Reviewed, UniProtKB/Swiss-Prot O77809 (CP1A2_MACFA)

Names and origin

Protein names

Recommended name:
    Cytochrome P450 1A2
    EC=1.14.14.1
Alternative name(s):
    CYPIA2

Gene names

Name:

CYP1A2

Organism

Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	516 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Tissue specificity	Liver.
Induction	By 3-methylcholanthrene (3MC). Ref.1
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
PTM	Acetylation
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 516	515	Cytochrome P450 1A2		PRO_0000051652
Sites
	Metal binding	458	1	Iron (heme axial ligand) By similarity
Amino acid modifications
	Modified residue	289	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O77809-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 3F71E3999EC4CDF7	FASTA	516	58,197
	10 20 30 40 50 60 MALSQSVPFL ATELLLASAI FCLVFWVLRG SRPRVPKGLK SPPEPWGWPL LGHVLTLGKN 70 80 90 100 110 120 PHLALSRMSQ LYGDVLQIRI GSTPVLVLSG LDTIRQALVR QGDDFKGRPD LYSFTFITDG 130 140 150 160 170 180 QSMSFSPDSG PVWAARRRLA QNALNTFSIA SDPASSSSCY LEEHVSKEAE ALISRLQELM 190 200 210 220 230 240 AGPGHFDPYN QVVVSVANVI GAMCFGQHFP ESSDEMLSLV KNSHEFVESA SSGNPVDFFP 250 260 270 280 290 300 ILRYLPNPAL QRFKAFNQRF RRFLQKTVQE HYQDFDKNSV QDITGALFKH SKKGPRASGN 310 320 330 340 350 360 LIPQEKIVNL VNDIFGAGFD TIATAISWSL MYLVTKPEIQ RKIQKELDAV IGRGRRPRLS 370 380 390 400 410 420 DRPQLPYLEA FILETFRHSS FVPFTIPHST TRDTTLNGFY IPRECCVFIN QWQVNHDPQL 430 440 450 460 470 480 WGDPSEFRPE RFLTAEGTTI NKPLSEKIML FGLGKRRCIG EVLGKWEVFL FLAILLQQLE 490 500 510 FSVPPGVKVD LTPIYGLTMK HARCEHFQAR LRFSIK

References

[1]

"Molecular cloning and functional analysis of cynomolgus monkey CYP1A2." Sakuma T., Hieda M., Igarashi T., Ohgiya S., Nagata R., Nemoto N., Kamataki T. Biochem. Pharmacol. 56:131-139(1998) [PubMed: 9698097] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, CHARACTERIZATION. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D86474 mRNA. Translation: BAA33789.1.
3D structure databases
HSSP	HSSP built from PDB template 1DT6 based on UniProtKB P00179.
SMR	O77809. Positions 34-514.
ModBase	Search...
Phylogenomic databases
HOVERGEN	O77809.
Enzyme and pathway databases
BRENDA	1.14.14.1. 3438.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. IPR008066. Cyt_P450_E_grp-I_CYP1. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit. PTHR19383:SF63. Cyt_P450_E_grp-I_CYP1. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR01683. EP450ICYP1A. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP1A2_MACFA

Accession

Primary (citable) accession number: O77809

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 12, 2005
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 54 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)