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O77788 (NFM_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Neurofilament medium polypeptide
Short name=NF-M
Alternative name(s):
160 kDa neurofilament protein
Neurofilament 3
Neurofilament triplet M protein
Gene names
Name:NEFM
Synonyms:NEF3, NFM
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length926 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.

Post-translational modification

Phosphorylated on a number of serine residues in the repeated K-S-P tripeptide motif. Phosphorylation of NFH may result in the formation of interfilament cross-links that are important in the maintenance of axonal caliber By similarity. Ref.1

Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function By similarity.

Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization By similarity. Ref.1

Sequence similarities

Belongs to the intermediate filament family.

Mass spectrometry

Molecular mass is 105044 Da from positions 2 - 926. Determined by MALDI. Ref.1

Ontologies

Keywords
   Cellular componentIntermediate filament
   DomainCoiled coil
Repeat
   PTMAcetylation
Glycoprotein
Phosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Cellular_componentintermediate filament

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionstructural molecule activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.1
Chain2 – 926925Neurofilament medium polypeptide
PRO_0000063793

Regions

Repeat512 – 51651
Repeat619 – 62352
Repeat624 – 62853
Repeat629 – 63354
Repeat634 – 63855
Repeat639 – 64356
Repeat644 – 64857
Repeat649 – 65358
Repeat654 – 65859
Repeat659 – 663510
Repeat664 – 668511
Repeat669 – 673512
Repeat674 – 678513
Repeat679 – 683514
Repeat684 – 688515
Repeat689 – 693516
Repeat694 – 698517
Region2 – 412411Rod
Region105 – 13632Coil 1A
Region137 – 14913Linker 1
Region150 – 24899Coil 1B
Region249 – 26517Linker 12
Region266 – 28722Coil 2A
Region288 – 2914Linker 2
Region292 – 412121Coil 2B
Region413 – 926514Tail
Region512 – 69818717 X 5 AA approximate tandem repeats of K-S-P-[TVEA]-[AKETP]

Amino acid modifications

Modified residue21N-acetylserine Ref.1
Modified residue3201Phosphotyrosine By similarity
Modified residue5131Phosphoserine Ref.1
Modified residue5471Phosphoserine Ref.1
Modified residue5551Phosphoserine Ref.1
Modified residue5611Phosphoserine Ref.1
Modified residue6281Phosphothreonine Ref.1
Modified residue6301Phosphoserine Ref.1
Modified residue6351Phosphoserine Ref.1
Modified residue6401Phosphoserine Ref.1
Modified residue6471Phosphothreonine Ref.1
Modified residue6501Phosphoserine Ref.1
Modified residue6551Phosphoserine Ref.1
Modified residue6651Phosphoserine Ref.1
Modified residue6701Phosphoserine Ref.1
Modified residue6771Phosphothreonine Ref.1
Modified residue6801Phosphoserine Ref.1
Modified residue6851Phosphoserine Ref.1
Modified residue6901Phosphoserine By similarity
Modified residue6951Phosphoserine Ref.1
Modified residue7271Phosphoserine Ref.1
Modified residue7511Phosphoserine Ref.1
Modified residue7571Phosphoserine Ref.1
Modified residue7711Phosphoserine Ref.1
Modified residue8471Phosphoserine Ref.1
Glycosylation471O-linked (GlcNAc) By similarity

Sequences

Sequence LengthMass (Da)Tools
O77788 [UniParc].

Last modified April 3, 2007. Version 3.
Checksum: B1C2FA96E31793C0

FASTA926103,210
        10         20         30         40         50         60 
MSYTLDSLGN PSAYRRVTET RSSFSRISGS PSSGFRSQSW SRGSPSTVSS SYKRSALAPR 

        70         80         90        100        110        120 
LTYSSAMLSS AESSLDFSQS SSLLDGGSGP GGDYKLSRSN EKEQIQGLND RFAGYIEKVH 

       130        140        150        160        170        180 
YLEQQNKEIE AEIQALRQKQ ASHAQLGDAY DQEIRELRAT LEMVNHEKAQ VQLDSDHLEE 

       190        200        210        220        230        240 
DIHRLKERFE EEARLRDDTE AAIRALRKDI EESSLVKVEL DKKVQSLQDE VAFLRSNHEE 

       250        260        270        280        290        300 
EVADLLAQIQ ASHITVERKD YLKTDISTAL KEIRSQLESH SDQNMHQAEE WFKCRYAKLT 

       310        320        330        340        350        360 
EAAEQNKEAI RSAKEEIAEY RRQLQSKSIE LESVRGTKES LERQLSDIEE RHNHDLSSYQ 

       370        380        390        400        410        420 
DTIQQLENEL RGTKWEMARH LREYQDLLNV KMALDIEIAA YRKLLEGEET RFSTFAGSIT 

       430        440        450        460        470        480 
GPLYTHRQPS IAISSKIQKT KVEAPKLKVQ HKFVEEIIEE TKVEDEKSEM EEALTAITEE 

       490        500        510        520        530        540 
LAVSVKEEVK EEEAEEKEEK EEAEEEVVAA KKSPVKATAP ELKEEEGEKE EEEGQEEEEE 

       550        560        570        580        590        600 
EEEAAKSDQA EEGGSEKEGS SEKEEGEQEE EGETEAEGEG EEAAAEAKEE KKMEEKAEEV 

       610        620        630        640        650        660 
APKEELAAEA KVEKPEKAKS PVAKSPTTKS PTAKSPEAKS PEAKSPTAKS PTAKSPVAKS 

       670        680        690        700        710        720 
PTAKSPEAKS PEAKSPTAKS PTAKSPAAKS PAPKSPVEEV KPKAEAGAEK GEQKEKVEEE 

       730        740        750        760        770        780 
KKEAKESPKE EKAEKKEEKP KDVPEKKKAE SPVKAESPVK EEVPAKPVKV SPEKEAKEEE 

       790        800        810        820        830        840 
KPQEKEKEKE KVEEVGGKEE GGLKESRKED IAINGEVEGK EEEQETKEKG SGGEEEKGVV 

       850        860        870        880        890        900 
TNGLDVSPGD EKKGGDKSEE KVVVTKMVEK ITSEGGDGAT KYITKSVTVT QKVEEHEETF 

       910        920 
EEKLVSTKKV EKVTSHAIVK EVTQSD

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References

[1]"Identification of endogenous phosphorylation sites of bovine medium and low molecular weight neurofilament proteins by tandem mass spectrometry."
Trimpin S., Mixon A.E., Stapels M.D., Kim M.Y., Spencer P.S., Deinzer M.L.
Biochemistry 43:2091-2105(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-116, ACETYLATION AT SER-2, PHOSPHORYLATION AT SER-513; SER-547; SER-555; SER-561; THR-628; SER-630; SER-635; SER-640; THR-647; SER-650; SER-655; SER-665; SER-670; THR-677; SER-680; SER-685; SER-695; SER-727; SER-751; SER-757; SER-771 AND SER-847, MASS SPECTROMETRY.
[2]"The bovine neurofilament M subunit has a novel set of KSP repeats normally restricted to NF-H."
Hill W.D., Zhang L., Balin B.J., Sprinkle T.J., Spicer K., Gearhart D.A.
Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 117-926.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF091342 mRNA. Translation: AAC36357.1.
IPIIPI00705032.
UniGeneBt.51690.

3D structure databases

ProteinModelPortalO77788.
SMRO77788. Positions 99-136, 330-407.
ModBaseSearch...

Protein-protein interaction databases

IntActO77788. 1 interaction.
STRING9913.ENSBTAP00000052505.

Proteomic databases

PaxDbO77788.
PRIDEO77788.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGNOG264891.
HOGENOMHOG000230977.
HOVERGENHBG013015.
InParanoidO77788.
OrthoDBEOG4VMFFD.

Family and domain databases

InterProIPR016044. F.
IPR001664. IF.
IPR006821. Intermed_filament_DNA-bd.
IPR018039. Intermediate_filament_CS.
IPR002957. Keratin_I.
[Graphical view]
PANTHERPTHR23239. PTHR23239. 1 hit.
PfamPF00038. Filament. 1 hit.
PF04732. Filament_head. 1 hit.
[Graphical view]
PRINTSPR01248. TYPE1KERATIN.
PROSITEPS00226. IF. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNFM_BOVIN
AccessionPrimary (citable) accession number: O77788
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: April 3, 2007
Last modified: April 3, 2013
This is version 91 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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