Reviewed, UniProtKB/Swiss-Prot O77668 (OREX_PIG)

Names and origin

Protein names

Recommended name:
    Orexin
Alternative name(s):
    Hypocretin
      Short name=Hcrt
Cleaved into the following 2 chains:
    1- Recommended name:
            Orexin-A
        Alternative name(s):
            Hypocretin-1
              Short name=Hcrt1
    2- Recommended name:
            Orexin-B
        Alternative name(s):
            Hypocretin-2
              Short name=Hcrt2

Gene names

Name:	HCRT
Synonyms:	ORX, OX, PPOX

Organism

Sus scrofa (Pig)

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	131 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A binds to both OX1R and OX2R with a high affinity, whereas orexin-B binds only to OX2R with a similar high affinity By similarity.
Subcellular location	Rough endoplasmic reticulum By similarity. Cytoplasmic vesicle By similarity. Cell junction › synapse By similarity. Note: Associated with perikaryal rough endoplasmic reticulum as well as cytoplasmic large granular vesicles at synapses By similarity.
Post-translational modification	Specific enzymatic cleavages at paired basic residues yield the different active peptides.
Sequence similarities	Belongs to the orexin family.

Ontologies

Keywords
Cellular component	Cell junction Cytoplasmic vesicle Endoplasmic reticulum Synapse
Domain	Signal
Molecular function	Neuropeptide
PTM	Amidation Cleavage on pair of basic residues Disulfide bond Pyrrolidone carboxylic acid
Gene Ontology (GO)
Biological process	feeding behavior Inferred from electronic annotation. Source: InterPro neuropeptide signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW cytoplasmic membrane-bounded vesicle Inferred from electronic annotation. Source: UniProtKB-SubCell rough endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell synapse Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 33	33	By similarity
	Peptide	34 – 66	33	Orexin-A		PRO_0000020267
	Peptide	70 – 97	28	Orexin-B		PRO_0000020268
	Propeptide	98 – 131	34			PRO_0000020269
Amino acid modifications
	Modified residue	34	1	Pyrrolidone carboxylic acid By similarity
	Modified residue	66	1	Leucine amide By similarity
	Modified residue	97	1	Methionine amide By similarity
	Disulfide bond	39 ↔ 45		By similarity
	Disulfide bond	40 ↔ 47		By similarity

Sequences

	Sequence		Length	Mass (Da)
	O77668-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 665A74448429AB1F	FASTA	131	13,457
	10 20 30 40 50 60 MNPPFAKVSW ATVTLLLLLL LLPPAVLSPG AAAQPLPDCC RQKTCSCRLY ELLHGAGNHA 70 80 90 100 110 120 AGILTLGKRR PGPPGLQGRL QRLLQASGNH AAGILTMGRR AGAEPAPRLC PGRRCLAAAA 130 SSVAPGGRSG I

References

[1]	"Cloning of porcine prepro-orexin cDNA and effects of an intramuscular injection of synthetic porcine orexin-B on feed intake in young pigs." Dyer C.J., Touchette K.J., Carroll J.A., Allee G.L., Matteri R.L. Domest. Anim. Endocrinol. 16:145-148(1999) [PubMed: 10343916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SYNTHESIS OF OREXIN-B. Tissue: Hypothalamus.
[2]	Guo H.H., Wang L.X. Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[3]	"Linkage and physical mapping of the porcine prepro-orexin gene." Malek M., Marklund S., Dyer C., Matteri R.L., Rothschild M.F. Mamm. Genome 11:342-343(2000) [PubMed: 10754114] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 3-131.
+	Additional computationally mapped references.

Web resources

Protein Spotlight

Qui dort dine - Issue 15 of October 2001

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF075241 mRNA. Translation: AAC26827.1. DQ985365 Genomic DNA. Translation: ABJ15704.1. EF434654 Genomic DNA. Translation: ABO15568.1. EF434655 mRNA. Translation: ABO15569.1. AF169352 Genomic DNA. Translation: AAF24216.1.
RefSeq	NP_999321.1.
UniGene	Ssc.15983
3D structure databases
SMR	O77668. Positions 34-66, 72-97.
ModBase	Search...
Genome annotation databases
Ensembl	ENSSSCT00000018952; ENSSSCP00000018447; ENSSSCG00000017410; Sus scrofa. [Genome view]
GeneID	397305.
KEGG	ssc:397305.
Organism-specific databases
CTD	397305.
Phylogenomic databases
HOVERGEN	O77668.
Family and domain databases
InterPro	IPR001704. Orexin. [Graphical view]
PANTHER	PTHR15173. Orexin. 1 hit.
Pfam	PF02072. Orexin. 1 hit. [Graphical view]
PRINTS	PR01091. OREXINPP.
ProtoNet	Search...

Entry information

Entry name

OREX_PIG

Accession

Primary (citable) accession number: O77668
Secondary accession number(s): Q000Y9, Q9TTA6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1998
Last modified:	January 19, 2010
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)