Reviewed, UniProtKB/Swiss-Prot O77695 (BGLR_CERAE)

Names and origin

Protein names

Recommended name:
    Beta-glucuronidase
    EC=3.2.1.31

Gene names

Name:

GUSB

Organism

Cercopithecus aethiops (Green monkey) (Grivet)

Taxonomic identifier

9534 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Chlorocebus

Protein attributes

Sequence length	648 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Plays an important role in the degradation of dermatan and keratan sulfates By similarity.
Catalytic activity	A beta-D-glucuronoside + H2O = D-glucuronate + an alcohol.
Enzyme regulation	Inhibited by L-aspartic acid By similarity.
Subunit structure	Homotetramer By similarity.
Subcellular location	Lysosome.
Sequence similarities	Belongs to the glycosyl hydrolase 2 family.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-glucuronidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	‹1 – 19	›19	By similarity
	Chain	20 – 648	629	Beta-glucuronidase		PRO_0000012159
Sites
	Active site	448	1	Proton donor
Amino acid modifications
	Glycosylation	170	1	N-linked (GlcNAc...) Potential
	Glycosylation	269	1	N-linked (GlcNAc...) Potential
	Glycosylation	417	1	N-linked (GlcNAc...) Potential
	Glycosylation	628	1	N-linked (GlcNAc...) Potential
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	O77695-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: D4BB309C0D9F38D4	FASTA	648	74,632
	10 20 30 40 50 60 GLAMAWAVLG PLLWGCALAL QGGMLYPRES QSRERKELDG LWSFRADFSD NRRRGFEEQW 70 80 90 100 110 120 YRRPLRESGP TLDMPVPSSF NDISQDWRLR HFVGWVWYER EVILPERWTQ DLSTRVVLRI 130 140 150 160 170 180 GSAHAYAIVW VNGVHTLEHE GGYLPFEADI SNLVQVGPLS SHVRITIAIN NTLTSTTLPP 190 200 210 220 230 240 GTIQYLTDIS KYPKGYFIQN TYFDFFNYAG LQRSVLLYTT PTAYIDDITV TTGVEHDTGL 250 260 270 280 290 300 VNYQISVKGS NLFELEVRLL DAENKLVANG TGIQGQLKVP GARLWWPYLM HERPAYLYSL 310 320 330 340 350 360 EVRLTAQTSL GPVSDFYTLP VGIRTVAVTE SQFLINGKPF YFHGVNKHED ADIRGKGFDW 370 380 390 400 410 420 PLLVKDFNLL RWLGANAFRT SHYPYAEEVL QMCDRYGIVV IDECPGVGLA LPQFFNNVSL 430 440 450 460 470 480 QNHMRVMEEV VRRDKNHPAV VMWSVANEPA SHLESAGYYL KMVITHTKAL DPSRPVTFVT 490 500 510 520 530 540 NSNYAADKGA PYVDVICLNS YYSWYHDYGH LELIQRQLTT QFENWYKTYQ KPIIQSEYGA 550 560 570 580 590 600 ETIVGFHQDP PLMFTEEYQK SLLEQYHVVL DQKRRKYVVG ELIWNFADFM TEQSPTRVLG 610 620 630 640 NKKGVFTRQR QPKSAAFLLR ERYWKIANET RYPHSIAKSQ CLENSPFT

References

[1]	"Partial cDNA sequence of Cercopithecus aethiops (COS7 cell) beta-glucuronidase." Vervoort R. Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF084552 mRNA. Translation: AAC34593.1.
3D structure databases
SMR	O77695. Positions 19-629.
ModBase	Search...
Protein family/group databases
CAZy	GH2. Glycoside Hydrolase Family 2.
Phylogenomic databases
HOVERGEN	O77695.
Enzyme and pathway databases
BRENDA	3.2.1.31. 2052.
Family and domain databases
InterPro	IPR008979. Galactose-bd-like. IPR006101. Glyco_hydro_2. IPR013812. Glyco_hydro_2/20_Ig-like. IPR006104. Glyco_hydro_2_carb-bd. IPR006102. Glyco_hydro_2_Ig-like. IPR006103. Glyco_hydro_2_TIM. IPR017853. Glyco_hydro_catalytic_core. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:2.60.40.320. Glyco_hydro_2/20_Ig-like. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00703. Glyco_hydro_2. 1 hit. PF02836. Glyco_hydro_2_C. 1 hit. PF02837. Glyco_hydro_2_N. 1 hit. [Graphical view]
PRINTS	PR00132. GLHYDRLASE2.
PROSITE	PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit. PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGLR_CERAE

Accession

Primary (citable) accession number: O77695

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	November 1, 1998
Last modified:	November 24, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)