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Protein

Corticosteroid 11-beta-dehydrogenase isozyme 2

Gene

HSD11B2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of cortisol to the inactive metabolite cortisone. Modulates intracellular glucocorticoid levels, thus protecting the nonselective mineralocorticoid receptor from occupation by glucocorticoids. Affinity towards corticosterone is higher than cortisol or dexamethasone.

Catalytic activityi

An 11-beta-hydroxysteroid + NAD+ = an 11-oxosteroid + NADH.

Enzyme regulationi

Inhibited by glycyrrhetinic acid, carbenoloxone and 11-alpha-OH-progesterone.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei219 – 2191SubstrateBy similarity
Active sitei232 – 2321Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi82 – 11130NADBy similarityAdd
BLAST

GO - Molecular functioni

  1. oxidoreductase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Corticosteroid 11-beta-dehydrogenase isozyme 2 (EC:1.1.1.-)
Alternative name(s):
11-beta-hydroxysteroid dehydrogenase type 2
Short name:
11-DH2
Short name:
11-beta-HSD2
NAD-dependent 11-beta-hydroxysteroid dehydrogenase
Gene namesi
Name:HSD11B2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 404404Corticosteroid 11-beta-dehydrogenase isozyme 2PRO_0000054626Add
BLAST

Proteomic databases

PRIDEiO77667.

Expressioni

Tissue specificityi

Highly expressed in kidney, adrenal and colon; detected at lower levels on lung and liver.

Interactioni

Subunit structurei

Interacts with ligand-free cytoplasmic NR3C2.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000007470.

Structurei

3D structure databases

ProteinModelPortaliO77667.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1028.
HOVERGENiHBG005482.
InParanoidiO77667.
KOiK00071.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O77667-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MESWPWPSGG AWLLVPARAL LQLLRADLRL GRPLLAALAL LAALDWLCQR
60 70 80 90 100
LLPPLAALAV LAATGWIVLS RLARPQRLPV ATRAVLITGC DSGFGNATAK
110 120 130 140 150
KLDTMGFTVL ATVLDLNSPG ALELRACCSS RLKLLQMDLT KPGDISRVLE
160 170 180 190 200
FTKVHTPSTG LWGLVNNAGQ NIFVADAELC PVATFRTCME VNFFGALEMT
210 220 230 240 250
KGLLPLLRRS SGRIVTVSSP AGDMPFPCLA AYGTSKAALA LLMGNFSCEL
260 270 280 290 300
LPWGVKVSII QPACFKTESV KDVHQWEERK QQLLATLPQE LLQAYGEDYI
310 320 330 340 350
EHLNGQFLHS LSQALPDLSP VVDAITDALL AAQPLRRYYP GHGLGLIYFI
360 370 380 390 400
HYYLPEGLRQ RFLQSFFISP YVPRALQAGQ PGLTSARDIA QDQGPRPDPS

PTAQ
Length:404
Mass (Da):43,987
Last modified:November 1, 1998 - v1
Checksum:iE04C2852CFD4D064
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF074706 mRNA. Translation: AAC26137.1.
RefSeqiNP_777067.1. NM_174642.2.
UniGeneiBt.49451.

Genome annotation databases

GeneIDi282434.
KEGGibta:282434.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF074706 mRNA. Translation: AAC26137.1.
RefSeqiNP_777067.1. NM_174642.2.
UniGeneiBt.49451.

3D structure databases

ProteinModelPortaliO77667.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000007470.

Proteomic databases

PRIDEiO77667.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi282434.
KEGGibta:282434.

Organism-specific databases

CTDi3291.

Phylogenomic databases

eggNOGiCOG1028.
HOVERGENiHBG005482.
InParanoidiO77667.
KOiK00071.

Miscellaneous databases

NextBioi20806210.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and expression of the bovine 11beta-hydroxysteroid dehydrogenase type-2."
    Romero D.G., Zhou M.-Y., Gomez-Sanchez C.E.
    J. Steroid Biochem. Mol. Biol. 72:231-237(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
    Tissue: Kidney.

Entry informationi

Entry nameiDHI2_BOVIN
AccessioniPrimary (citable) accession number: O77667
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1998
Last modified: January 7, 2015
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.