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O77783

- EXT2_BOVIN

UniProt

O77783 - EXT2_BOVIN

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Protein
Exostosin-2
Gene
EXT2
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Glycosyltransferase required for the biosynthesis of heparan-sulfate and responsible for the alternating addition of beta-1-4-linked glucuronic acid (GlcA) and alpha-1-4-linked N-acetylglucosamine (GlcNAc) units to nascent heparan sulfate chains.

Catalytic activityi

UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan = UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan.
UDP-alpha-D-glucuronate + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan = UDP + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan.

Pathwayi

GO - Molecular functioni

  1. N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase activity Source: UniProtKB
  2. acetylglucosaminyltransferase activity Source: BHF-UCL
  3. glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase activity Source: UniProtKB
  4. glucuronosyltransferase activity Source: BHF-UCL

GO - Biological processi

  1. N-acetylglucosamine metabolic process Source: UniProtKB
  2. glycosaminoglycan biosynthetic process Source: InterPro
  3. heparan sulfate proteoglycan biosynthetic process Source: InterPro
  4. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT47. Glycosyltransferase Family 47.
GT64. Glycosyltransferase Family 64.

Names & Taxonomyi

Protein namesi
Recommended name:
Exostosin-2 (EC:2.4.1.224, EC:2.4.1.225)
Alternative name(s):
Glucuronosyl-N-acetylglucosaminyl-proteoglycan/N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase
HS-polymerase
Short name:
HS-POL
Multiple exostoses protein 2 homolog
Gene namesi
Name:EXT2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

Subcellular locationi

Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity. Golgi apparatus membrane; Single-pass type II membrane protein By similarity. Secreted
Note: The EXT1/EXT2 complex is localized in the Golgi apparatus By similarity. A soluble form is found in the serum.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2525Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei26 – 4621Helical; Signal-anchor for type II membrane protein; Reviewed prediction
Add
BLAST
Topological domaini47 – 718672Lumenal Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. Golgi membrane Source: UniProtKB-SubCell
  2. extracellular space Source: BHF-UCL
  3. integral component of membrane Source: UniProtKB
  4. intrinsic component of endoplasmic reticulum membrane Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 718718Exostosin-2
PRO_0000149650Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi288 – 2881N-linked (GlcNAc...) Reviewed prediction
Glycosylationi637 – 6371N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

The soluble form derives from the membrane form by proteolytic processing.

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiO77783.

Interactioni

Subunit structurei

Forms a homo/hetero-oligomeric complex with EXT1. Interacts with GALNT5 By similarity.

Structurei

3D structure databases

ProteinModelPortaliO77783.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG272619.
HOGENOMiHOG000266990.
HOVERGENiHBG101211.
InParanoidiO77783.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR004263. Exostosin.
IPR027673. Exostosin-2.
IPR015338. HexNAc_Trfase_a.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR11062. PTHR11062. 1 hit.
PTHR11062:SF6. PTHR11062:SF6. 1 hit.
PfamiPF03016. Exostosin. 1 hit.
PF09258. Glyco_transf_64. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

O77783-1 [UniParc]FASTAAdd to Basket

« Hide

MCASVKYNIR GPALIPRMKT KHRIYYITLF SIVLLGLIAT GMFQFWPHSI    50
ESSGDWSVEK RTGRDVPLVR LPADSPVPER GDLSCRMHTC FDVYRCGFNP 100
KNKIKVYIYP LKKYVGEAGV PVSSTISREY NELLTAISDS DYYTDDVTRA 150
CLFVPSIDLL NQNSLRVKET AQALAQLSRW DRGTNHLLFN MLPGGPPDYN 200
TALDVPRDRA LLAGGGFSTW TYRQGYDVSI PVYSPLSAEV DLPEKGPGPR 250
RYFLLSSQVA LHPEYREDLA ALQARHGEAV LVLDKCSNLS EGVPAARRRC 300
HQQQAFDYPQ VLQEATFCMV LRGARLGQAV LSDVLRAGCV PVIIADSYVL 350
PFSEVLDWKR ASVVVPEEKM SDVYSILQSI PRRQIEEMQR QARWFWEAYF 400
QSIKAIALAT LQIINDRIYP YAAISYEDWN DPPAVKWGSV SNPLFLPLIP 450
PQSQGFTAIV LTYDRVESLF RVITEVSKVP SLSKLLVVWN NQNKNPPEDS 500
LWPKIRVPLK VVRTAENKLS NRFFPYDEIE TEAVLAIDDD IIMLTSDELQ 550
FGYEVWREFP DRLVGYPGRL HLWDHEMNKW KYESEWTNEV SMVLTGAAFY 600
HKYFNYLYTY KMPGDIKNWV DAHMNCEDIA MNFLVANVTG KAVIKVTPRK 650
KFKCPECTAI DGLSLDQTHM VERSECINKF ASVFGTMPLK VVEHRADPVL 700
YKDDFPEKLK SFPNIGSL 718
Length:718
Mass (Da):81,887
Last modified:November 1, 1998 - v1
Checksum:iD6C18AC9C7AAD971
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF089748 mRNA. Translation: AAC35386.1.
UniGeneiBt.5113.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF089748 mRNA. Translation: AAC35386.1 .
UniGenei Bt.5113.

3D structure databases

ProteinModelPortali O77783.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GT47. Glycosyltransferase Family 47.
GT64. Glycosyltransferase Family 64.

Proteomic databases

PRIDEi O77783.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi NOG272619.
HOGENOMi HOG000266990.
HOVERGENi HBG101211.
InParanoidi O77783.

Enzyme and pathway databases

UniPathwayi UPA00378 .

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
InterProi IPR004263. Exostosin.
IPR027673. Exostosin-2.
IPR015338. HexNAc_Trfase_a.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view ]
PANTHERi PTHR11062. PTHR11062. 1 hit.
PTHR11062:SF6. PTHR11062:SF6. 1 hit.
Pfami PF03016. Exostosin. 1 hit.
PF09258. Glyco_transf_64. 1 hit.
[Graphical view ]
SUPFAMi SSF53448. SSF53448. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The putative tumor suppressors EXT1 and EXT2 are glycosyltransferases required for the biosynthesis of heparan sulfate."
    Lind T., Tufaro F., McCormick C., Lindahl U., Lidholt K.
    J. Biol. Chem. 273:26265-26268(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 129-147; 167-179; 485-494 AND 570-577.
    Tissue: Lung.

Entry informationi

Entry nameiEXT2_BOVIN
AccessioniPrimary (citable) accession number: O77783
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: November 1, 1998
Last modified: June 11, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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