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O77667 (DHI2_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Corticosteroid 11-beta-dehydrogenase isozyme 2
EC=1.1.1.-
Alternative name(s):
11-beta-hydroxysteroid dehydrogenase type 2
Short name=11-DH2
Short name=11-beta-HSD2
NAD-dependent 11-beta-hydroxysteroid dehydrogenase
Gene names
Name:HSD11B2
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length404 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the conversion of cortisol to the inactive metabolite cortisone. Modulates intracellular glucocorticoid levels, thus protecting the nonselective mineralocorticoid receptor from occupation by glucocorticoids. Affinity towards corticosterone is higher than cortisol or dexamethasone.

Catalytic activity

An 11-beta-hydroxysteroid + NAD+ = an 11-oxosteroid + NADH.

Enzyme regulation

Inhibited by glycyrrhetinic acid, carbenoloxone and 11-alpha-OH-progesterone.

Subunit structure

Interacts with ligand-free cytoplasmic NR3C2 By similarity.

Subcellular location

Microsome. Endoplasmic reticulum Potential.

Tissue specificity

Highly expressed in kidney, adrenal and colon; detected at lower levels on lung and liver.

Sequence similarities

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
   Cellular componentEndoplasmic reticulum
Microsome
   LigandNAD
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentendoplasmic reticulum

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionnucleotide binding

Inferred from electronic annotation. Source: InterPro

oxidoreductase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 404404Corticosteroid 11-beta-dehydrogenase isozyme 2
PRO_0000054626

Regions

Nucleotide binding82 – 11130NAD By similarity

Sites

Active site2321Proton acceptor By similarity
Binding site2191Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
O77667 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: E04C2852CFD4D064

FASTA40443,987
        10         20         30         40         50         60 
MESWPWPSGG AWLLVPARAL LQLLRADLRL GRPLLAALAL LAALDWLCQR LLPPLAALAV 

        70         80         90        100        110        120 
LAATGWIVLS RLARPQRLPV ATRAVLITGC DSGFGNATAK KLDTMGFTVL ATVLDLNSPG 

       130        140        150        160        170        180 
ALELRACCSS RLKLLQMDLT KPGDISRVLE FTKVHTPSTG LWGLVNNAGQ NIFVADAELC 

       190        200        210        220        230        240 
PVATFRTCME VNFFGALEMT KGLLPLLRRS SGRIVTVSSP AGDMPFPCLA AYGTSKAALA 

       250        260        270        280        290        300 
LLMGNFSCEL LPWGVKVSII QPACFKTESV KDVHQWEERK QQLLATLPQE LLQAYGEDYI 

       310        320        330        340        350        360 
EHLNGQFLHS LSQALPDLSP VVDAITDALL AAQPLRRYYP GHGLGLIYFI HYYLPEGLRQ 

       370        380        390        400 
RFLQSFFISP YVPRALQAGQ PGLTSARDIA QDQGPRPDPS PTAQ

« Hide

References

[1]"Cloning and expression of the bovine 11beta-hydroxysteroid dehydrogenase type-2."
Romero D.G., Zhou M.-Y., Gomez-Sanchez C.E.
J. Steroid Biochem. Mol. Biol. 72:231-237(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
Tissue: Kidney.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF074706 mRNA. Translation: AAC26137.1.
IPIIPI00688380.
RefSeqNP_777067.1. NM_174642.2.
UniGeneBt.49451.

3D structure databases

ProteinModelPortalO77667.
ModBaseSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000007470.

Proteomic databases

PRIDEO77667.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID282434.
KEGGbta:282434.

Organism-specific databases

CTD3291.

Phylogenomic databases

eggNOGCOG1028.
HOVERGENHBG005482.
InParanoidO77667.
KOK00071.
OrthoDBEOG49CQ89.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSPR00081. GDHRDH.
PROSITEPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20806210.

Entry information

Entry nameDHI2_BOVIN
AccessionPrimary (citable) accession number: O77667
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1998
Last modified: April 3, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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