Reviewed, UniProtKB/Swiss-Prot O77836 (MGT4A_BOVIN)

Names and origin

Protein names

Recommended name:
    Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A
    EC=2.4.1.145
Alternative name(s):
    UDP-N-acetylglucosamine: alpha-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IVa
    N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVa
      Short name=N-acetylglucosaminyltransferase IVa
      Short name=GlcNAc-T IVa
      Short name=GnT-IVa
Cleaved into the following chain:
    1- Recommended name:
            Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A soluble form

Gene names

Name:

MGAT4A

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	535 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N-linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans. Essential for the production of tri- and tetra-antennary N-linked sugar chains. Involved in glucose transport by mediating SLC2A2/GLUT2 glycosylation, thereby controlling cell-surface expression of SLC2A2 in pancreatic beta cells.
Catalytic activity	UDP-N-acetyl-D-glucosamine + 3-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 3-(2,4-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.
Cofactor	Divalent metal cations. Ref.2
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity. Secreted Probable. Note: A processed soluble form also exists.
Tissue specificity	Highly expressed in small intestine, kidney, lung and spleen. Weakly expressed in brain, heart and liver.
Sequence similarities	Belongs to the glycosyltransferase 54 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane Secreted
Domain	Coiled coil Signal-anchor Transmembrane
Ligand	Metal-binding
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alpha-1,3-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 535	535	Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A		PRO_0000288579
	Chain	93 – 535	443	Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A soluble form		PRO_0000288580
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 27	21	Signal-anchor for type II membrane protein Potential
	Topological domain	28 – 535	508	Lumenal Potential
	Coiled coil	31 – 57	27	Potential
Amino acid modifications
	Glycosylation	77	1	N-linked (GlcNAc...) Potential
	Glycosylation	458	1	N-linked (GlcNAc...) Ref.1
Experimental info
	Mutagenesis	79	1	S → A: Does not abolish enzyme activity. Ref.1
	Mutagenesis	460	1	T → A: Does not abolish enzyme activity. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O77836-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 614C1AA3B536BB5D	FASTA	535	61,618
	10 20 30 40 50 60 MRLRNGTVAT VLAFITSFLT LSWYTTWQNG KEKVIAYQRE FLALKERLRI AEHRISQRSS 70 80 90 100 110 120 ELSAIVQQFK RVEAETNRSK DPVNKFSDDT LKILKELTSK KSLQVPSIYY HLPHLLQNEG 130 140 150 160 170 180 SLQPAVQIGN GRTGVSIVMG IPTVKREVKS YLIETLHSLI DNLYPEEKLD CVIVVFIGET 190 200 210 220 230 240 DTDYVNGVVA NLEKEFSKEI SSGLVEIISP PESYYPDLTN LKETFGDSKE RVRWRTKQNL 250 260 270 280 290 300 DYCFLMMYAQ EKGTYYIQLE DDIIVKQNYF NTIKNFALQL SSEEWMILEF SQLGFIGKMF 310 320 330 340 350 360 QAPDLTLIVE FIFMFYKEKP IDWLLDHILW VKVCNPEKDA KHCDRQKANL RIRFRPSLFQ 370 380 390 400 410 420 HVGLHSSLTG KIQKLTDKDY MKPLLLKIHV NPPAEVSTSL KVYQGHTLEK TYMGEDFFWA 430 440 450 460 470 480 ITPVAGDYIL FKFDKPVNVE SYLFHSGNQD HPGDILLNTT VEVLPLKSEG LDISKETKDK 490 500 510 520 530 RLEDGYFRIG KFENGVAEGM VDPSLNPISA FRLSVIQNSA VWAILNEIHI KKVTN

References

[1]

"cDNA cloning and expression of bovine UDP-N-acetylglucosamine: alpha1, 3-D-mannoside beta1,4-N-acetylglucosaminyltransferase IV." Minowa M.T., Oguri S., Yoshida A., Hara T., Iwamatsu A., Ikenaga H., Takeuchi M. J. Biol. Chem. 273:11556-11562(1998) [PubMed: 9565571] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 93-96; 161-168; 183-194; 198-222; 230-251; 261-266; 326-331; 379-382; 387-425; 427-432; 434-448; 454-463; 467-475 AND 484-491, CLEAVAGE, GLYCOSYLATION AT ASN-458, MUTAGENESIS OF SER-79 AND THR-460. Tissue: Small intestine.

[2]

"Purification and characterization of UDP-N-acetylglucosamine: alpha1,3-D-mannoside beta1,4-N-acetylglucosaminyltransferase (N-acetylglucosaminyltransferase-IV) from bovine small intestine." Oguri S., Minowa M.T., Ihara Y., Taniguchi N., Ikenaga H., Takeuchi M. J. Biol. Chem. 272:22721-22727(1997) [PubMed: 9278430] [Abstract] Cited for: IDENTIFICATION, COFACTOR.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB000628 mRNA. Translation: BAA31162.1.
IPI	IPI00701535.
RefSeq	NP_803486.1.
UniGene	Bt.193
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT54. Glycosyltransferase Family 54.
Genome annotation databases
Ensembl	ENSBTAG00000010388. Bos taurus. [Contig view]
GeneID	282276.
KEGG	bta:282276.
Phylogenomic databases
HOVERGEN	O77836.
Enzyme and pathway databases
BRENDA	2.4.1.145. 251.
Family and domain databases
InterPro	IPR006759. Glyco_transf_54. [Graphical view]
PANTHER	PTHR12062. Glyco_transf_54. 1 hit.
Pfam	PF04666. Glyco_transf_54. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MGT4A_BOVIN

Accession

Primary (citable) accession number: O77836

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	November 1, 1998
Last modified:	June 16, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)