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Protein

14-3-3 protein sigma

Gene

SFN

Organism
Ovis aries (Sheep)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53 (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
14-3-3 protein sigma
Alternative name(s):
Stratifin
Gene namesi
Name:SFN
OrganismiOvis aries (Sheep)
Taxonomic identifieri9940 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeCaprinaeOvis
Proteomesi
  • UP000002356 Componenti: Unplaced

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity
  • Secreted By similarity

  • Note: May be secreted by a non-classical secretory pathway.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000586451 – 24814-3-3 protein sigmaAdd BLAST248

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei5PhosphoserineBy similarity1
Modified residuei74PhosphoserineBy similarity1
Modified residuei248PhosphoserineBy similarity1

Post-translational modificationi

Ubiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation (By similarity).By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PRIDEiO77642.

Interactioni

Subunit structurei

Homodimer. Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29 and VPS35. Interacts with KRT17. Interacts with GAB2 and SAMSN1 (By similarity). Interacts with SRPK2 (By similarity). Interacts with COPS6 (By similarity). Interacts with RFWD2; this interaction leads to proteasomal degradation (By similarity). Interacts with the 'Thr-369' phosphorylated form of DAPK2 (By similarity). Interacts with PI4KB (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei56Interaction with phosphoserine on interacting proteinBy similarity1
Sitei129Interaction with phosphoserine on interacting proteinBy similarity1

Structurei

3D structure databases

ProteinModelPortaliO77642.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 14-3-3 family.Curated

Phylogenomic databases

HOVERGENiHBG050423.
KOiK06644.

Family and domain databases

InterProiIPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR023410. 14-3-3_domain.
[Graphical view]
PANTHERiPTHR18860. PTHR18860. 1 hit.
PfamiPF00244. 14-3-3. 1 hit.
[Graphical view]
PIRSFiPIRSF000868. 14-3-3. 1 hit.
PRINTSiPR00305. 1433ZETA.
SMARTiSM00101. 14_3_3. 1 hit.
[Graphical view]
SUPFAMiSSF48445. SSF48445. 1 hit.
PROSITEiPS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O77642-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERASLIQKA KLAEQAERYE DMAAFMKSAV EKGEELSCEE RNLLSVAYKN
60 70 80 90 100
VVGGQRAAWR VLSSIEQKSN EESSEEKGPE VQEYREKVET ELRGVCDTVL
110 120 130 140 150
GLLDTHLIKE AGDAESRVFY LKMKGDYYRY LAEVATGDDK KRIIDSARSA
160 170 180 190 200
YQEAMDISKK EMPPTNPIRL GLALNFSVFH YEIANSPEEA ISLAKTTFDE
210 220 230 240
AMADLHTLSE DSYKDSTLIM QLLRDNLTLW TADNAGEEGG EAPEEPQS
Length:248
Mass (Da):27,849
Last modified:November 1, 1998 - v1
Checksum:iF1166FB40B23A9B3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF071008 mRNA. Translation: AAC24036.1.
RefSeqiNP_001009208.1. NM_001009208.1.
UniGeneiOar.814.

Genome annotation databases

GeneIDi443032.
KEGGioas:443032.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF071008 mRNA. Translation: AAC24036.1.
RefSeqiNP_001009208.1. NM_001009208.1.
UniGeneiOar.814.

3D structure databases

ProteinModelPortaliO77642.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiO77642.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi443032.
KEGGioas:443032.

Organism-specific databases

CTDi2810.

Phylogenomic databases

HOVERGENiHBG050423.
KOiK06644.

Family and domain databases

InterProiIPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR023410. 14-3-3_domain.
[Graphical view]
PANTHERiPTHR18860. PTHR18860. 1 hit.
PfamiPF00244. 14-3-3. 1 hit.
[Graphical view]
PIRSFiPIRSF000868. 14-3-3. 1 hit.
PRINTSiPR00305. 1433ZETA.
SMARTiSM00101. 14_3_3. 1 hit.
[Graphical view]
SUPFAMiSSF48445. SSF48445. 1 hit.
PROSITEiPS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry namei1433S_SHEEP
AccessioniPrimary (citable) accession number: O77642
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: November 1, 1998
Last modified: October 5, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

14-3-3 proteins have been shown to be PKC activators, but this effect could be non-specific and only due to the acidic nature of the protein.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.