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O77836 (MGT4A_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A
EC=2.4.1.145
Alternative name(s):
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVa
Short name=GlcNAc-T IVa
Short name=GnT-IVa
Short name=N-acetylglucosaminyltransferase IVa
UDP-N-acetylglucosamine: alpha-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IVa

Cleaved into the following chain:

  1. Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A soluble form
Gene names
Name:MGAT4A
OrganismBos taurus (Bovine)
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length535 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N-linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans. Essential for the production of tri- and tetra-antennary N-linked sugar chains. Involved in glucose transport by mediating SLC2A2/GLUT2 glycosylation, thereby controlling cell-surface expression of SLC2A2 in pancreatic beta cells.

Catalytic activity

UDP-N-acetyl-D-glucosamine + 3-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 3-(2,4-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.

Cofactor

Divalent metal cations. Ref.2

Pathway

Protein modification; protein glycosylation.

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein By similarity. Secreted Probable. Note: A processed soluble form also exists.

Tissue specificity

Highly expressed in small intestine, kidney, lung and spleen. Weakly expressed in brain, heart and liver.

Sequence similarities

Belongs to the glycosyltransferase 54 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 535535Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A
PRO_0000288579
Chain93 – 535443Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A soluble form
PRO_0000288580

Regions

Topological domain1 – 66Cytoplasmic Potential
Transmembrane7 – 2721Helical; Signal-anchor for type II membrane protein; Potential
Topological domain28 – 535508Lumenal Potential
Coiled coil31 – 5727 Potential

Amino acid modifications

Glycosylation771N-linked (GlcNAc...) Potential
Glycosylation4581N-linked (GlcNAc...) Ref.1

Experimental info

Mutagenesis791S → A: Does not abolish enzyme activity. Ref.1
Mutagenesis4601T → A: Does not abolish enzyme activity. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O77836 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 614C1AA3B536BB5D

FASTA53561,618
        10         20         30         40         50         60 
MRLRNGTVAT VLAFITSFLT LSWYTTWQNG KEKVIAYQRE FLALKERLRI AEHRISQRSS 

        70         80         90        100        110        120 
ELSAIVQQFK RVEAETNRSK DPVNKFSDDT LKILKELTSK KSLQVPSIYY HLPHLLQNEG 

       130        140        150        160        170        180 
SLQPAVQIGN GRTGVSIVMG IPTVKREVKS YLIETLHSLI DNLYPEEKLD CVIVVFIGET 

       190        200        210        220        230        240 
DTDYVNGVVA NLEKEFSKEI SSGLVEIISP PESYYPDLTN LKETFGDSKE RVRWRTKQNL 

       250        260        270        280        290        300 
DYCFLMMYAQ EKGTYYIQLE DDIIVKQNYF NTIKNFALQL SSEEWMILEF SQLGFIGKMF 

       310        320        330        340        350        360 
QAPDLTLIVE FIFMFYKEKP IDWLLDHILW VKVCNPEKDA KHCDRQKANL RIRFRPSLFQ 

       370        380        390        400        410        420 
HVGLHSSLTG KIQKLTDKDY MKPLLLKIHV NPPAEVSTSL KVYQGHTLEK TYMGEDFFWA 

       430        440        450        460        470        480 
ITPVAGDYIL FKFDKPVNVE SYLFHSGNQD HPGDILLNTT VEVLPLKSEG LDISKETKDK 

       490        500        510        520        530 
RLEDGYFRIG KFENGVAEGM VDPSLNPISA FRLSVIQNSA VWAILNEIHI KKVTN

« Hide

References

[1]"cDNA cloning and expression of bovine UDP-N-acetylglucosamine: alpha1, 3-D-mannoside beta1,4-N-acetylglucosaminyltransferase IV."
Minowa M.T., Oguri S., Yoshida A., Hara T., Iwamatsu A., Ikenaga H., Takeuchi M.
J. Biol. Chem. 273:11556-11562(1998) [PubMed: 9565571] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 93-96; 161-168; 183-194; 198-222; 230-251; 261-266; 326-331; 379-382; 387-425; 427-432; 434-448; 454-463; 467-475 AND 484-491, CLEAVAGE, GLYCOSYLATION AT ASN-458, MUTAGENESIS OF SER-79 AND THR-460.
Tissue: Small intestine.
[2]"Purification and characterization of UDP-N-acetylglucosamine: alpha1,3-D-mannoside beta1,4-N-acetylglucosaminyltransferase (N-acetylglucosaminyltransferase-IV) from bovine small intestine."
Oguri S., Minowa M.T., Ihara Y., Taniguchi N., Ikenaga H., Takeuchi M.
J. Biol. Chem. 272:22721-22727(1997) [PubMed: 9278430] [Abstract]
Cited for: IDENTIFICATION, COFACTOR.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AB000628 mRNA. Translation: BAA31162.1.
IPIIPI00701535.
RefSeqNP_803486.1. NM_177520.2.
UniGeneBt.193.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGO77836.

Protein family/group databases

CAZyGT54. Glycosyltransferase Family 54.

Proteomic databases

PRIDEO77836.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID282276.
KEGGbta:282276.

Organism-specific databases

CTD11320.

Phylogenomic databases

eggNOGmaNOG08028.
GeneTreeENSGT00550000074578.
HOVERGENHBG053194.
InParanoidO77836.
OrthoDBEOG428219.

Enzyme and pathway databases

BRENDA2.4.1.145. 908.

Family and domain databases

InterProIPR006759. Glyco_transf_54.
[Graphical view]
KOK00738.
PANTHERPTHR12062. Glyco_transf_54. 1 hit.
PfamPF04666. Glyco_transf_54. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMGT4A_BOVIN
AccessionPrimary (citable) accession number: O77836
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: November 1, 1998
Last modified: November 16, 2011
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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