Reviewed, UniProtKB/Swiss-Prot O77759 (SOAT2_CERAE)

Names and origin

Protein names

Recommended name:
    Sterol O-acyltransferase 2
    EC=2.3.1.26
Alternative name(s):
    Cholesterol acyltransferase 2
    Acyl coenzyme A:cholesterol acyltransferase 2
      Short name=ACAT-2

Gene names

Name:	SOAT2
Synonyms:	ACAT2

Organism

Cercopithecus aethiops (Green monkey) (Grivet)

Taxonomic identifier

9534 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Chlorocebus

Protein attributes

Sequence length	526 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Plays a role in lipoprotein assembly and dietary cholesterol absorption. In addition to its acyltransferase activity, it may act as a ligase. May provide cholesteryl esters for lipoprotein secretion from hepatocytes and intestinal mucosa.
Catalytic activity	Acyl-CoA + cholesterol = CoA + cholesterol ester.
Subunit structure	May form homo- or heterodimers By similarity.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein.
Tissue specificity	Liver and intestine. Expression is six-fold greater in hepatocytes than in kupffer cells.
Sequence similarities	Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.

Ontologies

Keywords
Biological process	Cholesterol metabolism Lipid metabolism Steroid metabolism
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological process	cholesterol metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cholesterol O-acyltransferase activity Inferred from electronic annotation. Source: EC ergosterol O-acyltransferase activity Inferred from electronic annotation. Source: EC lanosterol O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 526	526	Sterol O-acyltransferase 2		PRO_0000207644
Regions
	Transmembrane	124 – 144	21	Potential
	Transmembrane	155 – 175	21	Potential
	Transmembrane	200 – 220	21	Potential
	Transmembrane	308 – 328	21	Potential
	Transmembrane	340 – 360	21	Potential
	Transmembrane	416 – 436	21	Potential
	Transmembrane	441 – 461	21	Potential
	Transmembrane	476 – 496	21	Potential
Sites
	Active site	438	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	O77759-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: FA5E29F48386DB43	FASTA	526	60,412
	10 20 30 40 50 60 MEPGGARLRL QRTEGPGGER EHQPCRDGNT ETHRAPDLVK WTRHMEAVKA QLLEQAQGQL 70 80 90 100 110 120 RELLDRAMWE AIQSYPSQDK PPPLPPPDSL SRTQEPSLGK QKVFIIRKSL LDELMEVQHF 130 140 150 160 170 180 RTIYHMFIAG LCVFIISTLA IDFIDEGRLL LEFDLLIFSF GQLPLALVTW VPMFLSTLLA 190 200 210 220 230 240 PYQALRLWAR PGARGTWTLG AGLGCALLAA HALVLCALPV HVAVEHQLPP ASRCVLVFEQ 250 260 270 280 290 300 VRFLMKSYSF LREAVPGTLR ARRGEGIQAP SFSSYLYFLF CPTLIYRETY PRTPYIRWNY 310 320 330 340 350 360 VAKNFAQALG CVLYACFILG RLCVPVFANM SREPFSTRAL VLSILHATLP GIFMLLLIFF 370 380 390 400 410 420 AFLHCWLNAF AEMLRFGDRM FYRDWWNSTS FSNYYRTWNV VVHDWLYSYV YQDGLWLLGA 430 440 450 460 470 480 QARGVAMLGV FLVSAVAHEY IFCFVLGFFY PVMLILFLVI GGMLNFMMHD QHTGPAWNVL 490 500 510 520 MWTMLFLGQG IQVSLYCQEW YARRHCPLPQ TTFWGLVTPR SWSCHT

References

[1]

"Identification of a form of acyl-CoA:cholesterol acyltransferase specific to liver and intestine in nonhuman primates." Anderson R.A., Joyce C., Davis M., Reagan J.W., Clark M., Shelness G.S., Rudel L.L. J. Biol. Chem. 273:26747-26754(1998) [PubMed: 9756918] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF053234 mRNA. Translation: AAC62929.1.
3D structure databases
ModBase	Search...
Phylogenomic databases
HOVERGEN	O77759.
Enzyme and pathway databases
BRENDA	2.3.1.26. 2052.
Family and domain databases
InterPro	IPR004299. MBOAT_fam. [Graphical view]
Pfam	PF03062. MBOAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SOAT2_CERAE

Accession

Primary (citable) accession number: O77759

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 18, 2001
Last sequence update:	November 1, 1998
Last modified:	October 13, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)