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Protein

Neutrophil cytosol factor 2

Gene

NCF2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).

GO - Molecular functioni

GO - Biological processi

Names & Taxonomyi

Protein namesi
Recommended name:
Neutrophil cytosol factor 2
Short name:
NCF-2
Alternative name(s):
67 kDa neutrophil oxidase factor
Neutrophil NADPH oxidase factor 2
p67-phox
Gene namesi
Name:NCF2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001063601 – 527Neutrophil cytosol factor 2Add BLAST527

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei233PhosphothreonineBy similarity1
Modified residuei324PhosphoserineBy similarity1
Modified residuei400PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO77775
PeptideAtlasiO77775
PRIDEiO77775

Interactioni

Subunit structurei

Component of an NADPH oxidase complex composed of a heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic subunits NCF1, NCF2 and NCF4. Interacts with NCF4. Interacts (via the C-terminal SH3 domain) with NCF1 (via C-terminus). Interacts with SYTL1 and RAC1. May interact with NOXO1. Interacts with S100A8 and calprotectin (S100A8/9) (By similarity).By similarity

Protein-protein interaction databases

IntActiO77775, 2 interactors
STRINGi9913.ENSBTAP00000010525

Structurei

3D structure databases

ProteinModelPortaliO77775
SMRiO77775
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati37 – 70TPR 1Add BLAST34
Repeati71 – 104TPR 2Add BLAST34
Repeati121 – 154TPR 3Add BLAST34
Domaini240 – 299SH3 1PROSITE-ProRule annotationAdd BLAST60
Domaini352 – 430PB1PROSITE-ProRule annotationAdd BLAST79
Domaini458 – 517SH3 2PROSITE-ProRule annotationAdd BLAST60

Domaini

The OPR/PB1 domain mediates the association with NCF4/p40-PHOX.By similarity

Sequence similaritiesi

Belongs to the NCF2/NOXA1 family.Curated

Keywords - Domaini

Repeat, SH3 domain, TPR repeat

Phylogenomic databases

eggNOGiKOG4225 Eukaryota
ENOG41110AD LUCA
HOGENOMiHOG000237312
HOVERGENiHBG001521
InParanoidiO77775
KOiK08010

Family and domain databases

CDDicd06406 PB1_P67, 1 hit
cd11871 SH3_p67phox_N, 1 hit
Gene3Di1.25.40.10, 1 hit
InterProiView protein in InterPro
IPR034889 P67-PHOX
IPR035546 p67phox_SH3_1
IPR000270 PB1_dom
IPR034885 PB1_P67
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
IPR013026 TPR-contain_dom
IPR011990 TPR-like_helical_dom_sf
IPR001440 TPR_1
IPR019734 TPR_repeat
PANTHERiPTHR15175:SF3 PTHR15175:SF3, 1 hit
PfamiView protein in Pfam
PF00564 PB1, 1 hit
PF00018 SH3_1, 2 hits
PF00515 TPR_1, 1 hit
PF13181 TPR_8, 1 hit
PRINTSiPR00452 SH3DOMAIN
SMARTiView protein in SMART
SM00666 PB1, 1 hit
SM00326 SH3, 2 hits
SM00028 TPR, 3 hits
SUPFAMiSSF48452 SSF48452, 1 hit
SSF50044 SSF50044, 2 hits
PROSITEiView protein in PROSITE
PS51745 PB1, 1 hit
PS50002 SH3, 2 hits
PS50005 TPR, 3 hits
PS50293 TPR_REGION, 2 hits

Sequencei

Sequence statusi: Complete.

O77775-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLAEAISLW NEGVLAADKK DWKGALDAFT GVQDPHSRIC FNVGCIYTIL
60 70 80 90 100
GNLPEAEKAF TKSINRDKHL AVSYFQRGML YYQMEKYDSA IKDLKEALTQ
110 120 130 140 150
LRGNQLIDYK ILGLQFKLFA CEVLYNIAFM YAKREEWKKA EEHLALAVSM
160 170 180 190 200
KSEPRHSKID RAMESVWKQK LYEPVVIPVG RLFRPNEKQV AQLVKKDYLG
210 220 230 240 250
KATVVASVVD QDSFSGFAPL QPQAAEPPPR PKTPEIFRAL EGEAHRVLFG
260 270 280 290 300
FVPETPEELQ VMPGNIVFVL KKGNDNWATV MFNGQKGLVP CNYLEPVELR
310 320 330 340 350
IHPQQQPQEE TSLESDIPAP PSSSAPGRPQ LSPGQKGKEE PKQEIKLSVP
360 370 380 390 400
KSYTLKVHYK YTVVMETQFR LPYSQVRDMV AKKLDLLPEH TKLSYRRQDS
410 420 430 440 450
NELVPLSEFS MKDAWAQVKN YCLTLWCENT VGDQGFPDEP EESKKSDANN
460 470 480 490 500
QTTEPELKEG SKVVALFSYE ATQPEDLEFL EGDVILVIST VNEQWLEGEC
510 520
KGKVGIFPKA FVEQHPTTDL ESTPGRV
Length:527
Mass (Da):59,663
Last modified:November 1, 1998 - v1
Checksum:i430D33FE1DF68BD1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF079303 mRNA Translation: AAC82463.1
RefSeqiNP_776545.1, NM_174120.2
UniGeneiBt.143

Genome annotation databases

GeneIDi281346
KEGGibta:281346

Similar proteinsi

Entry informationi

Entry nameiNCF2_BOVIN
AccessioniPrimary (citable) accession number: O77775
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: February 28, 2018
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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