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O77784

- IDH3B_BOVIN

UniProt

O77784 - IDH3B_BOVIN

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Protein
Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial
Gene
IDH3B
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH.

Cofactori

Binds 1 magnesium or manganese ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei133 – 1331Substrate By similarity
Binding sitei164 – 1641Substrate By similarity
Sitei171 – 1711Critical for catalysis By similarity
Sitei218 – 2181Critical for catalysis By similarity
Metal bindingi251 – 2511Magnesium or manganese By similarity
Binding sitei251 – 2511Substrate By similarity

GO - Molecular functioni

  1. NAD binding Source: InterPro
  2. isocitrate dehydrogenase (NAD+) activity Source: UniProtKB-EC
  3. magnesium ion binding Source: InterPro

GO - Biological processi

  1. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, NAD

Enzyme and pathway databases

ReactomeiREACT_227707. Citric acid cycle (TCA cycle).

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial (EC:1.1.1.41)
Alternative name(s):
Isocitric dehydrogenase subunit beta
NAD(+)-isocitrate dehydrogenase subunit 1
Short name:
IDH1
NAD(+)-specific ICDH subunit beta
Gene namesi
Name:IDH3B
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 13

Subcellular locationi

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3333Mitochondrion By similarity
Add
BLAST
Chaini34 – 385352Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial
PRO_0000014443Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei199 – 1991N6-acetyllysine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiO77784.

Expressioni

Tissue specificityi

Isoform A is predominant in heart muscle; also found in brain, kidney and liver. Isoform B is present in kidney and liver.

Interactioni

Subunit structurei

Heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1 By similarity.

Structurei

3D structure databases

ProteinModelPortaliO77784.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0473.
GeneTreeiENSGT00590000083091.
HOGENOMiHOG000021113.
HOVERGENiHBG052080.
InParanoidiO77784.
KOiK00030.
OMAiILATPDY.
OrthoDBiEOG75B85R.
TreeFamiTF315033.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR004434. Isocitrate_DH_NAD.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11835. PTHR11835. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00175. mito_nad_idh. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform B (identifier: O77784-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MAALSRVRWL TRALVAAPNP GAWRSLCTST VAQASSRTQG EDVRVEGAFP    50
VTMLPGDGVG PELMHAVKEV FKAASVPVEF QEHHLSEVQN MASEEKLEQV 100
LSSMKENKVA IIGKIHTPME YKGELASYDM RLRRKLDLFA NVVHVKSLPG 150
YKTRHNNLDL VIIREQTEGE YSSLEHESAR GVIECLKIVT RTKSQRIAKF 200
AFDYATKKGR GKVTAVHKAN IMKLGDGLFL QCCEEVAELY PKIKFEKMII 250
DNCCMQLVQN PYQFDVLVMP NLYGNIIDNL AAGLVGGAGV VPGESYSAEY 300
AVFETGARHP FAQAVGRNIA NPTAMLLSAS NMLRHLNLEH HSNMIAEAVK 350
KVIKVGKVRT RDMGGYSTTT DFIKSVIGHL HPYGG 385
Length:385
Mass (Da):42,497
Last modified:July 15, 1999 - v2
Checksum:i8E974AD550F5D50D
GO
Isoform A (identifier: O77784-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     361-385: RDMGGYSTTTDFIKSVIGHLHPYGG → SDMGGYATCQDFTEAVIGALSNP

Show »
Length:383
Mass (Da):42,135
Checksum:i2F20288CD939ECC9
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei361 – 38525RDMGG…HPYGG → SDMGGYATCQDFTEAVIGAL SNP in isoform A.
VSP_002461Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF090321 mRNA. Translation: AAC83166.1.
AF090322 mRNA. Translation: AAC83167.1.
BC104502 mRNA. Translation: AAI04503.1.
PIRiS58432.
RefSeqiNP_001139344.1. NM_001145872.1. [O77784-1]
NP_001161346.1. NM_001167874.1. [O77784-2]
UniGeneiBt.56156.

Genome annotation databases

EnsembliENSBTAT00000025044; ENSBTAP00000025044; ENSBTAG00000018813. [O77784-1]
ENSBTAT00000045446; ENSBTAP00000042833; ENSBTAG00000018813. [O77784-1]
GeneIDi613338.
KEGGibta:613338.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF090321 mRNA. Translation: AAC83166.1 .
AF090322 mRNA. Translation: AAC83167.1 .
BC104502 mRNA. Translation: AAI04503.1 .
PIRi S58432.
RefSeqi NP_001139344.1. NM_001145872.1. [O77784-1 ]
NP_001161346.1. NM_001167874.1. [O77784-2 ]
UniGenei Bt.56156.

3D structure databases

ProteinModelPortali O77784.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi O77784.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000025044 ; ENSBTAP00000025044 ; ENSBTAG00000018813 . [O77784-1 ]
ENSBTAT00000045446 ; ENSBTAP00000042833 ; ENSBTAG00000018813 . [O77784-1 ]
GeneIDi 613338.
KEGGi bta:613338.

Organism-specific databases

CTDi 3420.

Phylogenomic databases

eggNOGi COG0473.
GeneTreei ENSGT00590000083091.
HOGENOMi HOG000021113.
HOVERGENi HBG052080.
InParanoidi O77784.
KOi K00030.
OMAi ILATPDY.
OrthoDBi EOG75B85R.
TreeFami TF315033.

Enzyme and pathway databases

Reactomei REACT_227707. Citric acid cycle (TCA cycle).

Miscellaneous databases

NextBioi 20898527.

Family and domain databases

Gene3Di 3.40.718.10. 1 hit.
InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR004434. Isocitrate_DH_NAD.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view ]
PANTHERi PTHR11835. PTHR11835. 1 hit.
Pfami PF00180. Iso_dh. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00175. mito_nad_idh. 1 hit.
PROSITEi PS00470. IDH_IMDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Bovine NAD+-dependent isocitrate dehydrogenase: alternative splicing and tissue-dependent expression of subunit 1."
    Weiss C., Zeng Y., Huang J., Sobocka M.B., Rushbrook J.I.
    Biochemistry 39:1807-1816(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND B).
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-385 (ISOFORM B).
    Strain: Hereford.
    Tissue: Ascending colon.

Entry informationi

Entry nameiIDH3B_BOVIN
AccessioniPrimary (citable) accession number: O77784
Secondary accession number(s): O77785, Q3MI01
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: July 15, 1999
Last modified: September 3, 2014
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3