Reviewed, UniProtKB/Swiss-Prot O77676 (KGP1_RABIT)

Names and origin

Protein names

Recommended name:
    cGMP-dependent protein kinase 1
      Short name=cGK 1
      Short name=cGK1
    EC=2.7.11.12

Gene names

Name:

PRKG1

Organism

Oryctolagus cuniculus (Rabbit)

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	671 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Phosphorylates PPP1R12A By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Binding of cGMP results in enzyme activation By similarity.
Subunit structure	Isoform alpha: parallel homodimer or heterodimer and also heterotetramer. Interacts directly with PPP1R12A. Non-covalent dimer of dimer of PRKG1-PRKG1 and PPP1R12A-PPP1R12A. This interaction targets PRKG1 to stress fibers to mediate smooth muscle cell relaxation and vasodilation in responses to rises in cGMP By similarity. Isoform beta: antiparallel homodimer. Part of cGMP kinase signaling complex at least composed of ACTA2/alpha-actin, CNN1/calponin H1, PLN/phospholamban, PRKG1 and ITPR1. Interacts with MRVI1 By similarity.
Domain	Heterotetramerization is mediated by the interaction between a coiled-coil of PRKG1 and the leucine/isoleucine zipper of PPP1R12A/MBS, the myosin-binding subunit of the myosin phosphatase By similarity.
Miscellaneous	Exhibit a substrate specificity similar but not identical to that of CAK By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cGMP subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 2 cyclic nucleotide-binding domains. Contains 1 protein kinase domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
Ligand	ATP-binding Nucleotide-binding cGMP cGMP-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Acetylation Disulfide bond Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cGMP binding Inferred from electronic annotation. Source: UniProtKB-KW cGMP-dependent protein kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Select]

Isoform Alpha (identifier: O77676-1)

Also known as: CGK1-alpha;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Beta (identifier: O77676-2)

Also known as: CGK1-beta;

The sequence of this isoform is not available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 671	670	cGMP-dependent protein kinase 1		PRO_0000086116
Regions
	Domain	360 – 619	260	Protein kinase
	Domain	620 – 671	52	AGC-kinase C-terminal
	Nucleotide binding	103 – 220	118	cGMP 1
	Nucleotide binding	221 – 341	121	cGMP 2
	Nucleotide binding	366 – 374	9	ATP By similarity
	Region	2 – 102	101	Dimerization By similarity
	Coiled coil	9 – 44	36	By similarity
Sites
	Active site	484	1	Proton acceptor By similarity
	Binding site	390	1	ATP By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylserine By similarity
	Modified residue	59	1	Phosphothreonine; by autocatalysis By similarity
	Modified residue	515	1	Phosphothreonine By similarity
	Disulfide bond	43		Interchain By similarity
Natural variations
	Natural variant	558	1	F → S

Sequences

	Sequence		Length	Mass (Da)
	Isoform Alpha (CGK1-alpha) [UniParc]. Last modified January 23, 2007. Version 3. Checksum: F8E50992F39C0D81	FASTA	671	76,454
	10 20 30 40 50 60 MSELEEDFAK ILMLKEERIK ELEKRLSEKE EEIQELKRKL HKCQSVLPVP STHIGPRTTR 70 80 90 100 110 120 AQGISAEPQT YRSFHDLRQA FRKFTKFERS KDLIKEAILD NDFMKNLELS QIQEIVDCMY 130 140 150 160 170 180 PVEYGKDSCI IKEGDVGSLA YVMEDGKVEV TKEGVKLCTM GPGKVFGELA ILYNCTRTAT 190 200 210 220 230 240 VKTLVNVKLW AIDRQCFQTI MMRTGLIKHT EYMEFLKSVP TFQSLPEEIL SKLADVLEET 250 260 270 280 290 300 HYENEEYSIR QGARGDTFFI ISKGKVNVTR EDSPSEDPIF LRTLGKGDWF GEKALQGEDV 310 320 330 340 350 360 RTANVIAAEA VTCLVIDRDS FKHLIGGLDD VSNKAYEDAE AKAKYEAEAA FFANLKLSDF 370 380 390 400 410 420 NIIDTLGVGG FGRVELVQLK SEESKTFAMK ILKKRHIVDT RQQEHIRSEK QIMQGAHSDF 430 440 450 460 470 480 IVRLYRTFKD SKYLYMLMEA CLGGELWTIL RDRGSFEDST TRFYTACVVE AFAYLHSKGI 490 500 510 520 530 540 IYRDLKPENL ILDHRGYAKL VDFGFAKKIG FGKKTWTFCG TPEYVAPEII LNKGHDISAD 550 560 570 580 590 600 YWSLGILMYE LLTGSPPFSG PDPMKTYNII LRGIDMIEFP KKIAKNAANL IKKLCRDNPS 610 620 630 640 650 660 ERLGNLKNGV KDIQKHKWFE GFNWEGLRKG TLTPPIIPSV ASPTDTSNFD GFPEDNDEPP 670 PDDNSGWDID F
	Isoform Beta (CGK1-beta) (Sequence not available).	FASTA	–	–

References

[1]	"Analysis of expression of cGMP-dependent protein kinase in rabbit heart cells." Kumar R., Joyner R.W., Komalavilas P., Lincoln T.M. J. Pharmacol. Exp. Ther. 291:967-975(1999) [PubMed: 10565812] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: New Zealand white. Tissue: Heart.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF076969 mRNA. Translation: AAC31192.1.
RefSeq	NP_001075511.1.
UniGene	Ocu.2417
3D structure databases
SMR	O77676. Positions 9-44, 59-337, 325-629, 354-665.
ModBase	Search...
Genome annotation databases
GeneID	100008694.
Phylogenomic databases
eggNOG	maNOG19158.
HOVERGEN	O77676.
Enzyme and pathway databases
BRENDA	2.7.11.12. 255.
Family and domain databases
InterPro	IPR000961. AGC-kinase_C. IPR016232. cGMP-dependant_protein_kinase. IPR002374. cGMP_kinase_core. IPR000595. cNMP_bd. IPR018490. cNMP_bd-like. IPR018488. cNMP_bd_CS. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR014710. RmlC-like_jellyroll. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
Gene3D	G3DSA:2.60.120.10. RmlC-like_jellyroll. 2 hits.
Pfam	PF00027. cNMP_binding. 2 hits. PF00069. Pkinase. 1 hit. [Graphical view]
PIRSF	PIRSF000559. cGMP-dep_kinase. 1 hit.
PRINTS	PR00104. CGMPKINASE.
SMART	SM00100. cNMP. 2 hits. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS00888. CNMP_BINDING_1. False negative. PS00889. CNMP_BINDING_2. 2 hits. PS50042. CNMP_BINDING_3. 2 hits. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KGP1_RABIT

Accession

Primary (citable) accession number: O77676

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 23, 2007
Last modified:	February 9, 2010
This is version 72 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)