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O77814

- KCRS_RABIT

UniProt

O77814 - KCRS_RABIT

Protein

Creatine kinase S-type, mitochondrial

Gene

CKMT2

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa By similarity.By similarity

    Catalytic activityi

    ATP + creatine = ADP + phosphocreatine.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei225 – 2251ATPPROSITE-ProRule annotation
    Binding sitei270 – 2701ATPPROSITE-ProRule annotation
    Binding sitei326 – 3261ATPPROSITE-ProRule annotation
    Binding sitei369 – 3691ATPPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi162 – 1665ATPPROSITE-ProRule annotation
    Nucleotide bindingi354 – 3596ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. creatine kinase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Creatine kinase S-type, mitochondrial (EC:2.7.3.2)
    Alternative name(s):
    Basic-type mitochondrial creatine kinase
    Short name:
    Mib-CK
    RSMTCK
    Sarcomeric mitochondrial creatine kinase
    Short name:
    S-MtCK
    Gene namesi
    Name:CKMT2
    OrganismiOryctolagus cuniculus (Rabbit)
    Taxonomic identifieri9986 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
    ProteomesiUP000001811: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial inner membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3939MitochondrionAdd
    BLAST
    Chaini40 – 419380Creatine kinase S-type, mitochondrialPRO_0000016597Add
    BLAST

    Proteomic databases

    PRIDEiO77814.

    Interactioni

    Subunit structurei

    Exists as an octamer composed of four CKMT2 homodimers.By similarity

    Protein-protein interaction databases

    STRINGi9986.ENSOCUP00000009646.

    Structurei

    3D structure databases

    ProteinModelPortaliO77814.
    SMRiO77814. Positions 47-413.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini46 – 13287Phosphagen kinase N-terminalPROSITE-ProRule annotationAdd
    BLAST
    Domaini159 – 401243Phosphagen kinase C-terminalPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni40 – 6425Cardiolipin-bindingBy similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ATP:guanido phosphotransferase family.PROSITE-ProRule annotation
    Contains 1 phosphagen kinase C-terminal domain.PROSITE-ProRule annotation
    Contains 1 phosphagen kinase N-terminal domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG3869.
    HOGENOMiHOG000232165.
    HOVERGENiHBG001339.

    Family and domain databases

    Gene3Di1.10.135.10. 1 hit.
    3.30.590.10. 1 hit.
    InterProiIPR022415. ATP-guanido_PTrfase_AS.
    IPR022414. ATP-guanido_PTrfase_cat.
    IPR022413. ATP-guanido_PTrfase_N.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    [Graphical view]
    PfamiPF00217. ATP-gua_Ptrans. 1 hit.
    PF02807. ATP-gua_PtransN. 1 hit.
    [Graphical view]
    SUPFAMiSSF48034. SSF48034. 1 hit.
    PROSITEiPS00112. PHOSPHAGEN_KINASE. 1 hit.
    PS51510. PHOSPHAGEN_KINASE_C. 1 hit.
    PS51509. PHOSPHAGEN_KINASE_N. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O77814-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASTFSKLLT GRNASLLFAT LGTSALTTGY LVNRQKVCAE ARDQHKLFPP    50
    SADYPDLRKH NNCMAECLTP SIYAKLRNKV TANGYTLDQC IQTGVDNPGH 100
    PFIKTVGMVA GDEESYEVFA DLFDPVIKLR HNGYDPRVMK HPTDLDASKI 150
    TQGQFDERYV LSSRVRTGRS IRGLSLPPAC SRAEAREVEN VAITALEGLK 200
    GDLAGRYYRL SEMTEQDQQR LIDDHFLFDK PVSPLLTCAG MARDWPDARG 250
    IWHNYDNTFL IWINEEDHTR VISMEKGGNM KRVFERFCRG LKEVERLIQE 300
    RGWEFMWNER LGYILTCPSN LGTGLRAGVH VRIPKLSKDP RFSKILENLR 350
    LQKRGTGGVD TRAVADVYDI SNIDRIGRSE VELVQIVIDG VNYLVDCEKK 400
    LERGQDIKVP PPLPQFGKK 419
    Length:419
    Mass (Da):47,407
    Last modified:November 1, 1998 - v1
    Checksum:iC26469B25730CC6F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ011334 mRNA. Translation: CAA09597.1.
    RefSeqiNP_001156542.1. NM_001163070.1.
    UniGeneiOcu.3234.

    Genome annotation databases

    GeneIDi100302412.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ011334 mRNA. Translation: CAA09597.1 .
    RefSeqi NP_001156542.1. NM_001163070.1.
    UniGenei Ocu.3234.

    3D structure databases

    ProteinModelPortali O77814.
    SMRi O77814. Positions 47-413.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9986.ENSOCUP00000009646.

    Proteomic databases

    PRIDEi O77814.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100302412.

    Organism-specific databases

    CTDi 1160.

    Phylogenomic databases

    eggNOGi COG3869.
    HOGENOMi HOG000232165.
    HOVERGENi HBG001339.

    Family and domain databases

    Gene3Di 1.10.135.10. 1 hit.
    3.30.590.10. 1 hit.
    InterProi IPR022415. ATP-guanido_PTrfase_AS.
    IPR022414. ATP-guanido_PTrfase_cat.
    IPR022413. ATP-guanido_PTrfase_N.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    [Graphical view ]
    Pfami PF00217. ATP-gua_Ptrans. 1 hit.
    PF02807. ATP-gua_PtransN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48034. SSF48034. 1 hit.
    PROSITEi PS00112. PHOSPHAGEN_KINASE. 1 hit.
    PS51510. PHOSPHAGEN_KINASE_C. 1 hit.
    PS51509. PHOSPHAGEN_KINASE_N. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, Escherichia coli expression, and phase-transition chromatography-based purification of recombinant rabbit heart mitochondrial creatine kinase."
      Marcillat O., Perraut C., Granjon T., Vial C., Vacheron M.J.
      Protein Expr. Purif. 17:163-168(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Heart.

    Entry informationi

    Entry nameiKCRS_RABIT
    AccessioniPrimary (citable) accession number: O77814
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Mitochondrial creatine kinase binds cardiolipin.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3