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Protein

Glutathione S-transferase 1, isoform B

Gene

GstD1

Organism
Anopheles gambiae (African malaria mosquito)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.By similarity

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei9 – 91GlutathioneBy similarity

GO - Molecular functioni

  1. glutathione transferase activity Source: UniProtKB

GO - Biological processi

  1. glutathione metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase 1, isoform B (EC:2.5.1.18)
Alternative name(s):
AgGst1-alpha
Aggst1-4
GST class-theta
Gene namesi
Name:GstD11 Publication
Synonyms:GST1aImported
ORF Names:AGAP004164
OrganismiAnopheles gambiae (African malaria mosquito)
Taxonomic identifieri7165 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeAnophelinaeAnopheles
ProteomesiUP000007062: Chromosome 2R

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 216216Glutathione S-transferase 1, isoform BPRO_0000283093Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliO77473.
SMRiO77473. Positions 1-211.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 8080GST N-terminalAdd
BLAST
Domaini89 – 210122GST C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni50 – 523Glutathione bindingBy similarity
Regioni64 – 663Glutathione bindingBy similarity

Sequence similaritiesi

Belongs to the GST superfamily. Theta family.Sequence Analysis
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

HOGENOMiHOG000125741.
InParanoidiO77473.
KOiK00799.
OMAiTIANFKL.
OrthoDBiEOG7V1FRJ.
PhylomeDBiO77473.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. Align

Isoform B1 Publication (identifier: O77473-1) [UniParc]FASTAAdd to Basket

Also known as: 1-41 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDFYYLPGSA PCRAVQMTAA AVGVELNLKL TDLMKGEHMK PEFLKLNPQH
60 70 80 90 100
CVPTLVDNGF ALWESRAIMC YLVEKYGKPC NNDSLYPTDP QKRAIVNQRL
110 120 130 140 150
YFDMGTLYQR FGDYYYPQIF EGAPANETNF AKIGEALAFL DTFLEGERFV
160 170 180 190 200
AGGNGYSLAD ISLYATLTTF EVAGYDFSAY VNVLRWYKSM PELIPASDTN
210
RSWAEAARPF FDKVKH
Length:216
Mass (Da):24,492
Last modified:October 5, 2010 - v2
Checksum:i0E764BDFB98038D1
GO
Isoform A1 Publication (identifier: O77462-1) [UniParc]FASTAAdd to Basket

Also known as: 1-31 Publication

The sequence of this isoform can be found in the external entry O77462.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:186
Mass (Da):21,255
GO
Isoform C1 Publication (identifier: Q93112-1) [UniParc]FASTAAdd to Basket

Also known as: 1-51 Publication

The sequence of this isoform can be found in the external entry Q93112.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:209
Mass (Da):23,783
GO
Isoform D1 Publication (identifier: Q93113-1) [UniParc]FASTAAdd to Basket

Also known as: 1-1, 1-61 Publication

, 2-1

The sequence of this isoform can be found in the external entry Q93113.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:209
Mass (Da):23,445
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti58 – 581N → S in AAC79994. (PubMed:9826692)Curated
Sequence conflicti58 – 581N → S in AAC79998. (PubMed:9826692)Curated
Sequence conflicti128 – 1281T → A in AAC79994. (PubMed:9826692)Curated
Sequence conflicti128 – 1281T → A in AAC79998. (PubMed:9826692)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF071160 Genomic DNA. Translation: AAC79994.1.
AF071162 mRNA. Translation: AAC79998.1.
AAAB01008880 Genomic DNA. Translation: EAA44711.1.
RefSeqiXP_313048.1. XM_313048.4.

Genome annotation databases

EnsemblMetazoaiAGAP004164-RC; AGAP004164-PC; AGAP004164. [O77473-1]
GeneIDi1273988.
KEGGiaga:AgaP_AGAP004164.
VectorBaseiAGAP004163. Anopheles gambiae.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF071160 Genomic DNA. Translation: AAC79994.1.
AF071162 mRNA. Translation: AAC79998.1.
AAAB01008880 Genomic DNA. Translation: EAA44711.1.
RefSeqiXP_313048.1. XM_313048.4.

3D structure databases

ProteinModelPortaliO77473.
SMRiO77473. Positions 1-211.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiAGAP004164-RC; AGAP004164-PC; AGAP004164. [O77473-1]
GeneIDi1273988.
KEGGiaga:AgaP_AGAP004164.
VectorBaseiAGAP004163. Anopheles gambiae.

Organism-specific databases

CTDi1273988.

Phylogenomic databases

HOGENOMiHOG000125741.
InParanoidiO77473.
KOiK00799.
OMAiTIANFKL.
OrthoDBiEOG7V1FRJ.
PhylomeDBiO77473.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The role of alternative mRNA splicing in generating heterogeneity within the Anopheles gambiae class I glutathione S-transferase family."
    Ranson H., Collins F.H., Hemingway J.
    Proc. Natl. Acad. Sci. U.S.A. 95:14284-14289(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], ALTERNATIVE SPLICING.
    Strain: ZAN/UImported.
  2. "The genome sequence of the malaria mosquito Anopheles gambiae."
    Holt R.A., Subramanian G.M., Halpern A., Sutton G.G., Charlab R., Nusskern D.R., Wincker P., Clark A.G., Ribeiro J.M.C., Wides R., Salzberg S.L., Loftus B.J., Yandell M.D., Majoros W.H., Rusch D.B., Lai Z., Kraft C.L., Abril J.F.
    , Anthouard V., Arensburger P., Atkinson P.W., Baden H., de Berardinis V., Baldwin D., Benes V., Biedler J., Blass C., Bolanos R., Boscus D., Barnstead M., Cai S., Center A., Chaturverdi K., Christophides G.K., Chrystal M.A.M., Clamp M., Cravchik A., Curwen V., Dana A., Delcher A., Dew I., Evans C.A., Flanigan M., Grundschober-Freimoser A., Friedli L., Gu Z., Guan P., Guigo R., Hillenmeyer M.E., Hladun S.L., Hogan J.R., Hong Y.S., Hoover J., Jaillon O., Ke Z., Kodira C.D., Kokoza E., Koutsos A., Letunic I., Levitsky A.A., Liang Y., Lin J.-J., Lobo N.F., Lopez J.R., Malek J.A., McIntosh T.C., Meister S., Miller J.R., Mobarry C., Mongin E., Murphy S.D., O'Brochta D.A., Pfannkoch C., Qi R., Regier M.A., Remington K., Shao H., Sharakhova M.V., Sitter C.D., Shetty J., Smith T.J., Strong R., Sun J., Thomasova D., Ton L.Q., Topalis P., Tu Z.J., Unger M.F., Walenz B., Wang A.H., Wang J., Wang M., Wang X., Woodford K.J., Wortman J.R., Wu M., Yao A., Zdobnov E.M., Zhang H., Zhao Q., Zhao S., Zhu S.C., Zhimulev I., Coluzzi M., della Torre A., Roth C.W., Louis C., Kalush F., Mural R.J., Myers E.W., Adams M.D., Smith H.O., Broder S., Gardner M.J., Fraser C.M., Birney E., Bork P., Brey P.T., Venter J.C., Weissenbach J., Kafatos F.C., Collins F.H., Hoffman S.L.
    Science 298:129-149(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PEST.

Entry informationi

Entry nameiGST1B_ANOGA
AccessioniPrimary (citable) accession number: O77473
Secondary accession number(s): Q7PH26
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: October 5, 2010
Last modified: January 7, 2015
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.