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Protein

Neutrophil cytosol factor 2

Gene

NCF2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).

Names & Taxonomyi

Protein namesi
Recommended name:
Neutrophil cytosol factor 2
Short name:
NCF-2
Alternative name(s):
67 kDa neutrophil oxidase factor
Neutrophil NADPH oxidase factor 2
p67-phox
Gene namesi
Name:NCF2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 527527Neutrophil cytosol factor 2PRO_0000106360Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei233 – 2331PhosphothreonineBy similarity
Modified residuei324 – 3241PhosphoserineBy similarity
Modified residuei400 – 4001PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO77775.
PRIDEiO77775.

Interactioni

Subunit structurei

Component of an NADPH oxidase complex composed of a heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic subunits NCF1, NCF2 and NCF4. Interacts with NCF4. Interacts (via the C-terminal SH3 domain) with NCF1 (via C-terminus). Interacts with SYTL1 and RAC1. May interact with NOXO1. Interacts with S100A8 and calprotectin (S100A8/9) (By similarity).By similarity

Protein-protein interaction databases

IntActiO77775. 2 interactions.
STRINGi9913.ENSBTAP00000010525.

Structurei

3D structure databases

ProteinModelPortaliO77775.
SMRiO77775. Positions 1-203, 242-297, 352-429, 456-517.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati37 – 7034TPR 1Add
BLAST
Repeati71 – 10434TPR 2Add
BLAST
Repeati121 – 15434TPR 3Add
BLAST
Domaini240 – 29960SH3 1PROSITE-ProRule annotationAdd
BLAST
Domaini352 – 43079PB1PROSITE-ProRule annotationAdd
BLAST
Domaini458 – 51760SH3 2PROSITE-ProRule annotationAdd
BLAST

Domaini

The OPR/PB1 domain mediates the association with NCF4/p40-PHOX.By similarity

Sequence similaritiesi

Belongs to the NCF2/NOXA1 family.Curated
Contains 1 PB1 domain.PROSITE-ProRule annotation
Contains 2 SH3 domains.PROSITE-ProRule annotation
Contains 3 TPR repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH3 domain, TPR repeat

Phylogenomic databases

eggNOGiKOG4225. Eukaryota.
ENOG41110AD. LUCA.
HOGENOMiHOG000237312.
HOVERGENiHBG001521.
InParanoidiO77775.
KOiK08010.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR000270. PB1_dom.
IPR001452. SH3_domain.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF00564. PB1. 1 hit.
PF00018. SH3_1. 2 hits.
PF00515. TPR_1. 1 hit.
PF13181. TPR_8. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00666. PB1. 1 hit.
SM00326. SH3. 2 hits.
SM00028. TPR. 3 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.
SSF50044. SSF50044. 2 hits.
PROSITEiPS51745. PB1. 1 hit.
PS50002. SH3. 2 hits.
PS50005. TPR. 3 hits.
PS50293. TPR_REGION. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O77775-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLAEAISLW NEGVLAADKK DWKGALDAFT GVQDPHSRIC FNVGCIYTIL
60 70 80 90 100
GNLPEAEKAF TKSINRDKHL AVSYFQRGML YYQMEKYDSA IKDLKEALTQ
110 120 130 140 150
LRGNQLIDYK ILGLQFKLFA CEVLYNIAFM YAKREEWKKA EEHLALAVSM
160 170 180 190 200
KSEPRHSKID RAMESVWKQK LYEPVVIPVG RLFRPNEKQV AQLVKKDYLG
210 220 230 240 250
KATVVASVVD QDSFSGFAPL QPQAAEPPPR PKTPEIFRAL EGEAHRVLFG
260 270 280 290 300
FVPETPEELQ VMPGNIVFVL KKGNDNWATV MFNGQKGLVP CNYLEPVELR
310 320 330 340 350
IHPQQQPQEE TSLESDIPAP PSSSAPGRPQ LSPGQKGKEE PKQEIKLSVP
360 370 380 390 400
KSYTLKVHYK YTVVMETQFR LPYSQVRDMV AKKLDLLPEH TKLSYRRQDS
410 420 430 440 450
NELVPLSEFS MKDAWAQVKN YCLTLWCENT VGDQGFPDEP EESKKSDANN
460 470 480 490 500
QTTEPELKEG SKVVALFSYE ATQPEDLEFL EGDVILVIST VNEQWLEGEC
510 520
KGKVGIFPKA FVEQHPTTDL ESTPGRV
Length:527
Mass (Da):59,663
Last modified:November 1, 1998 - v1
Checksum:i430D33FE1DF68BD1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF079303 mRNA. Translation: AAC82463.1.
RefSeqiNP_776545.1. NM_174120.2.
UniGeneiBt.143.

Genome annotation databases

GeneIDi281346.
KEGGibta:281346.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF079303 mRNA. Translation: AAC82463.1.
RefSeqiNP_776545.1. NM_174120.2.
UniGeneiBt.143.

3D structure databases

ProteinModelPortaliO77775.
SMRiO77775. Positions 1-203, 242-297, 352-429, 456-517.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO77775. 2 interactions.
STRINGi9913.ENSBTAP00000010525.

Proteomic databases

PaxDbiO77775.
PRIDEiO77775.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi281346.
KEGGibta:281346.

Organism-specific databases

CTDi4688.

Phylogenomic databases

eggNOGiKOG4225. Eukaryota.
ENOG41110AD. LUCA.
HOGENOMiHOG000237312.
HOVERGENiHBG001521.
InParanoidiO77775.
KOiK08010.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR000270. PB1_dom.
IPR001452. SH3_domain.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF00564. PB1. 1 hit.
PF00018. SH3_1. 2 hits.
PF00515. TPR_1. 1 hit.
PF13181. TPR_8. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00666. PB1. 1 hit.
SM00326. SH3. 2 hits.
SM00028. TPR. 3 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.
SSF50044. SSF50044. 2 hits.
PROSITEiPS51745. PB1. 1 hit.
PS50002. SH3. 2 hits.
PS50005. TPR. 3 hits.
PS50293. TPR_REGION. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and expression of bovine p47-phox and p67-phox: comparison with the human and murine homologs."
    Bunger P.L., Swain S.D., Clements M.K., Siemsen D.W., Davis A.R., Gauss K.A., Quinn M.T.
    J. Leukoc. Biol. 67:63-72(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiNCF2_BOVIN
AccessioniPrimary (citable) accession number: O77775
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: June 8, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.