O77649 (UDB20_MACFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-glucuronosyltransferase 2B20
Short name=UDPGT 2B20
EC=2.4.1.17

Gene names

Name:

UGT2B20

Organism

Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	530 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	UDPGTs are of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isozyme has glucuronidating capacity with androgens, such as testosterone, dihydrotestosterone (DHT) and 3-alpha-diol. It is also active on catecholoestrogens including 1,3,5,10-oestratriene-3,4-diol-17-one.
Catalytic activity	UDP-glucuronate + acceptor = UDP + acceptor beta-D-glucuronoside.
Subcellular location	Microsome membrane; Single-pass membrane protein Potential. Endoplasmic reticulum membrane; Single-pass membrane protein Potential.
Sequence similarities	Belongs to the UDP-glycosyltransferase family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glucuronosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 530	507	UDP-glucuronosyltransferase 2B20		PRO_0000036044
Regions
	Transmembrane	495 – 515	21	Helical; Potential
Amino acid modifications
	Glycosylation	65	1	N-linked (GlcNAc...) Potential
	Glycosylation	103	1	N-linked (GlcNAc...) Potential
	Glycosylation	316	1	N-linked (GlcNAc...) Potential
	Glycosylation	483	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	O77649 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: A5EB47F8D517D8DA	FASTA	530	61,225
	10 20 30 40 50 60 MSLKWTSVFL LLQLSCYFSS GSCGKVLVWP TEYSHWINMK TILEELVRRR HEVTVLTSSA 70 80 90 100 110 120 STFVNDSKSS AIKFEVYPTS LTKNDMEDSL MKLLDIWTYS ISNSTFLSYF SKLQELCWEY 130 140 150 160 170 180 YYYSEKLCKD AVLNKKLMTK LKETKFDVIL ADALNPCGEL LAELFNIPFV YSLRFTVGYT 190 200 210 220 230 240 FEKNGGGFLF PPSYVPVVMS ELSDQMTFTE RIKNMIHKLY FDFWFQIHDI KKWDQFYSEV 250 260 270 280 290 300 LGRPTTLFET MRKAEMWLIR TYWDFEFPRP FLPNVDFVGG LHCKPAKPLP KEMEEFVQSS 310 320 330 340 350 360 GENGVVVFSL GSMISNMSEE RANMIASALA QIPQKVLWKF DGKKPNTLGS NTRLYKWLPQ 370 380 390 400 410 420 NDLLGHPKTK AFITHGGTNG IYEAIYHGIP MVGIPLFADQ HDNIVHMKVK GAALSVDIRT 430 440 450 460 470 480 MSSRDLLNAL KSVINEPIYK ENAMKLSRIH HDQPMKPLDR AVFWIEFVMR HKGAKHLRVA 490 500 510 520 530 AHNLTWIQYH SLDVIAFLLA CVAAVIFIIT KCCLFCFRKL AKTGKKKKWD

References

[1]

"Cloning and characterization of a simian UDP-glucuronosyltransferase enzyme UGT2B20, a novel C19 steroid-conjugating protein." Barbier O., Belanger A., Hum D.W. Biochem. J. 337:567-574(1999) [PubMed: 9895303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION. Tissue: Liver and Prostate.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF072223 mRNA. Translation: AAD08808.1.
3D structure databases
ProteinModelPortal	O77649.
SMR	O77649. Positions 286-447.
ModBase	Search...
Protein family/group databases
CAZy	GT1. Glycosyltransferase Family 1.
Proteomic databases
PRIDE	O77649.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG004033.
Family and domain databases
InterPro	IPR002213. UDP_glucos_trans. [Graphical view]
PANTHER	PTHR11926. UDP_glucos_trans. 1 hit.
Pfam	PF00201. UDPGT. 1 hit. [Graphical view]
PROSITE	PS00375. UDPGT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UDB20_MACFA

Accession

Primary (citable) accession number: O77649

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1998
Last modified:	October 19, 2011
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program