O77642 (1433S_SHEEP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
14-3-3 protein sigma

Alternative name(s):
Stratifin

Gene names

Name:

SFN

Organism

Ovis aries (Sheep)

Taxonomic identifier

9940 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Caprinae › Ovis

Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway By similarity.
Subunit structure	Homodimer. Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN. Interacts with KRT17. Interacts with GAB2 and SAMSN1 By similarity. Interacts with SRPK2 By similarity. Interacts with COPS6 By similarity. Interacts with RFWD2; this interaction leads to proteasomal degradation By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity. Secreted By similarity. Note: May be secreted by a non-classical secretory pathway By similarity.
Miscellaneous	14-3-3 proteins have been shown to be PKC activators, but this effect could be non-specific and only due to the acidic nature of the protein.
Sequence similarities	Belongs to the 14-3-3 family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus Secreted
PTM	Phosphoprotein
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 248	248	14-3-3 protein sigma		PRO_0000058645
Sites
	Site	56	1	Interaction with phosphoserine on interacting protein By similarity
	Site	129	1	Interaction with phosphoserine on interacting protein By similarity
Amino acid modifications
	Modified residue	64	1	Phosphoserine By similarity
	Modified residue	216	1	Phosphoserine By similarity
	Modified residue	248	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O77642 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: F1166FB40B23A9B3	FASTA	248	27,849
	10 20 30 40 50 60 MERASLIQKA KLAEQAERYE DMAAFMKSAV EKGEELSCEE RNLLSVAYKN VVGGQRAAWR 70 80 90 100 110 120 VLSSIEQKSN EESSEEKGPE VQEYREKVET ELRGVCDTVL GLLDTHLIKE AGDAESRVFY 130 140 150 160 170 180 LKMKGDYYRY LAEVATGDDK KRIIDSARSA YQEAMDISKK EMPPTNPIRL GLALNFSVFH 190 200 210 220 230 240 YEIANSPEEA ISLAKTTFDE AMADLHTLSE DSYKDSTLIM QLLRDNLTLW TADNAGEEGG EAPEEPQS

References

[1]

"Identification of differentially expressed genes during a wool follicle growth cycle induced by prolactin." Rufaut N.W., Pearson A.J., Nixon A.J., Wheeler T.T., Wilkins R.J. J. Invest. Dermatol. 113:865-872(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: New Zealand Wiltshire. Tissue: Skin.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF071008 mRNA. Translation: AAC24036.1.
RefSeq	NP_001009208.1. NM_001009208.1.
UniGene	Oar.814.
3D structure databases
ProteinModelPortal	O77642.
SMR	O77642. Positions 1-231.
ModBase	Search...
Proteomic databases
PRIDE	O77642.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	443032.
Organism-specific databases
CTD	2810.
Phylogenomic databases
HOVERGEN	HBG050423.
Family and domain databases
Gene3D	1.20.190.20. 1 hit.
InterPro	IPR000308. 14-3-3. IPR023409. 14-3-3_CS. IPR023410. 14-3-3_domain. [Graphical view]
PANTHER	PTHR18860. PTHR18860. 1 hit.
Pfam	PF00244. 14-3-3. 1 hit. [Graphical view]
PIRSF	PIRSF000868. 14-3-3. 1 hit.
PRINTS	PR00305. 1433ZETA.
SMART	SM00101. 14_3_3. 1 hit. [Graphical view]
SUPFAM	SSF48445. 14-3-3. 1 hit.
PROSITE	PS00796. 1433_1. 1 hit. PS00797. 1433_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

1433S_SHEEP

Accession

Primary (citable) accession number: O77642

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	November 1, 1998
Last modified:	April 3, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program