O77460 (IPYR_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inorganic pyrophosphatase
EC=3.6.1.1

Alternative name(s):
Nucleosome-remodeling factor 38 kDa subunit
Pyrophosphate phospho-hydrolase
Short name=PPase

Gene names

Name:	Nurf-38
ORF Names:	CG4634

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of NURF (nucleosome remodeling factor), a complex which catalyzes ATP-dependent nucleosome sliding and facilitates transcription of chromatin. NURF is required for homeotic gene expression, proper larval blood cell development, normal male X chromosome morphology, ecdysteroid signaling and metamorphosis. Nurf-38 may have adapted to deliver pyrophosphatase to chromatin to assist in replication or transcription by efficient removal of the inhibitory metabolite. Ref.1 Ref.5
Catalytic activity	Diphosphate + H2O = 2 phosphate.
Cofactor	Binds 4 magnesium ions per subunit. Other metal ions can support activity, but at a lower rate. Two magnesium ions are required for the activation of the enzyme and are present before substrate binds, two additional magnesium ions form complexes with substrate and product By similarity.
Subunit structure	Component of the NURF complex composed of Caf1, E(bx), Nurf-38 and Iswi.
Subcellular location	Cytoplasm. Nucleus.
Miscellaneous	The ATPase activity of NURF is stimulated by the presence of nucleosomes rather than by free DNA.
Sequence similarities	Belongs to the PPase family.
Sequence caution	The sequence AAC97111.1 differs from that shown. Reason: Erroneous initiation. The sequence AAL68291.1 differs from that shown. Reason: Frameshift at position 18.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Ligand	Magnesium Metal-binding
Molecular function	Chromatin regulator Hydrolase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	ecdysone receptor-mediated signaling pathway Inferred from genetic interaction. Source: FlyBase nucleosome mobilization Inferred from direct assay. Source: FlyBase phosphate-containing compound metabolic process Inferred from electronic annotation. Source: InterPro positive regulation of Notch signaling pathway Inferred from genetic interaction. Source: FlyBase regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from direct assay. Source: FlyBase
Cellular component	NURF complex Inferred from direct assay Ref.1. Source: FlyBase cytoplasm Inferred from direct assay Ref.1. Source: FlyBase
Molecular function	inorganic diphosphatase activity Inferred from direct assay Ref.1. Source: FlyBase magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 338	338	Inorganic pyrophosphatase		PRO_0000137572
Sites
	Metal binding	166	1	Magnesium 1 By similarity
	Metal binding	171	1	Magnesium 1 By similarity
	Metal binding	171	1	Magnesium 2 By similarity
	Metal binding	203	1	Magnesium 1 By similarity
	Binding site	129	1	Diphosphate By similarity
Experimental info
	Mutagenesis	129	1	R → A: Drastic reduction of activity. Ref.1
	Sequence conflict	193 – 194	2	TI → QF in AAC97111. Ref.1
	Sequence conflict	193 – 194	2	TI → QF in AAC97112. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O77460 [UniParc]. Last modified August 31, 2004. Version 3. Checksum: B4B7341CD5A3E5F2	FASTA	338	37,939
	10 20 30 40 50 60 MLAKITRSSF YASRAVGRLS GSIPTSPAAL ASNCRYIQIE RKRTKSHEMA LYETVEKGAK 70 80 90 100 110 120 NSPSYSLYFK NKCGNVISPM HDIPLYANEE KTIYNMVVEV PRWTNAKMEI SLKTPMNPIK 130 140 150 160 170 180 QDIKKGKLRF VANCFPHKGY IWNYGALPQT WENPDHIEPS TGCKGDNDPI DVIEIGYRVA 190 200 210 220 230 240 KRGDVLKVKV LGTIALIDEG ETDWKIIAID VNDPLASKVN DIADVDQYFP GLLRATVEWF 250 260 270 280 290 300 KIYKIPDGKP ENQFAFNGDA KNADFANTII AETHKFWQNL VHQSPASGSI STTNITNRNS 310 320 330 EHVIPKEEAE KILAEAPDGG QVEEVSDTVD TWHFIHLK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Inorganic pyrophosphatase is a component of the Drosophila nucleosome remodeling factor complex." Gdula D.A., Sandaltzopoulos R., Tsukiyama T., Ossipow V., Wu C. Genes Dev. 12:3206-3216(1998) [PubMed: 9784495] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], PARTIAL PROTEIN SEQUENCE, FUNCTION, MUTAGENESIS OF ARG-129.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[5]	"Purification and properties of an ATP-dependent nucleosome remodeling factor." Tsukiyama T., Wu C. Cell 83:1011-1020(1995) [PubMed: 8521501] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF085600 Genomic DNA. Translation: AAC97111.1. Different initiation. AF085601 mRNA. Translation: AAC97112.1. AE013599 Genomic DNA. Translation: AAF47227.2. AY075479 mRNA. Translation: AAL68291.1. Frameshift.
RefSeq	NP_001137758.1. NM_001144286.2. NP_523849.3. NM_079125.3.
UniGene	Dm.1958.
3D structure databases
ProteinModelPortal	O77460.
SMR	O77460. Positions 51-337.
ModBase	Search...
Protein-protein interaction databases
IntAct	O77460. 1 interaction.
MINT	MINT-286916.
STRING	O77460.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0072343; FBpp0072250; FBgn0016687.
GeneID	37922.
KEGG	dme:Dmel_CG4634.
NMPDR	fig|7227.3.peg.7338.
Organism-specific databases
CTD	37922.
FlyBase	FBgn0016687. Nurf-38.
Phylogenomic databases
eggNOG	inNOG04019.
GeneTree	EMGT00050000017751.
InParanoid	O77460.
OMA	STGCKGD.
OrthoDB	EOG4J0ZQZ.
PhylomeDB	O77460.
Gene expression databases
Bgee	O77460.
GermOnline	CG4634. Drosophila melanogaster.
Family and domain databases
InterPro	IPR008162. Pyrophosphatase. [Graphical view]
Gene3D	G3DSA:3.90.80.10. Pyrophosphatase. 1 hit.
KO	K11726.
PANTHER	PTHR10286. Pyrophosphatase. 1 hit.
Pfam	PF00719. Pyrophosphatase. 1 hit. [Graphical view]
SUPFAM	SSF50324. Pyrophosphatase. 1 hit.
PROSITE	PS00387. PPASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	806063.

Entry information

Entry name

IPYR_DROME

Accession

Primary (citable) accession number: O77460
Secondary accession number(s): Q9W150

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	August 31, 2004
Last modified:	January 25, 2012
This is version 107 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project