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Protein

Aquaporin-4

Gene

AQP4

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiR-BTA-432040. Vasopressin regulates renal water homeostasis via Aquaporins.
R-BTA-432047. Passive transport by Aquaporins.

Names & Taxonomyi

Protein namesi
Recommended name:
Aquaporin-4
Short name:
AQP-4
Alternative name(s):
Mercurial-insensitive water channel
Short name:
MIWC
WCH4
Gene namesi
Name:AQP4
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 24

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3636CytoplasmicSequence analysisAdd
BLAST
Transmembranei37 – 5721HelicalSequence analysisAdd
BLAST
Topological domaini58 – 647ExtracellularSequence analysis
Transmembranei65 – 8521HelicalSequence analysisAdd
BLAST
Topological domaini86 – 11530CytoplasmicSequence analysisAdd
BLAST
Transmembranei116 – 13621HelicalSequence analysisAdd
BLAST
Topological domaini137 – 15519ExtracellularSequence analysisAdd
BLAST
Transmembranei156 – 17621HelicalSequence analysisAdd
BLAST
Topological domaini177 – 1848CytoplasmicSequence analysis
Transmembranei185 – 20521HelicalSequence analysisAdd
BLAST
Topological domaini206 – 23126ExtracellularSequence analysisAdd
BLAST
Transmembranei232 – 25221HelicalSequence analysisAdd
BLAST
Topological domaini253 – 32371CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 323323Aquaporin-4PRO_0000063946Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei111 – 1111Phosphoserine; by PKGBy similarity
Glycosylationi153 – 1531N-linked (GlcNAc...)Sequence analysis
Modified residuei180 – 1801Phosphoserine; by PKCBy similarity
Glycosylationi206 – 2061N-linked (GlcNAc...)Sequence analysis
Modified residuei276 – 2761PhosphoserineBy similarity
Modified residuei285 – 2851PhosphoserineBy similarity
Modified residuei289 – 2891PhosphothreonineBy similarity
Modified residuei321 – 3211PhosphoserineBy similarity

Post-translational modificationi

Phosphorylation by PKC at Ser-180 reduces conductance by 50%. Phosphorylation by PKG at Ser-111 in response to glutamats increases conductance by 40% (By similarity).By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiO77750.
PRIDEiO77750.

Interactioni

Subunit structurei

Homotetramer. Part of a complex containing MLC1, TRPV4, HEPACAM and ATP1B1.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000025341.

Structurei

3D structure databases

ProteinModelPortaliO77750.
SMRiO77750. Positions 31-254.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi97 – 993NPA 1
Motifi213 – 2153NPA 2

Domaini

Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0223. Eukaryota.
COG0580. LUCA.
GeneTreeiENSGT00760000119223.
HOGENOMiHOG000288286.
HOVERGENiHBG000312.
InParanoidiO77750.
KOiK09866.
OMAiCRRESIM.
OrthoDBiEOG7N8ZWD.
TreeFamiTF312940.

Family and domain databases

Gene3Di1.20.1080.10. 1 hit.
InterProiIPR023271. Aquaporin-like.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view]
PANTHERiPTHR19139. PTHR19139. 1 hit.
PfamiPF00230. MIP. 1 hit.
[Graphical view]
PRINTSiPR00783. MINTRINSICP.
SUPFAMiSSF81338. SSF81338. 1 hit.
TIGRFAMsiTIGR00861. MIP. 1 hit.
PROSITEiPS00221. MIP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 2 (identifier: O77750-1) [UniParc]FASTAAdd to basket

Also known as: B

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSDRPAARRW GKCGPLCTRE SIMVAFKGVW TQTFWKAVTA EFLAMLIFVL
60 70 80 90 100
LSLGSTINWG GAEKPLPVDM VLISLCFGLS IATMVQCFGH ISGGHINPAV
110 120 130 140 150
TVAMVCTRRI SIAKSVFYIA AQCLGAIIGA GILYLVTPPS VVGGLGVTTV
160 170 180 190 200
HGNLSAGHGL LVELIITFQL VFTIFASCDS KRTDVTGSIA LAIGISVAIG
210 220 230 240 250
HLFAINYTGA SMNPARSFGP AVIMGNWENH WIYWVGPIIG AVLAGGLYEY
260 270 280 290 300
VFCPDVELKR RFKEAFSKAA QQTKGSYMEV EDNRSQVETD DLILKPGVVH
310 320
VIDIDRGEEK KGKDPSGEVL SSV
Length:323
Mass (Da):34,672
Last modified:October 17, 2006 - v3
Checksum:iCFFF473A47D94CC3
GO
Isoform 1 (identifier: O77750-2) [UniParc]FASTAAdd to basket

Also known as: A

The sequence of this isoform differs from the canonical sequence as follows:
     1-22: Missing.

Show »
Length:301
Mass (Da):32,199
Checksum:i20E71E5FF11F77A7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti8 – 81R → T (PubMed:10673041).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2222Missing in isoform 1. 2 PublicationsVSP_003231Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB015947 mRNA. Translation: BAA36505.2.
AB012950 mRNA. Translation: BAA33583.1.
AB028642 mRNA. Translation: BAA89291.1.
BC118415 mRNA. Translation: AAI18416.1.
RefSeqiNP_001304721.1. NM_001317792.1.
NP_001304723.1. NM_001317794.1. [O77750-2]
NP_851346.1. NM_181003.3. [O77750-1]
XP_005224121.1. XM_005224064.3. [O77750-2]
UniGeneiBt.5456.

Genome annotation databases

EnsembliENSBTAT00000025341; ENSBTAP00000025341; ENSBTAG00000019037. [O77750-1]
GeneIDi281008.
KEGGibta:281008.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB015947 mRNA. Translation: BAA36505.2.
AB012950 mRNA. Translation: BAA33583.1.
AB028642 mRNA. Translation: BAA89291.1.
BC118415 mRNA. Translation: AAI18416.1.
RefSeqiNP_001304721.1. NM_001317792.1.
NP_001304723.1. NM_001317794.1. [O77750-2]
NP_851346.1. NM_181003.3. [O77750-1]
XP_005224121.1. XM_005224064.3. [O77750-2]
UniGeneiBt.5456.

3D structure databases

ProteinModelPortaliO77750.
SMRiO77750. Positions 31-254.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000025341.

Proteomic databases

PaxDbiO77750.
PRIDEiO77750.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000025341; ENSBTAP00000025341; ENSBTAG00000019037. [O77750-1]
GeneIDi281008.
KEGGibta:281008.

Organism-specific databases

CTDi361.

Phylogenomic databases

eggNOGiKOG0223. Eukaryota.
COG0580. LUCA.
GeneTreeiENSGT00760000119223.
HOGENOMiHOG000288286.
HOVERGENiHBG000312.
InParanoidiO77750.
KOiK09866.
OMAiCRRESIM.
OrthoDBiEOG7N8ZWD.
TreeFamiTF312940.

Enzyme and pathway databases

ReactomeiR-BTA-432040. Vasopressin regulates renal water homeostasis via Aquaporins.
R-BTA-432047. Passive transport by Aquaporins.

Family and domain databases

Gene3Di1.20.1080.10. 1 hit.
InterProiIPR023271. Aquaporin-like.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view]
PANTHERiPTHR19139. PTHR19139. 1 hit.
PfamiPF00230. MIP. 1 hit.
[Graphical view]
PRINTSiPR00783. MINTRINSICP.
SUPFAMiSSF81338. SSF81338. 1 hit.
TIGRFAMsiTIGR00861. MIP. 1 hit.
PROSITEiPS00221. MIP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of two bovine aquaporin-4 cDNA isoforms and their expression in brain endothelial cells."
    Sobue K., Yamamoto N., Yoneda K., Fujita K., Miura Y., Asai K., Tsuda T., Katsuya H., Kato T.
    Biochim. Biophys. Acta 1489:393-398(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
    Strain: Holstein.
    Tissue: Brain.
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: Hereford.
    Tissue: Fetal pons.

Entry informationi

Entry nameiAQP4_BOVIN
AccessioniPrimary (citable) accession number: O77750
Secondary accession number(s): Q17QE1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: October 17, 2006
Last modified: July 6, 2016
This is version 120 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.