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Protein

Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial

Gene

IDH3B

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Plays a structural role to facilitate the assembly and ensure the full activity of the enzyme catalyzing the decarboxylation of isocitrate (ICT) into alpha-ketoglutarate. The heterodimer composed of the alpha (IDH3A) and beta (IDH3B) subunits and the heterodimer composed of the alpha (IDH3A) and gamma (IDH3G) subunits, have considerable basal activity but the full activity of the heterotetramer (containing two subunits of IDH3A, one of IDH3B and one of IDH3G) requires the assembly and cooperative function of both heterodimers.By similarity

Enzyme regulationi

The heterotetramer and the heterodimer composed of IDH3A and IDH3G subunits can be allosterically activated by citrate (CIT) or/and ADP, and the two activators can act independently or synergistically. The heterodimer composed of IDH3A and IDH3B subunits cannot be allosterically regulated and the allosteric regulation of the heterotetramer is through the IDH3G subunit and not the IDH3B subunit. The IDH3G subunit contains the allosteric site which consists of a CIT-binding site and an ADP-binding site, and the binding of CIT and ADP causes conformational changes at the allosteric site which are transmitted to the active site in the catalytic subunit (IDH3A) through a cascade of conformational changes at the heterodimer interface, leading to stabilization of the isocitrate-binding at the active site and thus activation of the enzyme. ATP can activate the heterotetramer and the heterodimer composed of IDH3A and IDH3G subunits at low concentrations but inhibits their activities at high concentrations, whereas ATP exhibits only inhibitory effect on the heterodimer composed of IDH3A and IDH3B subunits.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processTricarboxylic acid cycle

Enzyme and pathway databases

ReactomeiR-BTA-71403. Citric acid cycle (TCA cycle).

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial
Alternative name(s):
Isocitric dehydrogenase subunit beta
NAD(+)-isocitrate dehydrogenase subunit 1
Short name:
IDH1
NAD(+)-specific ICDH subunit beta
Gene namesi
Name:IDH3B
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 13

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 33MitochondrionBy similarityAdd BLAST33
ChainiPRO_000001444334 – 385Isocitrate dehydrogenase [NAD] subunit beta, mitochondrialAdd BLAST352

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei199N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiO77784.
PRIDEiO77784.

Expressioni

Tissue specificityi

Isoform A is predominant in heart muscle; also found in brain, kidney and liver. Isoform B is present in kidney and liver.

Gene expression databases

BgeeiENSBTAG00000018813.

Interactioni

Subunit structurei

Heterooligomer of subunits alpha (IDH3A), beta (IDH3B), and gamma (IDH3G) in the apparent ratio of 2:1:1. The heterodimer containing one IDH3A and one IDH3B subunit and the heterodimer containing one IDH3A and one IDH3G subunit assemble into a heterotetramer (which contains two subunits of IDH3A, one of IDH3B and one of IDH3G) and further into the heterooctamer.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000025044.

Structurei

3D structure databases

ProteinModelPortaliO77784.
SMRiO77784.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0784. Eukaryota.
COG0473. LUCA.
GeneTreeiENSGT00590000083091.
HOGENOMiHOG000021113.
HOVERGENiHBG052080.
InParanoidiO77784.
KOiK00030.
OMAiIVPGCNM.
OrthoDBiEOG091G094X.
TreeFamiTF315033.

Family and domain databases

InterProiView protein in InterPro
IPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004434. Isocitrate_DH_NAD.
IPR024084. IsoPropMal-DH-like_dom.
PfamiView protein in Pfam
PF00180. Iso_dh. 1 hit.
SMARTiView protein in SMART
SM01329. Iso_dh. 1 hit.
TIGRFAMsiTIGR00175. mito_nad_idh. 1 hit.
PROSITEiView protein in PROSITE
PS00470. IDH_IMDH. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform B (identifier: O77784-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAALSRVRWL TRALVAAPNP GAWRSLCTST VAQASSRTQG EDVRVEGAFP
60 70 80 90 100
VTMLPGDGVG PELMHAVKEV FKAASVPVEF QEHHLSEVQN MASEEKLEQV
110 120 130 140 150
LSSMKENKVA IIGKIHTPME YKGELASYDM RLRRKLDLFA NVVHVKSLPG
160 170 180 190 200
YKTRHNNLDL VIIREQTEGE YSSLEHESAR GVIECLKIVT RTKSQRIAKF
210 220 230 240 250
AFDYATKKGR GKVTAVHKAN IMKLGDGLFL QCCEEVAELY PKIKFEKMII
260 270 280 290 300
DNCCMQLVQN PYQFDVLVMP NLYGNIIDNL AAGLVGGAGV VPGESYSAEY
310 320 330 340 350
AVFETGARHP FAQAVGRNIA NPTAMLLSAS NMLRHLNLEH HSNMIAEAVK
360 370 380
KVIKVGKVRT RDMGGYSTTT DFIKSVIGHL HPYGG
Length:385
Mass (Da):42,497
Last modified:July 15, 1999 - v2
Checksum:i8E974AD550F5D50D
GO
Isoform A (identifier: O77784-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     361-385: RDMGGYSTTTDFIKSVIGHLHPYGG → SDMGGYATCQDFTEAVIGALSNP

Show »
Length:383
Mass (Da):42,135
Checksum:i2F20288CD939ECC9
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_002461361 – 385RDMGG…HPYGG → SDMGGYATCQDFTEAVIGAL SNP in isoform A. 1 PublicationAdd BLAST25

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF090321 mRNA. Translation: AAC83166.1.
AF090322 mRNA. Translation: AAC83167.1.
BC104502 mRNA. Translation: AAI04503.1.
PIRiS58432.
RefSeqiNP_001139344.1. NM_001145872.1. [O77784-1]
NP_001161346.1. NM_001167874.1. [O77784-2]
UniGeneiBt.56156.

Genome annotation databases

EnsembliENSBTAT00000025044; ENSBTAP00000025044; ENSBTAG00000018813. [O77784-1]
ENSBTAT00000045446; ENSBTAP00000042833; ENSBTAG00000018813. [O77784-1]
GeneIDi613338.
KEGGibta:613338.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiIDH3B_BOVIN
AccessioniPrimary (citable) accession number: O77784
Secondary accession number(s): O77785, Q3MI01
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: July 15, 1999
Last modified: September 27, 2017
This is version 126 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families