true2005-04-122024-03-27110CP1A2_MACFAMolecular cloning and functional analysis of cynomolgus monkey CYP1A2.Sakuma T.Hieda M.Igarashi T.Ohgiya S.Nagata R.Nemoto N.Kamataki T.doi:10.1016/s0006-2952(98)00100-21998Biochem. Pharmacol.56131-139NUCLEOTIDE SEQUENCE [MRNA]INDUCTIONCHARACTERIZATIONLiver1 site, No reported glycansCYP1ACytochrome P450Cyt_P450Cyt_P450_CSCyt_P450_E_grp-ICyt_P450_E_grp-I_CYP1Cyt_P450_sfCYTOCHROME P450 1A2STEROID 17-ALPHA-HYDROXYLASE/17,20 LYASEp450EP450IEP450ICYP1AP450Cytochrome P450CYTOCHROME_P450Cytochrome P450 1A21.14.14.1CYPIA2Cholesterol 25-hydroxylaseHydroperoxy icosatetraenoate dehydratase4.2.1.152CYP1A2A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2. Metabolizes cholesterol toward 25-hydroxycholesterol, a physiological regulator of cellular cholesterol homeostasis. May act as a major enzyme for all-trans retinoic acid biosynthesis in the liver. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid. Primarily catalyzes stereoselective epoxidation of the last double bond of polyunsaturated fatty acids (PUFA), displaying a strong preference for the (R,S) stereoisomer. Catalyzes bisallylic hydroxylation and omega-1 hydroxylation of PUFA. May also participate in eicosanoids metabolism by converting hydroperoxide species into oxo metabolites (lipoxygenase-like reaction, NADPH-independent). Plays a role in the oxidative metabolism of xenobiotics. Catalyzes the N-hydroxylation of heterocyclic amines and the O-deethylation of phenacetin. Metabolizes caffeine via N3-demethylation.Interacts with PGRMC1; the interaction requires PGRMC1 homodimerization.Liver.By 3-methylcholanthrene (3MC).Belongs to the cytochrome P450 family.Cytochrome P450 1A2581971516226axial binding residue458O-linked (GlcNAc) serine69substrate2007-01-233581979b6ad740c2b6cec1843b706a8c18f403MALSQSVPFLATELLLASAIFCLVFWVLRGSRPRVPKGLKSPPEPWGWPLLGHVLTLGKNPHLALSRMSQLYGDVLQIRIGSTPVLVLSGLDTIRQALVRQGDDFKGRPDLYSFTFITDGQSMSFSPDSGPVWAARRRLAQNALNTFSIASDPASSSSCYLEEHVSKEAEALISRLQELMAGPGHFDPYNQVVVSVANVIGAMCFGQHFPESSDEMLSLVKNSHEFVESASSGNPVDFFPILRYLPNPALQRFKAFNQRFRRFLQKTVQEHYQDFDKNSVQDITGALFKHSKKGPRASGNLIPQEKIVNLVNDIFGAGFDTIATAISWSLMYLVTKPEIQRKIQKELDAVIGRGRRPRLSDRPQLPYLEAFILETFRHSSFVPFTIPHSTTRDTTLNGFYIPRECCVFINQWQVNHDPQLWGDPSEFRPERFLTAEGTTINKPLSEKIMLFGLGKRRCIGEVLGKWEVFLFLAILLQQLEFSVPPGVKVDLTPIYGLTMKHARCEHFQARLRFSIKtruetruetruetruetrue