O77633 (ADA10_PIG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Disintegrin and metalloproteinase domain-containing protein 10
Short name=ADAM 10
EC=3.4.24.81

Alternative name(s):
Kuzbanian protein homolog
Mammalian disintegrin-metalloprotease
CD_antigen=CD156c

Gene names

Name:

ADAM10

Organism

Sus scrofa (Pig) [Complete proteome]

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	75 AA.
Sequence status	Fragment.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface By similarity. Controls also the proteolytic processing of Notch By similarity. May regulate the EFNA5-EPHA3 signaling By similarity.
Catalytic activity	Endopeptidase of broad specificity.
Cofactor	Binds 1 zinc ion By similarity.
Subunit structure	Forms a ternary EFNA5-EPHA3-ADAM10 complex mediating EFNA5 extracellular domain shedding by ADAM10 which regulates the EFNA5-EPHA3 complex internalization and function. Interacts with EPHA2 By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein By similarity.
Tissue specificity	Expressed in chondrocytes.
Induction	Up-regulared in chondrocytes culture by interleukin-1 and reduced by retinoic acid.
Sequence similarities	Contains 1 peptidase M12B domain.

Ontologies

Keywords
Biological process	Notch signaling pathway
Cellular component	Membrane
Ligand	Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	Notch signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›75	›75	Disintegrin and metalloproteinase domain-containing protein 10		PRO_0000078205
Regions
	Domain	‹1 – 65	›65	Peptidase M12B
Amino acid modifications
	Glycosylation	48	1	N-linked (GlcNAc...) Potential
	Disulfide bond	8 ↔ 44		By similarity
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	75	1

Sequences

	Sequence		Length	Mass (Da)
	O77633 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: C03B70C901333557	FASTA	75	8,224
	10 20 30 40 50 60 PHDSGTECTP GESKNLGQKE NGNYIMYARA TSGDKLNNNK FSLCSIRNIS QVLEKKRNNC 70 FVESGQPICG NGMVE

References

[1]	"Effects of culture conditions and exposure to catabolic stimulators (IL-1 and retinoic acid) on the expression of matrix metalloproteinases (MMPs) and disintegrin metalloproteinases (ADAMs) by articular cartilage chondrocytes." Flannery C.R., Little C.B., Caterson B., Hughes C.E. Matrix Biol. 18:225-237(1999) [PubMed: 10429942] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF069645 mRNA. Translation: AAC23529.1.
UniGene	Ssc.1114. Ssc.87428.
3D structure databases
ProteinModelPortal	O77633.
ModBase	Search...
Protein-protein interaction databases
STRING	O77633.
Protein family/group databases
MEROPS	M12.210.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
GeneTree	ENSGT00530000063304.
OrthoDB	EOG457562.
Family and domain databases
InterPro	IPR024079. MetalloPept_cat_dom. IPR001590. Peptidase_M12B. [Graphical view]
Gene3D	G3DSA:3.40.390.10. G3DSA:3.40.390.10. 1 hit.
PROSITE	PS50215. ADAM_MEPRO. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ADA10_PIG

Accession

Primary (citable) accession number: O77633

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 28, 2003
Last sequence update:	November 1, 1998
Last modified:	November 16, 2011
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program