 mRNA. Translation: AAC78605.1.
CCDSiCCDS4732.1.
RefSeqiNP_005501.2. NM_005510.3.
XP_006715068.1. XM_006715005.3.
UniGeneiHs.153299.

3D structure databases

ProteinModelPortaliO77932.
SMRiO77932.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108132. 15 interactors.
IntActiO77932. 8 interactors.
MINTiMINT-1034427.
STRINGi9606.ENSP00000337759.

PTM databases

iPTMnetiO77932.
PhosphoSitePlusiO77932.

Polymorphism and mutation databases

BioMutaiDOM3Z.

Proteomic databases

EPDiO77932.
MaxQBiO77932.
PaxDbiO77932.
PeptideAtlasiO77932.
PRIDEiO77932.

Protocols and materials databases

DNASUi1797.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000337523; ENSP00000337759; ENSG00000204348.
ENST00000375349; ENSP00000364498; ENSG00000204348.
ENST00000375356; ENSP00000364505; ENSG00000204348.
ENST00000383330; ENSP00000372820; ENSG00000206346.
ENST00000399936; ENSP00000382818; ENSG00000206346.
ENST00000399937; ENSP00000382819; ENSG00000206346.
ENST00000414092; ENSP00000391123; ENSG00000236765.
ENST00000419797; ENSP00000391532; ENSG00000234798.
ENST00000426498; ENSP00000394167; ENSG00000225682.
ENST00000427627; ENSP00000413860; ENSG00000234798.
ENST00000427690; ENSP00000406143; ENSG00000225682.
ENST00000432110; ENSP00000401315; ENSG00000236765.
ENST00000439989; ENSP00000395262; ENSG00000224313.
ENST00000443250; ENSP00000415675; ENSG00000224313.
ENST00000446967; ENSP00000391349; ENSG00000234798.
ENST00000453742; ENSP00000410404; ENSG00000225682.
ENST00000454430; ENSP00000411380; ENSG00000224313.
ENST00000458308; ENSP00000400173; ENSG00000236765.
GeneIDi1797.
KEGGihsa:1797.
UCSCiuc003nyp.2. human.

Organism-specific databases

CTDi1797.
DisGeNETi1797.
GeneCardsiDXO.
H-InvDBHIX0166417.
HGNCiHGNC:2992. DXO.
HPAiHPA046708.
MIMi605996. gene.
neXtProtiNX_O77932.
OpenTargetsiENSG00000204348.
ENSG00000206346.
ENSG00000224313.
ENSG00000225682.
ENSG00000234798.
PharmGKBiPA27458.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1982. Eukaryota.
ENOG410ZAFR. LUCA.
GeneTreeiENSGT00390000006425.
HOVERGENiHBG080627.
InParanoidiO77932.
KOiK14845.
OMAiHSPGQWR.
OrthoDBiEOG091G0F0X.
PhylomeDBiO77932.
TreeFamiTF322812.

Miscellaneous databases

ChiTaRSiDXO. human.
GeneWikiiDOM3Z.
GenomeRNAii1797.
PROiO77932.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000204348.
CleanExiHS_DOM3Z.
ExpressionAtlasiO77932. baseline and differential.
GenevisibleiO77932. HS.

Family and domain databases

InterProiIPR013961. RAI1.
[Graphical view]
PfamiPF08652. RAI1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDXO_HUMAN
AccessioniPrimary (citable) accession number: O77932
Secondary accession number(s): A2CER3
, B0UZ80, O15004, O78127, O78128, Q5ST60, Q6IPZ2, Q9NPK4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: September 19, 2006
Last modified: November 2, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

In contrast to canonical decapping enzymes DCP2 and NUDT16, which release m7pppG (m7GDP), the decapping activity releases the entire cap structure GpppN and a 5' end monophosphate RNA.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.