O77473 (GST1B_ANOGA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione S-transferase 1, isoform B
EC=2.5.1.18

Alternative name(s):
AgGst1-alpha
Aggst1-4
GST class-theta

Gene names

Name:	GstD1
Synonyms:	GST1a
ORF Names:	AGAP004164

Organism

Anopheles gambiae (African malaria mosquito) [Reference proteome]

Taxonomic identifier

7165 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Nematocera › Culicimorpha › Culicoidea › Culicidae › Anophelinae › Anopheles › Cellia › Pyretophorus › gambiae species complex

Protein attributes

Sequence length	216 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles By similarity. UniProtKB P30711
Catalytic activity	RX + glutathione = HX + R-S-glutathione. UniProtKB P30711
Subunit structure	Homodimer By similarity. UniProtKB P30711
Sequence similarities	Belongs to the GST superfamily. Theta family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glutathione metabolic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	extracellular region Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	glutathione transferase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform B Ref.1 (identifier: O77473-1)

Also known as: 1-4;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform A Ref.1 (identifier: O77462-1)

Also known as: 1-3;

The sequence of this isoform can be found in the external entry O77462.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Isoform C Ref.1 (identifier: Q93112-1)

Also known as: 1-5;

The sequence of this isoform can be found in the external entry Q93112.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Isoform D Ref.1 (identifier: Q93113-1)

Also known as: 1-1; 1-6; 2-1;

The sequence of this isoform can be found in the external entry Q93113.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 216	216	Glutathione S-transferase 1, isoform B		PRO_0000283093
Regions
	Domain	1 – 80	80	GST N-terminal
	Domain	89 – 210	122	GST C-terminal
	Region	50 – 52	3	Glutathione binding By similarity
	Region	64 – 66	3	Glutathione binding By similarity
Sites
	Binding site	9	1	Glutathione By similarity
Experimental info
	Sequence conflict	58	1	N → S in AAC79994. Ref.1
	Sequence conflict	58	1	N → S in AAC79998. Ref.1
	Sequence conflict	128	1	T → A in AAC79994. Ref.1
	Sequence conflict	128	1	T → A in AAC79998. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform B (1-4) [UniParc]. Last modified October 5, 2010. Version 2. Checksum: 0E764BDFB98038D1	FASTA	216	24,492
	10 20 30 40 50 60 MDFYYLPGSA PCRAVQMTAA AVGVELNLKL TDLMKGEHMK PEFLKLNPQH CVPTLVDNGF 70 80 90 100 110 120 ALWESRAIMC YLVEKYGKPC NNDSLYPTDP QKRAIVNQRL YFDMGTLYQR FGDYYYPQIF 130 140 150 160 170 180 EGAPANETNF AKIGEALAFL DTFLEGERFV AGGNGYSLAD ISLYATLTTF EVAGYDFSAY 190 200 210 VNVLRWYKSM PELIPASDTN RSWAEAARPF FDKVKH
	Isoform A (1-3) [UniParc]. See O77462.		–	–
	Isoform C (1-5) [UniParc]. See Q93112.		–	–
	Isoform D (1-1) (1-6) (2-1) [UniParc]. See Q93113.		–	–

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The role of alternative mRNA splicing in generating heterogeneity within the Anopheles gambiae class I glutathione S-transferase family." Ranson H., Collins F.H., Hemingway J. Proc. Natl. Acad. Sci. U.S.A. 95:14284-14289(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], ALTERNATIVE SPLICING. Strain: ZAN/U.
[2]	"The genome sequence of the malaria mosquito Anopheles gambiae." Holt R.A., Subramanian G.M., Halpern A., Sutton G.G., Charlab R., Nusskern D.R., Wincker P., Clark A.G., Ribeiro J.M.C., Wides R., Salzberg S.L., Loftus B.J., Yandell M.D., Majoros W.H., Rusch D.B., Lai Z., Kraft C.L., Abril J.F. , Anthouard V., Arensburger P., Atkinson P.W., Baden H., de Berardinis V., Baldwin D., Benes V., Biedler J., Blass C., Bolanos R., Boscus D., Barnstead M., Cai S., Center A., Chaturverdi K., Christophides G.K., Chrystal M.A.M., Clamp M., Cravchik A., Curwen V., Dana A., Delcher A., Dew I., Evans C.A., Flanigan M., Grundschober-Freimoser A., Friedli L., Gu Z., Guan P., Guigo R., Hillenmeyer M.E., Hladun S.L., Hogan J.R., Hong Y.S., Hoover J., Jaillon O., Ke Z., Kodira C.D., Kokoza E., Koutsos A., Letunic I., Levitsky A.A., Liang Y., Lin J.-J., Lobo N.F., Lopez J.R., Malek J.A., McIntosh T.C., Meister S., Miller J.R., Mobarry C., Mongin E., Murphy S.D., O'Brochta D.A., Pfannkoch C., Qi R., Regier M.A., Remington K., Shao H., Sharakhova M.V., Sitter C.D., Shetty J., Smith T.J., Strong R., Sun J., Thomasova D., Ton L.Q., Topalis P., Tu Z.J., Unger M.F., Walenz B., Wang A.H., Wang J., Wang M., Wang X., Woodford K.J., Wortman J.R., Wu M., Yao A., Zdobnov E.M., Zhang H., Zhao Q., Zhao S., Zhu S.C., Zhimulev I., Coluzzi M., della Torre A., Roth C.W., Louis C., Kalush F., Mural R.J., Myers E.W., Adams M.D., Smith H.O., Broder S., Gardner M.J., Fraser C.M., Birney E., Bork P., Brey P.T., Venter J.C., Weissenbach J., Kafatos F.C., Collins F.H., Hoffman S.L. Science 298:129-149(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PEST.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF071160 Genomic DNA. Translation: AAC79994.1. AF071162 mRNA. Translation: AAC79998.1. AAAB01008880 Genomic DNA. Translation: EAA44711.1.
RefSeq	XP_313048.1. XM_313048.4.
3D structure databases
HSSP	HSSP built from PDB template 1PN9 based on UniProtKB Q93113.
ProteinModelPortal	O77473.
SMR	O77473. Positions 1-211.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	AGAP004164-RC; AGAP004164-PC; AGAP004164.
GeneID	1273988.
KEGG	aga:AgaP_AGAP004164.
VectorBase	AGAP004163. Anopheles gambiae.
Organism-specific databases
CTD	1273988.
Phylogenomic databases
HOGENOM	HOG000125741.
KO	K00799.
OMA	TFEVAGY.
OrthoDB	EOG42RBQ8.
PhylomeDB	O77473.
Gene expression databases
ArrayExpress	O77473.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
Pfam	PF02798. GST_N. 1 hit. [Graphical view]
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GST1B_ANOGA

Accession

Primary (citable) accession number: O77473
Secondary accession number(s): Q7PH26

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	October 5, 2010
Last modified:	April 3, 2013
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)