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Protein

Disintegrin and metalloproteinase domain-containing protein 2

Gene

ADAM2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. Could have a direct role in sperm-zona binding or migration of sperm from the uterus into the oviduct. Interactions with egg membrane could be mediated via binding between its disintegrin-like domain to one or more integrins receptors on the egg. This is a non catalytic metalloprotease-like protein (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCell adhesion

Protein family/group databases

MEROPSiM12.950.

Names & Taxonomyi

Protein namesi
Recommended name:
Disintegrin and metalloproteinase domain-containing protein 2
Short name:
ADAM 2
Alternative name(s):
Fertilin subunit beta
PH-30
Short name:
PH30
PH30-beta
Gene namesi
Name:ADAM2
Synonyms:FTNB
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini17 – 696ExtracellularSequence analysisAdd BLAST680
Transmembranei697 – 717HelicalSequence analysisAdd BLAST21
Topological domaini718 – 745CytoplasmicSequence analysisAdd BLAST28

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 16Sequence analysisAdd BLAST16
PropeptideiPRO_000002903817 – 174By similarityAdd BLAST158
ChainiPRO_0000029039175 – 745Disintegrin and metalloproteinase domain-containing protein 2Add BLAST571

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi122N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi220N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi287 ↔ 370By similarity
Disulfide bondi329 ↔ 354By similarity
Disulfide bondi331 ↔ 336By similarity
Glycosylationi353N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi444 ↔ 457Sequence analysis
Glycosylationi458N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi558N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi615 ↔ 626By similarity
Disulfide bondi620 ↔ 632By similarity
Disulfide bondi634 ↔ 643By similarity
Modified residuei739PhosphoserineBy similarity1

Post-translational modificationi

The signal and the metalloprotease domain are cleaved during the epididymal maturation of the spermatozoa.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEiO77780.

Expressioni

Tissue specificityi

Expressed specifically in testis.

Interactioni

Subunit structurei

Heterodimer with ADAM1/fertilin subunit alpha.

Structurei

3D structure databases

ProteinModelPortaliO77780.
SMRiO77780.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini178 – 375Peptidase M12BPROSITE-ProRule annotationAdd BLAST198
Domaini383 – 472DisintegrinPROSITE-ProRule annotationAdd BLAST90
Domaini611 – 644EGF-likePROSITE-ProRule annotationAdd BLAST34

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi148 – 151Poly-Ser4
Compositional biasi475 – 605Cys-richAdd BLAST131

Domaini

A tripeptide motif (TDE) within disintegrin-like domain could be involved in the binding to egg integrin receptor and thus could mediate sperm/egg binding.By similarity

Keywords - Domaini

EGF-like domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000230883.
HOVERGENiHBG103628.
InParanoidiO77780.
KOiK06833.

Family and domain databases

CDDicd04269. ZnMc_adamalysin_II_like. 1 hit.
Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiView protein in InterPro
IPR033958. ADAM2.
IPR006586. ADAM_Cys-rich.
IPR018358. Disintegrin_CS.
IPR001762. Disintegrin_dom.
IPR036436. Disintegrin_dom_sf.
IPR000742. EGF-like_dom.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
IPR034027. Reprolysin_adamalysin.
PANTHERiPTHR11905:SF108. PTHR11905:SF108. 1 hit.
PfamiView protein in Pfam
PF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
PRINTSiPR00289. DISINTEGRIN.
SMARTiView protein in SMART
SM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiView protein in PROSITE
PS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS50026. EGF_3. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O77780-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLCLLFLLLG LTGLQTDDNS ERLRVQFTVP EKIRSTSSGG VETHVSYIIL
60 70 80 90 100
IEGKTYTVNL MQKAFLPHNF RVYGYSGTGS MKPLEHEFQN FCYYQGYIEG
110 120 130 140 150
YPNSMAIIST CTGLRGLLQF ENVSYGIEPL EPSIGFEHMV YQIKPRDSSS
160 170 180 190 200
SVYTEREIEL REKPYKIQNV EPLPDFSQYI EMHIVVEKDL YNHMGADTTV
210 220 230 240 250
VIQKIFQLTG LTNAIFTSLN ITVILSSLEL WIDENKIPVT GDANELLHRF
260 270 280 290 300
VKWKRSYLVL RPHDMAFLLV YREKSNYIGA TFQGRMCDKH YGGGVALHSS
310 320 330 340 350
TISLESLAVI IAQLLSLSMG IPYDDINKCH CPGDVCIMNP AAVHSSGVKL
360 370 380 390 400
FSNCSVEDFL RFISKPKSQC LQNQPRLDPT YKSAVCGNGK VEEGEQCDCG
410 420 430 440 450
NKKACDALPD TCCVADTCRF QPGSACDTGL CCESCAFIPK GHICRGSTDE
460 470 480 490 500
CDLHEYCNGS SAACQEDVYV QDGHPCGQNQ WLCISGICVD GIKQCFDIFG
510 520 530 540 550
EGTSYAPAEC FQRLNSMNDL SGNCGVTPTG FTPCTSENVR CGKLLCTYDK
560 570 580 590 600
REVISVENAS VMYSNINGKI CIGLHYEYGN EDEGMMWVKD GTVCGESKIC
610 620 630 640 650
QNKQCVDSSF LNYDCNPEKC NNQGVCNNKK HCHCNPSYLP PNCEHSAPGW
660 670 680 690 700
EGGSIDSGNF PPSEPPTGGP AFTDVGTTPL AESRYIENVY RSKPTRWPFF
710 720 730 740
LFIPFFIILC VLIATLVKVY FQRKKWRTED YTSDEQLESE SEPKD
Length:745
Mass (Da):83,150
Last modified:November 1, 1998 - v1
Checksum:iB5D8DC0168999B00
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF086808 mRNA. Translation: AAC62753.1.
RefSeqiNP_776653.1. NM_174228.1.
UniGeneiBt.139.

Genome annotation databases

GeneIDi281599.
KEGGibta:281599.

Similar proteinsi

Entry informationi

Entry nameiADAM2_BOVIN
AccessioniPrimary (citable) accession number: O77780
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 2003
Last sequence update: November 1, 1998
Last modified: October 25, 2017
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome