Reviewed, UniProtKB/Swiss-Prot O77761 (SOAT1_MACFA)

Names and origin

Protein names

Recommended name:
    Sterol O-acyltransferase 1
    EC=2.3.1.26
Alternative name(s):
    Cholesterol acyltransferase 1
    Acyl coenzyme A:cholesterol acyltransferase 1
      Short name=ACAT-1

Gene names

Name:	SOAT1
Synonyms:	ACAT, ACAT1

Organism

Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	550 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Plays a role in lipoprotein assembly and dietary cholesterol absorption. In addition to its acyltransferase activity, it may act as a ligase By similarity.
Catalytic activity	Acyl-CoA + cholesterol = CoA + cholesterol ester.
Subunit structure	May form homo- or heterodimers By similarity.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein.
Tissue specificity	Expressed in most tissues, but most strongly in the adrenal gland. Expressed more strongly in liver Kupffer cells than in hepatocytes.
Sequence similarities	Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.

Ontologies

Keywords
Biological process	Cholesterol metabolism Lipid metabolism Steroid metabolism
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological process	cholesterol metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cholesterol O-acyltransferase activity Inferred from electronic annotation. Source: EC ergosterol O-acyltransferase activity Inferred from electronic annotation. Source: EC lanosterol O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 550	550	Sterol O-acyltransferase 1		PRO_0000207641
Regions
	Transmembrane	142 – 162	21	Potential
	Transmembrane	321 – 341	21	Potential
	Transmembrane	362 – 382	21	Potential
	Transmembrane	470 – 490	21	Potential
	Transmembrane	498 – 518	21	Potential
Sites
	Active site	460	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	O77761-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: AA655206D58C291D	FASTA	550	64,700
	10 20 30 40 50 60 MVGEEKMSLR NRLSKSRENP EEDEDQRKPA KESLEAPSNG RIDIKQLIAK KIKLTAEAEE 70 80 90 100 110 120 LKPFFMKEVG SHFDDFVTNL IEKSASLDNG GCALTTFSIL EGEKNNHRAK DLRAPPEQGK 130 140 150 160 170 180 IFIARRSLLD ELLEVDHIRT IYHMFIALLI LFILSTLVVD YIDEGRLVLE FSLLSYAFGK 190 200 210 220 230 240 FPTVVWTWWI MFLSTFSVPY FLFQRWATGY SKSSHPLINS LFHGFLFMVF QIGILGFGPT 250 260 270 280 290 300 YVVLAYTLPP ASRFIIIFEQ IRFVMKAHSF VRENVPRVLN SAKEKSSTVP IPTVNQYLYF 310 320 330 340 350 360 LFAPTLIYRD SYPRNPTVRW GYVAMQFAQV FGCFFYVYYI FERLCAPLFR NIKQEPFSAR 370 380 390 400 410 420 VLVLCVFNSI LPGVLILFLT FFAFLHCWLN AFAEMLRFGD RMFYKDWWNS TSYSNYYRTW 430 440 450 460 470 480 NVVVHDWLYY YAYKDFLWFF SKRFKSAAML AAFAVSAVVH EYALAVCLSF FYPVLFVLFM 490 500 510 520 530 540 FFGMAFNFIV NDSRKKPIWN VMMWTSLFLG NGVLLCFYSQ EWYARQHCPL KNPTFLDYVR 550 PRSWTCRYVF

References

[1]

"Identification of a form of acyl-CoA:cholesterol acyltransferase specific to liver and intestine in nonhuman primates." Anderson R.A., Joyce C., Davis M., Reagan J.W., Clark M., Shelness G.S., Rudel L.L. J. Biol. Chem. 273:26747-26754(1998) [PubMed: 9756918] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Adrenal gland.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF053337 mRNA. Translation: AAC62931.1.
3D structure databases
ModBase	Search...
Phylogenomic databases
HOVERGEN	O77761.
Enzyme and pathway databases
BRENDA	2.3.1.26. 3438.
Family and domain databases
InterPro	IPR004299. MBOAT_fam. [Graphical view]
Pfam	PF03062. MBOAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SOAT1_MACFA

Accession

Primary (citable) accession number: O77761

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 18, 2001
Last sequence update:	November 1, 1998
Last modified:	October 13, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)