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O77784

- IDH3B_BOVIN

UniProt

O77784 - IDH3B_BOVIN

Protein

Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial

Gene

IDH3B

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 2 (15 Jul 1999)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH.

    Cofactori

    Binds 1 magnesium or manganese ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei133 – 1331SubstrateBy similarity
    Binding sitei164 – 1641SubstrateBy similarity
    Sitei171 – 1711Critical for catalysisBy similarity
    Sitei218 – 2181Critical for catalysisBy similarity
    Metal bindingi251 – 2511Magnesium or manganeseBy similarity
    Binding sitei251 – 2511SubstrateBy similarity

    GO - Molecular functioni

    1. isocitrate dehydrogenase (NAD+) activity Source: UniProtKB-EC
    2. magnesium ion binding Source: InterPro
    3. NAD binding Source: InterPro

    GO - Biological processi

    1. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding, NAD

    Enzyme and pathway databases

    ReactomeiREACT_227707. Citric acid cycle (TCA cycle).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial (EC:1.1.1.41)
    Alternative name(s):
    Isocitric dehydrogenase subunit beta
    NAD(+)-isocitrate dehydrogenase subunit 1
    Short name:
    IDH1
    NAD(+)-specific ICDH subunit beta
    Gene namesi
    Name:IDH3B
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 13

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3333MitochondrionBy similarityAdd
    BLAST
    Chaini34 – 385352Isocitrate dehydrogenase [NAD] subunit beta, mitochondrialPRO_0000014443Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei199 – 1991N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PRIDEiO77784.

    Expressioni

    Tissue specificityi

    Isoform A is predominant in heart muscle; also found in brain, kidney and liver. Isoform B is present in kidney and liver.

    Interactioni

    Subunit structurei

    Heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliO77784.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0473.
    GeneTreeiENSGT00590000083091.
    HOGENOMiHOG000021113.
    HOVERGENiHBG052080.
    InParanoidiO77784.
    KOiK00030.
    OMAiILATPDY.
    OrthoDBiEOG75B85R.
    TreeFamiTF315033.

    Family and domain databases

    Gene3Di3.40.718.10. 1 hit.
    InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR001804. Isocitrate/isopropylmalate_DH.
    IPR004434. Isocitrate_DH_NAD.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view]
    PANTHERiPTHR11835. PTHR11835. 1 hit.
    PfamiPF00180. Iso_dh. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00175. mito_nad_idh. 1 hit.
    PROSITEiPS00470. IDH_IMDH. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform B (identifier: O77784-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAALSRVRWL TRALVAAPNP GAWRSLCTST VAQASSRTQG EDVRVEGAFP    50
    VTMLPGDGVG PELMHAVKEV FKAASVPVEF QEHHLSEVQN MASEEKLEQV 100
    LSSMKENKVA IIGKIHTPME YKGELASYDM RLRRKLDLFA NVVHVKSLPG 150
    YKTRHNNLDL VIIREQTEGE YSSLEHESAR GVIECLKIVT RTKSQRIAKF 200
    AFDYATKKGR GKVTAVHKAN IMKLGDGLFL QCCEEVAELY PKIKFEKMII 250
    DNCCMQLVQN PYQFDVLVMP NLYGNIIDNL AAGLVGGAGV VPGESYSAEY 300
    AVFETGARHP FAQAVGRNIA NPTAMLLSAS NMLRHLNLEH HSNMIAEAVK 350
    KVIKVGKVRT RDMGGYSTTT DFIKSVIGHL HPYGG 385
    Length:385
    Mass (Da):42,497
    Last modified:July 15, 1999 - v2
    Checksum:i8E974AD550F5D50D
    GO
    Isoform A (identifier: O77784-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         361-385: RDMGGYSTTTDFIKSVIGHLHPYGG → SDMGGYATCQDFTEAVIGALSNP

    Show »
    Length:383
    Mass (Da):42,135
    Checksum:i2F20288CD939ECC9
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei361 – 38525RDMGG…HPYGG → SDMGGYATCQDFTEAVIGAL SNP in isoform A. 1 PublicationVSP_002461Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF090321 mRNA. Translation: AAC83166.1.
    AF090322 mRNA. Translation: AAC83167.1.
    BC104502 mRNA. Translation: AAI04503.1.
    PIRiS58432.
    RefSeqiNP_001139344.1. NM_001145872.1. [O77784-1]
    NP_001161346.1. NM_001167874.1. [O77784-2]
    UniGeneiBt.56156.

    Genome annotation databases

    EnsembliENSBTAT00000025044; ENSBTAP00000025044; ENSBTAG00000018813. [O77784-1]
    ENSBTAT00000045446; ENSBTAP00000042833; ENSBTAG00000018813. [O77784-1]
    GeneIDi613338.
    KEGGibta:613338.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF090321 mRNA. Translation: AAC83166.1 .
    AF090322 mRNA. Translation: AAC83167.1 .
    BC104502 mRNA. Translation: AAI04503.1 .
    PIRi S58432.
    RefSeqi NP_001139344.1. NM_001145872.1. [O77784-1 ]
    NP_001161346.1. NM_001167874.1. [O77784-2 ]
    UniGenei Bt.56156.

    3D structure databases

    ProteinModelPortali O77784.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi O77784.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000025044 ; ENSBTAP00000025044 ; ENSBTAG00000018813 . [O77784-1 ]
    ENSBTAT00000045446 ; ENSBTAP00000042833 ; ENSBTAG00000018813 . [O77784-1 ]
    GeneIDi 613338.
    KEGGi bta:613338.

    Organism-specific databases

    CTDi 3420.

    Phylogenomic databases

    eggNOGi COG0473.
    GeneTreei ENSGT00590000083091.
    HOGENOMi HOG000021113.
    HOVERGENi HBG052080.
    InParanoidi O77784.
    KOi K00030.
    OMAi ILATPDY.
    OrthoDBi EOG75B85R.
    TreeFami TF315033.

    Enzyme and pathway databases

    Reactomei REACT_227707. Citric acid cycle (TCA cycle).

    Miscellaneous databases

    NextBioi 20898527.

    Family and domain databases

    Gene3Di 3.40.718.10. 1 hit.
    InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR001804. Isocitrate/isopropylmalate_DH.
    IPR004434. Isocitrate_DH_NAD.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view ]
    PANTHERi PTHR11835. PTHR11835. 1 hit.
    Pfami PF00180. Iso_dh. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00175. mito_nad_idh. 1 hit.
    PROSITEi PS00470. IDH_IMDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Bovine NAD+-dependent isocitrate dehydrogenase: alternative splicing and tissue-dependent expression of subunit 1."
      Weiss C., Zeng Y., Huang J., Sobocka M.B., Rushbrook J.I.
      Biochemistry 39:1807-1816(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND B).
    2. NIH - Mammalian Gene Collection (MGC) project
      Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-385 (ISOFORM B).
      Strain: Hereford.
      Tissue: Ascending colon.

    Entry informationi

    Entry nameiIDH3B_BOVIN
    AccessioniPrimary (citable) accession number: O77784
    Secondary accession number(s): O77785, Q3MI01
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: July 15, 1999
    Last modified: October 1, 2014
    This is version 105 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3