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Protein

Aquaporin-4

Gene

AQP4

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiR-BTA-432040. Vasopressin regulates renal water homeostasis via Aquaporins.
R-BTA-432047. Passive transport by Aquaporins.

Names & Taxonomyi

Protein namesi
Recommended name:
Aquaporin-4
Short name:
AQP-4
Alternative name(s):
Mercurial-insensitive water channel
Short name:
MIWC
WCH4
Gene namesi
Name:AQP4
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 24

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 36CytoplasmicSequence analysisAdd BLAST36
Transmembranei37 – 57HelicalSequence analysisAdd BLAST21
Topological domaini58 – 64ExtracellularSequence analysis7
Transmembranei65 – 85HelicalSequence analysisAdd BLAST21
Topological domaini86 – 115CytoplasmicSequence analysisAdd BLAST30
Transmembranei116 – 136HelicalSequence analysisAdd BLAST21
Topological domaini137 – 155ExtracellularSequence analysisAdd BLAST19
Transmembranei156 – 176HelicalSequence analysisAdd BLAST21
Topological domaini177 – 184CytoplasmicSequence analysis8
Transmembranei185 – 205HelicalSequence analysisAdd BLAST21
Topological domaini206 – 231ExtracellularSequence analysisAdd BLAST26
Transmembranei232 – 252HelicalSequence analysisAdd BLAST21
Topological domaini253 – 323CytoplasmicSequence analysisAdd BLAST71

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000639461 – 323Aquaporin-4Add BLAST323

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei111Phosphoserine; by PKGBy similarity1
Glycosylationi153N-linked (GlcNAc...)Sequence analysis1
Modified residuei180Phosphoserine; by PKCBy similarity1
Glycosylationi206N-linked (GlcNAc...)Sequence analysis1
Modified residuei276PhosphoserineBy similarity1
Modified residuei285PhosphoserineBy similarity1
Modified residuei289PhosphothreonineBy similarity1
Modified residuei321PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation by PKC at Ser-180 reduces conductance by 50%. Phosphorylation by PKG at Ser-111 in response to glutamats increases conductance by 40% (By similarity).By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiO77750.
PRIDEiO77750.

Expressioni

Gene expression databases

BgeeiENSBTAG00000019037.

Interactioni

Subunit structurei

Homotetramer. Part of a complex containing MLC1, TRPV4, HEPACAM and ATP1B1.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000025341.

Structurei

3D structure databases

ProteinModelPortaliO77750.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi97 – 99NPA 13
Motifi213 – 215NPA 23

Domaini

Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0223. Eukaryota.
COG0580. LUCA.
GeneTreeiENSGT00760000119223.
HOGENOMiHOG000288286.
HOVERGENiHBG000312.
InParanoidiO77750.
KOiK09866.
OMAiCRRESIM.
OrthoDBiEOG091G166T.
TreeFamiTF312940.

Family and domain databases

CDDicd00333. MIP. 1 hit.
Gene3Di1.20.1080.10. 1 hit.
InterProiIPR023271. Aquaporin-like.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view]
PANTHERiPTHR19139. PTHR19139. 1 hit.
PfamiPF00230. MIP. 1 hit.
[Graphical view]
PRINTSiPR00783. MINTRINSICP.
SUPFAMiSSF81338. SSF81338. 1 hit.
TIGRFAMsiTIGR00861. MIP. 1 hit.
PROSITEiPS00221. MIP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 2 (identifier: O77750-1) [UniParc]FASTAAdd to basket
Also known as: B

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSDRPAARRW GKCGPLCTRE SIMVAFKGVW TQTFWKAVTA EFLAMLIFVL
60 70 80 90 100
LSLGSTINWG GAEKPLPVDM VLISLCFGLS IATMVQCFGH ISGGHINPAV
110 120 130 140 150
TVAMVCTRRI SIAKSVFYIA AQCLGAIIGA GILYLVTPPS VVGGLGVTTV
160 170 180 190 200
HGNLSAGHGL LVELIITFQL VFTIFASCDS KRTDVTGSIA LAIGISVAIG
210 220 230 240 250
HLFAINYTGA SMNPARSFGP AVIMGNWENH WIYWVGPIIG AVLAGGLYEY
260 270 280 290 300
VFCPDVELKR RFKEAFSKAA QQTKGSYMEV EDNRSQVETD DLILKPGVVH
310 320
VIDIDRGEEK KGKDPSGEVL SSV
Length:323
Mass (Da):34,672
Last modified:October 17, 2006 - v3
Checksum:iCFFF473A47D94CC3
GO
Isoform 1 (identifier: O77750-2) [UniParc]FASTAAdd to basket
Also known as: A

The sequence of this isoform differs from the canonical sequence as follows:
     1-22: Missing.

Show »
Length:301
Mass (Da):32,199
Checksum:i20E71E5FF11F77A7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti8R → T (PubMed:10673041).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0032311 – 22Missing in isoform 1. 2 PublicationsAdd BLAST22

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB015947 mRNA. Translation: BAA36505.2.
AB012950 mRNA. Translation: BAA33583.1.
AB028642 mRNA. Translation: BAA89291.1.
BC118415 mRNA. Translation: AAI18416.1.
RefSeqiNP_001304721.1. NM_001317792.1.
NP_001304723.1. NM_001317794.1. [O77750-2]
NP_851346.1. NM_181003.3. [O77750-1]
XP_005224121.1. XM_005224064.3. [O77750-2]
UniGeneiBt.5456.

Genome annotation databases

EnsembliENSBTAT00000025341; ENSBTAP00000025341; ENSBTAG00000019037. [O77750-1]
GeneIDi281008.
KEGGibta:281008.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB015947 mRNA. Translation: BAA36505.2.
AB012950 mRNA. Translation: BAA33583.1.
AB028642 mRNA. Translation: BAA89291.1.
BC118415 mRNA. Translation: AAI18416.1.
RefSeqiNP_001304721.1. NM_001317792.1.
NP_001304723.1. NM_001317794.1. [O77750-2]
NP_851346.1. NM_181003.3. [O77750-1]
XP_005224121.1. XM_005224064.3. [O77750-2]
UniGeneiBt.5456.

3D structure databases

ProteinModelPortaliO77750.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000025341.

Proteomic databases

PaxDbiO77750.
PRIDEiO77750.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000025341; ENSBTAP00000025341; ENSBTAG00000019037. [O77750-1]
GeneIDi281008.
KEGGibta:281008.

Organism-specific databases

CTDi361.

Phylogenomic databases

eggNOGiKOG0223. Eukaryota.
COG0580. LUCA.
GeneTreeiENSGT00760000119223.
HOGENOMiHOG000288286.
HOVERGENiHBG000312.
InParanoidiO77750.
KOiK09866.
OMAiCRRESIM.
OrthoDBiEOG091G166T.
TreeFamiTF312940.

Enzyme and pathway databases

ReactomeiR-BTA-432040. Vasopressin regulates renal water homeostasis via Aquaporins.
R-BTA-432047. Passive transport by Aquaporins.

Gene expression databases

BgeeiENSBTAG00000019037.

Family and domain databases

CDDicd00333. MIP. 1 hit.
Gene3Di1.20.1080.10. 1 hit.
InterProiIPR023271. Aquaporin-like.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view]
PANTHERiPTHR19139. PTHR19139. 1 hit.
PfamiPF00230. MIP. 1 hit.
[Graphical view]
PRINTSiPR00783. MINTRINSICP.
SUPFAMiSSF81338. SSF81338. 1 hit.
TIGRFAMsiTIGR00861. MIP. 1 hit.
PROSITEiPS00221. MIP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAQP4_BOVIN
AccessioniPrimary (citable) accession number: O77750
Secondary accession number(s): Q17QE1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: October 17, 2006
Last modified: October 5, 2016
This is version 122 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.