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Protein

Frizzled-3

Gene

fz3

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. Required to coordinate the cytoskeletons of epidermal cells to produce a parallel array of cuticular hairs and bristles.2 Publications

GO - Molecular functioni

  • G-protein coupled receptor activity Source: GO_Central
  • transmembrane signaling receptor activity Source: FlyBase
  • Wnt-activated receptor activity Source: GO_Central
  • Wnt-protein binding Source: FlyBase

GO - Biological processi

  • canonical Wnt signaling pathway Source: GO_Central
  • establishment or maintenance of cell polarity Source: UniProtKB
  • G-protein coupled receptor signaling pathway Source: FlyBase
  • multicellular organism development Source: UniProtKB-KW
  • non-canonical Wnt signaling pathway Source: GO_Central
  • signal transduction Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Wnt signaling pathway

Enzyme and pathway databases

SignaLinkiO77438.

Names & Taxonomyi

Protein namesi
Recommended name:
Frizzled-3
Short name:
dFz3
Gene namesi
Name:fz3
ORF Names:CG16785
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0027343. fz3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini20 – 237ExtracellularSequence analysisAdd BLAST218
Transmembranei238 – 258Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini259 – 270CytoplasmicSequence analysisAdd BLAST12
Transmembranei271 – 291Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini292 – 321ExtracellularSequence analysisAdd BLAST30
Transmembranei322 – 342Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini343 – 359CytoplasmicSequence analysisAdd BLAST17
Transmembranei360 – 380Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini381 – 393ExtracellularSequence analysisAdd BLAST13
Transmembranei394 – 414Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini415 – 442CytoplasmicSequence analysisAdd BLAST28
Transmembranei443 – 463Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini464 – 488ExtracellularSequence analysisAdd BLAST25
Transmembranei489 – 509Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini510 – 581CytoplasmicSequence analysisAdd BLAST72

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000001301320 – 581Frizzled-3Add BLAST562

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi40 ↔ 101PROSITE-ProRule annotation
Disulfide bondi48 ↔ 94PROSITE-ProRule annotation
Glycosylationi54N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi85 ↔ 123PROSITE-ProRule annotation
Disulfide bondi112 ↔ 153PROSITE-ProRule annotation
Disulfide bondi116 ↔ 141PROSITE-ProRule annotation
Glycosylationi206N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO77438.
PRIDEiO77438.

Expressioni

Tissue specificityi

Wing, leg and eye imaginal disks. In embryos, expressed is seen in brain, proventriculus, Malpighian tubules, anal plate and visceral mesoderm of parasegment 8.

Developmental stagei

Expressed in embryos from stage 11 and in larvae.

Gene expression databases

BgeeiFBgn0027343.
GenevisibleiO77438. DM.

Interactioni

GO - Molecular functioni

  • Wnt-protein binding Source: FlyBase

Protein-protein interaction databases

BioGridi57591. 4 interactors.
STRINGi7227.FBpp0111841.

Structurei

3D structure databases

ProteinModelPortaliO77438.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini35 – 156FZPROSITE-ProRule annotationAdd BLAST122

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi579 – 581PDZ-binding3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi403 – 410Poly-Leu8

Domaini

The FZ domain is involved in binding with Wnt ligands.By similarity

Sequence similaritiesi

Contains 1 FZ (frizzled) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3577. Eukaryota.
ENOG410XRC8. LUCA.
InParanoidiO77438.
KOiK04690.
OrthoDBiEOG091G04PG.
PhylomeDBiO77438.

Family and domain databases

Gene3Di1.10.2000.10. 1 hit.
InterProiIPR000539. Frizzled.
IPR015526. Frizzled/SFRP.
IPR020067. Frizzled_dom.
IPR026554. FZD3_invertebrates.
IPR017981. GPCR_2-like.
[Graphical view]
PANTHERiPTHR11309. PTHR11309. 1 hit.
PTHR11309:SF84. PTHR11309:SF84. 1 hit.
PfamiPF01534. Frizzled. 1 hit.
PF01392. Fz. 1 hit.
[Graphical view]
PRINTSiPR00489. FRIZZLED.
SMARTiSM00063. FRI. 1 hit.
SM01330. Frizzled. 1 hit.
[Graphical view]
SUPFAMiSSF63501. SSF63501. 1 hit.
PROSITEiPS50038. FZ. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O77438-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYAASILILH LTWAVATIAA NGAGHNGPVA SGAGPNGLQC QPIAVSACQG
60 70 80 90 100
LGYNMTALPN LAGHTNQLEA ELQIAKLVPL IESGCSRRAR FLLCSSLFPL
110 120 130 140 150
CTPDVPRPVA ACKLLCETVR GECMENAPPE LMELWPSFLN CDGLPQPEKH
160 170 180 190 200
ELCMQIPQEV AVPGGSPSGP PTTGSPGVED HPQTYRFWKS GASPTSDLAG
210 220 230 240 250
VLCPQNFSGS PFNPEECVPQ CQRDAFHTSS QKKTSETLIL GLSAVCFVLT
260 270 280 290 300
LFALVTFWAE PTRFGYPERP VLFLCLCYNL FSVCYLERIV FHNQARMHDV
310 320 330 340 350
ELQGRLMRPG CLLTPPCLAS YITTSYLSLC AASWWLIFAL CFYLSSHKKW
360 370 380 390 400
SSEALEKRSG LFHVLAWVPP LAPPIAALLL EKVRPSELTG MCYAPGFVEL
410 420 430 440 450
PALVLLLLGL YFTLRASRSL LSLQQQLQPT LAHHRFGQIR KRFVLFSLLY
460 470 480 490 500
FAPTTAGVVA ALCERYADSV PSCSTPDDCL SPTPLSAWPA LVRIFFQLVG
510 520 530 540 550
GTLTGLWVWS RKTCESYRNR LGASGTPTSS LMNQSKAAGA LPKKHLYTSG
560 570 580
KSMLPTGGIT PLYAGISFHN VPVYNPNQSR V
Length:581
Mass (Da):63,252
Last modified:May 2, 2002 - v2
Checksum:i07A8BBFF2A9E3F93
GO

Sequence cautioni

The sequence CAA20896 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti56T → I in BAA84677 (PubMed:10498678).Curated1
Sequence conflicti192A → V in BAA84677 (PubMed:10498678).Curated1
Sequence conflicti276L → P in BAA84677 (PubMed:10498678).Curated1
Sequence conflicti304G → W in AAF45547 (PubMed:10731132).Curated1
Sequence conflicti304G → W in AAM50295 (PubMed:10731132).Curated1
Sequence conflicti376A → V in BAA84677 (PubMed:10498678).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB018565 mRNA. Translation: BAA84677.1.
AF195242 mRNA. Translation: AAF63250.1.
AE014298 Genomic DNA. Translation: AAF45547.1.
AL031583 Genomic DNA. Translation: CAA20896.1. Sequence problems.
AL132792 Genomic DNA. No translation available.
AY119641 mRNA. Translation: AAM50295.1.
PIRiT13484.
RefSeqiNP_001284760.1. NM_001297831.1.
NP_525035.2. NM_080296.3.
UniGeneiDm.1697.

Genome annotation databases

GeneIDi31023.
KEGGidme:Dmel_CG16785.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB018565 mRNA. Translation: BAA84677.1.
AF195242 mRNA. Translation: AAF63250.1.
AE014298 Genomic DNA. Translation: AAF45547.1.
AL031583 Genomic DNA. Translation: CAA20896.1. Sequence problems.
AL132792 Genomic DNA. No translation available.
AY119641 mRNA. Translation: AAM50295.1.
PIRiT13484.
RefSeqiNP_001284760.1. NM_001297831.1.
NP_525035.2. NM_080296.3.
UniGeneiDm.1697.

3D structure databases

ProteinModelPortaliO77438.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi57591. 4 interactors.
STRINGi7227.FBpp0111841.

Protein family/group databases

GPCRDBiSearch...

Proteomic databases

PaxDbiO77438.
PRIDEiO77438.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi31023.
KEGGidme:Dmel_CG16785.

Organism-specific databases

CTDi31023.
FlyBaseiFBgn0027343. fz3.

Phylogenomic databases

eggNOGiKOG3577. Eukaryota.
ENOG410XRC8. LUCA.
InParanoidiO77438.
KOiK04690.
OrthoDBiEOG091G04PG.
PhylomeDBiO77438.

Enzyme and pathway databases

SignaLinkiO77438.

Miscellaneous databases

GenomeRNAii31023.
PROiO77438.

Gene expression databases

BgeeiFBgn0027343.
GenevisibleiO77438. DM.

Family and domain databases

Gene3Di1.10.2000.10. 1 hit.
InterProiIPR000539. Frizzled.
IPR015526. Frizzled/SFRP.
IPR020067. Frizzled_dom.
IPR026554. FZD3_invertebrates.
IPR017981. GPCR_2-like.
[Graphical view]
PANTHERiPTHR11309. PTHR11309. 1 hit.
PTHR11309:SF84. PTHR11309:SF84. 1 hit.
PfamiPF01534. Frizzled. 1 hit.
PF01392. Fz. 1 hit.
[Graphical view]
PRINTSiPR00489. FRIZZLED.
SMARTiSM00063. FRI. 1 hit.
SM01330. Frizzled. 1 hit.
[Graphical view]
SUPFAMiSSF63501. SSF63501. 1 hit.
PROSITEiPS50038. FZ. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFRIZ3_DROME
AccessioniPrimary (citable) accession number: O77438
Secondary accession number(s): Q540W1
, Q9I7Z7, Q9NIU0, Q9U902, Q9W5D5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2002
Last sequence update: May 2, 2002
Last modified: November 30, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.