O77457 (T23O_ANOGA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan 2,3-dioxygenase
Short name=TDO
EC=1.13.11.11

Alternative name(s):
Tryptamin 2,3-dioxygenase
Tryptophan oxygenase
Short name=TO
Short name=TRPO
Tryptophan pyrrolase
Tryptophanase

Gene names

ORF Names:

AGAP002721

Organism

Anopheles gambiae (African malaria mosquito)

Taxonomic identifier

7165 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Nematocera › Culicoidea › Culicidae › Anophelinae › Anopheles

Protein attributes

Sequence length	392 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring By similarity.
Catalytic activity	L-tryptophan + O2 = N-formyl-L-kynurenine.
Cofactor	Binds 2 heme groups per tetramer By similarity.
Pathway	Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the tryptophan 2,3-dioxygenase family.

Ontologies

Keywords
Biological process	Tryptophan catabolism
Ligand	Heme Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	tryptophan catabolic process to kynurenine Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	heme binding Inferred from sequence or structural similarity. Source: UniProtKB tryptophan 2,3-dioxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 392	392	Tryptophan 2,3-dioxygenase		PRO_0000360078
Regions
	Region	27 – 31	5	Substrate binding By similarity
	Region	57 – 61	5	Substrate binding By similarity
Sites
	Metal binding	313	1	Iron (heme axial ligand) By similarity
	Binding site	128	1	Substrate By similarity
	Binding site	135	1	Heme By similarity
	Binding site	328	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O77457 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 9A2790C0D27FFBD9	FASTA	392	45,402
	10 20 30 40 50 60 MSCPMRSGFV DSVQGGHHLG SEAGMLYGEY LMLDKVLSAQ RMLSVEGKKP VHDEHLFIVT 70 80 90 100 110 120 HQAYELWFKQ IIFELDSIRD LFSTEHIEES RTLEILKRLN RIVMILKLLV DQVPILETMT 130 140 150 160 170 180 PLDFMDFRDY LSPASGFQSL QFRLLENKLG VKSEHRVKYN QKYTEVFASD PGAIERIGTT 190 200 210 220 230 240 ETEPSLADLV QKWLERTPGL EQDGFNFWGK FQESVEQLLA EQEASAMSEE HENVREYRLM 250 260 270 280 290 300 DIDKRREVYK SIFDAQVHDA LVARGERRFT HKALQGAIMI TFYRDEPRFS QPHQLLMLLM 310 320 330 340 350 360 DIDSLITKWR YNHVIMVQRM IGSQQLGTGG SSGYQYLRST LSDRYKVFLD LFNLSTFLIP 370 380 390 RQSIPPLTNE MQKALNLAWG SPAHFARNGS LH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The tryptophan oxygenase gene of Anopheles gambiae." Mukabayire O., Cornel A.J., Dotson E.M., Collins F.H., Besansky N.J. Insect Biochem. Mol. Biol. 26:525-528(1996) [PubMed: 8969464] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The genome sequence of the malaria mosquito Anopheles gambiae." Holt R.A., Subramanian G.M., Halpern A., Sutton G.G., Charlab R., Nusskern D.R., Wincker P., Clark A.G., Ribeiro J.M.C., Wides R., Salzberg S.L., Loftus B.J., Yandell M.D., Majoros W.H., Rusch D.B., Lai Z., Kraft C.L., Abril J.F. , Anthouard V., Arensburger P., Atkinson P.W., Baden H., de Berardinis V., Baldwin D., Benes V., Biedler J., Blass C., Bolanos R., Boscus D., Barnstead M., Cai S., Center A., Chaturverdi K., Christophides G.K., Chrystal M.A.M., Clamp M., Cravchik A., Curwen V., Dana A., Delcher A., Dew I., Evans C.A., Flanigan M., Grundschober-Freimoser A., Friedli L., Gu Z., Guan P., Guigo R., Hillenmeyer M.E., Hladun S.L., Hogan J.R., Hong Y.S., Hoover J., Jaillon O., Ke Z., Kodira C.D., Kokoza E., Koutsos A., Letunic I., Levitsky A.A., Liang Y., Lin J.-J., Lobo N.F., Lopez J.R., Malek J.A., McIntosh T.C., Meister S., Miller J.R., Mobarry C., Mongin E., Murphy S.D., O'Brochta D.A., Pfannkoch C., Qi R., Regier M.A., Remington K., Shao H., Sharakhova M.V., Sitter C.D., Shetty J., Smith T.J., Strong R., Sun J., Thomasova D., Ton L.Q., Topalis P., Tu Z.J., Unger M.F., Walenz B., Wang A.H., Wang J., Wang M., Wang X., Woodford K.J., Wortman J.R., Wu M., Yao A., Zdobnov E.M., Zhang H., Zhao Q., Zhao S., Zhu S.C., Zhimulev I., Coluzzi M., della Torre A., Roth C.W., Louis C., Kalush F., Mural R.J., Myers E.W., Adams M.D., Smith H.O., Broder S., Gardner M.J., Fraser C.M., Birney E., Bork P., Brey P.T., Venter J.C., Weissenbach J., Kafatos F.C., Collins F.H., Hoffman S.L. Science 298:129-149(2002) [PubMed: 12364791] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PEST.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L76432 mRNA. Translation: AAC27663.1. L76433 Genomic DNA. Translation: AAC27659.1. AAAB01008859 Genomic DNA. Translation: EAA08131.2.
RefSeq	XP_312204.2. XM_312204.2.
3D structure databases
HSSP	HSSP built from PDB template 1YW0 based on UniProtKB Q8PDA8.
ProteinModelPortal	O77457.
ModBase	Search...
Protein-protein interaction databases
STRING	O77457.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	AGAP002721-RA; AGAP002721-PA; AGAP002721.
GeneID	1273245.
KEGG	aga:AgaP_AGAP002721.
Organism-specific databases
CTD	1273245.
Phylogenomic databases
eggNOG	inNOG07042.
GeneTree	EMGT00050000008199.
HOGENOM	HBG647485.
InParanoid	O77457.
OMA	KLLVDQV.
OrthoDB	EOG4NCJVC.
PhylomeDB	O77457.
Family and domain databases
InterPro	IPR004981. Trp_2_3_dOase. [Graphical view]
KO	K00453.
PANTHER	PTHR10138. Trp_2_3_dOase. 1 hit.
Pfam	PF03301. Trp_dioxygenase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T23O_ANOGA

Accession

Primary (citable) accession number: O77457
Secondary accession number(s): Q7QCN1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	November 1, 1998
Last modified:	December 14, 2011
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)