Reviewed,
UniProtKB/Swiss-Prot O77783 (EXT2_BOVIN)
Last modified
April 14, 2009.
Version 57.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Exostosin-2 EC=2.4.1.224 EC=2.4.1.225 Alternative name(s): Glucuronosyl-N-acetylglucosaminyl-proteoglycan/N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase HS-polymerase Short name=HS-POL Multiple exostoses protein 2 homolog | ||
| Gene names |
| ||
| Organism | Bos taurus (Bovine) | ||
| Taxonomic identifier | 9913 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Protein attributes
| Sequence length | 718 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Glycosyltransferase required for the biosynthesis of heparan-sulfate and responsible for the alternating addition of beta-1-4-linked glucuronic acid (GlcA) and alpha-1-4-linked N-acetylglucosamine (GlcNAc) units to nascent heparan sulfate chains. |
| Catalytic activity | UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan = UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan. UDP-alpha-D-glucuronate + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan = UDP + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan. |
| Pathway | |
| Subunit structure | Forms a homo/hetero-oligomeric complex with EXT1. Interacts with GALNT5 By similarity. |
| Subcellular location | Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity. Golgi apparatus membrane; Single-pass type II membrane protein By similarity. Secreted. Note: The EXT1/EXT2 complex is localized in the Golgi apparatus By similarity. A soluble form is found in the serum. |
| Post-translational modification | The soluble form derives from the membrane form by proteolytic processing. |
| Sequence similarities | Belongs to the glycosyltransferase 47 family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Endoplasmic reticulum Golgi apparatus Membrane Secreted |
| Domain | Signal-anchor Transmembrane |
| Molecular function | Glycosyltransferase Transferase |
| PTM | Glycoprotein |
| Technical term | Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | N-acetylglucosamine metabolic process Ref.1 Inferred from direct assay. Source: UniProtKB |
| Cellular component | Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular space Ref.1Inferred from direct assay. Source: UniProtKB integral to membrane Ref.1Non-traceable author statement. Source: UniProtKB intrinsic to endoplasmic reticulum membraneInferred from electronic annotation. Source: InterPro |
| Molecular function | N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase activity Ref.1 Inferred from direct assay. Source: UniProtKB glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase activity Ref.1Inferred from direct assay. Source: UniProtKB |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 718 | 718 | Exostosin-2 | PRO_0000149650 | |||||
Regions | |||||||||
| Topological domain | 1 – 25 | 25 | Cytoplasmic Potential | ||||||
| Transmembrane | 26 – 46 | 21 | Signal-anchor for type II membrane protein Potential | ||||||
| Topological domain | 47 – 718 | 672 | Lumenal Potential | ||||||
Amino acid modifications | |||||||||
| Glycosylation | 288 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 637 | 1 | N-linked (GlcNAc...) Potential | ||||||
Sequences
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References
| [1] | "The putative tumor suppressors EXT1 and EXT2 are glycosyltransferases required for the biosynthesis of heparan sulfate." Lind T., Tufaro F., McCormick C., Lindahl U., Lidholt K. J. Biol. Chem. 273:26265-26268(1998) [PubMed: 9756849] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 129-147; 167-179; 485-494 AND 570-577. Tissue: Lung. |
Cross-references
Sequence databases | |
|---|---|
| AF089748 mRNA. Translation: AAC35386.1. | |
| IPI | IPI00702531. |
| UniGene | Bt.5113 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1OMX based on UniProtKB Q9ES89. |
| ModBase | Search... |
Protein family/group databases | |
| CAZy | GT47. Glycosyltransferase Family 47. GT64. Glycosyltransferase Family 64. |
Genome annotation databases | |
| Ensembl | ENSBTAG00000019644. Bos taurus. [Contig view] |
Phylogenomic databases | |
| HOVERGEN | O77783. |
Enzyme and pathway databases | |
| BRENDA | 2.4.1.224. 251. 2.4.1.225. 251. |
Family and domain databases | |
| InterPro | IPR004263. Exostosin. IPR015338. HexNAc_Trfase_a. [Graphical view] |
| Pfam | PF03016. Exostosin. 1 hit. PF09258. Glyco_transf_64. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | EXT2_BOVIN | ||||||||
| Accession | Primary (citable) accession number: O77783 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


