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O77793

- PA24A_HORSE

UniProt

O77793 - PA24A_HORSE

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Protein

Cytosolic phospholipase A2

Gene

PLA2G4A

Organism
Equus caballus (Horse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response.

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.
2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

Enzyme regulationi

Stimulated by agonists such as ATP, EGF, thrombin and bradykinin as well as by cytosolic Ca2+.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi40 – 401Calcium 1By similarity
Metal bindingi40 – 401Calcium 2By similarity
Metal bindingi41 – 411Calcium 1; via carbonyl oxygenBy similarity
Metal bindingi43 – 431Calcium 1By similarity
Metal bindingi43 – 431Calcium 2By similarity
Metal bindingi65 – 651Calcium 1By similarity
Metal bindingi93 – 931Calcium 2By similarity
Metal bindingi94 – 941Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi95 – 951Calcium 2By similarity
Active sitei228 – 2281NucleophileBy similarity
Active sitei549 – 5491Proton acceptorBy similarity

GO - Molecular functioni

  1. calcium-dependent phospholipid binding Source: UniProtKB
  2. calcium ion binding Source: UniProtKB
  3. lysophospholipase activity Source: UniProtKB-EC
  4. phospholipase A2 activity Source: UniProtKB

GO - Biological processi

  1. phospholipid catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.1.1.4. 2120.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic phospholipase A2
Short name:
cPLA2
Alternative name(s):
Phospholipase A2 group IVA
Including the following 2 domains:
Phospholipase A2 (EC:3.1.1.4)
Alternative name(s):
Phosphatidylcholine 2-acylhydrolase
Lysophospholipase (EC:3.1.1.5)
Gene namesi
Name:PLA2G4A
Synonyms:CPLA2, PLA2G4
OrganismiEquus caballus (Horse)
Taxonomic identifieri9796 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaPerissodactylaEquidaeEquus
ProteomesiUP000002281: Unplaced

Subcellular locationi

Cytoplasm By similarity. Cytoplasmic vesicle By similarity
Note: Translocates to membrane vesicles in a calcium-dependent fashion.By similarity

GO - Cellular componenti

  1. cytoplasmic vesicle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 749749Cytosolic phospholipase A2PRO_0000187261Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei268 – 2681PhosphothreonineBy similarity
Modified residuei437 – 4371PhosphoserineBy similarity
Modified residuei505 – 5051Phosphoserine; by MAPKBy similarity
Modified residuei727 – 7271PhosphoserineBy similarity
Modified residuei729 – 7291PhosphoserineBy similarity

Post-translational modificationi

Activated by phosphorylation at both Ser-505 and Ser-727.By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi9796.ENSECAP00000022445.

Structurei

3D structure databases

ProteinModelPortaliO77793.
SMRiO77793. Positions 13-721.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 106102C2PROSITE-ProRule annotationAdd
BLAST
Domaini140 – 740601PLA2cPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 178178Phospholipid bindingCuratedAdd
BLAST

Domaini

The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca2+ (By similarity).By similarity

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 1 PLA2c domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG257248.
HOGENOMiHOG000115420.
HOVERGENiHBG053479.
InParanoidiO77793.
KOiK16342.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR000008. C2_dom.
IPR002642. LysoPLipase_cat_dom.
[Graphical view]
PfamiPF00168. C2. 1 hit.
PF01735. PLA2_B. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 1 hit.
SM00022. PLAc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF52151. SSF52151. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS51210. PLA2C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O77793-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSFIDPYQHI IVEHQYSHKF TVVVLRATKV TKGAFGDMLD TPDPYVELFI
60 70 80 90 100
SSTPDSRKRT RHFNNNINPV WNETFEFILD PNQENVLEIT LMDANYVMDE
110 120 130 140 150
TLGTATFTLS SMKVGEKKEV PFIFNQVTEM ILEMSLEVCS CPDLRFSMAL
160 170 180 190 200
CDQEKTFRQQ RKENIKENMK KLLGPKKSEG LYSTRDVPVV AILGSGGGFR
210 220 230 240 250
AMVGFSGVMK ALYESGILDC ATYLAGLSGS SWYMSTLYSH PDFPEKGPEE
260 270 280 290 300
INKELMKNVS YDPLLLLTPQ KIKRYVESLW KKKSSGQPVT FTDIFGMLIG
310 320 330 340 350
ETLIHNRMNT TLSSLKEKVN TAQCPLPLFT CLHVKPDVSE LMFADWVEFS
360 370 380 390 400
PYEIGMAKYG TFMAPDLFGS KFFMGTVVKK YEENPLHFLM GVWGSAFSIL
410 420 430 440 450
FNRVLGVSGS QNKGSTMEEE LENITAKHIV SNDSSDSDDE SQEPKGTENE
460 470 480 490 500
DAERDYQNDN QASWVHRMLM ALVSDSALFN TREGRAGKVH NFMLGLNLNT
510 520 530 540 550
SYPLSPLRNF TTQESLDDDE LDAAVADPDE FERIYEPLDV KSKKIHVVDS
560 570 580 590 600
GLTFNLPYPL ILRPQRGVDL IISFDFSARP SDSSPPFKEL LLAEKWAKMN
610 620 630 640 650
KLPFPKIDPY VFDREGLKEC YVFKPKNPDV EKDCPTIIHF VLANINFRKY
660 670 680 690 700
KAPGVPRETK EEKEIADFDI FDDPESPFST FNFQYPNQAF KRLHDLMHFN
710 720 730 740
TLNNIDVIKN AMVESIEYRR QNPSRCSVSL SNVEARRFFN KEFLNKPTA
Length:749
Mass (Da):85,454
Last modified:November 1, 1998 - v1
Checksum:i493A67DF28D50FE0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF092539 mRNA. Translation: AAC61774.1.
RefSeqiNP_001075312.1. NM_001081843.1.
UniGeneiEca.12988.

Genome annotation databases

GeneIDi100033889.
KEGGiecb:100033889.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF092539 mRNA. Translation: AAC61774.1 .
RefSeqi NP_001075312.1. NM_001081843.1.
UniGenei Eca.12988.

3D structure databases

ProteinModelPortali O77793.
SMRi O77793. Positions 13-721.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9796.ENSECAP00000022445.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100033889.
KEGGi ecb:100033889.

Organism-specific databases

CTDi 5321.

Phylogenomic databases

eggNOGi NOG257248.
HOGENOMi HOG000115420.
HOVERGENi HBG053479.
InParanoidi O77793.
KOi K16342.

Enzyme and pathway databases

BRENDAi 3.1.1.4. 2120.

Family and domain databases

Gene3Di 2.60.40.150. 1 hit.
InterProi IPR016035. Acyl_Trfase/lysoPLipase.
IPR000008. C2_dom.
IPR002642. LysoPLipase_cat_dom.
[Graphical view ]
Pfami PF00168. C2. 1 hit.
PF01735. PLA2_B. 1 hit.
[Graphical view ]
SMARTi SM00239. C2. 1 hit.
SM00022. PLAc. 1 hit.
[Graphical view ]
SUPFAMi SSF49562. SSF49562. 1 hit.
SSF52151. SSF52151. 1 hit.
PROSITEi PS50004. C2. 1 hit.
PS51210. PLA2C. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Purification, characterization and cDNA sequencing of calcium-dependent cytosolic phospholipase A2 from equine neutrophils."
    Larsson Forsell P.K.A., Lindberg A., Karlsson S., Lindgren J.A., Claesson H.-E.
    Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Neutrophil.

Entry informationi

Entry nameiPA24A_HORSE
AccessioniPrimary (citable) accession number: O77793
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1998
Last modified: October 29, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3