Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ribokinase

Gene

EG:115C2.1

Organism
Drosophila melanogaster (Fruit fly)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the phosphorylation of ribose at O-5 in a reaction requiring ATP and magnesium. The resulting D-ribose-5-phosphate can then be used either for sythesis of nucleotides, histidine, and tryptophan, or as a component of the pentose phosphate pathway.UniRule annotation

Catalytic activityi

ATP + D-ribose = ADP + D-ribose 5-phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Requires a divalent cation, most likely magnesium in vivo, as an electrophilic catalyst to aid phosphoryl group transfer. It is the chelate of the metal and the nucleotide that is the actual substrate.UniRule annotation

Enzyme regulationi

Activated by a monovalent cation that binds near, but not in, the active site. The most likely occupant of the site in vivo is potassium. Ion binding induces a conformational change that may alter substrate affinity.UniRule annotation

Pathwayi: D-ribose degradation

This protein is involved in step 2 of the subpathway that synthesizes D-ribose 5-phosphate from beta-D-ribopyranose.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Ribokinase (EG:115C2.1), Ribokinase (CG17010)
This subpathway is part of the pathway D-ribose degradation, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-ribose 5-phosphate from beta-D-ribopyranose, the pathway D-ribose degradation and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei142SubstrateUniRule annotation1
Binding sitei184ATPUniRule annotation1
Metal bindingi253PotassiumUniRule annotation1
Metal bindingi255Potassium; via carbonyl oxygenUniRule annotation1
Active sitei259Proton acceptorUniRule annotation1
Binding sitei259SubstrateUniRule annotation1
Metal bindingi291Potassium; via carbonyl oxygenUniRule annotation1
Metal bindingi294Potassium; via carbonyl oxygenUniRule annotation1
Metal bindingi296Potassium; via carbonyl oxygenUniRule annotation1
Metal bindingi300PotassiumUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi221 – 226ATPUniRule annotation6
Nucleotide bindingi258 – 259ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotation, Transferase

Keywords - Biological processi

Carbohydrate metabolismUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding, PotassiumUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00916; UER00889.

Names & Taxonomyi

Protein namesi
Recommended name:
RibokinaseUniRule annotation (EC:2.7.1.15UniRule annotation)
Short name:
RKUniRule annotation
Gene namesi
Name:EG:115C2.1Imported
Synonyms:DreddImported
ORF Names:CG13369Imported, Dmel_CG13369Imported
OrganismiDrosophila melanogaster (Fruit fly)Imported
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0025640. CG13369.

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Nucleus UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation, NucleusUniRule annotation

Expressioni

Gene expression databases

BgeeiFBgn0025640.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

DIPiDIP-20885N.
MINTiMINT-1028274.
STRINGi7227.FBpp0070110.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 303PfkBInterPro annotationAdd BLAST300

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni13 – 15Substrate bindingUniRule annotation3
Regioni41 – 45Substrate bindingUniRule annotation5

Sequence similaritiesi

Belongs to the carbohydrate kinase PfkB family. Ribokinase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG2855. Eukaryota.
COG0524. LUCA.
GeneTreeiENSGT00390000005743.
KOiK00852.
OMAiNAIIVYP.
OrthoDBiEOG091G08OZ.

Family and domain databases

CDDicd01174. ribokinase. 1 hit.
Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_01987. Ribokinase. 1 hit.
InterProiIPR002173. Carboh/pur_kinase_PfkB_CS.
IPR011877. D_ribokin.
IPR011611. PfkB_dom.
IPR002139. Ribo/fructo_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF00294. PfkB. 1 hit.
[Graphical view]
PRINTSiPR00990. RIBOKINASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
PROSITEiPS00584. PFKB_KINASES_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O77425-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQTEVLVFG SAIIDFISYT TRLPKAGETL HGHRFQIGYG GKGANQCVAA
60 70 80 90 100
ARQGSRTALV AKLGADTFGS DYLRHLREER VNVNHVEQLA EETTGVAQIA
110 120 130 140 150
VSDGGENNII IVVGANNRLS SCDVSSAKAL FQEAKVLVCQ LETPVEATLT
160 170 180 190 200
ALRAFRGVSI VNAAPAMADT PPELLQLASI FCVNESEAAL MTQMPDIGNI
210 220 230 240 250
EHAEDAVGKL IAAGANTVII TLGKLGAVFG SADSKGVCQH VAAPSVPPEK
260 270 280 290 300
VVDTTGAGDA FIGALAHNLA RHPTRKLEEH IAAACAVASQ SVQLPGTQSS

FPHA
Length:304
Mass (Da):31,626
Last modified:November 1, 1998 - v1
Checksum:iBD051438EC4D3813
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014298 Genomic DNA. Translation: AAF45527.1.
BT021233 mRNA. Translation: AAX33381.1.
AL031581 Genomic DNA. Translation: CAA20884.1.
PIRiT13380.
RefSeqiNP_569850.1. NM_130494.4.
UniGeneiDm.31030.

Genome annotation databases

EnsemblMetazoaiFBtr0070115; FBpp0070110; FBgn0025640.
GeneIDi31007.
KEGGidme:Dmel_CG13369.
UCSCiCG13369-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014298 Genomic DNA. Translation: AAF45527.1.
BT021233 mRNA. Translation: AAX33381.1.
AL031581 Genomic DNA. Translation: CAA20884.1.
PIRiT13380.
RefSeqiNP_569850.1. NM_130494.4.
UniGeneiDm.31030.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-20885N.
MINTiMINT-1028274.
STRINGi7227.FBpp0070110.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0070115; FBpp0070110; FBgn0025640.
GeneIDi31007.
KEGGidme:Dmel_CG13369.
UCSCiCG13369-RA. d. melanogaster.

Organism-specific databases

FlyBaseiFBgn0025640. CG13369.

Phylogenomic databases

eggNOGiKOG2855. Eukaryota.
COG0524. LUCA.
GeneTreeiENSGT00390000005743.
KOiK00852.
OMAiNAIIVYP.
OrthoDBiEOG091G08OZ.

Enzyme and pathway databases

UniPathwayiUPA00916; UER00889.

Miscellaneous databases

ChiTaRSiCG13369. fly.
GenomeRNAii31007.

Gene expression databases

BgeeiFBgn0025640.

Family and domain databases

CDDicd01174. ribokinase. 1 hit.
Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_01987. Ribokinase. 1 hit.
InterProiIPR002173. Carboh/pur_kinase_PfkB_CS.
IPR011877. D_ribokin.
IPR011611. PfkB_dom.
IPR002139. Ribo/fructo_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF00294. PfkB. 1 hit.
[Graphical view]
PRINTSiPR00990. RIBOKINASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
PROSITEiPS00584. PFKB_KINASES_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiO77425_DROME
AccessioniPrimary (citable) accession number: O77425
Entry historyi
Integrated into UniProtKB/TrEMBL: November 1, 1998
Last sequence update: November 1, 1998
Last modified: November 2, 2016
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.