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Protein

Acyl-protein thioesterase 1

Gene

LYPLA1

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. Has depalmitoylating activity toward KCNMA1. Has low lysophospholipase activity (By similarity).By similarity

Catalytic activityi

Palmitoyl-protein + H2O = palmitate + protein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei119 – 1191Charge relay systemBy similarity
Active sitei174 – 1741Charge relay systemBy similarity
Active sitei208 – 2081Charge relay systemBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Protein family/group databases

ESTHERirabit-lypla1. LYsophospholipase_carboxylesterase.

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl-protein thioesterase 1 (EC:3.1.2.-)
Short name:
APT-1
Alternative name(s):
Calcium-independent phospholipase A2
Short name:
CaIPLA2
Lysophospholipase 1
Lysophospholipase I
Short name:
LPL-I
Short name:
LysoPLA I
Gene namesi
Name:LYPLA1
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
Proteomesi
  • UP000001811 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 230230Acyl-protein thioesterase 1PRO_0000102269Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei79 – 791PhosphoserineBy similarity
Modified residuei224 – 2241N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiO77821.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliO77821.
SMRiO77821. Positions 6-229.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000260139.
HOVERGENiHBG052378.
InParanoidiO77821.
KOiK06128.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR003140. PLipase/COase/thioEstase.
[Graphical view]
PfamiPF02230. Abhydrolase_2. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequencei

Sequence statusi: Complete.

O77821-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGNNMSAPM PAVVPAARKA TAAVIFLHGL GDTGHGWAEA FAGIKSPHIK
60 70 80 90 100
YICPHAPVMP VTLNMNMAMP SWFDIVGLSP DSQEDESGIK QAAETVKALI
110 120 130 140 150
DQEVKNGIPS NRIILGGFSQ GGALSLYTAL TTQQKLAGVT ALSCWLPLRA
160 170 180 190 200
SFSQGPINSA NRDISVLQCH GDCDPLVPLM FGSLTVERLK ALINPANVTF
210 220 230
KIYEGMMHSS CQQEMMDVKH FIDKLLPPID
Length:230
Mass (Da):24,688
Last modified:November 1, 1998 - v1
Checksum:i89AF2017AEFC9FAC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U97148 mRNA. Translation: AAC63432.1.
RefSeqiNP_001103287.1. NM_001109817.1.
UniGeneiOcu.2303.

Genome annotation databases

GeneIDi100125993.
KEGGiocu:100125993.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U97148 mRNA. Translation: AAC63432.1.
RefSeqiNP_001103287.1. NM_001109817.1.
UniGeneiOcu.2303.

3D structure databases

ProteinModelPortaliO77821.
SMRiO77821. Positions 6-229.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

ESTHERirabit-lypla1. LYsophospholipase_carboxylesterase.

Proteomic databases

PRIDEiO77821.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100125993.
KEGGiocu:100125993.

Organism-specific databases

CTDi10434.

Phylogenomic databases

HOGENOMiHOG000260139.
HOVERGENiHBG052378.
InParanoidiO77821.
KOiK06128.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR003140. PLipase/COase/thioEstase.
[Graphical view]
PfamiPF02230. Abhydrolase_2. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "cDNA cloning and expression of a novel family of enzymes with calcium-independent phospholipase A2 and lysophospholipase activities."
    Portilla D., Crew M.D., Grant D., Serrero G., Bates L.M., Dai G., Sasner M., Cheng J., Buonanno A.
    J. Am. Soc. Nephrol. 9:1178-1186(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 98-103; 150-161 AND 191-201.
    Tissue: Kidney.

Entry informationi

Entry nameiLYPA1_RABIT
AccessioniPrimary (citable) accession number: O77821
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: November 1, 1998
Last modified: July 6, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.