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Protein

Transcription factor Ken

Gene

ken

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Transcription factor required for terminalia development. Negative regulator of the JAK/STAT pathway: represses JAK/STAT-dependent expression of ventral veins lacking (vvl) in the posterior spiracles.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri500 – 52223C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri528 – 55124C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri567 – 59024C2H2-type 3PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • transcription factor activity, sequence-specific DNA binding Source: UniProtKB

GO - Biological processi

  • copulation Source: FlyBase
  • female analia development Source: UniProtKB
  • female genitalia development Source: UniProtKB
  • insemination Source: FlyBase
  • male analia development Source: UniProtKB
  • male genitalia development Source: UniProtKB
  • negative regulation of JAK-STAT cascade Source: UniProtKB
  • phagocytosis Source: FlyBase
  • regulation of JAK-STAT cascade Source: FlyBase
  • regulation of transcription, DNA-templated Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor Ken
Alternative name(s):
Protein Ken and Barbie
Gene namesi
Name:ken
ORF Names:CG5575
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0011236. ken.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Flies show low mating success and reduced copulation duration. Men and female genitalia often remain inside the body, and genitalia and analia are missing in some homozygous flies. These flies are named after the famous dolls who also lack these 'features'.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 601601Transcription factor KenPRO_0000046925Add
BLAST

Proteomic databases

PaxDbiO77459.
PRIDEiO77459.

Expressioni

Tissue specificityi

Expressed from stage 5 in two rather faint stripes at positions of 64% (anterior domain; AD) and 17% (posterior domain; PD) egg length. During early gastrulation, at stage 6, these two stripes become more evident and detectable at the region posterior to the cephalic furrow and in the hindgut primordium. The AD disappears as gastrulation proceeds, while the PD remains. At stage 15, the AD appears again in the foregut, and PD expression in the hindgut and anal pad. In imaginal disks, it is ubiquitously expressed in both males and females in genital and eye-antennal disks. Not expressed in the brain. In genital disks, it is expressed along the margin of the anterior bulbus in males, while in females it is expressed in the posterior compartment along the anterior-posterior border, with medial expansion in the most posterior region.3 Publications

Developmental stagei

Expressed both maternally and zygotically. Highly expressed in embryos and pupae, and at lower level in larvae. In adults, it is expressed at higher level in females.3 Publications

Gene expression databases

BgeeiFBgn0011236.
GenevisibleiO77459. DM.

Interactioni

Protein-protein interaction databases

BioGridi63378. 3 interactions.
DIPiDIP-22018N.
IntActiO77459. 1 interaction.
MINTiMINT-981522.
STRINGi7227.FBpp0072038.

Structurei

3D structure databases

ProteinModelPortaliO77459.
SMRiO77459. Positions 498-589.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini33 – 10169BTBPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi245 – 25814His-richAdd
BLAST
Compositional biasi257 – 27822Asn-richAdd
BLAST

Sequence similaritiesi

Contains 1 BTB (POZ) domain.PROSITE-ProRule annotation
Contains 3 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri500 – 52223C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri528 – 55124C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri567 – 59024C2H2-type 3PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00530000063676.
InParanoidiO77459.
OMAiCERSGGH.
OrthoDBiEOG091G051W.
PhylomeDBiO77459.

Family and domain databases

Gene3Di3.30.160.60. 2 hits.
InterProiIPR000210. BTB/POZ_dom.
IPR011333. SKP1/BTB/POZ.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00651. BTB. 1 hit.
[Graphical view]
SMARTiSM00225. BTB. 1 hit.
SM00355. ZnF_C2H2. 3 hits.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O77459-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKEFQRMLML QYSKHGECIL KEIGAAFRGE HPADLTIVCE NKVKLHAHKL
60 70 80 90 100
VLAAASPLIR NLLEDTHLSD CSTTVYFPDV NATYFKFLLD FLYSGQTCIT
110 120 130 140 150
SRDVNYLHDL LLLLQIKSDS WKTTDSAYLS SKCGGLRDRA DRRKQQYTSP
160 170 180 190 200
QNLEPDQTLK YEVDSVDESR NAADFSSAFN SNDNCESAAE CERSGGHNNK
210 220 230 240 250
EEDEDDCTHK DNKSDKDTDE IVNLSNAPPS GTSGSNSNIS TSSNHQQQQH
260 270 280 290 300
HHHHHHNHNN NNNNNNNNSS SSTINPVNLS LDLRTKSENS ASRTLGSGSD
310 320 330 340 350
HSGIDLAVTA SESTKRKGLF FDSHKDVMKP LSDGSDINSS PENYVVTPHR
360 370 380 390 400
KRRPGFHNTQ SDNQPFTSYP HSLLEELRLA KSTTSPISGF GSEKNMLAHL
410 420 430 440 450
EDGALNGDTL TPDRKHLLEA QRNRAQSPEI PMHLGPQFVY QWQSNQNAAM
460 470 480 490 500
SAMPNLQSRL SSLSHISLNL DHPEGRSGSA SGSGANLAGS NTHASSVREY
510 520 530 540 550
RCEYCGKQFG MSWNLKTHLR VHTGEKPFAC RLCVAMFKQK AHLLKHLCSV
560 570 580 590 600
HRNVITTTNG ADTENRYSCC FCSMCFESVQ ELVRHLSGHH NNLLLTKNLR

E
Length:601
Mass (Da):67,033
Last modified:November 1, 1998 - v1
Checksum:iC9AAA437DAD48FE8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ012576 mRNA. Translation: CAA10062.1.
AB010260 mRNA. Translation: BAA32683.1.
AB010261 Genomic DNA. Translation: BAA32687.1.
AE013599 Genomic DNA. Translation: AAF47084.1.
AF181647 mRNA. Translation: AAD55433.1.
PIRiT00119.
RefSeqiNP_523833.1. NM_079109.3.
UniGeneiDm.2792.

Genome annotation databases

EnsemblMetazoaiFBtr0072129; FBpp0072038; FBgn0011236.
GeneIDi37785.
KEGGidme:Dmel_CG5575.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ012576 mRNA. Translation: CAA10062.1.
AB010260 mRNA. Translation: BAA32683.1.
AB010261 Genomic DNA. Translation: BAA32687.1.
AE013599 Genomic DNA. Translation: AAF47084.1.
AF181647 mRNA. Translation: AAD55433.1.
PIRiT00119.
RefSeqiNP_523833.1. NM_079109.3.
UniGeneiDm.2792.

3D structure databases

ProteinModelPortaliO77459.
SMRiO77459. Positions 498-589.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi63378. 3 interactions.
DIPiDIP-22018N.
IntActiO77459. 1 interaction.
MINTiMINT-981522.
STRINGi7227.FBpp0072038.

Proteomic databases

PaxDbiO77459.
PRIDEiO77459.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0072129; FBpp0072038; FBgn0011236.
GeneIDi37785.
KEGGidme:Dmel_CG5575.

Organism-specific databases

CTDi37785.
FlyBaseiFBgn0011236. ken.

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00530000063676.
InParanoidiO77459.
OMAiCERSGGH.
OrthoDBiEOG091G051W.
PhylomeDBiO77459.

Miscellaneous databases

GenomeRNAii37785.
PROiO77459.

Gene expression databases

BgeeiFBgn0011236.
GenevisibleiO77459. DM.

Family and domain databases

Gene3Di3.30.160.60. 2 hits.
InterProiIPR000210. BTB/POZ_dom.
IPR011333. SKP1/BTB/POZ.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00651. BTB. 1 hit.
[Graphical view]
SMARTiSM00225. BTB. 1 hit.
SM00355. ZnF_C2H2. 3 hits.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKEN_DROME
AccessioniPrimary (citable) accession number: O77459
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: November 1, 1998
Last modified: September 7, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.