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Protein

Neutrophil cytosol factor 1

Gene

NCF1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Lipid-binding

Enzyme and pathway databases

ReactomeiR-BTA-1222556. ROS, RNS production in response to bacteria.
R-BTA-1236973. Cross-presentation of particulate exogenous antigens (phagosomes).
R-BTA-4420097. VEGFA-VEGFR2 Pathway.
R-BTA-5668599. RHO GTPases Activate NADPH Oxidases.

Names & Taxonomyi

Protein namesi
Recommended name:
Neutrophil cytosol factor 1
Short name:
NCF-1
Alternative name(s):
47 kDa neutrophil oxidase factor
NCF-47K
Neutrophil NADPH oxidase factor 1
p47-phox
Gene namesi
Name:NCF1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 25

Subcellular locationi

  • Cytoplasmcytosol 1 Publication
  • Membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000967611 – 392Neutrophil cytosol factor 1Add BLAST392

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei304PhosphoserineBy similarity1
Modified residuei305PhosphoserineBy similarity1
Modified residuei321PhosphoserineBy similarity1
Modified residuei329PhosphoserineBy similarity1
Modified residuei348PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by PRKCD; phosphorylation induces activation of NCF1 and NADPH oxidase activity.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO77774.
PRIDEiO77774.

Expressioni

Gene expression databases

BgeeiENSBTAG00000003305.

Interactioni

Subunit structurei

Component of an NADPH oxidase complex composed of a heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic subunits NCF1, NCF2 and NCF4. Interacts (via C-terminus) with NCF2 (via the C-terminal SH3 domain). Interacts with NCF4. Interacts with CYBB. Interacts (via the second SH3 domain) with CYBA. Interacts with NOXA1. Interacts with ADAM15. Interacts with TRAF4. Interacts with FASLG (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000004279.

Structurei

3D structure databases

ProteinModelPortaliO77774.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 125PXPROSITE-ProRule annotationAdd BLAST122
Domaini156 – 215SH3 1PROSITE-ProRule annotationAdd BLAST60
Domaini226 – 285SH3 2PROSITE-ProRule annotationAdd BLAST60

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi211 – 254Asp/Glu-rich (highly acidic)Add BLAST44
Compositional biasi292 – 392Arg/Lys-rich (highly basic)Add BLAST101

Domaini

The PX domain mediates interaction with phosphatidylinositol 3,4-bisphosphate and other anionic phospholipids. In the autoinhibited, unphosphorylated state an intramolecular interaction with the C-terminal SH3 domain precludes phospholipid binding and interaction with CYBA. Phosphorylation disrupts the autoinhibited state (By similarity).By similarity

Sequence similaritiesi

Contains 1 PX (phox homology) domain.PROSITE-ProRule annotation
Contains 2 SH3 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH3 domain

Phylogenomic databases

eggNOGiENOG410IRR3. Eukaryota.
ENOG410YYZE. LUCA.
GeneTreeiENSGT00530000063010.
HOGENOMiHOG000232124.
HOVERGENiHBG002055.
InParanoidiO77774.
KOiK08011.
OMAiNGWWFCQ.
OrthoDBiEOG091G0CBF.
TreeFamiTF329347.

Family and domain databases

Gene3Di3.30.1520.10. 1 hit.
InterProiIPR015039. NADPH_oxidase_p47Phox_C.
IPR032136. NECFESHC.
IPR001655. P47PHOX.
IPR001683. Phox.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF16621. NECFESHC. 1 hit.
PF08944. p47_phox_C. 1 hit.
PF00787. PX. 1 hit.
PF00018. SH3_1. 2 hits.
[Graphical view]
PRINTSiPR00498. P47PHOX.
PR00452. SH3DOMAIN.
SMARTiSM00312. PX. 1 hit.
SM00326. SH3. 2 hits.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 2 hits.
SSF64268. SSF64268. 1 hit.
PROSITEiPS50195. PX. 1 hit.
PS50002. SH3. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O77774-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDHFIRHIA LLGFEKRFVP SQHYVYMFLV KWQDLSEKVV YRRFTEIYEF
60 70 80 90 100
HKILKEMFPI EAGDINPENR IIPHLPAPRW YDGQRVAESR QGTLTEYCST
110 120 130 140 150
LMSLPVKISR CPHLLNFFKV RPDDLKLPTD SQVKKPETYL MPKDGKNNAA
160 170 180 190 200
DITGPIILQT YRAIADYEKG SSSQMALATG DVVDVVEKNE SGWWFCQMKT
210 220 230 240 250
KRGWVPASYL EPLDSPDEAE DPEPNYAGEP YITIKAYTAV LEDEISLEEG
260 270 280 290 300
EAIEVIHKLL DGWWVIRKED VTGYFPSMYL QKAGQDVAQA KSQIKSRGAP
310 320 330 340 350
PRRSSIRNAH SIHQRSRKRL SQDTYRRNSV RFMQQRRHQR PEPQRSRSAL
360 370 380 390
REQQQPKTER PKPQPAVPPR PSADLILHRC SESTKRKLAS AV
Length:392
Mass (Da):45,346
Last modified:November 1, 1998 - v1
Checksum:i79199BD86AE80DB7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF079302 mRNA. Translation: AAC82462.1.
BT020852 mRNA. Translation: AAX08869.1.
BT021181 mRNA. Translation: AAX31363.1.
BC103145 mRNA. Translation: AAI03146.1.
RefSeqiNP_776544.1. NM_174119.4.
UniGeneiBt.142.

Genome annotation databases

EnsembliENSBTAT00000004279; ENSBTAP00000004279; ENSBTAG00000003305.
GeneIDi281345.
KEGGibta:281345.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF079302 mRNA. Translation: AAC82462.1.
BT020852 mRNA. Translation: AAX08869.1.
BT021181 mRNA. Translation: AAX31363.1.
BC103145 mRNA. Translation: AAI03146.1.
RefSeqiNP_776544.1. NM_174119.4.
UniGeneiBt.142.

3D structure databases

ProteinModelPortaliO77774.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000004279.

Proteomic databases

PaxDbiO77774.
PRIDEiO77774.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000004279; ENSBTAP00000004279; ENSBTAG00000003305.
GeneIDi281345.
KEGGibta:281345.

Organism-specific databases

CTDi653361.

Phylogenomic databases

eggNOGiENOG410IRR3. Eukaryota.
ENOG410YYZE. LUCA.
GeneTreeiENSGT00530000063010.
HOGENOMiHOG000232124.
HOVERGENiHBG002055.
InParanoidiO77774.
KOiK08011.
OMAiNGWWFCQ.
OrthoDBiEOG091G0CBF.
TreeFamiTF329347.

Enzyme and pathway databases

ReactomeiR-BTA-1222556. ROS, RNS production in response to bacteria.
R-BTA-1236973. Cross-presentation of particulate exogenous antigens (phagosomes).
R-BTA-4420097. VEGFA-VEGFR2 Pathway.
R-BTA-5668599. RHO GTPases Activate NADPH Oxidases.

Gene expression databases

BgeeiENSBTAG00000003305.

Family and domain databases

Gene3Di3.30.1520.10. 1 hit.
InterProiIPR015039. NADPH_oxidase_p47Phox_C.
IPR032136. NECFESHC.
IPR001655. P47PHOX.
IPR001683. Phox.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF16621. NECFESHC. 1 hit.
PF08944. p47_phox_C. 1 hit.
PF00787. PX. 1 hit.
PF00018. SH3_1. 2 hits.
[Graphical view]
PRINTSiPR00498. P47PHOX.
PR00452. SH3DOMAIN.
SMARTiSM00312. PX. 1 hit.
SM00326. SH3. 2 hits.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 2 hits.
SSF64268. SSF64268. 1 hit.
PROSITEiPS50195. PX. 1 hit.
PS50002. SH3. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNCF1_BOVIN
AccessioniPrimary (citable) accession number: O77774
Secondary accession number(s): Q5E9R8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: October 5, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.