Reviewed, UniProtKB/Swiss-Prot O77473 (GST1B_ANOGA)

Names and origin

Protein names

Recommended name:
    Glutathione S-transferase 1, isoform B
    EC=2.5.1.18
Alternative name(s):
    GST class-theta
    AgGst1-alpha
    Aggst1-4

Gene names

Name:	GstD1
Synonyms:	GST1a
ORF Names:	AGAP004164

Organism

Anopheles gambiae (African malaria mosquito) [Complete proteome]

Taxonomic identifier

7165 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Nematocera › Culicoidea › Culicidae › Anophelinae › Anopheles

Protein attributes

Sequence length	216 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles By similarity. UniProtKB P30711
Catalytic activity	RX + glutathione = HX + R-S-glutathione. UniProtKB P30711
Enzyme regulation	Inhibited by S-hexylglutathione By similarity.
Subunit structure	Homodimer. Binds one molecule of S-hexylglutathione inhibitor per subunit By similarity.
Sequence similarities	Belongs to the GST superfamily. Theta family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutathione metabolic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	extracellular region Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	glutathione transferase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform B Ref.1 (identifier: O77473-1)

Also known as: 1-4;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform A Ref.1 (identifier: O77462-1)

Also known as: 1-3;

The sequence of this isoform can be found in the external entry O77462-1.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Isoform C Ref.1 (identifier: Q93112-1)

Also known as: 1-5;

The sequence of this isoform can be found in the external entry Q93112-1.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Isoform D Ref.1 (identifier: Q93113-1)

Also known as: 1-1; 1-6; 2-1;

The sequence of this isoform can be found in the external entry Q93113-1.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 216	216	Glutathione S-transferase 1, isoform B		PRO_0000283093
Regions
	Domain	1 – 80	80	GST N-terminal
	Domain	89 – 210	122	GST C-terminal
	Region	6 – 11	6	S-hexylglutathione-binding By similarity
	Region	50 – 53	4	S-hexylglutathione-binding By similarity
	Region	64 – 66	3	S-hexylglutathione-binding By similarity
	Region	207 – 211	5	S-hexylglutathione-binding By similarity
Sites
	Active site	9	1	By similarity
	Binding site	33	1	S-hexylglutathione By similarity
	Binding site	38	1	S-hexylglutathione By similarity
	Binding site	104	1	S-hexylglutathione By similarity
	Binding site	108	1	S-hexylglutathione By similarity
	Binding site	116	1	S-hexylglutathione By similarity

Sequences

	Sequence		Length	Mass (Da)
	Isoform B (1-4) [UniParc]. Last modified November 1, 1998. Version 1. Checksum: BC94517EEC7A7F1E	FASTA	216	24,435
	10 20 30 40 50 60 MDFYYLPGSA PCRAVQMTAA AVGVELNLKL TDLMKGEHMK PEFLKLNPQH CVPTLVDSGF 70 80 90 100 110 120 ALWESRAIMC YLVEKYGKPC NNDSLYPTDP QKRAIVNQRL YFDMGTLYQR FGDYYYPQIF 130 140 150 160 170 180 EGAPANEANF AKIGEALAFL DTFLEGERFV AGGNGYSLAD ISLYATLTTF EVAGYDFSAY 190 200 210 VNVLRWYKSM PELIPASDTN RSWAEAARPF FDKVKH
	Isoform A (1-3). See O77462.	FASTA	–	–
	Isoform C (1-5). See Q93112.	FASTA	–	–
	Isoform D (1-1) (1-6) (2-1). See Q93113.	FASTA	–	–

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The role of alternative mRNA splicing in generating heterogeneity within the Anopheles gambiae class I glutathione S-transferase family." Ranson H., Collins F.H., Hemingway J. Proc. Natl. Acad. Sci. U.S.A. 95:14284-14289(1998) [PubMed: 9826692] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], ALTERNATIVE SPLICING. Strain: ZAN/U.
[2]	"The genome sequence of the malaria mosquito Anopheles gambiae." Holt R.A., Subramanian G.M., Halpern A., Sutton G.G., Charlab R., Nusskern D.R., Wincker P., Clark A.G., Ribeiro J.M.C., Wides R., Salzberg S.L., Loftus B.J., Yandell M.D., Majoros W.H., Rusch D.B., Lai Z., Kraft C.L., Abril J.F. , Anthouard V., Arensburger P., Atkinson P.W., Baden H., de Berardinis V., Baldwin D., Benes V., Biedler J., Blass C., Bolanos R., Boscus D., Barnstead M., Cai S., Center A., Chaturverdi K., Christophides G.K., Chrystal M.A.M., Clamp M., Cravchik A., Curwen V., Dana A., Delcher A., Dew I., Evans C.A., Flanigan M., Grundschober-Freimoser A., Friedli L., Gu Z., Guan P., Guigo R., Hillenmeyer M.E., Hladun S.L., Hogan J.R., Hong Y.S., Hoover J., Jaillon O., Ke Z., Kodira C.D., Kokoza E., Koutsos A., Letunic I., Levitsky A.A., Liang Y., Lin J.-J., Lobo N.F., Lopez J.R., Malek J.A., McIntosh T.C., Meister S., Miller J.R., Mobarry C., Mongin E., Murphy S.D., O'Brochta D.A., Pfannkoch C., Qi R., Regier M.A., Remington K., Shao H., Sharakhova M.V., Sitter C.D., Shetty J., Smith T.J., Strong R., Sun J., Thomasova D., Ton L.Q., Topalis P., Tu Z.J., Unger M.F., Walenz B., Wang A.H., Wang J., Wang M., Wang X., Woodford K.J., Wortman J.R., Wu M., Yao A., Zdobnov E.M., Zhang H., Zhao Q., Zhao S., Zhu S.C., Zhimulev I., Coluzzi M., della Torre A., Roth C.W., Louis C., Kalush F., Mural R.J., Myers E.W., Adams M.D., Smith H.O., Broder S., Gardner M.J., Fraser C.M., Birney E., Bork P., Brey P.T., Venter J.C., Weissenbach J., Kafatos F.C., Collins F.H., Hoffman S.L. Science 298:129-149(2002) [PubMed: 12364791] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PEST.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF071162 mRNA. Translation: AAC79998.1. AF071160 Genomic DNA. Translation: AAC79994.1. AAAB01008880 Genomic DNA. No translation available.
3D structure databases
HSSP	HSSP built from PDB template 1PN9 based on UniProtKB Q93113.
ModBase	Search...
Genome annotation databases
Ensembl	AGAP004164. Anopheles gambiae. [Contig view]
Enzyme and pathway databases
BRENDA	2.5.1.18. 165157.
Family and domain databases
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:1.20.1050.10. GST_C_like. 1 hit. G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GST1B_ANOGA

Accession

Primary (citable) accession number: O77473

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	November 1, 1998
Last modified:	March 3, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)