Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial

Gene

IDH3B

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg(2+) or Mn2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei133 – 1331SubstrateBy similarity
Binding sitei164 – 1641SubstrateBy similarity
Sitei171 – 1711Critical for catalysisBy similarity
Sitei218 – 2181Critical for catalysisBy similarity
Metal bindingi251 – 2511Magnesium or manganeseBy similarity
Binding sitei251 – 2511SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, NAD

Enzyme and pathway databases

ReactomeiREACT_347675. Citric acid cycle (TCA cycle).

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial (EC:1.1.1.41)
Alternative name(s):
Isocitric dehydrogenase subunit beta
NAD(+)-isocitrate dehydrogenase subunit 1
Short name:
IDH1
NAD(+)-specific ICDH subunit beta
Gene namesi
Name:IDH3B
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Chromosome 13

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3333MitochondrionBy similarityAdd
BLAST
Chaini34 – 385352Isocitrate dehydrogenase [NAD] subunit beta, mitochondrialPRO_0000014443Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei199 – 1991N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiO77784.

Expressioni

Tissue specificityi

Isoform A is predominant in heart muscle; also found in brain, kidney and liver. Isoform B is present in kidney and liver.

Gene expression databases

ExpressionAtlasiO77784. baseline.

Interactioni

Subunit structurei

Heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1.By similarity

Structurei

3D structure databases

ProteinModelPortaliO77784.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0473.
GeneTreeiENSGT00590000083091.
HOGENOMiHOG000021113.
HOVERGENiHBG052080.
InParanoidiO77784.
KOiK00030.
OMAiREVAKHY.
OrthoDBiEOG75B85R.
TreeFamiTF315033.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR004434. Isocitrate_DH_NAD.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11835. PTHR11835. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00175. mito_nad_idh. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform B (identifier: O77784-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAALSRVRWL TRALVAAPNP GAWRSLCTST VAQASSRTQG EDVRVEGAFP
60 70 80 90 100
VTMLPGDGVG PELMHAVKEV FKAASVPVEF QEHHLSEVQN MASEEKLEQV
110 120 130 140 150
LSSMKENKVA IIGKIHTPME YKGELASYDM RLRRKLDLFA NVVHVKSLPG
160 170 180 190 200
YKTRHNNLDL VIIREQTEGE YSSLEHESAR GVIECLKIVT RTKSQRIAKF
210 220 230 240 250
AFDYATKKGR GKVTAVHKAN IMKLGDGLFL QCCEEVAELY PKIKFEKMII
260 270 280 290 300
DNCCMQLVQN PYQFDVLVMP NLYGNIIDNL AAGLVGGAGV VPGESYSAEY
310 320 330 340 350
AVFETGARHP FAQAVGRNIA NPTAMLLSAS NMLRHLNLEH HSNMIAEAVK
360 370 380
KVIKVGKVRT RDMGGYSTTT DFIKSVIGHL HPYGG
Length:385
Mass (Da):42,497
Last modified:July 15, 1999 - v2
Checksum:i8E974AD550F5D50D
GO
Isoform A (identifier: O77784-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     361-385: RDMGGYSTTTDFIKSVIGHLHPYGG → SDMGGYATCQDFTEAVIGALSNP

Show »
Length:383
Mass (Da):42,135
Checksum:i2F20288CD939ECC9
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei361 – 38525RDMGG…HPYGG → SDMGGYATCQDFTEAVIGAL SNP in isoform A. 1 PublicationVSP_002461Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF090321 mRNA. Translation: AAC83166.1.
AF090322 mRNA. Translation: AAC83167.1.
BC104502 mRNA. Translation: AAI04503.1.
PIRiS58432.
RefSeqiNP_001139344.1. NM_001145872.1. [O77784-1]
NP_001161346.1. NM_001167874.1. [O77784-2]
UniGeneiBt.56156.

Genome annotation databases

EnsembliENSBTAT00000025044; ENSBTAP00000025044; ENSBTAG00000018813. [O77784-1]
ENSBTAT00000045446; ENSBTAP00000042833; ENSBTAG00000018813. [O77784-1]
GeneIDi613338.
KEGGibta:613338.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF090321 mRNA. Translation: AAC83166.1.
AF090322 mRNA. Translation: AAC83167.1.
BC104502 mRNA. Translation: AAI04503.1.
PIRiS58432.
RefSeqiNP_001139344.1. NM_001145872.1. [O77784-1]
NP_001161346.1. NM_001167874.1. [O77784-2]
UniGeneiBt.56156.

3D structure databases

ProteinModelPortaliO77784.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiO77784.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000025044; ENSBTAP00000025044; ENSBTAG00000018813. [O77784-1]
ENSBTAT00000045446; ENSBTAP00000042833; ENSBTAG00000018813. [O77784-1]
GeneIDi613338.
KEGGibta:613338.

Organism-specific databases

CTDi3420.

Phylogenomic databases

eggNOGiCOG0473.
GeneTreeiENSGT00590000083091.
HOGENOMiHOG000021113.
HOVERGENiHBG052080.
InParanoidiO77784.
KOiK00030.
OMAiREVAKHY.
OrthoDBiEOG75B85R.
TreeFamiTF315033.

Enzyme and pathway databases

ReactomeiREACT_347675. Citric acid cycle (TCA cycle).

Miscellaneous databases

NextBioi20898527.

Gene expression databases

ExpressionAtlasiO77784. baseline.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR004434. Isocitrate_DH_NAD.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11835. PTHR11835. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00175. mito_nad_idh. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Bovine NAD+-dependent isocitrate dehydrogenase: alternative splicing and tissue-dependent expression of subunit 1."
    Weiss C., Zeng Y., Huang J., Sobocka M.B., Rushbrook J.I.
    Biochemistry 39:1807-1816(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND B).
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-385 (ISOFORM B).
    Strain: Hereford.
    Tissue: Ascending colon.

Entry informationi

Entry nameiIDH3B_BOVIN
AccessioniPrimary (citable) accession number: O77784
Secondary accession number(s): O77785, Q3MI01
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: July 15, 1999
Last modified: May 27, 2015
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.