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Protein

Ribokinase

Gene

EG:115C2.1

Organism
Drosophila melanogaster (Fruit fly)
Status
Unreviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the phosphorylation of ribose at O-5 in a reaction requiring ATP and magnesium. The resulting D-ribose-5-phosphate can then be used either for sythesis of nucleotides, histidine, and tryptophan, or as a component of the pentose phosphate pathway.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Catalytic activityi

ATP + D-ribose = ADP + D-ribose 5-phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Requires a divalent cation, most likely magnesium in vivo, as an electrophilic catalyst to aid phosphoryl group transfer. It is the chelate of the metal and the nucleotide that is the actual substrate.UniRule annotation

Enzyme regulationi

Activated by a monovalent cation that binds near, but not in, the active site. The most likely occupant of the site in vivo is potassium. Ion binding induces a conformational change that may alter substrate affinity.UniRule annotation

Pathwayi: D-ribose degradation

This protein is involved in step 2 of the subpathway that synthesizes D-ribose 5-phosphate from beta-D-ribopyranose.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Ribokinase (EG:115C2.1), Ribokinase (Dmel\CG17010)
This subpathway is part of the pathway D-ribose degradation, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-ribose 5-phosphate from beta-D-ribopyranose, the pathway D-ribose degradation and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei142SubstrateUniRule annotation1
Binding sitei184ATPUniRule annotation1
Metal bindingi253PotassiumUniRule annotation1
Metal bindingi255Potassium; via carbonyl oxygenUniRule annotation1
Active sitei259Proton acceptorUniRule annotation1
Binding sitei259SubstrateUniRule annotation1
Metal bindingi291Potassium; via carbonyl oxygenUniRule annotation1
Metal bindingi294Potassium; via carbonyl oxygenUniRule annotation1
Metal bindingi296Potassium; via carbonyl oxygenUniRule annotation1
Metal bindingi300PotassiumUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi221 – 226ATPUniRule annotation6
Nucleotide bindingi258 – 259ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinaseUniRule annotation, Transferase
Biological processCarbohydrate metabolismUniRule annotation
LigandATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding, PotassiumUniRule annotation

Enzyme and pathway databases

ReactomeiR-DME-71336 Pentose phosphate pathway (hexose monophosphate shunt)
UniPathwayiUPA00916; UER00889

Names & Taxonomyi

Protein namesi
Recommended name:
RibokinaseUniRule annotation (EC:2.7.1.15UniRule annotation)
Short name:
RKUniRule annotation
Gene namesi
Name:EG:115C2.1Imported
Synonyms:Dmel\CG13369Imported, DreddImported
ORF Names:CG13369Imported, Dmel_CG13369Imported
OrganismiDrosophila melanogaster (Fruit fly)Imported
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0025640 CG13369

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

CytoplasmUniRule annotation, NucleusUniRule annotation

PTM / Processingi

Proteomic databases

PRIDEiO77425

Expressioni

Gene expression databases

BgeeiFBgn0025640

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

DIPiDIP-20885N
IntActiO77425, 1 interactor
STRINGi7227.FBpp0070110

Structurei

3D structure databases

SMRiO77425
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 303PfkBInterPro annotationAdd BLAST300

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni13 – 15Substrate bindingUniRule annotation3
Regioni41 – 45Substrate bindingUniRule annotation5

Sequence similaritiesi

Belongs to the carbohydrate kinase PfkB family. Ribokinase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG2855 Eukaryota
COG0524 LUCA
GeneTreeiENSGT00390000005743
KOiK00852
OMAiNADHVIS
OrthoDBiEOG091G08OZ

Family and domain databases

CDDicd01174 ribokinase, 1 hit
Gene3Di3.40.1190.20, 1 hit
HAMAPiMF_01987 Ribokinase, 1 hit
InterProiView protein in InterPro
IPR002173 Carboh/pur_kinase_PfkB_CS
IPR011877 D_ribokin
IPR011611 PfkB_dom
IPR002139 Ribo/fructo_kinase
IPR029056 Ribokinase-like
PfamiView protein in Pfam
PF00294 PfkB, 1 hit
PRINTSiPR00990 RIBOKINASE
SUPFAMiSSF53613 SSF53613, 1 hit
PROSITEiView protein in PROSITE
PS00584 PFKB_KINASES_2, 1 hit

Sequencei

Sequence statusi: Complete.

O77425-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQTEVLVFG SAIIDFISYT TRLPKAGETL HGHRFQIGYG GKGANQCVAA
60 70 80 90 100
ARQGSRTALV AKLGADTFGS DYLRHLREER VNVNHVEQLA EETTGVAQIA
110 120 130 140 150
VSDGGENNII IVVGANNRLS SCDVSSAKAL FQEAKVLVCQ LETPVEATLT
160 170 180 190 200
ALRAFRGVSI VNAAPAMADT PPELLQLASI FCVNESEAAL MTQMPDIGNI
210 220 230 240 250
EHAEDAVGKL IAAGANTVII TLGKLGAVFG SADSKGVCQH VAAPSVPPEK
260 270 280 290 300
VVDTTGAGDA FIGALAHNLA RHPTRKLEEH IAAACAVASQ SVQLPGTQSS

FPHA
Length:304
Mass (Da):31,626
Last modified:November 1, 1998 - v1
Checksum:iBD051438EC4D3813
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014298 Genomic DNA Translation: AAF45527.1
BT021233 mRNA Translation: AAX33381.1
AL031581 Genomic DNA Translation: CAA20884.1
PIRiT13380
RefSeqiNP_569850.1, NM_130494.4
UniGeneiDm.31030

Genome annotation databases

EnsemblMetazoaiFBtr0070115; FBpp0070110; FBgn0025640
GeneIDi31007
KEGGidme:Dmel_CG13369
UCSCiCG13369-RA d. melanogaster

Similar proteinsi

Entry informationi

Entry nameiO77425_DROME
AccessioniPrimary (citable) accession number: O77425
Entry historyiIntegrated into UniProtKB/TrEMBL: November 1, 1998
Last sequence update: November 1, 1998
Last modified: May 23, 2018
This is version 157 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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