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O77438

- FRIZ3_DROME

UniProt

O77438 - FRIZ3_DROME

Protein

Frizzled-3

Gene

fz3

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 2 (02 May 2002)
      Previous versions | rss
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    Functioni

    Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. Required to coordinate the cytoskeletons of epidermal cells to produce a parallel array of cuticular hairs and bristles.2 Publications

    GO - Molecular functioni

    1. G-protein coupled receptor activity Source: UniProtKB-KW
    2. PDZ domain binding Source: RefGenome
    3. transmembrane signaling receptor activity Source: FlyBase
    4. Wnt-activated receptor activity Source: RefGenome
    5. Wnt-protein binding Source: FlyBase

    GO - Biological processi

    1. brain development Source: RefGenome
    2. canonical Wnt signaling pathway Source: RefGenome
    3. establishment or maintenance of cell polarity Source: UniProtKB
    4. gonad development Source: RefGenome
    5. signal transduction Source: FlyBase
    6. vasculature development Source: RefGenome

    Keywords - Molecular functioni

    Developmental protein, G-protein coupled receptor, Receptor, Transducer

    Keywords - Biological processi

    Wnt signaling pathway

    Enzyme and pathway databases

    SignaLinkiO77438.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Frizzled-3
    Short name:
    dFz3
    Gene namesi
    Name:fz3
    ORF Names:CG16785
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome X

    Organism-specific databases

    FlyBaseiFBgn0027343. fz3.

    Subcellular locationi

    GO - Cellular componenti

    1. cell projection Source: RefGenome
    2. cytoplasm Source: RefGenome
    3. integral component of membrane Source: UniProtKB
    4. plasma membrane Source: RefGenome

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Chaini20 – 581562Frizzled-3PRO_0000013013Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi40 ↔ 101PROSITE-ProRule annotation
    Disulfide bondi48 ↔ 94PROSITE-ProRule annotation
    Glycosylationi54 – 541N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi85 ↔ 123PROSITE-ProRule annotation
    Disulfide bondi112 ↔ 153PROSITE-ProRule annotation
    Disulfide bondi116 ↔ 141PROSITE-ProRule annotation
    Glycosylationi206 – 2061N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiO77438.

    Expressioni

    Tissue specificityi

    Wing, leg and eye imaginal disks. In embryos, expressed is seen in brain, proventriculus, Malpighian tubules, anal plate and visceral mesoderm of parasegment 8.

    Developmental stagei

    Expressed in embryos from stage 11 and in larvae.

    Gene expression databases

    BgeeiO77438.

    Interactioni

    Protein-protein interaction databases

    BioGridi57591. 4 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliO77438.
    SMRiO77438. Positions 40-154, 215-518.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini20 – 237218ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini259 – 27012CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini292 – 32130ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini343 – 35917CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini381 – 39313ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini415 – 44228CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini464 – 48825ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini510 – 58172CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei238 – 25821Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei271 – 29121Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei322 – 34221Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei360 – 38021Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei394 – 41421Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei443 – 46321Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei489 – 50921Helical; Name=7Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini35 – 156122FZPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi579 – 5813PDZ-binding

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi403 – 4108Poly-Leu

    Domaini

    The FZ domain is involved in binding with Wnt ligands.By similarity

    Sequence similaritiesi

    Contains 1 FZ (frizzled) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG331834.
    InParanoidiO77438.
    KOiK04690.
    OrthoDBiEOG7X6M0F.
    PhylomeDBiO77438.

    Family and domain databases

    Gene3Di1.10.2000.10. 1 hit.
    InterProiIPR000539. Frizzled.
    IPR015526. Frizzled/SFRP.
    IPR020067. Frizzled_dom.
    IPR026554. FZD3_invertebrates.
    IPR017981. GPCR_2-like.
    [Graphical view]
    PANTHERiPTHR11309. PTHR11309. 1 hit.
    PTHR11309:SF84. PTHR11309:SF84. 1 hit.
    PfamiPF01534. Frizzled. 1 hit.
    PF01392. Fz. 1 hit.
    [Graphical view]
    PRINTSiPR00489. FRIZZLED.
    SMARTiSM00063. FRI. 1 hit.
    [Graphical view]
    SUPFAMiSSF63501. SSF63501. 1 hit.
    PROSITEiPS50038. FZ. 1 hit.
    PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O77438-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYAASILILH LTWAVATIAA NGAGHNGPVA SGAGPNGLQC QPIAVSACQG    50
    LGYNMTALPN LAGHTNQLEA ELQIAKLVPL IESGCSRRAR FLLCSSLFPL 100
    CTPDVPRPVA ACKLLCETVR GECMENAPPE LMELWPSFLN CDGLPQPEKH 150
    ELCMQIPQEV AVPGGSPSGP PTTGSPGVED HPQTYRFWKS GASPTSDLAG 200
    VLCPQNFSGS PFNPEECVPQ CQRDAFHTSS QKKTSETLIL GLSAVCFVLT 250
    LFALVTFWAE PTRFGYPERP VLFLCLCYNL FSVCYLERIV FHNQARMHDV 300
    ELQGRLMRPG CLLTPPCLAS YITTSYLSLC AASWWLIFAL CFYLSSHKKW 350
    SSEALEKRSG LFHVLAWVPP LAPPIAALLL EKVRPSELTG MCYAPGFVEL 400
    PALVLLLLGL YFTLRASRSL LSLQQQLQPT LAHHRFGQIR KRFVLFSLLY 450
    FAPTTAGVVA ALCERYADSV PSCSTPDDCL SPTPLSAWPA LVRIFFQLVG 500
    GTLTGLWVWS RKTCESYRNR LGASGTPTSS LMNQSKAAGA LPKKHLYTSG 550
    KSMLPTGGIT PLYAGISFHN VPVYNPNQSR V 581
    Length:581
    Mass (Da):63,252
    Last modified:May 2, 2002 - v2
    Checksum:i07A8BBFF2A9E3F93
    GO

    Sequence cautioni

    The sequence CAA20896.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti56 – 561T → I in BAA84677. (PubMed:10498678)Curated
    Sequence conflicti192 – 1921A → V in BAA84677. (PubMed:10498678)Curated
    Sequence conflicti276 – 2761L → P in BAA84677. (PubMed:10498678)Curated
    Sequence conflicti304 – 3041G → W in AAF45547. (PubMed:10731132)Curated
    Sequence conflicti304 – 3041G → W in AAM50295. (PubMed:10731132)Curated
    Sequence conflicti376 – 3761A → V in BAA84677. (PubMed:10498678)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB018565 mRNA. Translation: BAA84677.1.
    AF195242 mRNA. Translation: AAF63250.1.
    AE014298 Genomic DNA. Translation: AAF45547.1.
    AL031583 Genomic DNA. Translation: CAA20896.1. Sequence problems.
    AL132792 Genomic DNA. No translation available.
    AY119641 mRNA. Translation: AAM50295.1.
    PIRiT13484.
    RefSeqiNP_525035.2. NM_080296.2.
    UniGeneiDm.1697.

    Genome annotation databases

    GeneIDi31023.
    KEGGidme:Dmel_CG16785.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB018565 mRNA. Translation: BAA84677.1 .
    AF195242 mRNA. Translation: AAF63250.1 .
    AE014298 Genomic DNA. Translation: AAF45547.1 .
    AL031583 Genomic DNA. Translation: CAA20896.1 . Sequence problems.
    AL132792 Genomic DNA. No translation available.
    AY119641 mRNA. Translation: AAM50295.1 .
    PIRi T13484.
    RefSeqi NP_525035.2. NM_080296.2.
    UniGenei Dm.1697.

    3D structure databases

    ProteinModelPortali O77438.
    SMRi O77438. Positions 40-154, 215-518.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 57591. 4 interactions.

    Protein family/group databases

    GPCRDBi Search...

    Proteomic databases

    PaxDbi O77438.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 31023.
    KEGGi dme:Dmel_CG16785.

    Organism-specific databases

    CTDi 31023.
    FlyBasei FBgn0027343. fz3.

    Phylogenomic databases

    eggNOGi NOG331834.
    InParanoidi O77438.
    KOi K04690.
    OrthoDBi EOG7X6M0F.
    PhylomeDBi O77438.

    Enzyme and pathway databases

    SignaLinki O77438.

    Miscellaneous databases

    GenomeRNAii 31023.
    NextBioi 771559.

    Gene expression databases

    Bgeei O77438.

    Family and domain databases

    Gene3Di 1.10.2000.10. 1 hit.
    InterProi IPR000539. Frizzled.
    IPR015526. Frizzled/SFRP.
    IPR020067. Frizzled_dom.
    IPR026554. FZD3_invertebrates.
    IPR017981. GPCR_2-like.
    [Graphical view ]
    PANTHERi PTHR11309. PTHR11309. 1 hit.
    PTHR11309:SF84. PTHR11309:SF84. 1 hit.
    Pfami PF01534. Frizzled. 1 hit.
    PF01392. Fz. 1 hit.
    [Graphical view ]
    PRINTSi PR00489. FRIZZLED.
    SMARTi SM00063. FRI. 1 hit.
    [Graphical view ]
    SUPFAMi SSF63501. SSF63501. 1 hit.
    PROSITEi PS50038. FZ. 1 hit.
    PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Dfrizzled-3, a new Drosophila Wnt receptor, acting as an attenuator of Wingless signaling in wingless hypomorphic mutants."
      Sato A., Kojima T., Ui-Tei K., Miyata Y., Saigo K.
      Development 126:4421-4430(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    2. "The Wingless target gene Dfz3 encodes a new member of the Drosophila Frizzled family."
      Sivasankaran R., Calleja M., Morata G., Basler K.
      Mech. Dev. 91:427-431(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    3. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    4. Cited for: GENOME REANNOTATION.
      Strain: Berkeley.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Oregon-R.
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Berkeley.
      Tissue: Embryo.

    Entry informationi

    Entry nameiFRIZ3_DROME
    AccessioniPrimary (citable) accession number: O77438
    Secondary accession number(s): Q540W1
    , Q9I7Z7, Q9NIU0, Q9U902, Q9W5D5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 2, 2002
    Last sequence update: May 2, 2002
    Last modified: October 1, 2014
    This is version 119 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3