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Protein

Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A

Gene

MGAT4A

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N-linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans. Essential for the production of tri- and tetra-antennary N-linked sugar chains. Involved in glucose transport by mediating SLC2A2/GLUT2 glycosylation, thereby controlling cell-surface expression of SLC2A2 in pancreatic beta cells.

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 3-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 3-(2,4-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.

Cofactori

a divalent metal cation1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Metal-binding

Enzyme and pathway databases

BRENDAi2.4.1.145. 908.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT54. Glycosyltransferase Family 54.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A (EC:2.4.1.145)
Alternative name(s):
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVa
Short name:
GlcNAc-T IVa
Short name:
GnT-IVa
Short name:
N-acetylglucosaminyltransferase IVa
UDP-N-acetylglucosamine: alpha-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IVa
Cleaved into the following chain:
Gene namesi
Name:MGAT4A
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66CytoplasmicSequence analysis
Transmembranei7 – 2721Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini28 – 535508LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi79 – 791S → A: Does not abolish enzyme activity. 1 Publication
Mutagenesisi460 – 4601T → A: Does not abolish enzyme activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 535535Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase APRO_0000288579Add
BLAST
Chaini93 – 535443Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A soluble formPRO_0000288580Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi77 – 771N-linked (GlcNAc...)Sequence analysis
Glycosylationi458 – 4581N-linked (GlcNAc...)1 Publication
Modified residuei474 – 4741PhosphoserineBy similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiO77836.
PRIDEiO77836.

Expressioni

Tissue specificityi

Highly expressed in small intestine, kidney, lung and spleen. Weakly expressed in brain, heart and liver.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000013715.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili31 – 5727Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyltransferase 54 family.Curated

Keywords - Domaini

Coiled coil, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IEDC. Eukaryota.
ENOG410XPV0. LUCA.
HOGENOMiHOG000015085.
HOVERGENiHBG053194.
InParanoidiO77836.
KOiK00738.

Family and domain databases

InterProiIPR006759. Glyco_transf_54.
[Graphical view]
PANTHERiPTHR12062. PTHR12062. 1 hit.
PfamiPF04666. Glyco_transf_54. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O77836-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLRNGTVAT VLAFITSFLT LSWYTTWQNG KEKVIAYQRE FLALKERLRI
60 70 80 90 100
AEHRISQRSS ELSAIVQQFK RVEAETNRSK DPVNKFSDDT LKILKELTSK
110 120 130 140 150
KSLQVPSIYY HLPHLLQNEG SLQPAVQIGN GRTGVSIVMG IPTVKREVKS
160 170 180 190 200
YLIETLHSLI DNLYPEEKLD CVIVVFIGET DTDYVNGVVA NLEKEFSKEI
210 220 230 240 250
SSGLVEIISP PESYYPDLTN LKETFGDSKE RVRWRTKQNL DYCFLMMYAQ
260 270 280 290 300
EKGTYYIQLE DDIIVKQNYF NTIKNFALQL SSEEWMILEF SQLGFIGKMF
310 320 330 340 350
QAPDLTLIVE FIFMFYKEKP IDWLLDHILW VKVCNPEKDA KHCDRQKANL
360 370 380 390 400
RIRFRPSLFQ HVGLHSSLTG KIQKLTDKDY MKPLLLKIHV NPPAEVSTSL
410 420 430 440 450
KVYQGHTLEK TYMGEDFFWA ITPVAGDYIL FKFDKPVNVE SYLFHSGNQD
460 470 480 490 500
HPGDILLNTT VEVLPLKSEG LDISKETKDK RLEDGYFRIG KFENGVAEGM
510 520 530
VDPSLNPISA FRLSVIQNSA VWAILNEIHI KKVTN
Length:535
Mass (Da):61,618
Last modified:November 1, 1998 - v1
Checksum:i614C1AA3B536BB5D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB000628 mRNA. Translation: BAA31162.1.
RefSeqiNP_803486.1. NM_177520.3.
UniGeneiBt.193.

Genome annotation databases

GeneIDi282276.
KEGGibta:282276.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB000628 mRNA. Translation: BAA31162.1.
RefSeqiNP_803486.1. NM_177520.3.
UniGeneiBt.193.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000013715.

Protein family/group databases

CAZyiGT54. Glycosyltransferase Family 54.

Proteomic databases

PaxDbiO77836.
PRIDEiO77836.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi282276.
KEGGibta:282276.

Organism-specific databases

CTDi11320.

Phylogenomic databases

eggNOGiENOG410IEDC. Eukaryota.
ENOG410XPV0. LUCA.
HOGENOMiHOG000015085.
HOVERGENiHBG053194.
InParanoidiO77836.
KOiK00738.

Enzyme and pathway databases

UniPathwayiUPA00378.
BRENDAi2.4.1.145. 908.

Miscellaneous databases

NextBioi20806087.

Family and domain databases

InterProiIPR006759. Glyco_transf_54.
[Graphical view]
PANTHERiPTHR12062. PTHR12062. 1 hit.
PfamiPF04666. Glyco_transf_54. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "cDNA cloning and expression of bovine UDP-N-acetylglucosamine: alpha1, 3-D-mannoside beta1,4-N-acetylglucosaminyltransferase IV."
    Minowa M.T., Oguri S., Yoshida A., Hara T., Iwamatsu A., Ikenaga H., Takeuchi M.
    J. Biol. Chem. 273:11556-11562(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 93-96; 161-168; 183-194; 198-222; 230-251; 261-266; 326-331; 379-382; 387-425; 427-432; 434-448; 454-463; 467-475 AND 484-491, CLEAVAGE, GLYCOSYLATION AT ASN-458, MUTAGENESIS OF SER-79 AND THR-460.
    Tissue: Small intestine.
  2. "Purification and characterization of UDP-N-acetylglucosamine: alpha1,3-D-mannoside beta1,4-N-acetylglucosaminyltransferase (N-acetylglucosaminyltransferase-IV) from bovine small intestine."
    Oguri S., Minowa M.T., Ihara Y., Taniguchi N., Ikenaga H., Takeuchi M.
    J. Biol. Chem. 272:22721-22727(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION, COFACTOR.

Entry informationi

Entry nameiMGT4A_BOVIN
AccessioniPrimary (citable) accession number: O77836
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: November 1, 1998
Last modified: February 17, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.