Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Telomerase reverse transcriptase

Gene

TERT

Organism
Tetrahymena thermophila
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. It elongates telomeres. It is a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme (By similarity).By similarity

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi618 – 6181Magnesium; catalyticPROSITE-ProRule annotation
Metal bindingi815 – 8151Magnesium; catalyticPROSITE-ProRule annotation
Metal bindingi816 – 8161Magnesium; catalyticPROSITE-ProRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, RNA-directed DNA polymerase, Transferase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Telomerase reverse transcriptase (EC:2.7.7.49)
Alternative name(s):
Telomerase catalytic subunit
Telomerase subunit P133
Gene namesi
Name:TERT
OrganismiTetrahymena thermophila
Taxonomic identifieri5911 [NCBI]
Taxonomic lineageiEukaryotaAlveolataCiliophoraIntramacronucleataOligohymenophoreaHymenostomatidaTetrahymeninaTetrahymenidaeTetrahymena

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus, Telomere

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11171117Telomerase reverse transcriptasePRO_0000054929Add
BLAST

Interactioni

Protein-protein interaction databases

DIPiDIP-60200N.

Structurei

Secondary structure

1
1117
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi17 – 2610Combined sources
Beta strandi32 – 354Combined sources
Helixi36 – 427Combined sources
Helixi54 – 629Combined sources
Beta strandi64 – 696Combined sources
Helixi73 – 753Combined sources
Helixi89 – 9911Combined sources
Turni100 – 1023Combined sources
Helixi107 – 1093Combined sources
Beta strandi113 – 1153Combined sources
Beta strandi118 – 1203Combined sources
Turni129 – 1324Combined sources
Helixi134 – 14310Combined sources
Helixi145 – 15410Combined sources
Beta strandi156 – 1627Combined sources
Beta strandi165 – 1728Combined sources
Helixi174 – 1818Combined sources
Beta strandi188 – 1903Combined sources
Beta strandi229 – 2313Combined sources
Beta strandi243 – 2453Combined sources
Helixi281 – 29111Combined sources
Helixi301 – 32020Combined sources
Helixi323 – 3308Combined sources
Helixi337 – 34812Combined sources
Helixi356 – 36611Combined sources
Helixi367 – 3693Combined sources
Helixi373 – 38715Combined sources
Helixi390 – 3923Combined sources
Helixi395 – 41016Combined sources
Helixi419 – 4235Combined sources
Helixi428 – 4303Combined sources
Turni441 – 4433Combined sources
Helixi445 – 46622Combined sources
Helixi468 – 4758Combined sources
Beta strandi476 – 4838Combined sources
Beta strandi488 – 4925Combined sources
Helixi493 – 50917Combined sources
Turni510 – 5167Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2B2AX-ray2.22A/B/C2-191[»]
2R4GX-ray1.71A254-519[»]
5C9HX-ray3.00A/B217-516[»]
ProteinModelPortaliO77448.
SMRiO77448. Positions 13-191, 274-506.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO77448.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini517 – 881365Reverse transcriptasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 reverse transcriptase domain.PROSITE-ProRule annotation

Family and domain databases

InterProiIPR000477. RT_dom.
IPR021891. Telomerase_RBD.
IPR003545. Telomerase_RT.
[Graphical view]
PANTHERiPTHR12066:SF0. PTHR12066:SF0. 2 hits.
PfamiPF00078. RVT_1. 1 hit.
PF12009. Telomerase_RBD. 1 hit.
[Graphical view]
PRINTSiPR01365. TELOMERASERT.
SMARTiSM00975. Telomerase_RBD. 1 hit.
[Graphical view]
PROSITEiPS50878. RT_POL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O77448-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQKINNINNN KQMLTRKEDL LTVLKQISAL KYVSNLYEFL LATEKIVQTS
60 70 80 90 100
ELDTQFQEFL TTTIIASEQN LVENYKQKYN QPNFSQLTIK QVIDDSIILL
110 120 130 140 150
GNKQNYVQQI GTTTIGFYVE YENINLSRQT LYSSNFRNLL NIFGEEDFKY
160 170 180 190 200
FLIDFLVFTK VEQNGYLQVA GVCLNQYFSV QVKQKKWYKN NFNMNGKATS
210 220 230 240 250
NNNQNNANLS NEKKQENQYI YPEIQRSQIF YCNHMGREPG VFKSSFFNYS
260 270 280 290 300
EIKKGFQFKV IQEKLQGRQF INSDKIKPDH PQTIIKKTLL KEYQSKNFSC
310 320 330 340 350
QEERDLFLEF TEKIVQNFHN INFNYLLKKF CKLPENYQSL KSQVKQIVQS
360 370 380 390 400
ENKANQQSCE NLFNSLYDTE ISYKQITNFL RQIIQNCVPN QLLGKKNFKV
410 420 430 440 450
FLEKLYEFVQ MKRFENQKVL DYICFMDVFD VEWFVDLKNQ KFTQKRKYIS
460 470 480 490 500
DKRKILGDLI VFIINKIVIP VLRYNFYITE KHKEGSQIFY YRKPIWKLVS
510 520 530 540 550
KLTIVKLEEE NLEKVEEKLI PEDSFQKYPQ GKLRIIPKKG SFRPIMTFLR
560 570 580 590 600
KDKQKNIKLN LNQILMDSQL VFRNLKDMLG QKIGYSVFDN KQISEKFAQF
610 620 630 640 650
IEKWKNKGRP QLYYVTLDIK KCYDSIDQMK LLNFFNQSDL IQDTYFINKY
660 670 680 690 700
LLFQRNKRPL LQIQQTNNLN SAMEIEEEKI NKKPFKMDNI NFPYYFNLKE
710 720 730 740 750
RQIAYSLYDD DDQILQKGFK EIQSDDRPFI VINQDKPRCI TKDIIHNHLK
760 770 780 790 800
HISQYNVISF NKVKFRQKRG IPQGLNISGV LCSFYFGKLE EEYTQFLKNA
810 820 830 840 850
EQVNGSINLL MRLTDDYLFI SDSQQNALNL IVQLQNCANN NGFMFNDQKI
860 870 880 890 900
TTNFQFPQED YNLEHFKISV QNECQWIGKS IDMNTLEIKS IQKQTQQEIN
910 920 930 940 950
QTINVAISIK NLKSQLKNKL RSLFLNQLID YFNPNINSFE GLCRQLYHHS
960 970 980 990 1000
KATVMKFYPF MTKLFQIDLK KSKQYSVQYG KENTNENFLK DILYYTVEDV
1010 1020 1030 1040 1050
CKILCYLQFE DEINSNIKEI FKNLYSWIMW DIIVSYLKKK KQFKGYLNKL
1060 1070 1080 1090 1100
LQKIRKSRFF YLKEGCKSLQ LILSQQKYQL NKKELEAIEF IDLNNLIQDI
1110
KTLIPKISAK SNQQNTN
Length:1,117
Mass (Da):133,318
Last modified:November 1, 1998 - v1
Checksum:iE5AF15E86B0F0CD8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF062652 Genomic DNA. Translation: AAC39135.1.
AF061284 mRNA. Translation: AAC39140.1.
PIRiT14891.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF062652 Genomic DNA. Translation: AAC39135.1.
AF061284 mRNA. Translation: AAC39140.1.
PIRiT14891.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2B2AX-ray2.22A/B/C2-191[»]
2R4GX-ray1.71A254-519[»]
5C9HX-ray3.00A/B217-516[»]
ProteinModelPortaliO77448.
SMRiO77448. Positions 13-191, 274-506.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60200N.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiO77448.

Family and domain databases

InterProiIPR000477. RT_dom.
IPR021891. Telomerase_RBD.
IPR003545. Telomerase_RT.
[Graphical view]
PANTHERiPTHR12066:SF0. PTHR12066:SF0. 2 hits.
PfamiPF00078. RVT_1. 1 hit.
PF12009. Telomerase_RBD. 1 hit.
[Graphical view]
PRINTSiPR01365. TELOMERASERT.
SMARTiSM00975. Telomerase_RBD. 1 hit.
[Graphical view]
PROSITEiPS50878. RT_POL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Telomerase reverse transcriptase genes identified in Tetrahymena thermophila and Oxytricha trifallax."
    Bryan T.M., Sperger J.M., Chapman K.B., Cech T.R.
    Proc. Natl. Acad. Sci. U.S.A. 95:8479-8484(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: B7.
  2. "The reverse transcriptase component of the Tetrahymena telomerase ribonucleoprotein complex."
    Collins K., Gandhi L.
    Proc. Natl. Acad. Sci. U.S.A. 95:8485-8490(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiTERT_TETTH
AccessioniPrimary (citable) accession number: O77448
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: May 11, 2016
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.